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The map label for this gene is pyrI

Identifier: 84489331

GI number: 84489331

Start: 617483

End: 617944

Strand: Direct

Name: pyrI

Synonym: Msp_0521

Alternate gene names: 84489331

Gene position: 617483-617944 (Clockwise)

Preceding gene: 84489330

Following gene: 84489332

Centisome position: 34.94

GC content: 27.71

Gene sequence:

>462_bases
ATGACAAAATCTGAATTAAAAGTTAAAAGAATTAAAAATGGAACAGTAATTGATCATATTACTGCTAACCGTTCATTAAA
TATTTTAAACATGCTTAAATTACCAGATGATGAAACAGCTATTATGGTTGCTATTAATGTTGAATCATCAGATATGGGAC
GTAAAGATATAATTAAAATTGAAGGAAGAGAATTATCACAAGATGAAGTTGATAAGTTAGTTCTTCTAGCACCTCAAGCA
ACACTCAATATTATTAGGGATTATCAAAATATTAGAAAATCCCACTTACATCTTATGGATGAAATTACTGATGTAGTCAC
ATGTAGTAATCCAAATTGTATAACAAATTCCAATGAACCAATACAAAAGAGATTTGCTGTACAAAATAAGCAACCTATTA
CACTTAGATGTTATTATTGTGAAAGAACTATGGAATATGATGATATAGAATCACAATTTTAG

Upstream 100 bases:

>100_bases
TACTCTCAAGTTATGAAATAAAAGACTTCAATACAGGTCAAGCTATTAAAACAAGAAAATGGAAAAAAGAAATAGAATAA
AAAAAAGGGAAGTAAATAAC

Downstream 100 bases:

>100_bases
AGTGATGTCATGTCAATAATACATAAAACAATAAGTATCAACACAAATAATGATATTGAATTAATAGATATTACAGAAGA
TATTCAAAGTATTCTTAGAA

Product: aspartate carbamoyltransferase regulatory subunit

Products: phosphate; N-carbamoyl-L-aspartate

Alternate protein names: NA

Number of amino acids: Translated: 153; Mature: 152

Protein sequence:

>153_residues
MTKSELKVKRIKNGTVIDHITANRSLNILNMLKLPDDETAIMVAINVESSDMGRKDIIKIEGRELSQDEVDKLVLLAPQA
TLNIIRDYQNIRKSHLHLMDEITDVVTCSNPNCITNSNEPIQKRFAVQNKQPITLRCYYCERTMEYDDIESQF

Sequences:

>Translated_153_residues
MTKSELKVKRIKNGTVIDHITANRSLNILNMLKLPDDETAIMVAINVESSDMGRKDIIKIEGRELSQDEVDKLVLLAPQA
TLNIIRDYQNIRKSHLHLMDEITDVVTCSNPNCITNSNEPIQKRFAVQNKQPITLRCYYCERTMEYDDIESQF
>Mature_152_residues
TKSELKVKRIKNGTVIDHITANRSLNILNMLKLPDDETAIMVAINVESSDMGRKDIIKIEGRELSQDEVDKLVLLAPQAT
LNIIRDYQNIRKSHLHLMDEITDVVTCSNPNCITNSNEPIQKRFAVQNKQPITLRCYYCERTMEYDDIESQF

Specific function: Involved in allosteric regulation of aspartate carbamoyltransferase

COG id: COG1781

COG function: function code F; Aspartate carbamoyltransferase, regulatory subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyrI family

Homologues:

Organism=Escherichia coli, GI1790692, Length=144, Percent_Identity=43.75, Blast_Score=125, Evalue=1e-30,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 440 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRI_METST (Q2NGY3)

Other databases:

- EMBL:   CP000102
- RefSeq:   YP_447563.1
- ProteinModelPortal:   Q2NGY3
- SMR:   Q2NGY3
- STRING:   Q2NGY3
- GeneID:   3856266
- GenomeReviews:   CP000102_GR
- KEGG:   mst:Msp_0521
- NMPDR:   fig|339860.6.peg.504
- eggNOG:   arNOG10217
- HOGENOM:   HBG305327
- OMA:   EGTVIDH
- PhylomeDB:   Q2NGY3
- ProtClustDB:   PRK00893
- BioCyc:   MSTA339860:MSP_0521-MONOMER
- HAMAP:   MF_00002
- InterPro:   IPR020545
- InterPro:   IPR002801
- InterPro:   IPR020542
- Gene3D:   G3DSA:2.30.30.20
- Gene3D:   G3DSA:3.30.70.140
- ProDom:   PD006194
- TIGRFAMs:   TIGR00240

Pfam domain/function: PF01948 PyrI; PF02748 PyrI_C; SSF54893 Asp_carbamoylTrfase_reg; SSF57825 Asp_transf_reg_C

EC number: 2.1.3.2

Molecular weight: Translated: 17625; Mature: 17493

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKSELKVKRIKNGTVIDHITANRSLNILNMLKLPDDETAIMVAINVESSDMGRKDIIKI
CCCCCCEEEECCCCCEEEEEECCCCCCEEEEEECCCCCCEEEEEEECCCCCCCCCCEEEE
EGRELSQDEVDKLVLLAPQATLNIIRDYQNIRKSHLHLMDEITDVVTCSNPNCITNSNEP
CCCCCCHHHHCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEECCCCCH
IQKRFAVQNKQPITLRCYYCERTMEYDDIESQF
HHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
TKSELKVKRIKNGTVIDHITANRSLNILNMLKLPDDETAIMVAINVESSDMGRKDIIKI
CCCCCEEEECCCCCEEEEEECCCCCCEEEEEECCCCCCEEEEEEECCCCCCCCCCEEEE
EGRELSQDEVDKLVLLAPQATLNIIRDYQNIRKSHLHLMDEITDVVTCSNPNCITNSNEP
CCCCCCHHHHCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEECCCCCH
IQKRFAVQNKQPITLRCYYCERTMEYDDIESQF
HHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: carbamoyl phosphate; L-aspartate

Specific reaction: carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA