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The map label for this gene is mhpA [H]

Identifier: 83720631

GI number: 83720631

Start: 1567921

End: 1569582

Strand: Direct

Name: mhpA [H]

Synonym: BTH_I1395

Alternate gene names: 83720631

Gene position: 1567921-1569582 (Clockwise)

Preceding gene: 83719630

Following gene: 83719999

Centisome position: 41.16

GC content: 70.76

Gene sequence:

>1662_bases
ATGACCATCGATTATCAGACGCTGAAGTTCGACTATCGTCCGCGCGCGGCGCTTGCCGATGCGGGCGAGCCGCATCCGGC
CGTCGTCGTCGGCGCGGGGCCCGTCGGGCTCGCGGCGGCGATCGATCTCGCGCAGCACGGCGTGCCCGTCGTGCTGCTCG
ACGACGACGACACGCTGTCGAGCGGCTCGCGCGCGATCTGCTTCGCGAAGCGCACGCTCGAGATCTTCGATCGCCTCGGC
TGCGGCGAGCGGGTCGCGCAAAAGGGCGTGAGCTGGCATGTCGGCAAGGTGTTCCTGCAGGACGAGCTGATCTACGCGTT
CGACCTGCTGCCGGAAGCCGGGCACGCGCGGCCCGCGTTCGTCAATCTGCAGCAGTATTACGTCGAAGGCTATCTGGCCG
AGCGTGCGCTCGAGCTGCCGAACCTCGATCTGCGCTGGAAGAGCCGCGTGACGGGCATTCGGCAGACGCCCGAGCACGCG
GAGCTCGACGTCGACACGCCGGAGGGCCCGTACACGCTGCGCGCGCGCTACGTGATCGCGGCGGACGGCTCGAAGAGCCC
GCTGCGCGCGATGATGGGGCTCGACAGCCACGGCCGCACGTTCAAGGACCGCTTCCTGATCGCCGACGTGAAGATGAAGG
CGCCGTTCCCGGCCGAGCGCTGGTTCTGGTTCGATCCGCCGTTCCATCGCAATCAATCGGTGCTGCTGCACCGGCAGCCG
GACGACATGTGGCGCATCGATTTCCAGCTCGGCTGGGACGCCGATCCCGTCGCGGAGAAGGCGCCGGAGCGCGTGATTCC
GCGCGTGCGCGCGCTGCTCGGGCCGAACGTCGAGTTCGAGCTCGAATGGGTGAGCGTCTATACGTTCCGCTGCCAGCGGA
TGGACAGCTTCCGCCACGGCCGCGTGCTGTTCGCGGGCGATTCCGCGCATGGCGTGTCGCCGTTCGGCGCGCGCGGCGCG
AACAGCGGCGTGCAGGATGCGGAGAACCTCGCGTGGAAGCTCGCGCTCGTGCTCGACGGCCGCGCGCCCGACCGTCTGCT
CGACACGTATGCGCGCGAGCGCGAATTCGCCGCCGACGAGAACATCCGCAATTCGACCCGCTCGACCGACTTCATCACGC
CGAAGAGCCCGATCTCGCGCGTGTTCCGCGACGCGACGCTCAAGCTCGCGCGCGACTGCGAGTTCGCGCGCAGGCTCGTC
AACAGCGGCCGGCTATCGGTGCCGGCCGTGCTCGCCGATTCGCCGCTGAACACGCCCGATCGCGCGGGCGAGACGTTCGA
GCGCGCGCCGGCGCCGGGCGCGGTCGCGCCCGATGCACCCGTGCTCGCGAACGGCGAGCCCGGCTGGCTGCTGCAGCATC
TGAAGGGCGGCTTCGTCGGCATCCTGTTCGGCTCGATCGGCGACGCGGGCGAGCTCGTCGCCGCGACGCGCGGCCTCGCG
CCGCCGGTCGCGCCCGTGCTCGTCGTGCCGAAGGGCGAGGCGCGCGCGGTCGAGGGCGTCGACGTGATCGAGGACAGCGA
AGGGCTCGCCGCGCAGCGCTATGACGCGCGCCCCGGCACGTTCTATCTGCTGCGTCCGGACCAGCATGTGTGCGCGCGCT
TTCGCACGGCGCAAGCGAGCGCGGTGCGCGCGGCGCTCGCCCGCGCGATCTGCCGCGCGTGA

Upstream 100 bases:

>100_bases
GACGAGGCCACGCAATATCCGGATCCGCGGATCTGGACGGCCGAGCGCGACAAGGAAATGTGGCGGACCCTCGAAGGCTG
AAGCACGGCGGGCGGCGATC

Downstream 100 bases:

>100_bases
GCGGAAAACCAACGAACGAGACCAGGAGACACCCGATGCACCTCGACACCCGCTTGCGCCTGCCCGATCCGGACGCGTTC
TACGAAGCGCTGATCGACAT

Product: FAD-dependent oxidoreductase

Products: H2O; 3-(2,3-dihydroxyphenyl)propionate; NAD [C]

Alternate protein names: 3-HCI hydroxylase; 3-HPP hydroxylase [H]

Number of amino acids: Translated: 553; Mature: 552

Protein sequence:

>553_residues
MTIDYQTLKFDYRPRAALADAGEPHPAVVVGAGPVGLAAAIDLAQHGVPVVLLDDDDTLSSGSRAICFAKRTLEIFDRLG
CGERVAQKGVSWHVGKVFLQDELIYAFDLLPEAGHARPAFVNLQQYYVEGYLAERALELPNLDLRWKSRVTGIRQTPEHA
ELDVDTPEGPYTLRARYVIAADGSKSPLRAMMGLDSHGRTFKDRFLIADVKMKAPFPAERWFWFDPPFHRNQSVLLHRQP
DDMWRIDFQLGWDADPVAEKAPERVIPRVRALLGPNVEFELEWVSVYTFRCQRMDSFRHGRVLFAGDSAHGVSPFGARGA
NSGVQDAENLAWKLALVLDGRAPDRLLDTYAREREFAADENIRNSTRSTDFITPKSPISRVFRDATLKLARDCEFARRLV
NSGRLSVPAVLADSPLNTPDRAGETFERAPAPGAVAPDAPVLANGEPGWLLQHLKGGFVGILFGSIGDAGELVAATRGLA
PPVAPVLVVPKGEARAVEGVDVIEDSEGLAAQRYDARPGTFYLLRPDQHVCARFRTAQASAVRAALARAICRA

Sequences:

>Translated_553_residues
MTIDYQTLKFDYRPRAALADAGEPHPAVVVGAGPVGLAAAIDLAQHGVPVVLLDDDDTLSSGSRAICFAKRTLEIFDRLG
CGERVAQKGVSWHVGKVFLQDELIYAFDLLPEAGHARPAFVNLQQYYVEGYLAERALELPNLDLRWKSRVTGIRQTPEHA
ELDVDTPEGPYTLRARYVIAADGSKSPLRAMMGLDSHGRTFKDRFLIADVKMKAPFPAERWFWFDPPFHRNQSVLLHRQP
DDMWRIDFQLGWDADPVAEKAPERVIPRVRALLGPNVEFELEWVSVYTFRCQRMDSFRHGRVLFAGDSAHGVSPFGARGA
NSGVQDAENLAWKLALVLDGRAPDRLLDTYAREREFAADENIRNSTRSTDFITPKSPISRVFRDATLKLARDCEFARRLV
NSGRLSVPAVLADSPLNTPDRAGETFERAPAPGAVAPDAPVLANGEPGWLLQHLKGGFVGILFGSIGDAGELVAATRGLA
PPVAPVLVVPKGEARAVEGVDVIEDSEGLAAQRYDARPGTFYLLRPDQHVCARFRTAQASAVRAALARAICRA
>Mature_552_residues
TIDYQTLKFDYRPRAALADAGEPHPAVVVGAGPVGLAAAIDLAQHGVPVVLLDDDDTLSSGSRAICFAKRTLEIFDRLGC
GERVAQKGVSWHVGKVFLQDELIYAFDLLPEAGHARPAFVNLQQYYVEGYLAERALELPNLDLRWKSRVTGIRQTPEHAE
LDVDTPEGPYTLRARYVIAADGSKSPLRAMMGLDSHGRTFKDRFLIADVKMKAPFPAERWFWFDPPFHRNQSVLLHRQPD
DMWRIDFQLGWDADPVAEKAPERVIPRVRALLGPNVEFELEWVSVYTFRCQRMDSFRHGRVLFAGDSAHGVSPFGARGAN
SGVQDAENLAWKLALVLDGRAPDRLLDTYAREREFAADENIRNSTRSTDFITPKSPISRVFRDATLKLARDCEFARRLVN
SGRLSVPAVLADSPLNTPDRAGETFERAPAPGAVAPDAPVLANGEPGWLLQHLKGGFVGILFGSIGDAGELVAATRGLAP
PVAPVLVVPKGEARAVEGVDVIEDSEGLAAQRYDARPGTFYLLRPDQHVCARFRTAQASAVRAALARAICRA

Specific function: Catalyzes the insertion of one atom of molecular oxygen into position 2 of the phenyl ring of 3-(3- hydroxyphenyl)propionate (3-HPP) and hydroxycinnamic acid (3HCI) [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=517, Percent_Identity=25.7253384912959, Blast_Score=129, Evalue=6e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 60828; Mature: 60696

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIDYQTLKFDYRPRAALADAGEPHPAVVVGAGPVGLAAAIDLAQHGVPVVLLDDDDTLS
CCCCCEEEEECCCCCHHHCCCCCCCCEEEECCCCHHHHHHHHHHHCCCEEEEECCCCCCC
SGSRAICFAKRTLEIFDRLGCGERVAQKGVSWHVGKVFLQDELIYAFDLLPEAGHARPAF
CCCEEEEEHHHHHHHHHHCCCCHHHHHCCCCEEEEEEEECCCEEEEEECCCCCCCCCCEE
VNLQQYYVEGYLAERALELPNLDLRWKSRVTGIRQTPEHAELDVDTPEGPYTLRARYVIA
EHHHHHHHHHHHHHHHHCCCCCCEEHHHHCCCCCCCCCCCEEECCCCCCCEEEEEEEEEE
ADGSKSPLRAMMGLDSHGRTFKDRFLIADVKMKAPFPAERWFWFDPPFHRNQSVLLHRQP
ECCCCCHHHHHHCCCCCCCCCCCEEEEEEEEECCCCCCCCEEEECCCCCCCCEEEEEECC
DDMWRIDFQLGWDADPVAEKAPERVIPRVRALLGPNVEFELEWVSVYTFRCQRMDSFRHG
CCEEEEEEEECCCCCHHHHHCHHHHHHHHHHHHCCCCEEEEEEEEEEEHHHHHHCCCCCC
RVLFAGDSAHGVSPFGARGANSGVQDAENLAWKLALVLDGRAPDRLLDTYAREREFAADE
EEEEECCCCCCCCCCCCCCCCCCCCHHHHHEEEEEEEECCCCCHHHHHHHHHHHHHHCCC
NIRNSTRSTDFITPKSPISRVFRDATLKLARDCEFARRLVNSGRLSVPAVLADSPLNTPD
CHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCC
RAGETFERAPAPGAVAPDAPVLANGEPGWLLQHLKGGFVGILFGSIGDAGELVAATRGLA
CCCCHHHHCCCCCCCCCCCCEEECCCCCHHHHHHCCCEEEEEECCCCCCHHHHHHHCCCC
PPVAPVLVVPKGEARAVEGVDVIEDSEGLAAQRYDARPGTFYLLRPDQHVCARFRTAQAS
CCCCCEEEEECCCCCEECCCEEEECCCCCEEEECCCCCCEEEEECCCHHHHHHHHHHHHH
AVRAALARAICRA
HHHHHHHHHHHCC
>Mature Secondary Structure 
TIDYQTLKFDYRPRAALADAGEPHPAVVVGAGPVGLAAAIDLAQHGVPVVLLDDDDTLS
CCCCEEEEECCCCCHHHCCCCCCCCEEEECCCCHHHHHHHHHHHCCCEEEEECCCCCCC
SGSRAICFAKRTLEIFDRLGCGERVAQKGVSWHVGKVFLQDELIYAFDLLPEAGHARPAF
CCCEEEEEHHHHHHHHHHCCCCHHHHHCCCCEEEEEEEECCCEEEEEECCCCCCCCCCEE
VNLQQYYVEGYLAERALELPNLDLRWKSRVTGIRQTPEHAELDVDTPEGPYTLRARYVIA
EHHHHHHHHHHHHHHHHCCCCCCEEHHHHCCCCCCCCCCCEEECCCCCCCEEEEEEEEEE
ADGSKSPLRAMMGLDSHGRTFKDRFLIADVKMKAPFPAERWFWFDPPFHRNQSVLLHRQP
ECCCCCHHHHHHCCCCCCCCCCCEEEEEEEEECCCCCCCCEEEECCCCCCCCEEEEEECC
DDMWRIDFQLGWDADPVAEKAPERVIPRVRALLGPNVEFELEWVSVYTFRCQRMDSFRHG
CCEEEEEEEECCCCCHHHHHCHHHHHHHHHHHHCCCCEEEEEEEEEEEHHHHHHCCCCCC
RVLFAGDSAHGVSPFGARGANSGVQDAENLAWKLALVLDGRAPDRLLDTYAREREFAADE
EEEEECCCCCCCCCCCCCCCCCCCCHHHHHEEEEEEEECCCCCHHHHHHHHHHHHHHCCC
NIRNSTRSTDFITPKSPISRVFRDATLKLARDCEFARRLVNSGRLSVPAVLADSPLNTPD
CHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCC
RAGETFERAPAPGAVAPDAPVLANGEPGWLLQHLKGGFVGILFGSIGDAGELVAATRGLA
CCCCHHHHCCCCCCCCCCCCEEECCCCCHHHHHHCCCEEEEEECCCCCCHHHHHHHCCCC
PPVAPVLVVPKGEARAVEGVDVIEDSEGLAAQRYDARPGTFYLLRPDQHVCARFRTAQAS
CCCCCEEEEECCCCCEECCCEEEECCCCCEEEECCCCCCEEEEECCCHHHHHHHHHHHHH
AVRAALARAICRA
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-(3-hydroxyphenyl)propionate; NADH; O2 [C]

Specific reaction: 3-(3-hydroxyphenyl)propionate + NADH + O2 = H2O + 3-(2,3-dihydroxyphenyl)propionate + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA