Click here to switch to the map view.

The map label for this gene is cofG

Identifier: 84489117

GI number: 84489117

Start: 357115

End: 358218

Strand: Direct

Name: cofG

Synonym: Msp_0296

Alternate gene names: 84489117

Gene position: 357115-358218 (Clockwise)

Preceding gene: 84489116

Following gene: 84489120

Centisome position: 20.21

GC content: 26.54

Gene sequence:

>1104_bases
GTGATATTTTTGAATCAATTATCAAAACAAGAAGCTAGAAATATTTTAGATTATGATATATTTGATATACTTAACATAAT
AAAAAAAATCAATATAAATAAGACAAATAAGAATATTACATATTCTAGAAATATTTTTCTACCTCTAACACACATCTGTC
AAAATAATTGTGGATACTGTACATTTAAACAAACACCACAAGAAGCAGATTTTTTATTAATGGATAAAGTGGAGGTACTT
TCAACAATTAAAAAATCTATGAAATATGGTTGTACAGAAGCATTATTCACATTTGGGGAATCAGCAGATAAAAATGAATT
AATTCAAGAAAAACTATCACGATATAATTATGAATCAATGGTAGATTACCTATATGATTTATCAAAAGAGGCTCTAAATA
ACTATAATATTCTACCACACACAAATATGGGCATAATATCAAGGGATGAGTTATATAAATTATCACAAGTAAATGCATCA
ATGGGGCTGATGCTTGAAACAACAAATGAAAAACTATTAAAAACAATAGTACATAGGGACAGTCCAGGTAAAAATCCAAA
GAAAAGAATAGAATTCATTGAAAATGCTGGAAAAGAAAACATTCCATTTACAACAGGATTACTAATAGGTATAGGTGAAT
CAAAAAATGATAGAATAGATTCTCTTCTTAAAATAAGAGAAATACAAGATCAATATGGACATATTCAGGAAATAATACTT
CAAAACTTCAAACCAAAAAGTGGAATACCTATGGAAAATCATCCAGAACCACCACTTGTTGATTTAATTAAATTAACATT
AGTTGCAACTTTGATGTTTCCAGATATTAGTATTCAAATACCACCAAATCTAAATCAGAAATTAATATCATTGTTTGCAT
TTATGGGAGCCGATGATGTTGGGGGAATATCACCTCTAACAATGGATTATGTTAATCCAGAGAGTAAATGGCCAAAAATT
AAAGAATTAGAATATGAACTAAATTCAATAAATTATAATTTAGTGGAAAGATTACCAGTATATGATAAGTACATTGATGA
AACTTATTTAAAAAAAGAAGTTTATGAAAAAGCATTGAAACTTCAAAAAAGAATTAAAAATTAA

Upstream 100 bases:

>100_bases
CTGGTTTTGGACCAATGTTTGTTGAAGCTATGGTTGTAAAATTACATTCATTAGATGAATAGTTTTCATCTTTCCTATTT
TTTTTATTCTATTTTTCGAG

Downstream 100 bases:

>100_bases
AGTACTCTAATATCAACAACTGTATGATGAACTCCTGGTGAATATTTTTTAATACTACGTTTGTTTAGAACTTCAATATC
ACGTCCCTGCTTTTTAGCAG

Product: FO synthase subunit 1

Products: NA

Alternate protein names: 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 1 [H]

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MIFLNQLSKQEARNILDYDIFDILNIIKKININKTNKNITYSRNIFLPLTHICQNNCGYCTFKQTPQEADFLLMDKVEVL
STIKKSMKYGCTEALFTFGESADKNELIQEKLSRYNYESMVDYLYDLSKEALNNYNILPHTNMGIISRDELYKLSQVNAS
MGLMLETTNEKLLKTIVHRDSPGKNPKKRIEFIENAGKENIPFTTGLLIGIGESKNDRIDSLLKIREIQDQYGHIQEIIL
QNFKPKSGIPMENHPEPPLVDLIKLTLVATLMFPDISIQIPPNLNQKLISLFAFMGADDVGGISPLTMDYVNPESKWPKI
KELEYELNSINYNLVERLPVYDKYIDETYLKKEVYEKALKLQKRIKN

Sequences:

>Translated_367_residues
MIFLNQLSKQEARNILDYDIFDILNIIKKININKTNKNITYSRNIFLPLTHICQNNCGYCTFKQTPQEADFLLMDKVEVL
STIKKSMKYGCTEALFTFGESADKNELIQEKLSRYNYESMVDYLYDLSKEALNNYNILPHTNMGIISRDELYKLSQVNAS
MGLMLETTNEKLLKTIVHRDSPGKNPKKRIEFIENAGKENIPFTTGLLIGIGESKNDRIDSLLKIREIQDQYGHIQEIIL
QNFKPKSGIPMENHPEPPLVDLIKLTLVATLMFPDISIQIPPNLNQKLISLFAFMGADDVGGISPLTMDYVNPESKWPKI
KELEYELNSINYNLVERLPVYDKYIDETYLKKEVYEKALKLQKRIKN
>Mature_367_residues
MIFLNQLSKQEARNILDYDIFDILNIIKKININKTNKNITYSRNIFLPLTHICQNNCGYCTFKQTPQEADFLLMDKVEVL
STIKKSMKYGCTEALFTFGESADKNELIQEKLSRYNYESMVDYLYDLSKEALNNYNILPHTNMGIISRDELYKLSQVNAS
MGLMLETTNEKLLKTIVHRDSPGKNPKKRIEFIENAGKENIPFTTGLLIGIGESKNDRIDSLLKIREIQDQYGHIQEIIL
QNFKPKSGIPMENHPEPPLVDLIKLTLVATLMFPDISIQIPPNLNQKLISLFAFMGADDVGGISPLTMDYVNPESKWPKI
KELEYELNSINYNLVERLPVYDKYIDETYLKKEVYEKALKLQKRIKN

Specific function: Catalyzes the radical-mediated transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino- 6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl- 8-hydroxy-5-deazariboflavin (FO) [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. CofG family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR006638
- InterPro:   IPR019939
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 42437; Mature: 42437

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFLNQLSKQEARNILDYDIFDILNIIKKININKTNKNITYSRNIFLPLTHICQNNCGYC
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCEEEEHHHHHCCCCCEE
TFKQTPQEADFLLMDKVEVLSTIKKSMKYGCTEALFTFGESADKNELIQEKLSRYNYESM
EECCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHCCHHHH
VDYLYDLSKEALNNYNILPHTNMGIISRDELYKLSQVNASMGLMLETTNEKLLKTIVHRD
HHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHCC
SPGKNPKKRIEFIENAGKENIPFTTGLLIGIGESKNDRIDSLLKIREIQDQYGHIQEIIL
CCCCCHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QNFKPKSGIPMENHPEPPLVDLIKLTLVATLMFPDISIQIPPNLNQKLISLFAFMGADDV
HCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHCCCCC
GGISPLTMDYVNPESKWPKIKELEYELNSINYNLVERLPVYDKYIDETYLKKEVYEKALK
CCCCCCEEECCCCCCCCCCHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
LQKRIKN
HHHHHCC
>Mature Secondary Structure
MIFLNQLSKQEARNILDYDIFDILNIIKKININKTNKNITYSRNIFLPLTHICQNNCGYC
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCEEEEHHHHHCCCCCEE
TFKQTPQEADFLLMDKVEVLSTIKKSMKYGCTEALFTFGESADKNELIQEKLSRYNYESM
EECCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHCCHHHH
VDYLYDLSKEALNNYNILPHTNMGIISRDELYKLSQVNASMGLMLETTNEKLLKTIVHRD
HHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHCC
SPGKNPKKRIEFIENAGKENIPFTTGLLIGIGESKNDRIDSLLKIREIQDQYGHIQEIIL
CCCCCHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QNFKPKSGIPMENHPEPPLVDLIKLTLVATLMFPDISIQIPPNLNQKLISLFAFMGADDV
HCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHCCCCC
GGISPLTMDYVNPESKWPKIKELEYELNSINYNLVERLPVYDKYIDETYLKKEVYEKALK
CCCCCCEEECCCCCCCCCCHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
LQKRIKN
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]