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The map label for this gene is queA [H]

Identifier: 83720404

GI number: 83720404

Start: 1428710

End: 1429834

Strand: Reverse

Name: queA [H]

Synonym: BTH_I1279

Alternate gene names: 83720404

Gene position: 1429834-1428710 (Counterclockwise)

Preceding gene: 83719785

Following gene: 83721101

Centisome position: 37.54

GC content: 71.56

Gene sequence:

>1125_bases
GTGCCGCGACGCGCTTTGTCCGTCAGGCCGGCCGCCCCGGCCCCGTTTCGCATGCTCACGCTTTCCGATTTCGATTTCGA
TCTGCCGCCCGAGTTGATCGCGCAAACCGCGCTGCCCGAACGCAGCGCGAGCCGCCTGCTCGAGGTGGACAACGCGAGCG
CGCCGCCGCGCCTCGTCGACCGGCGCTTCGCCGAATTGCCCGCGTGCGTCGCGCCGGGCGACCTGCTCGTCTTCAACGAC
ACGAAGGTTCTGAAGGCGCGCTTCTTCGGCCGCAAGGCGAGCGGCGGCAAGATCGAGGTGCTGATCGAGCGCGTCACCGG
CCAGCGCACCGCGCTCGCGCAGATCCGCGCGAGCAAGAGCCCCGCGCCCGGCACGACGCTCACGCTCGCCGACGCGTTCG
ACGTGACGGTCGGCGAGCGCGTCGAGCCGTTCTTCACGCTGCACTTCCCCGACGATTGCCTCGTGCTGATCGAACGCCAC
GGCCGGCTGCCGCTGCCGCCGTACATCGAGCACACGCCCGACGCGACCGACGAGGCCCGCTATCAGACCGTGTTCGCCGC
GAATCCGGGCGCCGTCGCCGCGCCGACGGCCGGCCTGCACTTCGACGACGCGGTGCTCGCCGCGCTCGATGCGCGCGGCG
TCGAGCGCGCGACGCTCACGCTGCACGTCGGCGCGGGCACGTTCCAGCCGGTGCGCGTCGAGAACATCGCCGAGCACAGG
ATGCATCGCGAGTCGTACGAGCTGACCGACGCGCTCGTCGAGAAGATCGCCGCCACCCGCGCGCGCGGCGGCCGCGTGAT
CGCGGTCGGCACGACGTCGATGCGCGCGCTGGAGGCGGCCGCGCGCGACGCGGACGCCGCCGGCCGGCCGCTCGCCGCGA
CGCGGGCGGAAACCGACATCTTCATCACGCCGGGCTACCGCTTTCGTGTCGTCGACCGGCTCGTGACGAATTTCCACTTG
CCGAAATCGACGCTGCTGATGCTCGTGTCCGCATTTGCCGGCGTCGACACGATCCGCGCCGCGTACCGGCACGCGATCGA
TGCGCGCTACCGCTTCTTCAGCTACGGCGACGCGATGCTGCTCACGCGGCGCGACGCGGCCGAGGAAACGCACGGCGGCG
GGTAA

Upstream 100 bases:

>100_bases
CATCGGTCGGAAGGCTAGCAAGTACAATAGCGGCTTTCACGTCGCCCCAACGAGCGACGCCGGCCAAGCCGCGCAACCCC
GCCATCATAGCGGCTCGCGC

Downstream 100 bases:

>100_bases
GCGCGGCGGATTCGGAGGGTCGGAGATTTGTCGGCTTGGCAGTTCGGCGGCTCAGCGGCTCAGCGGCTCAGCGGCTCAGC
GGCTCAGCGGCTCAGCGGCA

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 374; Mature: 373

Protein sequence:

>374_residues
MPRRALSVRPAAPAPFRMLTLSDFDFDLPPELIAQTALPERSASRLLEVDNASAPPRLVDRRFAELPACVAPGDLLVFND
TKVLKARFFGRKASGGKIEVLIERVTGQRTALAQIRASKSPAPGTTLTLADAFDVTVGERVEPFFTLHFPDDCLVLIERH
GRLPLPPYIEHTPDATDEARYQTVFAANPGAVAAPTAGLHFDDAVLAALDARGVERATLTLHVGAGTFQPVRVENIAEHR
MHRESYELTDALVEKIAATRARGGRVIAVGTTSMRALEAAARDADAAGRPLAATRAETDIFITPGYRFRVVDRLVTNFHL
PKSTLLMLVSAFAGVDTIRAAYRHAIDARYRFFSYGDAMLLTRRDAAEETHGGG

Sequences:

>Translated_374_residues
MPRRALSVRPAAPAPFRMLTLSDFDFDLPPELIAQTALPERSASRLLEVDNASAPPRLVDRRFAELPACVAPGDLLVFND
TKVLKARFFGRKASGGKIEVLIERVTGQRTALAQIRASKSPAPGTTLTLADAFDVTVGERVEPFFTLHFPDDCLVLIERH
GRLPLPPYIEHTPDATDEARYQTVFAANPGAVAAPTAGLHFDDAVLAALDARGVERATLTLHVGAGTFQPVRVENIAEHR
MHRESYELTDALVEKIAATRARGGRVIAVGTTSMRALEAAARDADAAGRPLAATRAETDIFITPGYRFRVVDRLVTNFHL
PKSTLLMLVSAFAGVDTIRAAYRHAIDARYRFFSYGDAMLLTRRDAAEETHGGG
>Mature_373_residues
PRRALSVRPAAPAPFRMLTLSDFDFDLPPELIAQTALPERSASRLLEVDNASAPPRLVDRRFAELPACVAPGDLLVFNDT
KVLKARFFGRKASGGKIEVLIERVTGQRTALAQIRASKSPAPGTTLTLADAFDVTVGERVEPFFTLHFPDDCLVLIERHG
RLPLPPYIEHTPDATDEARYQTVFAANPGAVAAPTAGLHFDDAVLAALDARGVERATLTLHVGAGTFQPVRVENIAEHRM
HRESYELTDALVEKIAATRARGGRVIAVGTTSMRALEAAARDADAAGRPLAATRAETDIFITPGYRFRVVDRLVTNFHLP
KSTLLMLVSAFAGVDTIRAAYRHAIDARYRFFSYGDAMLLTRRDAAEETHGGG

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=348, Percent_Identity=51.7241379310345, Blast_Score=337, Evalue=5e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 40703; Mature: 40572

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRRALSVRPAAPAPFRMLTLSDFDFDLPPELIAQTALPERSASRLLEVDNASAPPRLVD
CCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHCCCHHHHHHHEECCCCCCCHHHHH
RRFAELPACVAPGDLLVFNDTKVLKARFFGRKASGGKIEVLIERVTGQRTALAQIRASKS
HHHHHCCCEECCCCEEEECCCHHHHHHHCCCCCCCCEEEEEEEECCCCHHHHHHHHCCCC
PAPGTTLTLADAFDVTVGERVEPFFTLHFPDDCLVLIERHGRLPLPPYIEHTPDATDEAR
CCCCCEEEEECCCCCCCCCCCCCEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCCC
YQTVFAANPGAVAAPTAGLHFDDAVLAALDARGVERATLTLHVGAGTFQPVRVENIAEHR
EEEEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEEEECCCCCCCEEHHHHHHHH
MHRESYELTDALVEKIAATRARGGRVIAVGTTSMRALEAAARDADAAGRPLAATRAETDI
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHCCCCCCCCCCEECCCCCEE
FITPGYRFRVVDRLVTNFHLPKSTLLMLVSAFAGVDTIRAAYRHAIDARYRFFSYGDAML
EECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHEEEEECCCEEE
LTRRDAAEETHGGG
EEECCHHCCCCCCC
>Mature Secondary Structure 
PRRALSVRPAAPAPFRMLTLSDFDFDLPPELIAQTALPERSASRLLEVDNASAPPRLVD
CCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHCCCHHHHHHHEECCCCCCCHHHHH
RRFAELPACVAPGDLLVFNDTKVLKARFFGRKASGGKIEVLIERVTGQRTALAQIRASKS
HHHHHCCCEECCCCEEEECCCHHHHHHHCCCCCCCCEEEEEEEECCCCHHHHHHHHCCCC
PAPGTTLTLADAFDVTVGERVEPFFTLHFPDDCLVLIERHGRLPLPPYIEHTPDATDEAR
CCCCCEEEEECCCCCCCCCCCCCEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCCC
YQTVFAANPGAVAAPTAGLHFDDAVLAALDARGVERATLTLHVGAGTFQPVRVENIAEHR
EEEEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEEEECCCCCCCEEHHHHHHHH
MHRESYELTDALVEKIAATRARGGRVIAVGTTSMRALEAAARDADAAGRPLAATRAETDI
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHCCCCCCCCCCEECCCCCEE
FITPGYRFRVVDRLVTNFHLPKSTLLMLVSAFAGVDTIRAAYRHAIDARYRFFSYGDAML
EECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHEEEEECCCEEE
LTRRDAAEETHGGG
EEECCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA