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Identifier: 84489085

GI number: 84489085

Start: 321097

End: 322770

Strand: Direct

Name: lig

Synonym: Msp_0258

Alternate gene names: 84489085

Gene position: 321097-322770 (Clockwise)

Preceding gene: 84489084

Following gene: 84489086

Centisome position: 18.17

GC content: 29.99

Gene sequence:

>1674_bases
ATGACAAATACTTCTTATGAAAAATTAGTTGAGGTATATGAAAAAATCAGTAGTACATCAAGTCGTCTAGAAAAACAAGA
TATTATAGCCGATTTTCTTATGTATATAAAAGAAACTGATGCTGATATAACATATGATATTACTCTATTACTTCAAGGTA
AAATATTTCCACCATGGAGTGATAAAGAAATGGGTATATCAACTCAACTAATAATAAAAGCACTATCCAATCTATTAGGT
GAAAGTACAAAATCCATAGAAAATAAGTTAGCAGAAGTAGGGGATATGGGGGAAATATCAGAGGAACTTATTAAGAATAA
CAAACAAGTAACATTCTTCAAAGTACCATTAACTGTTAAGAAAGTACTATCAAATCTTAGAAAAACAGAATCAATAACTG
GAAGTAAATCACAAAACAAAAAAATAAACTATCTTCTAGAATTATATACTTCAGCTTCACCTCTCGAGGCAAAATATATT
ACAAGAACAATAACAGAAAGATTAAGGATAGGTGTGGGTGAAGGAACACTAGTTGAGGCAATAGCTCAAGCATATAATAT
TGATAAAGAAATTATTGATAGAGCATATATGTTATCAAATGATCTTGGAGAAGTAGCATCTCGAGCACAAGAATCCATAG
AATCAGTAAAAAGCTTAACAATAACACCAGGAAAACCAATAAAACCAATGTTAGCACAATTAAGTCCAGGTATTAAAGAA
AGTATTACTGAGATGAAAAAGGTAATATGTGAAACAAAGTATGATGGAATAAGGGTTCAAATTCATCATTATAACAATAA
AACAAAGATATTTACAAGACGTTTAGAAAATGTAACAAATGCCCTACCTGAAGTAGTAGAATATATAGAAGAAGCCCTAC
CTTCTAAGGATTTTATTGTTGAAGGAGAAGTAATAGCAACAAAAGATAACAAACCAATATCATTCCAATACATACTTCAA
CGTGTAAAACGTAAATATGATATTGATAAAATGAGGGAGAAAATACCTTTAAAAGTATTTTTATTCGATGTATTATATTA
TGATAAGCCAACAGCAGAACTACCAATAATTCAAAGAAGACAATTACTTGAAGAGATTGTTACAACAAGTAAAAATGTGG
AACTAAGTACTATGAAAATAGTTTCTCAAGAAAATTATACAGAAGCAGAAAAATTATTCACATGGTCTATAGATGAAGGA
CATGAAGGTATAATGTTTAAAGATGTTACAAGTCCCTATTCTCCAGGTAAACGTGGAAAAAACATGCTTAAATACAAACC
ATTACGTGAAACACTTGATTGTGTAATAACAGGTGGAACTTATGGTAAAGGTAAAAGAGCTAAATTCTTTGGATCGTATT
TATTATCCTTACTTGATGAGAAAACTGGGGAATATAAAACATTAGTTCATGCAGCAACAGGTATGGATGATGATCTCTTA
GCTTCCCTTACAAAACGGATGGAAAAACTAATAATATCTAAATCAGAACAGAACGTTCTTTTCAAACCAAAAGTAATACT
TGAAATTGCATATAGTGAAATTGTAGAAAGTAATGAATATGAATCAGGTTATTCACTACGTTTTCCAGCAATAAAAGGAG
TTCGTGATGATATTGGTCTTGATGAGGTAGATACTTTAAGTAAACTTCATCAGATGATAGACTTACAAAAATAA

Upstream 100 bases:

>100_bases
TAAAACAAATCAAGGAAATAGTACAAAAAGAAGATATCATGATTTTTAAAATTAAACAATCAGAAGATAAACTATTTAAA
GCATTAATATAGGGATATAC

Downstream 100 bases:

>100_bases
TTATTAATAATACTTGTCATATTATTAATAAAAGTCGTTATGCATGATAAAGAAATATTTAAAATAACATTAACAACAAC
AATACTGGGACTTATAGGAT

Product: ATP-dependent DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [ATP]

Number of amino acids: Translated: 557; Mature: 556

Protein sequence:

>557_residues
MTNTSYEKLVEVYEKISSTSSRLEKQDIIADFLMYIKETDADITYDITLLLQGKIFPPWSDKEMGISTQLIIKALSNLLG
ESTKSIENKLAEVGDMGEISEELIKNNKQVTFFKVPLTVKKVLSNLRKTESITGSKSQNKKINYLLELYTSASPLEAKYI
TRTITERLRIGVGEGTLVEAIAQAYNIDKEIIDRAYMLSNDLGEVASRAQESIESVKSLTITPGKPIKPMLAQLSPGIKE
SITEMKKVICETKYDGIRVQIHHYNNKTKIFTRRLENVTNALPEVVEYIEEALPSKDFIVEGEVIATKDNKPISFQYILQ
RVKRKYDIDKMREKIPLKVFLFDVLYYDKPTAELPIIQRRQLLEEIVTTSKNVELSTMKIVSQENYTEAEKLFTWSIDEG
HEGIMFKDVTSPYSPGKRGKNMLKYKPLRETLDCVITGGTYGKGKRAKFFGSYLLSLLDEKTGEYKTLVHAATGMDDDLL
ASLTKRMEKLIISKSEQNVLFKPKVILEIAYSEIVESNEYESGYSLRFPAIKGVRDDIGLDEVDTLSKLHQMIDLQK

Sequences:

>Translated_557_residues
MTNTSYEKLVEVYEKISSTSSRLEKQDIIADFLMYIKETDADITYDITLLLQGKIFPPWSDKEMGISTQLIIKALSNLLG
ESTKSIENKLAEVGDMGEISEELIKNNKQVTFFKVPLTVKKVLSNLRKTESITGSKSQNKKINYLLELYTSASPLEAKYI
TRTITERLRIGVGEGTLVEAIAQAYNIDKEIIDRAYMLSNDLGEVASRAQESIESVKSLTITPGKPIKPMLAQLSPGIKE
SITEMKKVICETKYDGIRVQIHHYNNKTKIFTRRLENVTNALPEVVEYIEEALPSKDFIVEGEVIATKDNKPISFQYILQ
RVKRKYDIDKMREKIPLKVFLFDVLYYDKPTAELPIIQRRQLLEEIVTTSKNVELSTMKIVSQENYTEAEKLFTWSIDEG
HEGIMFKDVTSPYSPGKRGKNMLKYKPLRETLDCVITGGTYGKGKRAKFFGSYLLSLLDEKTGEYKTLVHAATGMDDDLL
ASLTKRMEKLIISKSEQNVLFKPKVILEIAYSEIVESNEYESGYSLRFPAIKGVRDDIGLDEVDTLSKLHQMIDLQK
>Mature_556_residues
TNTSYEKLVEVYEKISSTSSRLEKQDIIADFLMYIKETDADITYDITLLLQGKIFPPWSDKEMGISTQLIIKALSNLLGE
STKSIENKLAEVGDMGEISEELIKNNKQVTFFKVPLTVKKVLSNLRKTESITGSKSQNKKINYLLELYTSASPLEAKYIT
RTITERLRIGVGEGTLVEAIAQAYNIDKEIIDRAYMLSNDLGEVASRAQESIESVKSLTITPGKPIKPMLAQLSPGIKES
ITEMKKVICETKYDGIRVQIHHYNNKTKIFTRRLENVTNALPEVVEYIEEALPSKDFIVEGEVIATKDNKPISFQYILQR
VKRKYDIDKMREKIPLKVFLFDVLYYDKPTAELPIIQRRQLLEEIVTTSKNVELSTMKIVSQENYTEAEKLFTWSIDEGH
EGIMFKDVTSPYSPGKRGKNMLKYKPLRETLDCVITGGTYGKGKRAKFFGSYLLSLLDEKTGEYKTLVHAATGMDDDLLA
SLTKRMEKLIISKSEQNVLFKPKVILEIAYSEIVESNEYESGYSLRFPAIKGVRDDIGLDEVDTLSKLHQMIDLQK

Specific function: DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair

COG id: COG1793

COG function: function code L; ATP-dependent DNA ligase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent DNA ligase family

Homologues:

Organism=Homo sapiens, GI4557719, Length=610, Percent_Identity=26.2295081967213, Blast_Score=191, Evalue=2e-48,
Organism=Homo sapiens, GI73747829, Length=580, Percent_Identity=25.6896551724138, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI73747844, Length=580, Percent_Identity=25.6896551724138, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI46255052, Length=500, Percent_Identity=28, Blast_Score=135, Evalue=8e-32,
Organism=Homo sapiens, GI23199993, Length=500, Percent_Identity=28, Blast_Score=135, Evalue=8e-32,
Organism=Homo sapiens, GI148539894, Length=500, Percent_Identity=28, Blast_Score=135, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI71985269, Length=605, Percent_Identity=27.9338842975207, Blast_Score=201, Evalue=9e-52,
Organism=Caenorhabditis elegans, GI71985278, Length=192, Percent_Identity=25.5208333333333, Blast_Score=80, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6320038, Length=614, Percent_Identity=28.9902280130293, Blast_Score=230, Evalue=5e-61,
Organism=Drosophila melanogaster, GI24762424, Length=612, Percent_Identity=25, Blast_Score=179, Evalue=3e-45,
Organism=Drosophila melanogaster, GI85815841, Length=595, Percent_Identity=24.7058823529412, Blast_Score=117, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLI_METST (Q2NHF9)

Other databases:

- EMBL:   CP000102
- RefSeq:   YP_447317.1
- HSSP:   P71571
- ProteinModelPortal:   Q2NHF9
- SMR:   Q2NHF9
- STRING:   Q2NHF9
- GeneID:   3855692
- GenomeReviews:   CP000102_GR
- KEGG:   mst:Msp_0258
- NMPDR:   fig|339860.6.peg.255
- eggNOG:   arNOG04596
- HOGENOM:   HBG570821
- OMA:   GNWWKYK
- PhylomeDB:   Q2NHF9
- ProtClustDB:   CLSK798208
- BioCyc:   MSTA339860:MSP_0258-MONOMER
- HAMAP:   MF_00407
- InterPro:   IPR022865
- InterPro:   IPR000977
- InterPro:   IPR012309
- InterPro:   IPR012310
- InterPro:   IPR016059
- InterPro:   IPR012308
- InterPro:   IPR012340
- InterPro:   IPR016027
- Gene3D:   G3DSA:2.40.50.140
- TIGRFAMs:   TIGR00574

Pfam domain/function: PF04679 DNA_ligase_A_C; PF01068 DNA_ligase_A_M; PF04675 DNA_ligase_A_N; SSF50249 Nucleic_acid_OB

EC number: =6.5.1.1

Molecular weight: Translated: 63360; Mature: 63229

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00697 DNA_LIGASE_A1; PS00333 DNA_LIGASE_A2; PS50160 DNA_LIGASE_A3

Important sites: ACT_SITE 253-253 BINDING 251-251 BINDING 258-258 BINDING 273-273 BINDING 303-303 BINDING 342-342 BINDING 418-418 BINDING 424-424

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNTSYEKLVEVYEKISSTSSRLEKQDIIADFLMYIKETDADITYDITLLLQGKIFPPWS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCC
DKEMGISTQLIIKALSNLLGESTKSIENKLAEVGDMGEISEELIKNNKQVTFFKVPLTVK
CCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEECCHHHH
KVLSNLRKTESITGSKSQNKKINYLLELYTSASPLEAKYITRTITERLRIGVGEGTLVEA
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHH
IAQAYNIDKEIIDRAYMLSNDLGEVASRAQESIESVKSLTITPGKPIKPMLAQLSPGIKE
HHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHCCCHHH
SITEMKKVICETKYDGIRVQIHHYNNKTKIFTRRLENVTNALPEVVEYIEEALPSKDFIV
HHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
EGEVIATKDNKPISFQYILQRVKRKYDIDKMREKIPLKVFLFDVLYYDKPTAELPIIQRR
ECEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHH
QLLEEIVTTSKNVELSTMKIVSQENYTEAEKLFTWSIDEGHEGIMFKDVTSPYSPGKRGK
HHHHHHHHCCCCCEEHHHHHHCCCCCCCHHHEEEEECCCCCCCCEEEECCCCCCCCCCCC
NMLKYKPLRETLDCVITGGTYGKGKRAKFFGSYLLSLLDEKTGEYKTLVHAATGMDDDLL
CCHHCCHHHHHHCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHH
ASLTKRMEKLIISKSEQNVLFKPKVILEIAYSEIVESNEYESGYSLRFPAIKGVRDDIGL
HHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHCCCC
DEVDTLSKLHQMIDLQK
CHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TNTSYEKLVEVYEKISSTSSRLEKQDIIADFLMYIKETDADITYDITLLLQGKIFPPWS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCC
DKEMGISTQLIIKALSNLLGESTKSIENKLAEVGDMGEISEELIKNNKQVTFFKVPLTVK
CCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEECCHHHH
KVLSNLRKTESITGSKSQNKKINYLLELYTSASPLEAKYITRTITERLRIGVGEGTLVEA
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHH
IAQAYNIDKEIIDRAYMLSNDLGEVASRAQESIESVKSLTITPGKPIKPMLAQLSPGIKE
HHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHCCCHHH
SITEMKKVICETKYDGIRVQIHHYNNKTKIFTRRLENVTNALPEVVEYIEEALPSKDFIV
HHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
EGEVIATKDNKPISFQYILQRVKRKYDIDKMREKIPLKVFLFDVLYYDKPTAELPIIQRR
ECEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHH
QLLEEIVTTSKNVELSTMKIVSQENYTEAEKLFTWSIDEGHEGIMFKDVTSPYSPGKRGK
HHHHHHHHCCCCCEEHHHHHHCCCCCCCHHHEEEEECCCCCCCCEEEECCCCCCCCCCCC
NMLKYKPLRETLDCVITGGTYGKGKRAKFFGSYLLSLLDEKTGEYKTLVHAATGMDDDLL
CCHHCCHHHHHHCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHH
ASLTKRMEKLIISKSEQNVLFKPKVILEIAYSEIVESNEYESGYSLRFPAIKGVRDDIGL
HHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHCCCC
DEVDTLSKLHQMIDLQK
CHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA