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The map label for this gene is trmD [H]

Identifier: 83720405

GI number: 83720405

Start: 1872512

End: 1873306

Strand: Direct

Name: trmD [H]

Synonym: BTH_I1663

Alternate gene names: 83720405

Gene position: 1872512-1873306 (Clockwise)

Preceding gene: 83719306

Following gene: 83719331

Centisome position: 49.16

GC content: 67.8

Gene sequence:

>795_bases
ATGGACGAAGCCACGCAGCGCGCGATCCAGTTCGACGTCGTCACACTCTTTCCGGAGATGTTTCGTGCGTTGACCGACTG
GGGCATCACGAGCCGCGCGGTGAAGCAGGGGCGGTTCGGCTTGCGCACGTGGAATCCGCGCGATTTCACGAGCGACAACT
ACCGCACCGTCGACGATCGTCCGTACGGCGGCGGTCCCGGCATGGTGATGCTCGCGAAGCCGCTCGAGGCGGCGATCGGT
GCGGCGAAGGCCGCACAGGCGGCGCAAGGCGTTGCGACGTCGCGCGTGGTGATGATGTCGCCGCAGGGCGCGCCGCTCAC
GCACGAGCGCGTCGCTCGGATGGCTGCGGAGCCGGGCGTCGTGTTGCTGTGCGGGCGTTACGAGGCGATCGACCAGCGCC
TGATCGACCGTTGCGTCGACGAGGAGCTGAGCCTTGGCGATTTTGTGTTGTCGGGCGGCGAGTTGCCGGCGATGGCGCTG
ATGGACGCCGTCGTGCGACTGCTGCCGGGCGTGCTGAACGATGCGCAATCGGCAGTGCAGGACAGCTTCGCCGACGGGCT
GCTCGACTGTCCGCATTACACGCGTCCCGAGGAGTACGAGGGCGTGCGCGTGCCGGACGTGCTGCTCGGCGGGCATCATG
CCGAGATCGAGCGCTGGCGCCGGCAGGAAGCGTTGAGAAACACCGTCGCGAAGCGCCCCGACCTGATTGCGCGGGCGCGT
CGCGAAAAGTTGCTGAGCCGGGCCGACGAGGCGTGGCTCGCAAGTCTCGCGAAAGAAGCGAAGCAGGCCTCCTGA

Upstream 100 bases:

>100_bases
ACGACCGGCGAGCGGCTGATTCCGTTCGTCGGCGTGTACGTGAAGGCGGTCGAGCAGGCGGCGGGACGCATCGTCGTCGA
CTGGGAAGCCGATTACTGAA

Downstream 100 bases:

>100_bases
GGCGGCGGCGCTTTCTGTTTGCGAATTTGGCGGTGCCGAGCATGCGTGTGCGGCACCTGATGTGAATCCATCCTCTATCG
GGGCCGAGCCTGGAAGCAGG

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MDEATQRAIQFDVVTLFPEMFRALTDWGITSRAVKQGRFGLRTWNPRDFTSDNYRTVDDRPYGGGPGMVMLAKPLEAAIG
AAKAAQAAQGVATSRVVMMSPQGAPLTHERVARMAAEPGVVLLCGRYEAIDQRLIDRCVDEELSLGDFVLSGGELPAMAL
MDAVVRLLPGVLNDAQSAVQDSFADGLLDCPHYTRPEEYEGVRVPDVLLGGHHAEIERWRRQEALRNTVAKRPDLIARAR
REKLLSRADEAWLASLAKEAKQAS

Sequences:

>Translated_264_residues
MDEATQRAIQFDVVTLFPEMFRALTDWGITSRAVKQGRFGLRTWNPRDFTSDNYRTVDDRPYGGGPGMVMLAKPLEAAIG
AAKAAQAAQGVATSRVVMMSPQGAPLTHERVARMAAEPGVVLLCGRYEAIDQRLIDRCVDEELSLGDFVLSGGELPAMAL
MDAVVRLLPGVLNDAQSAVQDSFADGLLDCPHYTRPEEYEGVRVPDVLLGGHHAEIERWRRQEALRNTVAKRPDLIARAR
REKLLSRADEAWLASLAKEAKQAS
>Mature_264_residues
MDEATQRAIQFDVVTLFPEMFRALTDWGITSRAVKQGRFGLRTWNPRDFTSDNYRTVDDRPYGGGPGMVMLAKPLEAAIG
AAKAAQAAQGVATSRVVMMSPQGAPLTHERVARMAAEPGVVLLCGRYEAIDQRLIDRCVDEELSLGDFVLSGGELPAMAL
MDAVVRLLPGVLNDAQSAVQDSFADGLLDCPHYTRPEEYEGVRVPDVLLGGHHAEIERWRRQEALRNTVAKRPDLIARAR
REKLLSRADEAWLASLAKEAKQAS

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=224, Percent_Identity=57.1428571428571, Blast_Score=262, Evalue=1e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 29041; Mature: 29041

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDEATQRAIQFDVVTLFPEMFRALTDWGITSRAVKQGRFGLRTWNPRDFTSDNYRTVDDR
CCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCC
PYGGGPGMVMLAKPLEAAIGAAKAAQAAQGVATSRVVMMSPQGAPLTHERVARMAAEPGV
CCCCCCCEEEEHHHHHHHHHHHHHHHHHHCHHHCEEEEECCCCCCCCHHHHHHHHCCCCE
VLLCGRYEAIDQRLIDRCVDEELSLGDFVLSGGELPAMALMDAVVRLLPGVLNDAQSAVQ
EEEECCHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DSFADGLLDCPHYTRPEEYEGVRVPDVLLGGHHAEIERWRRQEALRNTVAKRPDLIARAR
HHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHH
REKLLSRADEAWLASLAKEAKQAS
HHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDEATQRAIQFDVVTLFPEMFRALTDWGITSRAVKQGRFGLRTWNPRDFTSDNYRTVDDR
CCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCC
PYGGGPGMVMLAKPLEAAIGAAKAAQAAQGVATSRVVMMSPQGAPLTHERVARMAAEPGV
CCCCCCCEEEEHHHHHHHHHHHHHHHHHHCHHHCEEEEECCCCCCCCHHHHHHHHCCCCE
VLLCGRYEAIDQRLIDRCVDEELSLGDFVLSGGELPAMALMDAVVRLLPGVLNDAQSAVQ
EEEECCHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DSFADGLLDCPHYTRPEEYEGVRVPDVLLGGHHAEIERWRRQEALRNTVAKRPDLIARAR
HHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHH
REKLLSRADEAWLASLAKEAKQAS
HHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA