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The map label for this gene is thyA [H]

Identifier: 83720929

GI number: 83720929

Start: 1889293

End: 1890264

Strand: Direct

Name: thyA [H]

Synonym: BTH_I1680

Alternate gene names: 83720929

Gene position: 1889293-1890264 (Clockwise)

Preceding gene: 83719176

Following gene: 83721651

Centisome position: 49.6

GC content: 65.64

Gene sequence:

>972_bases
ATGAAACAGTATCTCGATCTCGTTCGCACCATTCTCGACACCGGCACCTGGCAGAGCAACCGCACGGGCATCCGCACGAT
CGGCATCCCGGGCGCGATGCTGCGCTTCGATCTGCAACAGGGCTTTCCCGCCGTGACGACGAAGAAGCTCGCGTTCAAGT
CGGCGATCGGCGAGCTCGTCGGCTTCCTGCGCGCGACGCGCAGCGCGGCGGAGTTTCGCGCGCTCGGCTGCAAGGTGTGG
GACGCGAATGCGAATGAGAACGCGCAGTGGCTCGCGAATCCGTATCGCCGCGGCGCCGACGATCTCGGCGACGTGTACGG
CGTCCAATGGCGGCGCTGGCCGGGCTACAAGGTGCTCGACGCGCACGCCGACGCGCAGATCGCCGATGCGACTTCGCGCG
GCTTTCGCATCGTCGCGCGCTTCGAGGAGGGCGGCGCGGACAAGGTGCTGCTCCACAAGGCGATCGATCAACTGCGCGAT
TGCCTCGACACGATCGTGCGCGATCCGTCGAGCCGGCGCATCCTGTTCCACGGCTGGAATCCCGCGGTGCTCGACGAGAT
CGCGCTGCCCGCATGCCATCTGCTGTATCAGTTCCTGCCGAACGTCGAGCGGCGCGAAATCTCGCTGTGCCTGTACATCC
GCAGCAACGACGTCGGACTCGGCACGCCGTTCAATCTCGCCGAAGGCGCGGCGCTCCTCACGCTCGTCGGCCGGCTGACG
GGCTACTCGCCGCGCTGGTTCACGTACTTTATCGGCGATGCGCACATCTACGAGAATCAGCTCGACATGCTCAAGCAGCA
ACTCGAGCGCGAGCCGTTCGAGAGTCCGCGGCTCGAGCTTGCCGAGCGCGTGCCCGATTACGCGAAGACGGGCAAGTACG
AGCCGCAATGGCTCGAACGCGTCGAGCCGTCGGATTTCACGCTCGTCGGCTATCGCCATCATGAACCGCTGAGCGCGCCG
ATGGCCGTCTGA

Upstream 100 bases:

>100_bases
GCACGCGCGCGAAGCGCGACGAGGATCTGCGCCGGGCCTGCTAGCGCGGGCCTTGCTAGAATGGCGGGCACGGCCGCTTT
TCGCGGCCGGAGGAACCCGC

Downstream 100 bases:

>100_bases
ACGACGCGCGGCCCGTCTCGATCGATCCATGAAAAACCCGCGAATTCGCGGGTTTTTTCGTTGATGCGGCGAGTGCGGTG
CGCTTACGCGTGCCGGCGCT

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MKQYLDLVRTILDTGTWQSNRTGIRTIGIPGAMLRFDLQQGFPAVTTKKLAFKSAIGELVGFLRATRSAAEFRALGCKVW
DANANENAQWLANPYRRGADDLGDVYGVQWRRWPGYKVLDAHADAQIADATSRGFRIVARFEEGGADKVLLHKAIDQLRD
CLDTIVRDPSSRRILFHGWNPAVLDEIALPACHLLYQFLPNVERREISLCLYIRSNDVGLGTPFNLAEGAALLTLVGRLT
GYSPRWFTYFIGDAHIYENQLDMLKQQLEREPFESPRLELAERVPDYAKTGKYEPQWLERVEPSDFTLVGYRHHEPLSAP
MAV

Sequences:

>Translated_323_residues
MKQYLDLVRTILDTGTWQSNRTGIRTIGIPGAMLRFDLQQGFPAVTTKKLAFKSAIGELVGFLRATRSAAEFRALGCKVW
DANANENAQWLANPYRRGADDLGDVYGVQWRRWPGYKVLDAHADAQIADATSRGFRIVARFEEGGADKVLLHKAIDQLRD
CLDTIVRDPSSRRILFHGWNPAVLDEIALPACHLLYQFLPNVERREISLCLYIRSNDVGLGTPFNLAEGAALLTLVGRLT
GYSPRWFTYFIGDAHIYENQLDMLKQQLEREPFESPRLELAERVPDYAKTGKYEPQWLERVEPSDFTLVGYRHHEPLSAP
MAV
>Mature_323_residues
MKQYLDLVRTILDTGTWQSNRTGIRTIGIPGAMLRFDLQQGFPAVTTKKLAFKSAIGELVGFLRATRSAAEFRALGCKVW
DANANENAQWLANPYRRGADDLGDVYGVQWRRWPGYKVLDAHADAQIADATSRGFRIVARFEEGGADKVLLHKAIDQLRD
CLDTIVRDPSSRRILFHGWNPAVLDEIALPACHLLYQFLPNVERREISLCLYIRSNDVGLGTPFNLAEGAALLTLVGRLT
GYSPRWFTYFIGDAHIYENQLDMLKQQLEREPFESPRLELAERVPDYAKTGKYEPQWLERVEPSDFTLVGYRHHEPLSAP
MAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507751, Length=321, Percent_Identity=34.5794392523364, Blast_Score=184, Evalue=1e-46,
Organism=Escherichia coli, GI1789191, Length=323, Percent_Identity=34.984520123839, Blast_Score=176, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI71993377, Length=283, Percent_Identity=33.9222614840989, Blast_Score=164, Evalue=8e-41,
Organism=Saccharomyces cerevisiae, GI6324648, Length=330, Percent_Identity=27.5757575757576, Blast_Score=131, Evalue=1e-31,
Organism=Drosophila melanogaster, GI17137556, Length=321, Percent_Identity=35.5140186915888, Blast_Score=194, Evalue=7e-50,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 36623; Mature: 36623

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQYLDLVRTILDTGTWQSNRTGIRTIGIPGAMLRFDLQQGFPAVTTKKLAFKSAIGELV
CHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHEEHHCCCCCHHHHHHHHHHHHHHHH
GFLRATRSAAEFRALGCKVWDANANENAQWLANPYRRGADDLGDVYGVQWRRWPGYKVLD
HHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHCHHHCCCHHHHHHHCCCEEECCCCEEEE
AHADAQIADATSRGFRIVARFEEGGADKVLLHKAIDQLRDCLDTIVRDPSSRRILFHGWN
CCCCCHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCC
PAVLDEIALPACHLLYQFLPNVERREISLCLYIRSNDVGLGTPFNLAEGAALLTLVGRLT
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHC
GYSPRWFTYFIGDAHIYENQLDMLKQQLEREPFESPRLELAERVPDYAKTGKYEPQWLER
CCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHCCCCCHHHHHH
VEPSDFTLVGYRHHEPLSAPMAV
CCCCCEEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MKQYLDLVRTILDTGTWQSNRTGIRTIGIPGAMLRFDLQQGFPAVTTKKLAFKSAIGELV
CHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHEEHHCCCCCHHHHHHHHHHHHHHHH
GFLRATRSAAEFRALGCKVWDANANENAQWLANPYRRGADDLGDVYGVQWRRWPGYKVLD
HHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHCHHHCCCHHHHHHHCCCEEECCCCEEEE
AHADAQIADATSRGFRIVARFEEGGADKVLLHKAIDQLRDCLDTIVRDPSSRRILFHGWN
CCCCCHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCC
PAVLDEIALPACHLLYQFLPNVERREISLCLYIRSNDVGLGTPFNLAEGAALLTLVGRLT
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHC
GYSPRWFTYFIGDAHIYENQLDMLKQQLEREPFESPRLELAERVPDYAKTGKYEPQWLER
CCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHCCCCCHHHHHH
VEPSDFTLVGYRHHEPLSAPMAV
CCCCCEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12910271 [H]