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The map label for this gene is ilvB [H]

Identifier: 83719261

GI number: 83719261

Start: 1186036

End: 1187799

Strand: Direct

Name: ilvB [H]

Synonym: BTH_I1045

Alternate gene names: 83719261

Gene position: 1186036-1187799 (Clockwise)

Preceding gene: 83721269

Following gene: 83718553

Centisome position: 31.14

GC content: 63.32

Gene sequence:

>1764_bases
ATGAACATGCCCAGCGCGGAATTCTCCACGTCGGAATCCCTTTCCCCTCAGAAAACCGGCTCCATCGGCGCCACCGTGCT
CATGAAGGCACTGGCCGACGAGAACGTCGAATTCATCTGGGGTTACCCTGGCGGCTCGGTCCTCTACATCTACGACGAGC
TGTACAAGCAAGACAAGATTCAACACGTGCTCGTGCGCCACGAGCAGGCAGCCGTGCACGCCGCGGACGCGTACTCGCGC
TCGACGGGCAAGGTCGGCGTGTGCCTCGTCACGTCCGGCCCGGGCGTGACGAACGCGGTGACGGGCATCGCCACGGCCTA
CATGGATTCGATCCCGCTCGTCGTGATCAGCGGCCAGGTGCCGACCGCGGCGATCGGCCAGGACGCGTTCCAGGAGTGCG
ACACCGTCGGCATCACGCGGCCGTGCGTGAAGCACAACTTCCTCGTGAAGGACGTGCGCGAGCTCGCGGAAACCGTCAAG
AAGGCGTTCTACATCGCGCGCACCGGCCGGCCCGGCCCGGTCCTCATCGACATCCCGAAGGACATCTCGAAGACGCCGTG
CGAGTACGAACCCGTCAAGAACGTGTCGCTGCGCTCGTACAACCCGGTCACGAAGGGCCACTCGGGGCAGATCCGCAAGG
CGGTGTCGCTCCTCTTGTCCGCGAAGCGCCCGTACATCTACACGGGCGGCGGCATCATCCTCGCCGATGCGTCGCGCGAG
CTGAACCAGTTCGCCGATCTGCTCGGCTATCCCGTCACGAACACGCTGATGGGCCTCGGCGGCTATCGCGCGTCGGACCG
GAAATTCCTCGGCATGCTCGGCATGCACGGCACGTACGAAGCGAACATGGCGATGCAGCACTGCGACGTGCTGATCGCGA
TCGGCGCGCGCTTCGACGACCGCGTGATCGGCGATCCGGCGCACTTCGCTTCGCGTCCGCGCAAGATCATCCACATCGAC
ATCGATCCGTCGTCGATCAGCAAGCGCGTGAAGGTCGACATCCCGATCGTCGGCGACGTGAAGGAAGTGCTGAAGGATCT
GATCGAGCAGTTGCAGACGGCCGAGCACGGCCCCGACGCCGACGCGCTCGCGCAATGGTGGAAGGACATCGAGGGCTGGC
GCGCGAAGGACTGCCTGAAGTACGACCGCGAAAGCGAGATCATCAAGCCGCAGTACGTGGTCGAGAAGGCGTGGGAGCTG
ACAGACGGCAATGCGTTCGTGTGCTCGGACGTCGGGCAGCACCAGATGTGGGCCGCGCAGTTCTATCGTTTCAACAAGCC
GCGTCGCTGGATCAATTCCGGCGGCCTCGGCACGATGGGCTTCGGCCTGCCGGCAGCGATGGGCGTCAAGATGGCCCATC
CGGACGACGACGTGCTGTGCATCACGGGCGAGGGCTCGATCCAGATGTGCATCCAGGAGCTGTCGACCTGCAAGCAGTAC
GACACTCCGGTGAAGATCATTTCGCTGAACAACCGCTATCTCGGCATGGTCCGCCAGTGGCAGCAGATCGAATACAGCAA
GCGCTATTCGCATTCGTACATGGATGCGCTGCCGGATTTCGTGAAGCTCGCGCAGGCGTACGGCCATATCGGGATGCGCA
TCGAAAAATCCGCGGACGTCGAGCCTGCGCTCAAGGAAGCGCTGCGCCTGAAGGACCGCACCGTGTTTCTCGACTTCCAG
ACCGACCCGACCGAAAACGTCTGGCCAATGGTACAGGCCGGAAAGGGCATCACCGAGATGCTGCTCGGATCGGAAGATCT
GTAA

Upstream 100 bases:

>100_bases
TGAAGCCCGCCTTTCAACTGGACGCGCCGCTTGAACCCGAGCCACAAGCCCGGCAGAGAGCACCCGATCAATTTTTTGCC
GAAAATTCGAAAGGTCAAAG

Downstream 100 bases:

>100_bases
CGGCGCGATGCGCTCGAGGCCGCGGGCGGCCGCCTTCACGAAGGGCGGCGCGGCACGCGGGGAGCGCCGCGCGATACGCA
ACGACACGGGATTATTCTGG

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS-III; ALS-III; Acetohydroxy-acid synthase III large subunit [H]

Number of amino acids: Translated: 587; Mature: 587

Protein sequence:

>587_residues
MNMPSAEFSTSESLSPQKTGSIGATVLMKALADENVEFIWGYPGGSVLYIYDELYKQDKIQHVLVRHEQAAVHAADAYSR
STGKVGVCLVTSGPGVTNAVTGIATAYMDSIPLVVISGQVPTAAIGQDAFQECDTVGITRPCVKHNFLVKDVRELAETVK
KAFYIARTGRPGPVLIDIPKDISKTPCEYEPVKNVSLRSYNPVTKGHSGQIRKAVSLLLSAKRPYIYTGGGIILADASRE
LNQFADLLGYPVTNTLMGLGGYRASDRKFLGMLGMHGTYEANMAMQHCDVLIAIGARFDDRVIGDPAHFASRPRKIIHID
IDPSSISKRVKVDIPIVGDVKEVLKDLIEQLQTAEHGPDADALAQWWKDIEGWRAKDCLKYDRESEIIKPQYVVEKAWEL
TDGNAFVCSDVGQHQMWAAQFYRFNKPRRWINSGGLGTMGFGLPAAMGVKMAHPDDDVLCITGEGSIQMCIQELSTCKQY
DTPVKIISLNNRYLGMVRQWQQIEYSKRYSHSYMDALPDFVKLAQAYGHIGMRIEKSADVEPALKEALRLKDRTVFLDFQ
TDPTENVWPMVQAGKGITEMLLGSEDL

Sequences:

>Translated_587_residues
MNMPSAEFSTSESLSPQKTGSIGATVLMKALADENVEFIWGYPGGSVLYIYDELYKQDKIQHVLVRHEQAAVHAADAYSR
STGKVGVCLVTSGPGVTNAVTGIATAYMDSIPLVVISGQVPTAAIGQDAFQECDTVGITRPCVKHNFLVKDVRELAETVK
KAFYIARTGRPGPVLIDIPKDISKTPCEYEPVKNVSLRSYNPVTKGHSGQIRKAVSLLLSAKRPYIYTGGGIILADASRE
LNQFADLLGYPVTNTLMGLGGYRASDRKFLGMLGMHGTYEANMAMQHCDVLIAIGARFDDRVIGDPAHFASRPRKIIHID
IDPSSISKRVKVDIPIVGDVKEVLKDLIEQLQTAEHGPDADALAQWWKDIEGWRAKDCLKYDRESEIIKPQYVVEKAWEL
TDGNAFVCSDVGQHQMWAAQFYRFNKPRRWINSGGLGTMGFGLPAAMGVKMAHPDDDVLCITGEGSIQMCIQELSTCKQY
DTPVKIISLNNRYLGMVRQWQQIEYSKRYSHSYMDALPDFVKLAQAYGHIGMRIEKSADVEPALKEALRLKDRTVFLDFQ
TDPTENVWPMVQAGKGITEMLLGSEDL
>Mature_587_residues
MNMPSAEFSTSESLSPQKTGSIGATVLMKALADENVEFIWGYPGGSVLYIYDELYKQDKIQHVLVRHEQAAVHAADAYSR
STGKVGVCLVTSGPGVTNAVTGIATAYMDSIPLVVISGQVPTAAIGQDAFQECDTVGITRPCVKHNFLVKDVRELAETVK
KAFYIARTGRPGPVLIDIPKDISKTPCEYEPVKNVSLRSYNPVTKGHSGQIRKAVSLLLSAKRPYIYTGGGIILADASRE
LNQFADLLGYPVTNTLMGLGGYRASDRKFLGMLGMHGTYEANMAMQHCDVLIAIGARFDDRVIGDPAHFASRPRKIIHID
IDPSSISKRVKVDIPIVGDVKEVLKDLIEQLQTAEHGPDADALAQWWKDIEGWRAKDCLKYDRESEIIKPQYVVEKAWEL
TDGNAFVCSDVGQHQMWAAQFYRFNKPRRWINSGGLGTMGFGLPAAMGVKMAHPDDDVLCITGEGSIQMCIQELSTCKQY
DTPVKIISLNNRYLGMVRQWQQIEYSKRYSHSYMDALPDFVKLAQAYGHIGMRIEKSADVEPALKEALRLKDRTVFLDFQ
TDPTENVWPMVQAGKGITEMLLGSEDL

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=585, Percent_Identity=25.4700854700855, Blast_Score=171, Evalue=2e-42,
Organism=Homo sapiens, GI21361361, Length=511, Percent_Identity=27.2015655577299, Blast_Score=154, Evalue=2e-37,
Organism=Escherichia coli, GI87081685, Length=571, Percent_Identity=57.4430823117338, Blast_Score=677, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=560, Percent_Identity=40.8928571428571, Blast_Score=414, Evalue=1e-117,
Organism=Escherichia coli, GI1786717, Length=534, Percent_Identity=33.8951310861423, Blast_Score=310, Evalue=1e-85,
Organism=Escherichia coli, GI1787096, Length=565, Percent_Identity=27.0796460176991, Blast_Score=202, Evalue=4e-53,
Organism=Escherichia coli, GI1788716, Length=563, Percent_Identity=23.2682060390764, Blast_Score=140, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17531301, Length=546, Percent_Identity=26.1904761904762, Blast_Score=158, Evalue=8e-39,
Organism=Caenorhabditis elegans, GI17531299, Length=546, Percent_Identity=26.1904761904762, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17542570, Length=523, Percent_Identity=27.151051625239, Blast_Score=138, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6323755, Length=586, Percent_Identity=42.1501706484642, Blast_Score=452, Evalue=1e-128,
Organism=Saccharomyces cerevisiae, GI6320816, Length=475, Percent_Identity=24.6315789473684, Blast_Score=100, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6320123, Length=544, Percent_Identity=20.2205882352941, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6321524, Length=518, Percent_Identity=20.6563706563707, Blast_Score=77, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6323073, Length=488, Percent_Identity=21.7213114754098, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19922626, Length=567, Percent_Identity=26.1022927689594, Blast_Score=177, Evalue=2e-44,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 64964; Mature: 64964

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMPSAEFSTSESLSPQKTGSIGATVLMKALADENVEFIWGYPGGSVLYIYDELYKQDKI
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCEEEEEHHHHHHHHH
QHVLVRHEQAAVHAADAYSRSTGKVGVCLVTSGPGVTNAVTGIATAYMDSIPLVVISGQV
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCC
PTAAIGQDAFQECDTVGITRPCVKHNFLVKDVRELAETVKKAFYIARTGRPGPVLIDIPK
CCHHHCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCC
DISKTPCEYEPVKNVSLRSYNPVTKGHSGQIRKAVSLLLSAKRPYIYTGGGIILADASRE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCEEEECCHHH
LNQFADLLGYPVTNTLMGLGGYRASDRKFLGMLGMHGTYEANMAMQHCDVLIAIGARFDD
HHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHHCCCCCCHHHHHHHHCCEEEEECCCCCC
RVIGDPAHFASRPRKIIHIDIDPSSISKRVKVDIPIVGDVKEVLKDLIEQLQTAEHGPDA
CCCCCCHHHHCCCCEEEEEEECHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCH
DALAQWWKDIEGWRAKDCLKYDRESEIIKPQYVVEKAWELTDGNAFVCSDVGQHQMWAAQ
HHHHHHHHHHCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCHHHHHHH
FYRFNKPRRWINSGGLGTMGFGLPAAMGVKMAHPDDDVLCITGEGSIQMCIQELSTCKQY
HHHCCCCHHHHCCCCCCCCCCCCHHHHCEEEECCCCCEEEEECCCHHHHHHHHHHHHHHC
DTPVKIISLNNRYLGMVRQWQQIEYSKRYSHSYMDALPDFVKLAQAYGHIGMRIEKSADV
CCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
EPALKEALRLKDRTVFLDFQTDPTENVWPMVQAGKGITEMLLGSEDL
CHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHCCCCHHHHHCCCCCC
>Mature Secondary Structure
MNMPSAEFSTSESLSPQKTGSIGATVLMKALADENVEFIWGYPGGSVLYIYDELYKQDKI
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCEEEEEHHHHHHHHH
QHVLVRHEQAAVHAADAYSRSTGKVGVCLVTSGPGVTNAVTGIATAYMDSIPLVVISGQV
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCC
PTAAIGQDAFQECDTVGITRPCVKHNFLVKDVRELAETVKKAFYIARTGRPGPVLIDIPK
CCHHHCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCC
DISKTPCEYEPVKNVSLRSYNPVTKGHSGQIRKAVSLLLSAKRPYIYTGGGIILADASRE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCEEEECCHHH
LNQFADLLGYPVTNTLMGLGGYRASDRKFLGMLGMHGTYEANMAMQHCDVLIAIGARFDD
HHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHHCCCCCCHHHHHHHHCCEEEEECCCCCC
RVIGDPAHFASRPRKIIHIDIDPSSISKRVKVDIPIVGDVKEVLKDLIEQLQTAEHGPDA
CCCCCCHHHHCCCCEEEEEEECHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCH
DALAQWWKDIEGWRAKDCLKYDRESEIIKPQYVVEKAWELTDGNAFVCSDVGQHQMWAAQ
HHHHHHHHHHCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCHHHHHHH
FYRFNKPRRWINSGGLGTMGFGLPAAMGVKMAHPDDDVLCITGEGSIQMCIQELSTCKQY
HHHCCCCHHHHCCCCCCCCCCCCHHHHCEEEECCCCCEEEEECCCHHHHHHHHHHHHHHC
DTPVKIISLNNRYLGMVRQWQQIEYSKRYSHSYMDALPDFVKLAQAYGHIGMRIEKSADV
CCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
EPALKEALRLKDRTVFLDFQTDPTENVWPMVQAGKGITEMLLGSEDL
CHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHCCCCHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308579; 1630901; 9278503; 3891724; 9298646 [H]