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The map label for this gene is nolF [H]

Identifier: 83720332

GI number: 83720332

Start: 2579910

End: 2581025

Strand: Reverse

Name: nolF [H]

Synonym: BTH_I2289

Alternate gene names: 83720332

Gene position: 2581025-2579910 (Counterclockwise)

Preceding gene: 83721209

Following gene: 83721121

Centisome position: 67.76

GC content: 73.12

Gene sequence:

>1116_bases
GTGAATCGTTCCGGTTCTCGCGCCGCGCTGCTGATCGGCGTCGCGCTCATCGTTGCCGCCTGTCACCGCAAGGAAGCCGC
GCCGAGCGCGCCGCGCCCCGTCGTCGCGGTGCCGGTCCGGGCGGACGGCGCAGCCGCCGCCGTGTCGCTGCCCGGCGAGA
TCCAGCCGCGCTATGCGACGCCGCTGTCGTTCCGCATCGCCGGCAAGATCGTCGAGCGCAAGGTCCGCCTCGGCGACGCC
GTGAAGAAAGGCCAGGTTCTCGCGCTGCTCGACACATCCGACGTCGCGAAGAACGCGGCGAGCGCGCAGGCGCAGCTCGA
CGCCGCGACGCACGCGCTGACGTTCGCGCAACAGCAGCGCGAGCGCGACCGCGCGCAGGCGCGCGAGAACCTGATCGCGC
CCGCGCAGCTCGAGCAGACCGAGAACGCCTACGCGGCCGCGCGCGCGCAGCGCGACCAGGCCGAGCAGCAGCTCGCGCTC
GCGAAGAACCAGTTGCAGTACGCGACGCTCGTCGCCGACCATGCGGGCTACATCACCGCCGAGCAGGCCGATACCGGGCA
GAACGTGTCCGCCGGCCAGCCGGTCTATCAGCTCGCGTGGTCGGGTGATGTCGACGTCGTCACCGACGCGCCGGAAAGCG
AGCTGCCGCTCCTCGTGCCGGGCCATGCGGCGCGCGTCACGCTGCCGGCGCTGCCCGGCCGCGCGTTCGACGCGCGCGTG
CGCGAGATCGCGCCCGCCGCCGATCCGCAAAGCCGCACGTACCGCGTGAAGCTCACGCTCGCGCAGCCCGACTCCGCCGT
GCGGCTCGGGATGACCGCGACCGTCGCGTTCTCGTCGGGCGCGCCGGGCAACGCGAGCGCGCAGGCGCGCTTCACGCTGC
CGTCGACGGCGCTCTTCCACGCAGGCAACGCGCCCGCGCTGTGGGTCGTGCGCAAGCAGGACGACACGCTCGAGCTGCGG
CGCGTCGACATCGTCCGCTACAACGAGCGCACGGTGACCGTGTCGGGCGGAATTCAGCCGGGCGAGCGCGTCGTGCTGCA
AGGCGTGCACACGGTGAGCGCGGGCGAGAAGGTGCGCGCGGTGCCGCCGCTTCATCCGGAGGACCTCGCGTCATGA

Upstream 100 bases:

>100_bases
CAACGTGCGCGCCGATTGTTTTGACTCTTGCCGCGCGCCGCGCCAGGCAAGCGCATCCGGCTTGCCGGTTTTTCCGTTTT
CTCCTGTTCGAGGGGTCACC

Downstream 100 bases:

>100_bases
CGGCAGGCCGAGAAGAAGGCGGACGCTTCAACCTGTCCGCGTGGGCGCTGCGCCACCAGGCGCTCGTCATCTACCTGATC
GCGCTCTCGACGATCGCCGG

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MNRSGSRAALLIGVALIVAACHRKEAAPSAPRPVVAVPVRADGAAAAVSLPGEIQPRYATPLSFRIAGKIVERKVRLGDA
VKKGQVLALLDTSDVAKNAASAQAQLDAATHALTFAQQQRERDRAQARENLIAPAQLEQTENAYAAARAQRDQAEQQLAL
AKNQLQYATLVADHAGYITAEQADTGQNVSAGQPVYQLAWSGDVDVVTDAPESELPLLVPGHAARVTLPALPGRAFDARV
REIAPAADPQSRTYRVKLTLAQPDSAVRLGMTATVAFSSGAPGNASAQARFTLPSTALFHAGNAPALWVVRKQDDTLELR
RVDIVRYNERTVTVSGGIQPGERVVLQGVHTVSAGEKVRAVPPLHPEDLAS

Sequences:

>Translated_371_residues
MNRSGSRAALLIGVALIVAACHRKEAAPSAPRPVVAVPVRADGAAAAVSLPGEIQPRYATPLSFRIAGKIVERKVRLGDA
VKKGQVLALLDTSDVAKNAASAQAQLDAATHALTFAQQQRERDRAQARENLIAPAQLEQTENAYAAARAQRDQAEQQLAL
AKNQLQYATLVADHAGYITAEQADTGQNVSAGQPVYQLAWSGDVDVVTDAPESELPLLVPGHAARVTLPALPGRAFDARV
REIAPAADPQSRTYRVKLTLAQPDSAVRLGMTATVAFSSGAPGNASAQARFTLPSTALFHAGNAPALWVVRKQDDTLELR
RVDIVRYNERTVTVSGGIQPGERVVLQGVHTVSAGEKVRAVPPLHPEDLAS
>Mature_371_residues
MNRSGSRAALLIGVALIVAACHRKEAAPSAPRPVVAVPVRADGAAAAVSLPGEIQPRYATPLSFRIAGKIVERKVRLGDA
VKKGQVLALLDTSDVAKNAASAQAQLDAATHALTFAQQQRERDRAQARENLIAPAQLEQTENAYAAARAQRDQAEQQLAL
AKNQLQYATLVADHAGYITAEQADTGQNVSAGQPVYQLAWSGDVDVVTDAPESELPLLVPGHAARVTLPALPGRAFDARV
REIAPAADPQSRTYRVKLTLAQPDSAVRLGMTATVAFSSGAPGNASAQARFTLPSTALFHAGNAPALWVVRKQDDTLELR
RVDIVRYNERTVTVSGGIQPGERVVLQGVHTVSAGEKVRAVPPLHPEDLAS

Specific function: Involved in the production of Medicago-specific nodulation signal molecule [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Attached To The Inner Membrane By A Lipid Anchor [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87081796, Length=369, Percent_Identity=23.8482384823848, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 39235; Mature: 39235

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRSGSRAALLIGVALIVAACHRKEAAPSAPRPVVAVPVRADGAAAAVSLPGEIQPRYAT
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCEEEEECCCCCCCCCCC
PLSFRIAGKIVERKVRLGDAVKKGQVLALLDTSDVAKNAASAQAQLDAATHALTFAQQQR
CEEEEHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERDRAQARENLIAPAQLEQTENAYAAARAQRDQAEQQLALAKNQLQYATLVADHAGYITA
HHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEE
EQADTGQNVSAGQPVYQLAWSGDVDVVTDAPESELPLLVPGHAARVTLPALPGRAFDARV
CCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCCCCEEECCCCCEEEECCCCCCHHHHHH
REIAPAADPQSRTYRVKLTLAQPDSAVRLGMTATVAFSSGAPGNASAQARFTLPSTALFH
HHHCCCCCCCCCEEEEEEEEECCCCEEEECEEEEEEECCCCCCCCCCEEEEECCCCEEEE
AGNAPALWVVRKQDDTLELRRVDIVRYNERTVTVSGGIQPGERVVLQGVHTVSAGEKVRA
CCCCCEEEEEECCCCCEEEEEEEEEEECCEEEEEECCCCCCCCEEEEECEEECCCCCEEC
VPPLHPEDLAS
CCCCCHHHCCC
>Mature Secondary Structure
MNRSGSRAALLIGVALIVAACHRKEAAPSAPRPVVAVPVRADGAAAAVSLPGEIQPRYAT
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCEEEEECCCCCCCCCCC
PLSFRIAGKIVERKVRLGDAVKKGQVLALLDTSDVAKNAASAQAQLDAATHALTFAQQQR
CEEEEHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERDRAQARENLIAPAQLEQTENAYAAARAQRDQAEQQLALAKNQLQYATLVADHAGYITA
HHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEE
EQADTGQNVSAGQPVYQLAWSGDVDVVTDAPESELPLLVPGHAARVTLPALPGRAFDARV
CCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCCCCEEECCCCCEEEECCCCCCHHHHHH
REIAPAADPQSRTYRVKLTLAQPDSAVRLGMTATVAFSSGAPGNASAQARFTLPSTALFH
HHHCCCCCCCCCEEEEEEEEECCCCEEEECEEEEEEECCCCCCCCCCEEEEECCCCEEEE
AGNAPALWVVRKQDDTLELRRVDIVRYNERTVTVSGGIQPGERVVLQGVHTVSAGEKVRA
CCCCCEEEEEECCCCCEEEEEEEEEEECCEEEEEECCCCCCCCEEEEECEEECCCCCEEC
VPPLHPEDLAS
CCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1909418; 11481432 [H]