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The map label for this gene is leuB [H]

Identifier: 84489483

GI number: 84489483

Start: 784543

End: 785514

Strand: Direct

Name: leuB [H]

Synonym: Msp_0674

Alternate gene names: 84489483

Gene position: 784543-785514 (Clockwise)

Preceding gene: 84489473

Following gene: 84489484

Centisome position: 44.39

GC content: 29.42

Gene sequence:

>972_bases
TTGTATAAAATAACAGTAATACCTGGTGATGGTATAGGACAAGAAGTTATGCAACCAACAATAGATATTCTTGAAACATT
AAATTCTAAATTTGAATTTATACCAAAAGAAGCAGGAAAAGAATGTTATCAAAAATATGATACAAATCTACCTGAAGAGA
CTATTGTTCAATGTAGAGAAAGTGATAGTACACTCTTTGGAGCAGTAACATCAATTCCACAACAAAAAAGTGCAATAGTG
ACATTACGTAAAGAACTTGATTTATATGTAAATCAAAGACCAATACATTCATATACAAATCCAGATATAGATTTTACAAT
AATACGTGAAAATTCTGAAGGACTATATTCACATATTGAAGAAAGTACTGGTGATGAAGCAATAGCCATTAGAAAAATAA
CATACAAAGCTTCAGAAAGAATAATAAATTATGCTTTTAACTATGCACTTAAAACAGAAAAAAGTAAAGTAACAGCATCA
CATAAAGCAAATGTACTACCAGTAACTGATGGAATATTTAAAAATACATTCTATAAAGTAGCAAGTAATTATCCAACAAT
CAAATCCAATGATTATTATATAGATGCTATGGCAATGTATCTTATAACAAATCCTGCTCAATTTGATATTATAGTAACAA
CAAATCTCTTTGGAGACATATTATCTGATGAAGGTGGAGGATTAGTTGGAACTCTTGGACTTATACCTTCAGCAAATATT
GGGGATAAAACTGGATTATTTGAACCAGTACATGGATCAGCACCAGATATAGCAGGACTTAATAAGGCAAATCCAATAGC
AATGATCTTATCAAGTTGTTTAATGCTTGAATATTTAGGATTATATGATGATGCAAAAAGAATTCAAAATGCAGTAGAAG
AAACAATTTCTGAAAGTAAAGTTAAAACACCAGATATGGGTGGACATAACAATACACAAGATGTTGCAAATAATATACTC
CATAGATTATAA

Upstream 100 bases:

>100_bases
TACAATTGAAATATATAGTTTAAATAATATAAATAGCTATCAGTTACAAATTAAATCAACTTAAAAATAATTAACATAAA
ATAATAGGAGAATAAAAAAA

Downstream 100 bases:

>100_bases
TAAAATTTAAAAGATATGATAAATAAATTTATAAAGATAAAAATAGATAATAAAATTATATATTGAAAAAATATTTATTC
ATTAATTTATTTCATGTAAA

Product: isocitrate/isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; IMDH; Beta-IPM dehydrogenase [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MYKITVIPGDGIGQEVMQPTIDILETLNSKFEFIPKEAGKECYQKYDTNLPEETIVQCRESDSTLFGAVTSIPQQKSAIV
TLRKELDLYVNQRPIHSYTNPDIDFTIIRENSEGLYSHIEESTGDEAIAIRKITYKASERIINYAFNYALKTEKSKVTAS
HKANVLPVTDGIFKNTFYKVASNYPTIKSNDYYIDAMAMYLITNPAQFDIIVTTNLFGDILSDEGGGLVGTLGLIPSANI
GDKTGLFEPVHGSAPDIAGLNKANPIAMILSSCLMLEYLGLYDDAKRIQNAVEETISESKVKTPDMGGHNNTQDVANNIL
HRL

Sequences:

>Translated_323_residues
MYKITVIPGDGIGQEVMQPTIDILETLNSKFEFIPKEAGKECYQKYDTNLPEETIVQCRESDSTLFGAVTSIPQQKSAIV
TLRKELDLYVNQRPIHSYTNPDIDFTIIRENSEGLYSHIEESTGDEAIAIRKITYKASERIINYAFNYALKTEKSKVTAS
HKANVLPVTDGIFKNTFYKVASNYPTIKSNDYYIDAMAMYLITNPAQFDIIVTTNLFGDILSDEGGGLVGTLGLIPSANI
GDKTGLFEPVHGSAPDIAGLNKANPIAMILSSCLMLEYLGLYDDAKRIQNAVEETISESKVKTPDMGGHNNTQDVANNIL
HRL
>Mature_323_residues
MYKITVIPGDGIGQEVMQPTIDILETLNSKFEFIPKEAGKECYQKYDTNLPEETIVQCRESDSTLFGAVTSIPQQKSAIV
TLRKELDLYVNQRPIHSYTNPDIDFTIIRENSEGLYSHIEESTGDEAIAIRKITYKASERIINYAFNYALKTEKSKVTAS
HKANVLPVTDGIFKNTFYKVASNYPTIKSNDYYIDAMAMYLITNPAQFDIIVTTNLFGDILSDEGGGLVGTLGLIPSANI
GDKTGLFEPVHGSAPDIAGLNKANPIAMILSSCLMLEYLGLYDDAKRIQNAVEETISESKVKTPDMGGHNNTQDVANNIL
HRL

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=329, Percent_Identity=38.6018237082067, Blast_Score=226, Evalue=3e-59,
Organism=Homo sapiens, GI4758582, Length=339, Percent_Identity=34.8082595870207, Blast_Score=183, Evalue=2e-46,
Organism=Homo sapiens, GI28178821, Length=334, Percent_Identity=33.5329341317365, Blast_Score=176, Evalue=4e-44,
Organism=Homo sapiens, GI28178816, Length=334, Percent_Identity=33.2335329341317, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI28178838, Length=321, Percent_Identity=35.5140186915888, Blast_Score=172, Evalue=3e-43,
Organism=Homo sapiens, GI28178819, Length=197, Percent_Identity=39.0862944162437, Blast_Score=131, Evalue=8e-31,
Organism=Escherichia coli, GI1788101, Length=351, Percent_Identity=35.8974358974359, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI87081683, Length=356, Percent_Identity=34.8314606741573, Blast_Score=178, Evalue=5e-46,
Organism=Escherichia coli, GI1787381, Length=361, Percent_Identity=30.1939058171745, Blast_Score=152, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI71986051, Length=329, Percent_Identity=37.0820668693009, Blast_Score=218, Evalue=3e-57,
Organism=Caenorhabditis elegans, GI17550882, Length=340, Percent_Identity=33.5294117647059, Blast_Score=182, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI17505779, Length=334, Percent_Identity=33.5329341317365, Blast_Score=170, Evalue=9e-43,
Organism=Caenorhabditis elegans, GI25144293, Length=342, Percent_Identity=31.8713450292398, Blast_Score=164, Evalue=6e-41,
Organism=Saccharomyces cerevisiae, GI6322097, Length=346, Percent_Identity=42.485549132948, Blast_Score=249, Evalue=3e-67,
Organism=Saccharomyces cerevisiae, GI6324709, Length=334, Percent_Identity=38.0239520958084, Blast_Score=199, Evalue=4e-52,
Organism=Saccharomyces cerevisiae, GI6324291, Length=340, Percent_Identity=37.0588235294118, Blast_Score=199, Evalue=5e-52,
Organism=Saccharomyces cerevisiae, GI6319830, Length=352, Percent_Identity=34.375, Blast_Score=175, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24643270, Length=329, Percent_Identity=38.6018237082067, Blast_Score=223, Evalue=2e-58,
Organism=Drosophila melanogaster, GI24643268, Length=329, Percent_Identity=38.6018237082067, Blast_Score=223, Evalue=2e-58,
Organism=Drosophila melanogaster, GI24661184, Length=334, Percent_Identity=35.9281437125749, Blast_Score=208, Evalue=3e-54,
Organism=Drosophila melanogaster, GI161078637, Length=331, Percent_Identity=36.2537764350453, Blast_Score=194, Evalue=5e-50,
Organism=Drosophila melanogaster, GI161078635, Length=331, Percent_Identity=36.2537764350453, Blast_Score=194, Evalue=5e-50,
Organism=Drosophila melanogaster, GI161078633, Length=331, Percent_Identity=36.2537764350453, Blast_Score=194, Evalue=6e-50,
Organism=Drosophila melanogaster, GI24650122, Length=331, Percent_Identity=36.2537764350453, Blast_Score=194, Evalue=6e-50,
Organism=Drosophila melanogaster, GI161078639, Length=327, Percent_Identity=36.3914373088685, Blast_Score=191, Evalue=6e-49,
Organism=Drosophila melanogaster, GI281362242, Length=333, Percent_Identity=33.033033033033, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24648872, Length=333, Percent_Identity=33.033033033033, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI20130355, Length=328, Percent_Identity=25.9146341463415, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI45550436, Length=259, Percent_Identity=23.5521235521236, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011828 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 35750; Mature: 35750

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKITVIPGDGIGQEVMQPTIDILETLNSKFEFIPKEAGKECYQKYDTNLPEETIVQCRE
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHC
SDSTLFGAVTSIPQQKSAIVTLRKELDLYVNQRPIHSYTNPDIDFTIIRENSEGLYSHIE
CCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCHHHHHHH
ESTGDEAIAIRKITYKASERIINYAFNYALKTEKSKVTASHKANVLPVTDGIFKNTFYKV
HCCCCCEEEEEEEHHHHHHHHHHHHHHHHEECCCHHEECCCCCCEEECCCHHHHHHHHHH
ASNYPTIKSNDYYIDAMAMYLITNPAQFDIIVTTNLFGDILSDEGGGLVGTLGLIPSANI
HCCCCCCCCCCEEHHHEEEEHCCCCCEEEEEEECCHHHHHHCCCCCCEEEEECCCCCCCC
GDKTGLFEPVHGSAPDIAGLNKANPIAMILSSCLMLEYLGLYDDAKRIQNAVEETISESK
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHC
VKTPDMGGHNNTQDVANNILHRL
CCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MYKITVIPGDGIGQEVMQPTIDILETLNSKFEFIPKEAGKECYQKYDTNLPEETIVQCRE
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHC
SDSTLFGAVTSIPQQKSAIVTLRKELDLYVNQRPIHSYTNPDIDFTIIRENSEGLYSHIE
CCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCHHHHHHH
ESTGDEAIAIRKITYKASERIINYAFNYALKTEKSKVTASHKANVLPVTDGIFKNTFYKV
HCCCCCEEEEEEEHHHHHHHHHHHHHHHHEECCCHHEECCCCCCEEECCCHHHHHHHHHH
ASNYPTIKSNDYYIDAMAMYLITNPAQFDIIVTTNLFGDILSDEGGGLVGTLGLIPSANI
HCCCCCCCCCCEEHHHEEEEHCCCCCEEEEEEECCHHHHHHCCCCCCEEEEECCCCCCCC
GDKTGLFEPVHGSAPDIAGLNKANPIAMILSSCLMLEYLGLYDDAKRIQNAVEETISESK
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHC
VKTPDMGGHNNTQDVANNILHRL
CCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]