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The map label for this gene is otsA [H]

Identifier: 83721649

GI number: 83721649

Start: 1960146

End: 1961522

Strand: Direct

Name: otsA [H]

Synonym: BTH_I1753

Alternate gene names: 83721649

Gene position: 1960146-1961522 (Clockwise)

Preceding gene: 83720965

Following gene: 83718898

Centisome position: 51.46

GC content: 68.7

Gene sequence:

>1377_bases
ATGAGCCGTCTCATCATCGTCTCGAATCGGGTCGCGCCGATTTCGGAAGGGGAGCCCGCCGCGGGCGGTCTCGCGATCGG
TGTCTACGACGCGCTGAAGGAAACGGGCGGCACGTGGTTCGGCTGGAGCGGCGAGGTTGTCGCGTCGGGCGCGCCGCAGA
TTCGCGTCGAGGAGCACGGCCCCGTCACGTTCGCGACGATCGGGCTGTCGCGCCGCGATTACGATCAGTACTACCGAGGC
TTCTCGAACGCGACGCTGTGGCCGGCGTTCCATTACCGCGCGGACCTGATTCAGTTCGATCGCCACGAGTTCGAGGGCTA
TTCGCGCGTGAACGTGTGGCTCGCGCGGCAGCTCGTGCCGCTGTTGCGCGACGACGACGTGATCTGGGTGCACGACTATC
ACCTGATTCCGTTCGCGCAGGCGCTGCGCGCGGCCGGCGTGAAGAACCGGATCGGCTTCTTCCTGCACATTCCGTTTCCG
GCCGCGCAGGTGCTCGTCAACGTGCCGCCGCATCGGGAGCTTGTCGAGGCGCTGTGCTCGTTCGATCTGCTCGGCTTCCA
GACCGACACCGATCTGCGCGCGTTCTTCGAATACGTCGAATGCGAGGCGGGCGGCGCGGTCGACCGCGCCGCGCACCCGG
CGCGCGTCGAGGCGTTCGGGCGCACGCTGCGCGCGGCCGCGTATCCGATCGGCGTCTATCCGGACGAGATCGCCGCGCTC
GCGAAGGCGGGCGAGAACGGCCGCGCGGTGAAGGCGCTCGCGGCGTCGCTGCGCGGGCGGCAGCTCGTGATGAGCGTCGA
CCGGCTCGATTACTCGAAGGGGCTCGTCGAGCGCTTTCGCGCGTTCGAGAAGCTGCTCGAGCACGAGACGTCGTTTCGCA
ATCACGTGTCGTTCCTGCAGATCGCGCCGTCGACGCGCGCCGACCTGCGCGCGTACCAGGACATCCGCCGTCAGCTCGAA
GGCGAGTCGGGGCGCATCAATGGGCGCTTCGCGGAGCTCGACTGGGCGCCGATCCTCTACATCCATCGGCAGTACGAGCG
GCCGGTGCTGGCCGCGCTGTACCGGCTCGCGCGTGTCGGCTACGTGACGCCGCTGCGCGACGGGATGAATCTCGTCGCGA
AGGAGTATGTGTCCGCGCAGGACCCGGACGATCCCGGCGTGCTGGTGCTGTCGCGCTTCGCGGGCGCGGCGTGCGAGCTT
ACGGGCGCGCTGATCGTCAATCCGATCGACATCGCCGGGATGGCCGACGCGTTGTCGCGCGCGCTGTCGATGCCGCTCGC
CGAGCGGCGCGCGCGTCACGCCGACATGCTCGCGCGGCTGCGCGAGAACAACGTGTCGGTGTGGCGCGACAACTTCTTGC
GGGATCTCGGGCGATAG

Upstream 100 bases:

>100_bases
AAGTCGGCGCGGGCGATACGGCGGCGCATGTGCGCGTCGATTCGGTCGCCGCGTTCCGCGAGCAGCTCGCGCGCTGGATC
GACGCGGGGCGCGCGCTCGC

Downstream 100 bases:

>100_bases
CGCGCGGCGGCGGGCGAGGGCTGCCTGCTCTCGCCGCTCCGGGGCGGCGCGATCGCGCGGGCGTGAGGGGCGATGGTTCG
GACGTCCGCACGCCGCATCC

Product: alpha,alpha-trehalose-phosphate synthase

Products: NA

Alternate protein names: Osmoregulatory trehalose synthesis protein A; Trehalose-6-phosphate synthase; UDP-glucose-glucosephosphate glucosyltransferase [H]

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MSRLIIVSNRVAPISEGEPAAGGLAIGVYDALKETGGTWFGWSGEVVASGAPQIRVEEHGPVTFATIGLSRRDYDQYYRG
FSNATLWPAFHYRADLIQFDRHEFEGYSRVNVWLARQLVPLLRDDDVIWVHDYHLIPFAQALRAAGVKNRIGFFLHIPFP
AAQVLVNVPPHRELVEALCSFDLLGFQTDTDLRAFFEYVECEAGGAVDRAAHPARVEAFGRTLRAAAYPIGVYPDEIAAL
AKAGENGRAVKALAASLRGRQLVMSVDRLDYSKGLVERFRAFEKLLEHETSFRNHVSFLQIAPSTRADLRAYQDIRRQLE
GESGRINGRFAELDWAPILYIHRQYERPVLAALYRLARVGYVTPLRDGMNLVAKEYVSAQDPDDPGVLVLSRFAGAACEL
TGALIVNPIDIAGMADALSRALSMPLAERRARHADMLARLRENNVSVWRDNFLRDLGR

Sequences:

>Translated_458_residues
MSRLIIVSNRVAPISEGEPAAGGLAIGVYDALKETGGTWFGWSGEVVASGAPQIRVEEHGPVTFATIGLSRRDYDQYYRG
FSNATLWPAFHYRADLIQFDRHEFEGYSRVNVWLARQLVPLLRDDDVIWVHDYHLIPFAQALRAAGVKNRIGFFLHIPFP
AAQVLVNVPPHRELVEALCSFDLLGFQTDTDLRAFFEYVECEAGGAVDRAAHPARVEAFGRTLRAAAYPIGVYPDEIAAL
AKAGENGRAVKALAASLRGRQLVMSVDRLDYSKGLVERFRAFEKLLEHETSFRNHVSFLQIAPSTRADLRAYQDIRRQLE
GESGRINGRFAELDWAPILYIHRQYERPVLAALYRLARVGYVTPLRDGMNLVAKEYVSAQDPDDPGVLVLSRFAGAACEL
TGALIVNPIDIAGMADALSRALSMPLAERRARHADMLARLRENNVSVWRDNFLRDLGR
>Mature_457_residues
SRLIIVSNRVAPISEGEPAAGGLAIGVYDALKETGGTWFGWSGEVVASGAPQIRVEEHGPVTFATIGLSRRDYDQYYRGF
SNATLWPAFHYRADLIQFDRHEFEGYSRVNVWLARQLVPLLRDDDVIWVHDYHLIPFAQALRAAGVKNRIGFFLHIPFPA
AQVLVNVPPHRELVEALCSFDLLGFQTDTDLRAFFEYVECEAGGAVDRAAHPARVEAFGRTLRAAAYPIGVYPDEIAALA
KAGENGRAVKALAASLRGRQLVMSVDRLDYSKGLVERFRAFEKLLEHETSFRNHVSFLQIAPSTRADLRAYQDIRRQLEG
ESGRINGRFAELDWAPILYIHRQYERPVLAALYRLARVGYVTPLRDGMNLVAKEYVSAQDPDDPGVLVLSRFAGAACELT
GALIVNPIDIAGMADALSRALSMPLAERRARHADMLARLRENNVSVWRDNFLRDLGR

Specific function: Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=458, Percent_Identity=52.8384279475983, Blast_Score=474, Evalue=1e-135,
Organism=Caenorhabditis elegans, GI115533558, Length=421, Percent_Identity=28.978622327791, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI115533560, Length=423, Percent_Identity=28.8416075650118, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI71987012, Length=414, Percent_Identity=28.5024154589372, Blast_Score=141, Evalue=6e-34,
Organism=Saccharomyces cerevisiae, GI6319602, Length=470, Percent_Identity=31.9148936170213, Blast_Score=267, Evalue=3e-72,
Organism=Saccharomyces cerevisiae, GI6323537, Length=435, Percent_Identity=26.6666666666667, Blast_Score=167, Evalue=4e-42,
Organism=Saccharomyces cerevisiae, GI6320279, Length=396, Percent_Identity=24.7474747474747, Blast_Score=150, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6323917, Length=378, Percent_Identity=27.2486772486773, Blast_Score=147, Evalue=2e-36,
Organism=Drosophila melanogaster, GI19920676, Length=489, Percent_Identity=35.9918200408998, Blast_Score=272, Evalue=3e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830
- InterPro:   IPR012766 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: =2.4.1.15 [H]

Molecular weight: Translated: 51174; Mature: 51043

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLIIVSNRVAPISEGEPAAGGLAIGVYDALKETGGTWFGWSGEVVASGAPQIRVEEHG
CCCEEEEECCCCCCCCCCCCCCCEEEHHHHHHHHCCCCEECCCCCEEECCCCEEEEECCC
PVTFATIGLSRRDYDQYYRGFSNATLWPAFHYRADLIQFDRHEFEGYSRVNVWLARQLVP
CEEEEEECCCCCCHHHHHCCCCCCEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LLRDDDVIWVHDYHLIPFAQALRAAGVKNRIGFFLHIPFPAAQVLVNVPPHRELVEALCS
HHCCCCEEEEECCCHHHHHHHHHHCCCCCCCCEEEECCCCHHHHEEECCCHHHHHHHHHC
FDLLGFQTDTDLRAFFEYVECEAGGAVDRAAHPARVEAFGRTLRAAAYPIGVYPDEIAAL
CHHCCCCCCHHHHHHHHHHHHCCCCCCHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHH
AKAGENGRAVKALAASLRGRQLVMSVDRLDYSKGLVERFRAFEKLLEHETSFRNHVSFLQ
HHCCCCCHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
IAPSTRADLRAYQDIRRQLEGESGRINGRFAELDWAPILYIHRQYERPVLAALYRLARVG
ECCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCEEEEEHHHHCCHHHHHHHHHHHHC
YVTPLRDGMNLVAKEYVSAQDPDDPGVLVLSRFAGAACELTGALIVNPIDIAGMADALSR
CCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEHHCCCHHEECCEEEECCHHHHHHHHHHHH
ALSMPLAERRARHADMLARLRENNVSVWRDNFLRDLGR
HHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SRLIIVSNRVAPISEGEPAAGGLAIGVYDALKETGGTWFGWSGEVVASGAPQIRVEEHG
CCEEEEECCCCCCCCCCCCCCCEEEHHHHHHHHCCCCEECCCCCEEECCCCEEEEECCC
PVTFATIGLSRRDYDQYYRGFSNATLWPAFHYRADLIQFDRHEFEGYSRVNVWLARQLVP
CEEEEEECCCCCCHHHHHCCCCCCEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LLRDDDVIWVHDYHLIPFAQALRAAGVKNRIGFFLHIPFPAAQVLVNVPPHRELVEALCS
HHCCCCEEEEECCCHHHHHHHHHHCCCCCCCCEEEECCCCHHHHEEECCCHHHHHHHHHC
FDLLGFQTDTDLRAFFEYVECEAGGAVDRAAHPARVEAFGRTLRAAAYPIGVYPDEIAAL
CHHCCCCCCHHHHHHHHHHHHCCCCCCHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHH
AKAGENGRAVKALAASLRGRQLVMSVDRLDYSKGLVERFRAFEKLLEHETSFRNHVSFLQ
HHCCCCCHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
IAPSTRADLRAYQDIRRQLEGESGRINGRFAELDWAPILYIHRQYERPVLAALYRLARVG
ECCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCEEEEEHHHHCCHHHHHHHHHHHHC
YVTPLRDGMNLVAKEYVSAQDPDDPGVLVLSRFAGAACELTGALIVNPIDIAGMADALSR
CCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEHHCCCHHEECCEEEECCHHHHHHHHHHHH
ALSMPLAERRARHADMLARLRENNVSVWRDNFLRDLGR
HHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA