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The map label for this gene is tgtA

Identifier: 84489330

GI number: 84489330

Start: 615438

End: 617462

Strand: Direct

Name: tgtA

Synonym: Msp_0520

Alternate gene names: 84489330

Gene position: 615438-617462 (Clockwise)

Preceding gene: 84489329

Following gene: 84489331

Centisome position: 34.82

GC content: 30.12

Gene sequence:

>2025_bases
ATGGTGGATATAATCGTGGATTTTGAAATTAAATATAAAGATGCAATGGCAAGGGTAGGTAAATTTAAAACACCACATGG
AACTGTAACCACTCCTGCTCTAATGCCTGTAGTTCATCCAGGTAAACAAACATTAGATGTTAAGAAGTTAGGTGCTCAGA
TAGTTATTACAAATTCATATATAATCTATAAAAATGAAGAACTTAAGAAAAAAGCATTAGAAGAAGGAGTTCATAGTCTA
ATAGATTTTCCTAATACAATAGAAACAGACTCTGGTTCATTTCAATTATCAGTATATGGTGATATAGATATTACAAATGA
AGAAGTAATAAAATTCCAAGAAGCAATAAAAACAGACATAGGAACATCACTAGATATTCCAACAGCACCATATGTAAAAA
GAGAAGAAGCAGAAAATGACCTTGAAATTACAATAGAACGTGCAAAGGAAGCAGCAAATGTAAAAAGTGATTTACTACTA
AATAGTGTTGTTCAAGGTTCCACATTTCCAGATTTAAGAGAAAAATGTGCAAAGGAAATATCTAAGTATGATGCAGATAT
TTATCCAATAGGAGCAGTAGTACCATTAATGGAAATGTATAGATATGCAGATTTAGTTGATGCTGTAATGTATTCAATGA
GAGGACTACCTGAAAACAAACCAAGACATCTTATGGGAGCAGGACATCCAATGGTATTTGCACTAGCAACAGCAATGGGT
TGTGATTTATTTGATAGTGCAGCATATATTTTATATGCAAATAAAGATAGATTCATGATGCCTGATGGTACACTAAGATT
AGAAGATCTTATAGAAATGCCATGTAGTTGTAGAGTATGTTGTGAATATACTGTAGATGAACTTAAACAAATGGACCAGA
AAAAACGTGCAAAACTCATAGCAGAACATAACCTTCATATTAGCTTTGCAGAAATTAGAAGAATAAGACAGGCAATTGTT
GATGGAGAATTAATGAAACTAGTAGAACTACGATGTAGATCACATCCATTTCTACTTGATGGACTAAGAAGACTCATGGA
ATATAAAGAAGATATGGAAAGATTAAATCCTAGTAGTAAAAAATCAGCATTCTTCTACACAGGATATGAATCTCTTGCAA
GAAGTGAAGTGCCTAAACACTTAAAACAATTAGAAAATATTAAGCCTAAGAATAAAAATCTAGTAATACTACCTCATACA
AGAAAACCATACACTAAATATGTAAATAGGGAATATATTAAGAAATACACACCAAAAATACCAACATATTACTCCAATAC
AACAAATACTGATTATTCAAACAGTGATGTTGTAGTAGCAGATATTCCATTTGGAATAATACCATTAGGATTAGATGAAT
TCTATCCATTAGCACAAAATGAATCCCCTTCAATTCATGATTTGGATTCAAAAAGATTTATCAGAGATATTATTAACAAC
TACTCTAAAAAATATGATAATGTTTTAATTCATAGGAAAGTTATTGAAAAATTTGACATCACCAACTTTAATCTTATAGA
AGATGAATTACAACTTCCTGAAGCTAAAATAAGTGATTTTAACCGTTTAAATGATATTGCTGATTACCAGTTTGGATGTG
GTGCTGGAAATGCATTATTTGGTGGAGATGAAGATAAAATCACCATAGAAAAAAGTCGTAAAACTAAAAAAATACGACAT
GTATTTGAAGATAATGAAAATATAGTAAATATGAGGGCAAATGATGGATTTTTAATTCTATCAGATCTTGGAGCAAAAAG
ATTACATAAATTCCTAGAATATCCACATAATAGGGTAGTTGTATCAGAAGATTCTGAACCATTTGCATTAAAGGGAAAAT
CAGTATTTAATAAGTTTGTACTAGATTGTGATGAAAATATAAGACGTAATGATGAAGTGTTAATTGTAAATAAGGATGAT
AAATTATTAGCATTTGGTAAGGCATTACTCTCAAGTTATGAAATAAAAGACTTCAATACAGGTCAAGCTATTAAAACAAG
AAAATGGAAAAAAGAAATAGAATAA

Upstream 100 bases:

>100_bases
TTGAATTTAATAATATTTCTTTTAATATAATTTTTTAATAAATTATTTCATTTAAGAAATATAAATATAATACATAATAT
AAGAAATAAAAAAAATAATA

Downstream 100 bases:

>100_bases
AAAAAAGGGAAGTAAATAACATGACAAAATCTGAATTAAAAGTTAAAAGAATTAAAAATGGAACAGTAATTGATCATATT
ACTGCTAACCGTTCATTAAA

Product: 7-cyano-7-deazaguanine tRNA-ribosyltransferase

Products: tRNAqueuine; guanine [C]

Alternate protein names: Archaeal tRNA-guanine transglycosylase

Number of amino acids: Translated: 674; Mature: 674

Protein sequence:

>674_residues
MVDIIVDFEIKYKDAMARVGKFKTPHGTVTTPALMPVVHPGKQTLDVKKLGAQIVITNSYIIYKNEELKKKALEEGVHSL
IDFPNTIETDSGSFQLSVYGDIDITNEEVIKFQEAIKTDIGTSLDIPTAPYVKREEAENDLEITIERAKEAANVKSDLLL
NSVVQGSTFPDLREKCAKEISKYDADIYPIGAVVPLMEMYRYADLVDAVMYSMRGLPENKPRHLMGAGHPMVFALATAMG
CDLFDSAAYILYANKDRFMMPDGTLRLEDLIEMPCSCRVCCEYTVDELKQMDQKKRAKLIAEHNLHISFAEIRRIRQAIV
DGELMKLVELRCRSHPFLLDGLRRLMEYKEDMERLNPSSKKSAFFYTGYESLARSEVPKHLKQLENIKPKNKNLVILPHT
RKPYTKYVNREYIKKYTPKIPTYYSNTTNTDYSNSDVVVADIPFGIIPLGLDEFYPLAQNESPSIHDLDSKRFIRDIINN
YSKKYDNVLIHRKVIEKFDITNFNLIEDELQLPEAKISDFNRLNDIADYQFGCGAGNALFGGDEDKITIEKSRKTKKIRH
VFEDNENIVNMRANDGFLILSDLGAKRLHKFLEYPHNRVVVSEDSEPFALKGKSVFNKFVLDCDENIRRNDEVLIVNKDD
KLLAFGKALLSSYEIKDFNTGQAIKTRKWKKEIE

Sequences:

>Translated_674_residues
MVDIIVDFEIKYKDAMARVGKFKTPHGTVTTPALMPVVHPGKQTLDVKKLGAQIVITNSYIIYKNEELKKKALEEGVHSL
IDFPNTIETDSGSFQLSVYGDIDITNEEVIKFQEAIKTDIGTSLDIPTAPYVKREEAENDLEITIERAKEAANVKSDLLL
NSVVQGSTFPDLREKCAKEISKYDADIYPIGAVVPLMEMYRYADLVDAVMYSMRGLPENKPRHLMGAGHPMVFALATAMG
CDLFDSAAYILYANKDRFMMPDGTLRLEDLIEMPCSCRVCCEYTVDELKQMDQKKRAKLIAEHNLHISFAEIRRIRQAIV
DGELMKLVELRCRSHPFLLDGLRRLMEYKEDMERLNPSSKKSAFFYTGYESLARSEVPKHLKQLENIKPKNKNLVILPHT
RKPYTKYVNREYIKKYTPKIPTYYSNTTNTDYSNSDVVVADIPFGIIPLGLDEFYPLAQNESPSIHDLDSKRFIRDIINN
YSKKYDNVLIHRKVIEKFDITNFNLIEDELQLPEAKISDFNRLNDIADYQFGCGAGNALFGGDEDKITIEKSRKTKKIRH
VFEDNENIVNMRANDGFLILSDLGAKRLHKFLEYPHNRVVVSEDSEPFALKGKSVFNKFVLDCDENIRRNDEVLIVNKDD
KLLAFGKALLSSYEIKDFNTGQAIKTRKWKKEIE
>Mature_674_residues
MVDIIVDFEIKYKDAMARVGKFKTPHGTVTTPALMPVVHPGKQTLDVKKLGAQIVITNSYIIYKNEELKKKALEEGVHSL
IDFPNTIETDSGSFQLSVYGDIDITNEEVIKFQEAIKTDIGTSLDIPTAPYVKREEAENDLEITIERAKEAANVKSDLLL
NSVVQGSTFPDLREKCAKEISKYDADIYPIGAVVPLMEMYRYADLVDAVMYSMRGLPENKPRHLMGAGHPMVFALATAMG
CDLFDSAAYILYANKDRFMMPDGTLRLEDLIEMPCSCRVCCEYTVDELKQMDQKKRAKLIAEHNLHISFAEIRRIRQAIV
DGELMKLVELRCRSHPFLLDGLRRLMEYKEDMERLNPSSKKSAFFYTGYESLARSEVPKHLKQLENIKPKNKNLVILPHT
RKPYTKYVNREYIKKYTPKIPTYYSNTTNTDYSNSDVVVADIPFGIIPLGLDEFYPLAQNESPSIHDLDSKRFIRDIINN
YSKKYDNVLIHRKVIEKFDITNFNLIEDELQLPEAKISDFNRLNDIADYQFGCGAGNALFGGDEDKITIEKSRKTKKIRH
VFEDNENIVNMRANDGFLILSDLGAKRLHKFLEYPHNRVVVSEDSEPFALKGKSVFNKFVLDCDENIRRNDEVLIVNKDD
KLLAFGKALLSSYEIKDFNTGQAIKTRKWKKEIE

Specific function: Exchanges the guanine residue with 7-cyano-7- deazaguanine (preQ0) at position 15 in the dihydrouridine loop (D- loop) of archaeal tRNAs

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI13654276, Length=344, Percent_Identity=26.7441860465116, Blast_Score=116, Evalue=6e-26,
Organism=Escherichia coli, GI1786607, Length=362, Percent_Identity=25.414364640884, Blast_Score=110, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17544680, Length=351, Percent_Identity=25.3561253561254, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI19920510, Length=350, Percent_Identity=26.8571428571429, Blast_Score=100, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ATGT_METST (Q2NGY4)

Other databases:

- EMBL:   CP000102
- RefSeq:   YP_447562.1
- ProteinModelPortal:   Q2NGY4
- STRING:   Q2NGY4
- GeneID:   3856265
- GenomeReviews:   CP000102_GR
- KEGG:   mst:Msp_0520
- NMPDR:   fig|339860.6.peg.503
- eggNOG:   arNOG04378
- HOGENOM:   HBG512800
- OMA:   EERCRAH
- ProtClustDB:   PRK13534
- BioCyc:   MSTA339860:MSP_0520-MONOMER
- HAMAP:   MF_01634
- InterPro:   IPR004804
- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR002616
- InterPro:   IPR004521
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- SMART:   SM00359
- TIGRFAMs:   TIGR00432
- TIGRFAMs:   TIGR00449
- TIGRFAMs:   TIGR00451

Pfam domain/function: PF01472 PUA; PF01702 TGT; SSF88697 PUA-like; SSF51713 tRNA_ribo_trans

EC number: 2.4.2.29 [C]

Molecular weight: Translated: 77222; Mature: 77222

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS50890 PUA

Important sites: ACT_SITE 90-90

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDIIVDFEIKYKDAMARVGKFKTPHGTVTTPALMPVVHPGKQTLDVKKLGAQIVITNSY
CEEEEEEEEEEHHHHHHHHCCCCCCCCCCCCCHHCCEECCCCHHHHHHHCCCEEEEECCE
IIYKNEELKKKALEEGVHSLIDFPNTIETDSGSFQLSVYGDIDITNEEVIKFQEAIKTDI
EEEECHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECEECCHHHHHHHHHHHHHHC
GTSLDIPTAPYVKREEAENDLEITIERAKEAANVKSDLLLNSVVQGSTFPDLREKCAKEI
CCCCCCCCCCCCCHHCCCCCCEEEEHHHHHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHH
SKYDADIYPIGAVVPLMEMYRYADLVDAVMYSMRGLPENKPRHLMGAGHPMVFALATAMG
HHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHC
CDLFDSAAYILYANKDRFMMPDGTLRLEDLIEMPCSCRVCCEYTVDELKQMDQKKRAKLI
CCHHCCCEEEEEECCCCEECCCCCEEHHHHHCCCCCCEEHHHCCHHHHHHHHHHHHHHHH
AEHNLHISFAEIRRIRQAIVDGELMKLVELRCRSHPFLLDGLRRLMEYKEDMERLNPSSK
HHCCCEEEHHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCC
KSAFFYTGYESLARSEVPKHLKQLENIKPKNKNLVILPHTRKPYTKYVNREYIKKYTPKI
CCEEEEECHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHCHHHHHHHCCCC
PTYYSNTTNTDYSNSDVVVADIPFGIIPLGLDEFYPLAQNESPSIHDLDSKRFIRDIINN
CCEECCCCCCCCCCCCEEEEECCCCEEECCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHH
YSKKYDNVLIHRKVIEKFDITNFNLIEDELQLPEAKISDFNRLNDIADYQFGCGAGNALF
HHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
GGDEDKITIEKSRKTKKIRHVFEDNENIVNMRANDGFLILSDLGAKRLHKFLEYPHNRVV
CCCCCCEEEEHHHHHHHHHHHHCCCCCEEEEECCCCEEEEECCCHHHHHHHHHCCCCEEE
VSEDSEPFALKGKSVFNKFVLDCDENIRRNDEVLIVNKDDKLLAFGKALLSSYEIKDFNT
EECCCCCCEECCHHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHHHCCCCCCCCCC
GQAIKTRKWKKEIE
CCCHHHHHHHHHCC
>Mature Secondary Structure
MVDIIVDFEIKYKDAMARVGKFKTPHGTVTTPALMPVVHPGKQTLDVKKLGAQIVITNSY
CEEEEEEEEEEHHHHHHHHCCCCCCCCCCCCCHHCCEECCCCHHHHHHHCCCEEEEECCE
IIYKNEELKKKALEEGVHSLIDFPNTIETDSGSFQLSVYGDIDITNEEVIKFQEAIKTDI
EEEECHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECEECCHHHHHHHHHHHHHHC
GTSLDIPTAPYVKREEAENDLEITIERAKEAANVKSDLLLNSVVQGSTFPDLREKCAKEI
CCCCCCCCCCCCCHHCCCCCCEEEEHHHHHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHH
SKYDADIYPIGAVVPLMEMYRYADLVDAVMYSMRGLPENKPRHLMGAGHPMVFALATAMG
HHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHC
CDLFDSAAYILYANKDRFMMPDGTLRLEDLIEMPCSCRVCCEYTVDELKQMDQKKRAKLI
CCHHCCCEEEEEECCCCEECCCCCEEHHHHHCCCCCCEEHHHCCHHHHHHHHHHHHHHHH
AEHNLHISFAEIRRIRQAIVDGELMKLVELRCRSHPFLLDGLRRLMEYKEDMERLNPSSK
HHCCCEEEHHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCC
KSAFFYTGYESLARSEVPKHLKQLENIKPKNKNLVILPHTRKPYTKYVNREYIKKYTPKI
CCEEEEECHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHCHHHHHHHCCCC
PTYYSNTTNTDYSNSDVVVADIPFGIIPLGLDEFYPLAQNESPSIHDLDSKRFIRDIINN
CCEECCCCCCCCCCCCEEEEECCCCEEECCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHH
YSKKYDNVLIHRKVIEKFDITNFNLIEDELQLPEAKISDFNRLNDIADYQFGCGAGNALF
HHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
GGDEDKITIEKSRKTKKIRHVFEDNENIVNMRANDGFLILSDLGAKRLHKFLEYPHNRVV
CCCCCCEEEEHHHHHHHHHHHHCCCCCEEEEECCCCEEEEECCCHHHHHHHHHCCCCEEE
VSEDSEPFALKGKSVFNKFVLDCDENIRRNDEVLIVNKDDKLLAFGKALLSSYEIKDFNT
EECCCCCCEECCHHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHHHCCCCCCCCCC
GQAIKTRKWKKEIE
CCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Zn; Mg [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.000014 {7-(aminomethyl)-7-deazaguanine}} 0.00006 {guanine}} 0.000053 {guanine}} [C]

Substrates: tRNAguanine; queuine [C]

Specific reaction: tRNAguanine + queuine = tRNAqueuine + guanine [C]

General reaction: Pentosyl group transfer [C]

Inhibitor: Bisimidoester; Dimethyl suberimidate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA