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The map label for this gene is pcpB [H]

Identifier: 83720382

GI number: 83720382

Start: 1824959

End: 1826605

Strand: Direct

Name: pcpB [H]

Synonym: BTH_I1620

Alternate gene names: 83720382

Gene position: 1824959-1826605 (Clockwise)

Preceding gene: 83718592

Following gene: 83721289

Centisome position: 47.91

GC content: 70.37

Gene sequence:

>1647_bases
ATGGCAGTCGTTCCGCTCATCACGCCTCCCGTGCTGATCGTCGGCGCCGGGCCGACCGGGCTTGCCGCCGCGCTTTGCCT
CGCGCGCGCCCGCGTGCCGGTGCGCATCGTCGACAAGGCGGCACGGCCCGCGCACCATTCGCGCGCGATCGGCATCCAGG
CCAGAACGCTCGAACTCCTCGAGCAGCAGCGCGTCGTCGACCGGTTCGTCGAGCTTGGCCATCGCGCGCGGCTCGCGATC
CTGTACTCGGCCGGCCAGCGCATCGCCGAGCTCGACTTCGATCCGCTGCAAACGCGCTATCCGTACCTGCTGTTTCTCGA
CCAGACCGTCACCGAGCGTTTGCTGACCGAGCATCTCGCGACGTTCGACGTCCACGTCGAGCGCGGCGTGACGCTCACGC
ATTGCACGGAGCCGGAAGGCGCGCTCGACGTGCGGCTGCGCCACGGCGACGGTCGCGACGAGCATCTGCAGCCGTCGTAC
GTCGTCGCGGCCGACGGCGCGCACAGCACCGTCCGGCGTCTGCTCGACGTCGATTTCGTCGGGCATGCGTTCGAGCAGAC
ATTCTTGCTCGCGGATCTCGACGTCGGCGCCGACTGGCCCGACGACGAGATTCATTTGTTCACGACGGGCGACGGCATCG
CCGGATTGTTTCCGATGGGCAACGGCCGCTACCGGCTCGTCGCCGATCGGCCGCCGCGCAACGGCGCGCTCGACGACGAG
CACGGGCCGTCGCTCGAGCTGTGCCGCGAGATCGTGCGCGCGCGCGTGACGCCCAATCTGAAGATCGGCGATCTCGCATG
GTCGTCGTATTTTCATCTGCACAGCCGGATGGTCGCGAAGCTGCGCGTCGGACGCGTGTTTTTCGCGGGCGATGCCGCGC
ACGTGCACAGCCCGGCGGGCGCGCAGGGGATGAACACGGGCATTCAGGAGGCGTTCAATCTCGGCTGGAAGCTCGCGCGC
GTGCTGGCCGGCAACGCGCCCGAGCGGTTGCTCGACACCTATCACGAGGAGCGTCACCCGATCGAGCGCGACGTGCTTCG
GCAGACGAGCTTCGCGACGCAGGTCGTCGAGGCGGACCGCGGGCCGCTCAGGCTGCTGCGCAACCACGTCGTGCCGATCC
TCGCGTCGTTCGGGCCGTTGCGGGACGCGGCGCGTCGCTCGGTCAGCGAGCTCGCGATCCAGTATCGGAAGAGCCCGCTC
ACGCTCGAACGCGTGCTCGACGGCGGGCCGCGCGCGGGCGAGCGCGCACCGGACGCGCTCTTGCATGTGCTCGACGGGCC
GCTCGGCAAGGCGCCCGGCGTCGCGCGCCTCTTCGATCTGCACGATCCCGCGCATTTCACGCTGCTCGTGCTCGAACCGC
GCGAGGCGATCGACGACACGTTGCCGTCGGATCCGGGCGAGGACGGCCGCGCGCTCGCCGACGCGCTCGAGCGGATCATG
CCGGGCGCGGTGCGCTGCTGGCGCGTGACGGATGCGCAGGGCGAGGGCGCGCCGCTGTTGATCGATGCGTACGGGCGCTC
GCGGCCCGTGTTCTACCTGCTGCGCCCGGACGGTTACGTCGCCGCGCGCGGCCGCCCGGCGACGGATGCGAACGCGCTGC
TGCGTCATTGCGAGACGTGGTTCTCGGGAATGCGGCTCGACGCGTGA

Upstream 100 bases:

>100_bases
TCGAACCGCGCCGGCCGAATCGACCGAGGCGGCCCGCCCGCTATGGATGCGGCGCGGCGGCGGGCGTAGACTGCGGGTGT
TGCGTATCGACGAGGTCCTT

Downstream 100 bases:

>100_bases
ACGCGACGGCGCGCCAGACGGGCGCGGCCGGCTCGCGGATGCGGCCCGTTGCGGACCGAGCGGCGCCGCACGCGAGCGGC
GGGGGAGCGGCCGGCGTGCG

Product: pentachlorophenol 4-monooxygenase; PcpB

Products: NA

Alternate protein names: Pentachlorophenol hydroxylase [H]

Number of amino acids: Translated: 548; Mature: 547

Protein sequence:

>548_residues
MAVVPLITPPVLIVGAGPTGLAAALCLARARVPVRIVDKAARPAHHSRAIGIQARTLELLEQQRVVDRFVELGHRARLAI
LYSAGQRIAELDFDPLQTRYPYLLFLDQTVTERLLTEHLATFDVHVERGVTLTHCTEPEGALDVRLRHGDGRDEHLQPSY
VVAADGAHSTVRRLLDVDFVGHAFEQTFLLADLDVGADWPDDEIHLFTTGDGIAGLFPMGNGRYRLVADRPPRNGALDDE
HGPSLELCREIVRARVTPNLKIGDLAWSSYFHLHSRMVAKLRVGRVFFAGDAAHVHSPAGAQGMNTGIQEAFNLGWKLAR
VLAGNAPERLLDTYHEERHPIERDVLRQTSFATQVVEADRGPLRLLRNHVVPILASFGPLRDAARRSVSELAIQYRKSPL
TLERVLDGGPRAGERAPDALLHVLDGPLGKAPGVARLFDLHDPAHFTLLVLEPREAIDDTLPSDPGEDGRALADALERIM
PGAVRCWRVTDAQGEGAPLLIDAYGRSRPVFYLLRPDGYVAARGRPATDANALLRHCETWFSGMRLDA

Sequences:

>Translated_548_residues
MAVVPLITPPVLIVGAGPTGLAAALCLARARVPVRIVDKAARPAHHSRAIGIQARTLELLEQQRVVDRFVELGHRARLAI
LYSAGQRIAELDFDPLQTRYPYLLFLDQTVTERLLTEHLATFDVHVERGVTLTHCTEPEGALDVRLRHGDGRDEHLQPSY
VVAADGAHSTVRRLLDVDFVGHAFEQTFLLADLDVGADWPDDEIHLFTTGDGIAGLFPMGNGRYRLVADRPPRNGALDDE
HGPSLELCREIVRARVTPNLKIGDLAWSSYFHLHSRMVAKLRVGRVFFAGDAAHVHSPAGAQGMNTGIQEAFNLGWKLAR
VLAGNAPERLLDTYHEERHPIERDVLRQTSFATQVVEADRGPLRLLRNHVVPILASFGPLRDAARRSVSELAIQYRKSPL
TLERVLDGGPRAGERAPDALLHVLDGPLGKAPGVARLFDLHDPAHFTLLVLEPREAIDDTLPSDPGEDGRALADALERIM
PGAVRCWRVTDAQGEGAPLLIDAYGRSRPVFYLLRPDGYVAARGRPATDANALLRHCETWFSGMRLDA
>Mature_547_residues
AVVPLITPPVLIVGAGPTGLAAALCLARARVPVRIVDKAARPAHHSRAIGIQARTLELLEQQRVVDRFVELGHRARLAIL
YSAGQRIAELDFDPLQTRYPYLLFLDQTVTERLLTEHLATFDVHVERGVTLTHCTEPEGALDVRLRHGDGRDEHLQPSYV
VAADGAHSTVRRLLDVDFVGHAFEQTFLLADLDVGADWPDDEIHLFTTGDGIAGLFPMGNGRYRLVADRPPRNGALDDEH
GPSLELCREIVRARVTPNLKIGDLAWSSYFHLHSRMVAKLRVGRVFFAGDAAHVHSPAGAQGMNTGIQEAFNLGWKLARV
LAGNAPERLLDTYHEERHPIERDVLRQTSFATQVVEADRGPLRLLRNHVVPILASFGPLRDAARRSVSELAIQYRKSPLT
LERVLDGGPRAGERAPDALLHVLDGPLGKAPGVARLFDLHDPAHFTLLVLEPREAIDDTLPSDPGEDGRALADALERIMP
GAVRCWRVTDAQGEGAPLLIDAYGRSRPVFYLLRPDGYVAARGRPATDANALLRHCETWFSGMRLDA

Specific function: Dechlorination of pentachlorophenol to tetrachlorohydroquinone. Removes also hydrogen and nitro, amino, and cyano groups from benzene ring at the para position in relation to the hydroxyl of phenol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=384, Percent_Identity=26.3020833333333, Blast_Score=113, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: =1.14.13.50 [H]

Molecular weight: Translated: 60324; Mature: 60192

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVVPLITPPVLIVGAGPTGLAAALCLARARVPVRIVDKAARPAHHSRAIGIQARTLELL
CEECCCCCCCEEEEECCCHHHHHHHHHHHCCCCEEEHHHCCCCCCCCCEECCHHHHHHHH
EQQRVVDRFVELGHRARLAILYSAGQRIAELDFDPLQTRYPYLLFLDQTVTERLLTEHLA
HHHHHHHHHHHCCCCEEEEEEECCCCHHHHCCCCCCCCCCCEEEEECHHHHHHHHHHHHH
TFDVHVERGVTLTHCTEPEGALDVRLRHGDGRDEHLQPSYVVAADGAHSTVRRLLDVDFV
HHEEEEECCCEEEECCCCCCCEEEEEECCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHH
GHAFEQTFLLADLDVGADWPDDEIHLFTTGDGIAGLFPMGNGRYRLVADRPPRNGALDDE
HHHHHHEEEEEEECCCCCCCCCCEEEEECCCCCEEEEECCCCCEEEEECCCCCCCCCCCC
HGPSLELCREIVRARVTPNLKIGDLAWSSYFHLHSRMVAKLRVGRVFFAGDAAHVHSPAG
CCCCHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHCEEEEECCHHHCCCCCC
AQGMNTGIQEAFNLGWKLARVLAGNAPERLLDTYHEERHPIERDVLRQTSFATQVVEADR
CCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC
GPLRLLRNHVVPILASFGPLRDAARRSVSELAIQYRKSPLTLERVLDGGPRAGERAPDAL
CHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCHHHH
LHVLDGPLGKAPGVARLFDLHDPAHFTLLVLEPREAIDDTLPSDPGEDGRALADALERIM
HHHHCCCCCCCCCCEEEEECCCCCEEEEEEECCHHHHHHCCCCCCCCCHHHHHHHHHHHC
PGAVRCWRVTDAQGEGAPLLIDAYGRSRPVFYLLRPDGYVAARGRPATDANALLRHCETW
CCCEEEEEEECCCCCCCCEEEECCCCCCCEEEEECCCCEEEECCCCCCCHHHHHHHHHHH
FSGMRLDA
HCCCCCCC
>Mature Secondary Structure 
AVVPLITPPVLIVGAGPTGLAAALCLARARVPVRIVDKAARPAHHSRAIGIQARTLELL
EECCCCCCCEEEEECCCHHHHHHHHHHHCCCCEEEHHHCCCCCCCCCEECCHHHHHHHH
EQQRVVDRFVELGHRARLAILYSAGQRIAELDFDPLQTRYPYLLFLDQTVTERLLTEHLA
HHHHHHHHHHHCCCCEEEEEEECCCCHHHHCCCCCCCCCCCEEEEECHHHHHHHHHHHHH
TFDVHVERGVTLTHCTEPEGALDVRLRHGDGRDEHLQPSYVVAADGAHSTVRRLLDVDFV
HHEEEEECCCEEEECCCCCCCEEEEEECCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHH
GHAFEQTFLLADLDVGADWPDDEIHLFTTGDGIAGLFPMGNGRYRLVADRPPRNGALDDE
HHHHHHEEEEEEECCCCCCCCCCEEEEECCCCCEEEEECCCCCEEEEECCCCCCCCCCCC
HGPSLELCREIVRARVTPNLKIGDLAWSSYFHLHSRMVAKLRVGRVFFAGDAAHVHSPAG
CCCCHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHCEEEEECCHHHCCCCCC
AQGMNTGIQEAFNLGWKLARVLAGNAPERLLDTYHEERHPIERDVLRQTSFATQVVEADR
CCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC
GPLRLLRNHVVPILASFGPLRDAARRSVSELAIQYRKSPLTLERVLDGGPRAGERAPDAL
CHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCHHHH
LHVLDGPLGKAPGVARLFDLHDPAHFTLLVLEPREAIDDTLPSDPGEDGRALADALERIM
HHHHCCCCCCCCCCEEEEECCCCCEEEEEEECCHHHHHHCCCCCCCCCHHHHHHHHHHHC
PGAVRCWRVTDAQGEGAPLLIDAYGRSRPVFYLLRPDGYVAARGRPATDANALLRHCETW
CCCEEEEEEECCCCCCCCEEEECCCCCCCEEEEECCCCEEEECCCCCCCHHHHHHHHHHH
FSGMRLDA
HCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7678243; 12169590 [H]