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The map label for this gene is trm1

Identifier: 84489112

GI number: 84489112

Start: 352446

End: 353618

Strand: Direct

Name: trm1

Synonym: Msp_0291

Alternate gene names: 84489112

Gene position: 352446-353618 (Clockwise)

Preceding gene: 84489109

Following gene: 84489114

Centisome position: 19.94

GC content: 31.2

Gene sequence:

>1173_bases
ATGGATACACATATTATTGAAGAAGGATTAGTTAAAATAGAAGTACCTGATTTTGAAAAAGTATCTGCAAAAGCACCTGT
TTTTTATAATCCAGTAATGGAAATGAATAGGGATATTTCTGTTGCAGTCATTAACCAGTATAGAAAAGAAGTAGATCATG
ATATTAGTATATGTGATGCTTTTGGTGGAACAGGTATTAGGGGAGCAAGATATTCTAAGGAAATACATGGTGTGGAAAAA
GTTGTTGTAGGTGATGTAAATCCATTAGCTGTTGAGATTTCTAAGAAAAATATGCAATTAAACAACATATCTAATGTTGA
AGTTCAAAAAAATGATGCAAACATTTTATTACAATCAAATAAGGGATTGTTCGATGTTGTGGATATAGATCCCTTTGGAA
CACCTGCAATGTTTACACAATCAACTGCAGCTAATATACGACCTGGTGGGTTAATTTGTATTAGTGCTACTGATACATCA
GCTTTATGTGGAACATACCATGATCCCTGTTTGAGAAAGTATGGTGCTAAACCTCTAAAAACAGAGTACTGTCATGAAAA
TGGTATTAGAATTTTAATAGCATTCATTTCAAGAAACTTAGCAGTTAACCAGAAATATTTACACGTACTATTTTCACATA
GTACAGAACATTACATGAGAGTATATGCTATTGTAAAACGTGGAGGTAAAAAAACTAACAAGGCATTGGATAATATTGGT
TTTATTGCACATTGTCCTAACTGTTTACATAGAGAAACTACGTATGGTTTTGCTCCTAAGATACCTCATACTTGTCCAGA
ATGTGGTGGGGAATATGATGTTGCAGGACCATTATGGTTAGGTAAAATATGGGATAAGGAATTTATTTATAATACTATGG
AACTAGTAAAAAACTTGAATTTAAATAAAAAAGATGATCTTATGTCCTTGTTTGAAAAGTGTTATATGGAAGCTGATGGT
CCTGTAACTTTCTATGATATTCATAAGATTTGTAAGAAATTAAAAATCAGTTCTCCTAAAATAAATGATGTTATGGATGA
AATTAGAAATAGAGGCTATTTTATTTCAAGGACTCATTTCAAGTTAACTGGTATGAGAACAGACATGCCACTTGATGAAC
TTAAACAATTAATTTTAGATATAAAAGAAGATAAACTTGGACCTGAAAAATAG

Upstream 100 bases:

>100_bases
ATTACCTAATTTTTTTTATACTCTTAATTTAATAAATAAAATAATAGAGATTTATAGAAAATATCTTTTTTTATAATAAA
TAATTATTAGGATGTAGATT

Downstream 100 bases:

>100_bases
GGAAAATTTTTTCCTTAACTTTTTTTTTAATTTTTATGAGAGTATACAACATTCTTTTTCAATATTTTAACATGATTTTA
TTGTTATTATGTGTATAATT

Product: N(2),N(2)-dimethylguanosine tRNA methyltransferase

Products: NA

Alternate protein names: tRNA 2,2-dimethylguanosine-26 methyltransferase; tRNA(guanine-26,N(2)-N(2)) methyltransferase; tRNA(m(2,2)G26)dimethyltransferase

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MDTHIIEEGLVKIEVPDFEKVSAKAPVFYNPVMEMNRDISVAVINQYRKEVDHDISICDAFGGTGIRGARYSKEIHGVEK
VVVGDVNPLAVEISKKNMQLNNISNVEVQKNDANILLQSNKGLFDVVDIDPFGTPAMFTQSTAANIRPGGLICISATDTS
ALCGTYHDPCLRKYGAKPLKTEYCHENGIRILIAFISRNLAVNQKYLHVLFSHSTEHYMRVYAIVKRGGKKTNKALDNIG
FIAHCPNCLHRETTYGFAPKIPHTCPECGGEYDVAGPLWLGKIWDKEFIYNTMELVKNLNLNKKDDLMSLFEKCYMEADG
PVTFYDIHKICKKLKISSPKINDVMDEIRNRGYFISRTHFKLTGMRTDMPLDELKQLILDIKEDKLGPEK

Sequences:

>Translated_390_residues
MDTHIIEEGLVKIEVPDFEKVSAKAPVFYNPVMEMNRDISVAVINQYRKEVDHDISICDAFGGTGIRGARYSKEIHGVEK
VVVGDVNPLAVEISKKNMQLNNISNVEVQKNDANILLQSNKGLFDVVDIDPFGTPAMFTQSTAANIRPGGLICISATDTS
ALCGTYHDPCLRKYGAKPLKTEYCHENGIRILIAFISRNLAVNQKYLHVLFSHSTEHYMRVYAIVKRGGKKTNKALDNIG
FIAHCPNCLHRETTYGFAPKIPHTCPECGGEYDVAGPLWLGKIWDKEFIYNTMELVKNLNLNKKDDLMSLFEKCYMEADG
PVTFYDIHKICKKLKISSPKINDVMDEIRNRGYFISRTHFKLTGMRTDMPLDELKQLILDIKEDKLGPEK
>Mature_390_residues
MDTHIIEEGLVKIEVPDFEKVSAKAPVFYNPVMEMNRDISVAVINQYRKEVDHDISICDAFGGTGIRGARYSKEIHGVEK
VVVGDVNPLAVEISKKNMQLNNISNVEVQKNDANILLQSNKGLFDVVDIDPFGTPAMFTQSTAANIRPGGLICISATDTS
ALCGTYHDPCLRKYGAKPLKTEYCHENGIRILIAFISRNLAVNQKYLHVLFSHSTEHYMRVYAIVKRGGKKTNKALDNIG
FIAHCPNCLHRETTYGFAPKIPHTCPECGGEYDVAGPLWLGKIWDKEFIYNTMELVKNLNLNKKDDLMSLFEKCYMEADG
PVTFYDIHKICKKLKISSPKINDVMDEIRNRGYFISRTHFKLTGMRTDMPLDELKQLILDIKEDKLGPEK

Specific function: Dimethylates a single guanine residue at position 26 of a number of tRNAs using S-adenosyl-L-methionine as donor of the methyl groups

COG id: COG1867

COG function: function code J; N2,N2-dimethylguanosine tRNA methyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TRM1 family

Homologues:

Organism=Homo sapiens, GI216548445, Length=329, Percent_Identity=25.531914893617, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI8923219, Length=345, Percent_Identity=24.0579710144928, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI209862871, Length=345, Percent_Identity=24.0579710144928, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI163792194, Length=471, Percent_Identity=25.2653927813163, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17564890, Length=420, Percent_Identity=26.6666666666667, Blast_Score=160, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6320325, Length=465, Percent_Identity=27.0967741935484, Blast_Score=157, Evalue=2e-39,
Organism=Drosophila melanogaster, GI19921190, Length=411, Percent_Identity=25.5474452554745, Blast_Score=157, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRM1_METST (Q2NI56)

Other databases:

- EMBL:   CP000102
- RefSeq:   YP_447344.1
- ProteinModelPortal:   Q2NI56
- SMR:   Q2NI56
- STRING:   Q2NI56
- GeneID:   3854938
- GenomeReviews:   CP000102_GR
- KEGG:   mst:Msp_0291
- NMPDR:   fig|339860.6.peg.282
- eggNOG:   arNOG04822
- HOGENOM:   HBG577872
- OMA:   DAPVFYN
- PhylomeDB:   Q2NI56
- ProtClustDB:   PRK04338
- BioCyc:   MSTA339860:MSP_0291-MONOMER
- HAMAP:   MF_00290
- InterPro:   IPR022923
- InterPro:   IPR002905
- PANTHER:   PTHR10631
- TIGRFAMs:   TIGR00308

Pfam domain/function: PF02005 TRM

EC number: =2.1.1.32

Molecular weight: Translated: 44038; Mature: 44038

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTHIIEEGLVKIEVPDFEKVSAKAPVFYNPVMEMNRDISVAVINQYRKEVDHDISICDA
CCCCCCCCCEEEEECCCCHHHCCCCCEEECCHHHCCCCCCHHHHHHHHHHHCCCCCHHHC
FGGTGIRGARYSKEIHGVEKVVVGDVNPLAVEISKKNMQLNNISNVEVQKNDANILLQSN
CCCCCCCCCHHHHHHHCCCEEEECCCCCEEEEEECCCEEECCCCCEEEEECCCEEEEECC
KGLFDVVDIDPFGTPAMFTQSTAANIRPGGLICISATDTSALCGTYHDPCLRKYGAKPLK
CCEEEEEECCCCCCCCCEECCCCCCCCCCCEEEEECCCCCHHHCCCCCHHHHHHCCCCCC
TEYCHENGIRILIAFISRNLAVNQKYLHVLFSHSTEHYMRVYAIVKRGGKKTNKALDNIG
HHHHHCCCCEEEEEEHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHCC
FIAHCPNCLHRETTYGFAPKIPHTCPECGGEYDVAGPLWLGKIWDKEFIYNTMELVKNLN
HHHCCCHHHHCCCCCCCCCCCCCCCHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCC
LNKKDDLMSLFEKCYMEADGPVTFYDIHKICKKLKISSPKINDVMDEIRNRGYFISRTHF
CCCHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEEEEEE
KLTGMRTDMPLDELKQLILDIKEDKLGPEK
EEECCCCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MDTHIIEEGLVKIEVPDFEKVSAKAPVFYNPVMEMNRDISVAVINQYRKEVDHDISICDA
CCCCCCCCCEEEEECCCCHHHCCCCCEEECCHHHCCCCCCHHHHHHHHHHHCCCCCHHHC
FGGTGIRGARYSKEIHGVEKVVVGDVNPLAVEISKKNMQLNNISNVEVQKNDANILLQSN
CCCCCCCCCHHHHHHHCCCEEEECCCCCEEEEEECCCEEECCCCCEEEEECCCEEEEECC
KGLFDVVDIDPFGTPAMFTQSTAANIRPGGLICISATDTSALCGTYHDPCLRKYGAKPLK
CCEEEEEECCCCCCCCCEECCCCCCCCCCCEEEEECCCCCHHHCCCCCHHHHHHCCCCCC
TEYCHENGIRILIAFISRNLAVNQKYLHVLFSHSTEHYMRVYAIVKRGGKKTNKALDNIG
HHHHHCCCCEEEEEEHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHCC
FIAHCPNCLHRETTYGFAPKIPHTCPECGGEYDVAGPLWLGKIWDKEFIYNTMELVKNLN
HHHCCCHHHHCCCCCCCCCCCCCCCHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCC
LNKKDDLMSLFEKCYMEADGPVTFYDIHKICKKLKISSPKINDVMDEIRNRGYFISRTHF
CCCHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEEEEEE
KLTGMRTDMPLDELKQLILDIKEDKLGPEK
EEECCCCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA