Click here to switch to the map view.

The map label for this gene is tgt [H]

Identifier: 83721101

GI number: 83721101

Start: 1427254

End: 1428438

Strand: Reverse

Name: tgt [H]

Synonym: BTH_I1278

Alternate gene names: 83721101

Gene position: 1428438-1427254 (Counterclockwise)

Preceding gene: 83720404

Following gene: 83719987

Centisome position: 37.5

GC content: 66.41

Gene sequence:

>1185_bases
ATGACGACCGAAGGTCCATCGCACGATACGCCCACGGGCATTCGCCCGCATAACGGCCTGAAATTCGAACTGCTGACGAC
CGACGGCCGCGCGCGCCGCGGCCGCGTGACGCTCAATCACGGCGTCGTCGAGACGCCGATCTTCATGCCGGTCGGCACGT
ACGGCACCGTGAAGGCCGTGCAGCCGCGCGAGCTCGACGAGATGCGCGCGCAGATCATCCTCGGCAACACGTTCCACCTG
TGGCTGCGCCCCGGCCTCGAGACGATCGACGCGCACGGCGGGCTGCACCGCTTCATGGGCTGGAACAAGCCGATCCTCAC
CGATTCGGGCGGCTTCCAGGTGTTCTCGCTCGGCGATCTGCGCAAGATCACCGAGGAAGGCGTCACGTTCGCGTCGCCGA
TCAACGGCGACCGGCTGTTCCTGTCGCCGGAAGTGTCGATGCAGATCCAGAAGACGCTGAATTCCGACATCGTGATGCAG
TTCGACGAATGCACGCCGTACGCGACGAACGGCGTGCCGACGACGCACCGGGAAGCGGCCGAATCGATGCGGATGTCGCT
GCGCTGGGCGAAGCGCTCGCTCGCCGAGTTCGAGCGCCTCGGCAATCCGAACGCCCTGTTCGGCATCGTGCAGGGCGGCA
TGTACGAGGACCTGCGCGACGAATCGCTCGCCGGGCTCTCGGAACTCGGCTTCCACGGCCTCGCGATCGGCGGGCTGTCG
GTCGGCGAGCCGAAGGAGGACATGATGCGCGTGCTCGAGCACGTCGCGCCGCGCCTGCCCGCCGACAAGCCGCACTACCT
GATGGGCGTGGGCACGCCGGAGGATCTCGTCGCAGGCGTCGCGGCGGGCGTCGACATGTTCGACTGCGTGATGCCGACCC
GCAACGCGCGCAACGGCTGGCTGTTCACGCGCTTCGGCGACGTGAAGATCCGCAACGCGACGCACAAGAATTCGCTCAAG
CCGCTTGACGAGACCTGCGGCTGCTACACGTGCCGACATTTCTCTCGCGGCTACCTGCACCATCTGCATCGCGTCGGCGA
GATTCTCGGCGCGCAGTTGAACACGATCCACAACCTGCACTACTACCTCGAGCTGATGCGCGAGATCCGCGAGGCGATCG
AGACGCACACGTTCGAGGCGTTCCAAAAGCGCTTCGCGCAGGACCGCGCGCGCGGCGTCGACTGA

Upstream 100 bases:

>100_bases
GCGCCTGCAACATGCGCCGGAGTTGTCGATCCGGAGTCAGCGCTCGAGCGCATACTCCGGTCCCGTGCAAAGCGCTTCCG
GTGGCAAGGAGTCCAACCAG

Downstream 100 bases:

>100_bases
CGGGGCGCGACGGCGCATCGCGCGGTCGCGCCGCGCATGCCGGCCGCCGCGCCTGCCCCGCAAAACCTTACAATCATCTT
TCGCAACACGCGCGAACGCT

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MTTEGPSHDTPTGIRPHNGLKFELLTTDGRARRGRVTLNHGVVETPIFMPVGTYGTVKAVQPRELDEMRAQIILGNTFHL
WLRPGLETIDAHGGLHRFMGWNKPILTDSGGFQVFSLGDLRKITEEGVTFASPINGDRLFLSPEVSMQIQKTLNSDIVMQ
FDECTPYATNGVPTTHREAAESMRMSLRWAKRSLAEFERLGNPNALFGIVQGGMYEDLRDESLAGLSELGFHGLAIGGLS
VGEPKEDMMRVLEHVAPRLPADKPHYLMGVGTPEDLVAGVAAGVDMFDCVMPTRNARNGWLFTRFGDVKIRNATHKNSLK
PLDETCGCYTCRHFSRGYLHHLHRVGEILGAQLNTIHNLHYYLELMREIREAIETHTFEAFQKRFAQDRARGVD

Sequences:

>Translated_394_residues
MTTEGPSHDTPTGIRPHNGLKFELLTTDGRARRGRVTLNHGVVETPIFMPVGTYGTVKAVQPRELDEMRAQIILGNTFHL
WLRPGLETIDAHGGLHRFMGWNKPILTDSGGFQVFSLGDLRKITEEGVTFASPINGDRLFLSPEVSMQIQKTLNSDIVMQ
FDECTPYATNGVPTTHREAAESMRMSLRWAKRSLAEFERLGNPNALFGIVQGGMYEDLRDESLAGLSELGFHGLAIGGLS
VGEPKEDMMRVLEHVAPRLPADKPHYLMGVGTPEDLVAGVAAGVDMFDCVMPTRNARNGWLFTRFGDVKIRNATHKNSLK
PLDETCGCYTCRHFSRGYLHHLHRVGEILGAQLNTIHNLHYYLELMREIREAIETHTFEAFQKRFAQDRARGVD
>Mature_393_residues
TTEGPSHDTPTGIRPHNGLKFELLTTDGRARRGRVTLNHGVVETPIFMPVGTYGTVKAVQPRELDEMRAQIILGNTFHLW
LRPGLETIDAHGGLHRFMGWNKPILTDSGGFQVFSLGDLRKITEEGVTFASPINGDRLFLSPEVSMQIQKTLNSDIVMQF
DECTPYATNGVPTTHREAAESMRMSLRWAKRSLAEFERLGNPNALFGIVQGGMYEDLRDESLAGLSELGFHGLAIGGLSV
GEPKEDMMRVLEHVAPRLPADKPHYLMGVGTPEDLVAGVAAGVDMFDCVMPTRNARNGWLFTRFGDVKIRNATHKNSLKP
LDETCGCYTCRHFSRGYLHHLHRVGEILGAQLNTIHNLHYYLELMREIREAIETHTFEAFQKRFAQDRARGVD

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=356, Percent_Identity=44.9438202247191, Blast_Score=301, Evalue=6e-82,
Organism=Escherichia coli, GI1786607, Length=374, Percent_Identity=66.3101604278075, Blast_Score=504, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI17544680, Length=371, Percent_Identity=43.1266846361186, Blast_Score=303, Evalue=2e-82,
Organism=Caenorhabditis elegans, GI25146502, Length=138, Percent_Identity=28.9855072463768, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI19920510, Length=383, Percent_Identity=44.1253263707572, Blast_Score=323, Evalue=1e-88,
Organism=Drosophila melanogaster, GI21355311, Length=158, Percent_Identity=29.1139240506329, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 44077; Mature: 43946

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTEGPSHDTPTGIRPHNGLKFELLTTDGRARRGRVTLNHGVVETPIFMPVGTYGTVKAV
CCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEECCCEEECCEEEECCCCCCEECC
QPRELDEMRAQIILGNTFHLWLRPGLETIDAHGGLHRFMGWNKPILTDSGGFQVFSLGDL
CCCCHHHHHHEEEECCEEEEEECCCCHHHHCCCCHHHHCCCCCCEEECCCCEEEEECCHH
RKITEEGVTFASPINGDRLFLSPEVSMQIQKTLNSDIVMQFDECTPYATNGVPTTHREAA
HHHHHCCCEEECCCCCCEEEECCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHH
ESMRMSLRWAKRSLAEFERLGNPNALFGIVQGGMYEDLRDESLAGLSELGFHGLAIGGLS
HHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEECCCC
VGEPKEDMMRVLEHVAPRLPADKPHYLMGVGTPEDLVAGVAAGVDMFDCVMPTRNARNGW
CCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHCCHHHHHHCCCCCCCCCE
LFTRFGDVKIRNATHKNSLKPLDETCGCYTCRHFSRGYLHHLHRVGEILGAQLNTIHNLH
EEEEECCEEEECCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YYLELMREIREAIETHTFEAFQKRFAQDRARGVD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TTEGPSHDTPTGIRPHNGLKFELLTTDGRARRGRVTLNHGVVETPIFMPVGTYGTVKAV
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEECCCEEECCEEEECCCCCCEECC
QPRELDEMRAQIILGNTFHLWLRPGLETIDAHGGLHRFMGWNKPILTDSGGFQVFSLGDL
CCCCHHHHHHEEEECCEEEEEECCCCHHHHCCCCHHHHCCCCCCEEECCCCEEEEECCHH
RKITEEGVTFASPINGDRLFLSPEVSMQIQKTLNSDIVMQFDECTPYATNGVPTTHREAA
HHHHHCCCEEECCCCCCEEEECCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHH
ESMRMSLRWAKRSLAEFERLGNPNALFGIVQGGMYEDLRDESLAGLSELGFHGLAIGGLS
HHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEECCCC
VGEPKEDMMRVLEHVAPRLPADKPHYLMGVGTPEDLVAGVAAGVDMFDCVMPTRNARNGW
CCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHCCHHHHHHCCCCCCCCCE
LFTRFGDVKIRNATHKNSLKPLDETCGCYTCRHFSRGYLHHLHRVGEILGAQLNTIHNLH
EEEEECCEEEECCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YYLELMREIREAIETHTFEAFQKRFAQDRARGVD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA