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The map label for this gene is hemB [H]

Identifier: 84622022

GI number: 84622022

Start: 396854

End: 397846

Strand: Direct

Name: hemB [H]

Synonym: XOO_0365

Alternate gene names: 84622022

Gene position: 396854-397846 (Clockwise)

Preceding gene: 84622021

Following gene: 84622025

Centisome position: 8.03

GC content: 64.35

Gene sequence:

>993_bases
ATGGCCCACCCGCACTATCGTCCCCGCCGCATGCGCCACGATGCGTTTTCGCGCCGGTTGATGCGCGAAAACACGCTGAC
CACCGATGACCTGATCTGGCCGGTGTTCGTGCACGCCTTGTCCGGCCGCGCGCCGATTGCGTCGATGCCGGGCGTGGAAC
GCCTGTCGCTGGAGGAGTTGCTGCGCGAAGCGGAAAACGCGCTGGAACTGGGCATCCCGGTGATGGATCTGTTTCCGGTC
ATCGACCCGGCCGGCAAGAGCCTGGACGCTGCCGAAGCCTGGAACCCGGACGGCCTGGCGCAGCGCGCGGTACGTGCGTT
GAAGTCGCGCTTTCCGGAGCTTGGGGTGATGACCGACGTGGCGCTGGACCCGTACACCACCCACGGGCAGGACGGCATCA
TCGATGACAAGGGCTATGTGCTCAACGAGATCACCGTCGAGGCCCTGGTCAAGCAATCGTTGTCGCATGCGCATGCGGGC
GTGGACATCATTTCGCCTTCGGACATGATGGACGGCCGCATCGGTGCGATCCGCCGCGCGCTCGATGCCGACGATCATCT
CAACGTGCGGATCATGGCGTATTCGGCCAAGTATGCCTCGGCGTTCTACGGCCCGTTCCGCGATGCCGTGGGCAGCGCCG
GCAATCTTGGCAAGGCCGACAAGACCACCTATCAAATGGACCCGGCCAACGGTGACGAAGCCTTGCGCGAGATCGCCCTG
GATCTGGAAGAAGGCGCGGACATGGTGATGGTCAAGCCGGGCATGCCGTATCTGGATGTGCTGCGCCGGGTGAAGGAACA
ATTCCGCGTGCCGACCTTTGCGTATCAGGTCAGCGGCGAGTACGCGATGCTCAAGGCGGCCTCGGCCAATGGCTGGCTGG
ATGAGCGCAAGTGCGTGCTCGAGGCGTTGATGGCCTTCAAGCGTGCCGGTGCCGATGGCGTGCTGACCTATTTCGCCCCG
CAAGTGGCGCGCTGGTTGCGGGAAACGCGCTGA

Upstream 100 bases:

>100_bases
AACAGCCCGCGTGCCGTTGCAGCGCTGCGACACTGCGTCGCAGCGCGTCCTGCGCAGGATGGCGGGCGTAAACTGATGCG
CTGTCTACAGGAGCCTTGTC

Downstream 100 bases:

>100_bases
GCGCGAGCGCCTGCGGCCATGTCGCCGCAGGTGCATCTTAGAGAGGCTAACAAAACGTAGCGAGCAATCGTCAGGTGGGT
GCGGACCGCGCGCTCAGAAC

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MAHPHYRPRRMRHDAFSRRLMRENTLTTDDLIWPVFVHALSGRAPIASMPGVERLSLEELLREAENALELGIPVMDLFPV
IDPAGKSLDAAEAWNPDGLAQRAVRALKSRFPELGVMTDVALDPYTTHGQDGIIDDKGYVLNEITVEALVKQSLSHAHAG
VDIISPSDMMDGRIGAIRRALDADDHLNVRIMAYSAKYASAFYGPFRDAVGSAGNLGKADKTTYQMDPANGDEALREIAL
DLEEGADMVMVKPGMPYLDVLRRVKEQFRVPTFAYQVSGEYAMLKAASANGWLDERKCVLEALMAFKRAGADGVLTYFAP
QVARWLRETR

Sequences:

>Translated_330_residues
MAHPHYRPRRMRHDAFSRRLMRENTLTTDDLIWPVFVHALSGRAPIASMPGVERLSLEELLREAENALELGIPVMDLFPV
IDPAGKSLDAAEAWNPDGLAQRAVRALKSRFPELGVMTDVALDPYTTHGQDGIIDDKGYVLNEITVEALVKQSLSHAHAG
VDIISPSDMMDGRIGAIRRALDADDHLNVRIMAYSAKYASAFYGPFRDAVGSAGNLGKADKTTYQMDPANGDEALREIAL
DLEEGADMVMVKPGMPYLDVLRRVKEQFRVPTFAYQVSGEYAMLKAASANGWLDERKCVLEALMAFKRAGADGVLTYFAP
QVARWLRETR
>Mature_329_residues
AHPHYRPRRMRHDAFSRRLMRENTLTTDDLIWPVFVHALSGRAPIASMPGVERLSLEELLREAENALELGIPVMDLFPVI
DPAGKSLDAAEAWNPDGLAQRAVRALKSRFPELGVMTDVALDPYTTHGQDGIIDDKGYVLNEITVEALVKQSLSHAHAGV
DIISPSDMMDGRIGAIRRALDADDHLNVRIMAYSAKYASAFYGPFRDAVGSAGNLGKADKTTYQMDPANGDEALREIALD
LEEGADMVMVKPGMPYLDVLRRVKEQFRVPTFAYQVSGEYAMLKAASANGWLDERKCVLEALMAFKRAGADGVLTYFAPQ
VARWLRETR

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=333, Percent_Identity=41.7417417417417, Blast_Score=238, Evalue=6e-63,
Organism=Escherichia coli, GI87081728, Length=318, Percent_Identity=44.6540880503145, Blast_Score=251, Evalue=7e-68,
Organism=Saccharomyces cerevisiae, GI6321398, Length=320, Percent_Identity=36.5625, Blast_Score=192, Evalue=6e-50,
Organism=Drosophila melanogaster, GI21358291, Length=311, Percent_Identity=40.1929260450161, Blast_Score=223, Evalue=2e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 36574; Mature: 36443

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHPHYRPRRMRHDAFSRRLMRENTLTTDDLIWPVFVHALSGRAPIASMPGVERLSLEEL
CCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
LREAENALELGIPVMDLFPVIDPAGKSLDAAEAWNPDGLAQRAVRALKSRFPELGVMTDV
HHHHHHHHHHCCCHHHHHHCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHC
ALDPYTTHGQDGIIDDKGYVLNEITVEALVKQSLSHAHAGVDIISPSDMMDGRIGAIRRA
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCHHHHCCHHHHHHHH
LDADDHLNVRIMAYSAKYASAFYGPFRDAVGSAGNLGKADKTTYQMDPANGDEALREIAL
HCCCCCCEEEEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHH
DLEEGADMVMVKPGMPYLDVLRRVKEQFRVPTFAYQVSGEYAMLKAASANGWLDERKCVL
HHHCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCEEEEEECCCCCCHHHHHHHH
EALMAFKRAGADGVLTYFAPQVARWLRETR
HHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AHPHYRPRRMRHDAFSRRLMRENTLTTDDLIWPVFVHALSGRAPIASMPGVERLSLEEL
CCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
LREAENALELGIPVMDLFPVIDPAGKSLDAAEAWNPDGLAQRAVRALKSRFPELGVMTDV
HHHHHHHHHHCCCHHHHHHCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHC
ALDPYTTHGQDGIIDDKGYVLNEITVEALVKQSLSHAHAGVDIISPSDMMDGRIGAIRRA
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCHHHHCCHHHHHHHH
LDADDHLNVRIMAYSAKYASAFYGPFRDAVGSAGNLGKADKTTYQMDPANGDEALREIAL
HCCCCCCEEEEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHH
DLEEGADMVMVKPGMPYLDVLRRVKEQFRVPTFAYQVSGEYAMLKAASANGWLDERKCVL
HHHCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCEEEEEECCCCCCHHHHHHHH
EALMAFKRAGADGVLTYFAPQVARWLRETR
HHHHHHHHCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9529530; 10984043; 10356331; 12079382 [H]