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The map label for this gene is nadE

Identifier: 84488999

GI number: 84488999

Start: 211397

End: 212194

Strand: Direct

Name: nadE

Synonym: Msp_0170

Alternate gene names: 84488999

Gene position: 211397-212194 (Clockwise)

Preceding gene: 84488996

Following gene: 84489000

Centisome position: 11.96

GC content: 31.45

Gene sequence:

>798_bases
ATGATAGATTTACCTGAATTTGATGCAAGAAAATTTATAGAAAGAGCTTGTGAATTTATAAAAGAAAAAGTTGATGAATC
TAATTCTGATGGAGTAGTAATAGGATTAAGTGGTGGGATAGATTCATGTGTTGTAGCATGTCTGGCTGTTAGGGCATTAG
GTCCATTGCGTGTACGTGGATATATTCTTCCAACAATAACAACCTCAGATCAGGATTTATATGATGCAAAACTAATAAAG
GATGAACTGGATATTGAATCAGAATATATTTCAATAGGTAGTATCTATGATGAGTTTATAAGTTCATGTGAAATAAAAAA
TCTTCCACAAGATAATATTAATCTAGCACGTGGAAATCTAAAACCAAGAATTAGAATGAGTATTCTCTACTACTATGCAA
CAATATATAATTCACTTGTAATAGGAACAGGAAATAAAACAGAATTACAAGTAGGCTACTTTACAAAACATGGGGATGGT
GGAGTGGACTTATTACCAATAGGTGATTTATATAAGATGGATGTGAAAAAAGTTGCACAGGAATTAGGAGTACCATCACT
TATTATTAAGAAACCTCCAACAGCAGGTCTATGGGAGGGCCAAACTGATGAAGAGGAACTTGGCATGACATACAACATAC
TAGATAAATTATTATACTTATACCTTGAAGAAGAATATAGTATGCCAGATATAGCAAAAGAACTAGAAATTCCAGAAAGT
GAAGTAGAACGTATAATAAACATGGTAAATAATGCTAGTCATAAAAGAAATAAAATACCAATATTAAGTAAAAACTAA

Upstream 100 bases:

>100_bases
ATATATATGTAATTATAAATTCATTAATTATTATAAGTAAATAAAATAAATAATTATTCATTAAATGAATATTTATTAAT
TTATTTGATGGGATGTTTAT

Downstream 100 bases:

>100_bases
AATCTTAATTAATTTCTTTTTTCTTATTCTTTTATTTTTTTTTACAGTAAATTAAAATATTTAAATAAATATAAATATCT
ATTATTATAAAATTAACTAA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MIDLPEFDARKFIERACEFIKEKVDESNSDGVVIGLSGGIDSCVVACLAVRALGPLRVRGYILPTITTSDQDLYDAKLIK
DELDIESEYISIGSIYDEFISSCEIKNLPQDNINLARGNLKPRIRMSILYYYATIYNSLVIGTGNKTELQVGYFTKHGDG
GVDLLPIGDLYKMDVKKVAQELGVPSLIIKKPPTAGLWEGQTDEEELGMTYNILDKLLYLYLEEEYSMPDIAKELEIPES
EVERIINMVNNASHKRNKIPILSKN

Sequences:

>Translated_265_residues
MIDLPEFDARKFIERACEFIKEKVDESNSDGVVIGLSGGIDSCVVACLAVRALGPLRVRGYILPTITTSDQDLYDAKLIK
DELDIESEYISIGSIYDEFISSCEIKNLPQDNINLARGNLKPRIRMSILYYYATIYNSLVIGTGNKTELQVGYFTKHGDG
GVDLLPIGDLYKMDVKKVAQELGVPSLIIKKPPTAGLWEGQTDEEELGMTYNILDKLLYLYLEEEYSMPDIAKELEIPES
EVERIINMVNNASHKRNKIPILSKN
>Mature_265_residues
MIDLPEFDARKFIERACEFIKEKVDESNSDGVVIGLSGGIDSCVVACLAVRALGPLRVRGYILPTITTSDQDLYDAKLIK
DELDIESEYISIGSIYDEFISSCEIKNLPQDNINLARGNLKPRIRMSILYYYATIYNSLVIGTGNKTELQVGYFTKHGDG
GVDLLPIGDLYKMDVKKVAQELGVPSLIIKKPPTAGLWEGQTDEEELGMTYNILDKLLYLYLEEEYSMPDIAKELEIPES
EVERIINMVNNASHKRNKIPILSKN

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788036, Length=269, Percent_Identity=29.368029739777, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729 [H]

Pfam domain/function: PF02540 NAD_synthase [H]

EC number: =6.3.1.5 [H]

Molecular weight: Translated: 29750; Mature: 29750

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDLPEFDARKFIERACEFIKEKVDESNSDGVVIGLSGGIDSCVVACLAVRALGPLRVRG
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCEEEEE
YILPTITTSDQDLYDAKLIKDELDIESEYISIGSIYDEFISSCEIKNLPQDNINLARGNL
EEEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCC
KPRIRMSILYYYATIYNSLVIGTGNKTELQVGYFTKHGDGGVDLLPIGDLYKMDVKKVAQ
CCHHHHHHHHHHHHHHHCEEEECCCCCEEEEEEEEECCCCCEEEEECCHHHHHHHHHHHH
ELGVPSLIIKKPPTAGLWEGQTDEEELGMTYNILDKLLYLYLEEEYSMPDIAKELEIPES
HCCCCEEEEECCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHCCCCHH
EVERIINMVNNASHKRNKIPILSKN
HHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIDLPEFDARKFIERACEFIKEKVDESNSDGVVIGLSGGIDSCVVACLAVRALGPLRVRG
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCEEEEE
YILPTITTSDQDLYDAKLIKDELDIESEYISIGSIYDEFISSCEIKNLPQDNINLARGNL
EEEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCC
KPRIRMSILYYYATIYNSLVIGTGNKTELQVGYFTKHGDGGVDLLPIGDLYKMDVKKVAQ
CCHHHHHHHHHHHHHHHCEEEECCCCCEEEEEEEEECCCCCEEEEECCHHHHHHHHHHHH
ELGVPSLIIKKPPTAGLWEGQTDEEELGMTYNILDKLLYLYLEEEYSMPDIAKELEIPES
HCCCCEEEEECCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHCCCCHH
EVERIINMVNNASHKRNKIPILSKN
HHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]