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The map label for this gene is citH [H]

Identifier: 83718592

GI number: 83718592

Start: 1821333

End: 1822628

Strand: Direct

Name: citH [H]

Synonym: BTH_I1618

Alternate gene names: 83718592

Gene position: 1821333-1822628 (Clockwise)

Preceding gene: 83721310

Following gene: 83720382

Centisome position: 47.81

GC content: 65.51

Gene sequence:

>1296_bases
ATGTCTACCGCGTCCCCCGCAATTCAGCAGGAATCCAAGGCCAGGACCGTGTTCCGCGTCGTCAGCGGCAACTTCCTGGA
GATGTACGACTTCATGGTCTACGGCTACTACGCGTCCGCGATCGCCAAGACCTACTTCCCGAGCGGCAACGCGTTTGCGT
CGCTGATGCTGTCACTGTCCGTGTTCGGCGCGGGCTTCCTGATGCGCCCCGTCGGCGCGATCGTGCTCGGCGCGTACATC
GATCATCATGGCCGCCGCAAGGGCCTCATCCTGACGCTCGCGCTGATGGCGCTCGGCACGCTGACGGTCGCCACGATTCC
GGGCTATGCGACGATCGGCGTGCTCGCGCCGATTCTCGTGCTGCTCGGCCGCCTGCTGCAAGGTTTCTCGGCGGGTGTCG
AGCTGGGCGGCGTGTCGGTGTACCTGTCCGAGATCGCGACGAAGGGTAACAAGGGCTTCTACACGTCGTGGCAGTCGGGC
AGCCAGCAGGTGGCGGTCGTGTTCGCCGCGTTCGTCGGCGTGCTGCTGAACCGCGCGCTGCCCGTCGAGCAGATGACGTC
GTGGGGCTGGCGCATTCCGTTCCTGATCGGCTGCCTGATCGTGCCGTTCCTGTTCCTGATCCGCCGCTCGCTGAAGGAGA
CCGACGAGTTTCTCGCGAAGCGCCACCGTCCGAGCATGGGCGAGATCATGAAGTCGATGCTCGAAAACTGGGGCGTCGTG
CTCGCCGGCATGGGGATGGTCATCATGACGACCGTGTCGTTCTACATGATCACCGCGTACACGCCGACTTTCGGCAAGGA
AGTGCTGCACCTGTCGGCGATCGACGCGCTCGTCGTGACCGTCTGTGTCGGGCTGTCGAACCTGATCTGGCTGCCGCTGT
CGGGCGCGCTGTCCGACCGGATCGGCCGGCGCCCCGTGCTGATCGCGTTCACGGCGCTCACGATCCTCACCGCGTATCCG
GCGATGCAGTGGCTTGTCGGTTCGCCGTCGTTCCTGCGGCTGCTGACCGTCGAACTGTGGCTGTCGTTCCTGTACGGCTC
GTACAACGGCGCGATGGTCGTCGCGCTGACCGAGGTGATGCCGGCAGACGTGCGCACCGCAGGCTTCTCGCTCGCGTACA
GCCTCGCGACGACGATCGGCGGCTTCACGCCGGCGATCTCGACGCTGCTGATCCACGAGAGCGGCAACAAGGCGGCGCCG
GGGCTCTGGCTCGGCCTTGCCGCGATCTGCGGACTGATCGCGACGCTCGTGCTGTACCGGTCGCCGCAAGCGCGCAATCA
GTACAAGACGACTTGA

Upstream 100 bases:

>100_bases
GATCTGCGCGGCCCCCGGCCGCGCCGGGGAAAGAAGCACGATGTGATACGCGCCGCGCGCCGCCTGCCGGTGGCCGCGGC
GCAGCCTTATCGAGAGAGAC

Downstream 100 bases:

>100_bases
GCGGACGGGCGGCCTGCTGCGAACGGGCCGTCGACGGCGAATCGGGAAACGGAGCGCGCCGCGCTCCGTTTTTTGTTTGA
GCGAAGCGTTGGCGCGGCGC

Product: citrate-proten symporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MSTASPAIQQESKARTVFRVVSGNFLEMYDFMVYGYYASAIAKTYFPSGNAFASLMLSLSVFGAGFLMRPVGAIVLGAYI
DHHGRRKGLILTLALMALGTLTVATIPGYATIGVLAPILVLLGRLLQGFSAGVELGGVSVYLSEIATKGNKGFYTSWQSG
SQQVAVVFAAFVGVLLNRALPVEQMTSWGWRIPFLIGCLIVPFLFLIRRSLKETDEFLAKRHRPSMGEIMKSMLENWGVV
LAGMGMVIMTTVSFYMITAYTPTFGKEVLHLSAIDALVVTVCVGLSNLIWLPLSGALSDRIGRRPVLIAFTALTILTAYP
AMQWLVGSPSFLRLLTVELWLSFLYGSYNGAMVVALTEVMPADVRTAGFSLAYSLATTIGGFTPAISTLLIHESGNKAAP
GLWLGLAAICGLIATLVLYRSPQARNQYKTT

Sequences:

>Translated_431_residues
MSTASPAIQQESKARTVFRVVSGNFLEMYDFMVYGYYASAIAKTYFPSGNAFASLMLSLSVFGAGFLMRPVGAIVLGAYI
DHHGRRKGLILTLALMALGTLTVATIPGYATIGVLAPILVLLGRLLQGFSAGVELGGVSVYLSEIATKGNKGFYTSWQSG
SQQVAVVFAAFVGVLLNRALPVEQMTSWGWRIPFLIGCLIVPFLFLIRRSLKETDEFLAKRHRPSMGEIMKSMLENWGVV
LAGMGMVIMTTVSFYMITAYTPTFGKEVLHLSAIDALVVTVCVGLSNLIWLPLSGALSDRIGRRPVLIAFTALTILTAYP
AMQWLVGSPSFLRLLTVELWLSFLYGSYNGAMVVALTEVMPADVRTAGFSLAYSLATTIGGFTPAISTLLIHESGNKAAP
GLWLGLAAICGLIATLVLYRSPQARNQYKTT
>Mature_430_residues
STASPAIQQESKARTVFRVVSGNFLEMYDFMVYGYYASAIAKTYFPSGNAFASLMLSLSVFGAGFLMRPVGAIVLGAYID
HHGRRKGLILTLALMALGTLTVATIPGYATIGVLAPILVLLGRLLQGFSAGVELGGVSVYLSEIATKGNKGFYTSWQSGS
QQVAVVFAAFVGVLLNRALPVEQMTSWGWRIPFLIGCLIVPFLFLIRRSLKETDEFLAKRHRPSMGEIMKSMLENWGVVL
AGMGMVIMTTVSFYMITAYTPTFGKEVLHLSAIDALVVTVCVGLSNLIWLPLSGALSDRIGRRPVLIAFTALTILTAYPA
MQWLVGSPSFLRLLTVELWLSFLYGSYNGAMVVALTEVMPADVRTAGFSLAYSLATTIGGFTPAISTLLIHESGNKAAPG
LWLGLAAICGLIATLVLYRSPQARNQYKTT

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=421, Percent_Identity=31.353919239905, Blast_Score=211, Evalue=5e-56,
Organism=Escherichia coli, GI1788942, Length=431, Percent_Identity=33.1786542923434, Blast_Score=192, Evalue=4e-50,
Organism=Escherichia coli, GI1788292, Length=415, Percent_Identity=28.433734939759, Blast_Score=169, Evalue=5e-43,
Organism=Escherichia coli, GI1789941, Length=425, Percent_Identity=30.8235294117647, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI87082231, Length=162, Percent_Identity=29.6296296296296, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46494; Mature: 46362

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCCC
>Mature Secondary Structure 
STASPAIQQESKARTVFRVVSGNFLEMYDFMVYGYYASAIAKTYFPSGNAFASLMLSLS
CCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
VFGAGFLMRPVGAIVLGAYIDHHGRRKGLILTLALMALGTLTVATIPGYATIGVLAPILV
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LLGRLLQGFSAGVELGGVSVYLSEIATKGNKGFYTSWQSGSQQVAVVFAAFVGVLLNRAL
HHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHCCC
PVEQMTSWGWRIPFLIGCLIVPFLFLIRRSLKETDEFLAKRHRPSMGEIMKSMLENWGVV
CHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHH
LAGMGMVIMTTVSFYMITAYTPTFGKEVLHLSAIDALVVTVCVGLSNLIWLPLSGALSDR
HHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHH
IGRRPVLIAFTALTILTAYPAMQWLVGSPSFLRLLTVELWLSFLYGSYNGAMVVALTEVM
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHC
PADVRTAGFSLAYSLATTIGGFTPAISTLLIHESGNKAAPGLWLGLAAICGLIATLVLYR
CCHHHHHHHHHHHHHHHHHCCHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHC
SPQARNQYKTT
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]