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The map label for this gene is glcF [H]

Identifier: 83720279

GI number: 83720279

Start: 1440548

End: 1441774

Strand: Reverse

Name: glcF [H]

Synonym: BTH_I1289

Alternate gene names: 83720279

Gene position: 1441774-1440548 (Counterclockwise)

Preceding gene: 83721183

Following gene: 83721068

Centisome position: 37.85

GC content: 69.76

Gene sequence:

>1227_bases
ATGCAGACGAACCTCGCCGATTTCATCCGCCACACGCCCGACGGCGACGACGCCGACGCGATCCTGCGCAAGTGCGTGCA
CTGCGGCTTCTGCACCGCGACCTGCCCGACCTATCAATTGCTCGGCGACGAACTCGACGGCCCGCGCGGACGCATCTACC
TGATCAAGCAGATGCTCGAGGGCGCGGAGGTCACGCGCAGCACGCAGCAGCATCTCGACCGCTGCCTCACATGCCGCAGT
TGCGAGACGACCTGCCCGTCGGGCGTCGAGTACGGCCGGCTCGTCGACATCGGCCGCAAGGTCGTCGAGACGAAGGTGTC
GCGGCCGCTCGCGCAGCGGATCGTGCGGCGCCTGCTCGCGAGCTTCGTGCCGAACGCCTCCGTGTTCGCGCCCGTGATGC
GGCTCGGCCAGCACGTGCGGCCGCTGCTGCCGAAGCGGCTGCGCGACAAGGTGCCGCCGCGCGCGCGCCTGCTCGCGTGG
CCGACCGCGCGGCGCGAGCGCCGCGTGCTGATGCTCGCGGGCTGCGTGCAGCCGTCGATGATGCCGAACATCAACATCGC
AACCGCGCGCGTGCTGGATGCGCTCGGCATCGAGACCGTGGTCGCGCCGGAAGCCGGCTGCTGCGGCGCGATCCGGCTGC
ATCTCGGCTATCACGACGAAGCGCTCGACGACGCGCGCCGCAACATCGACGCGTGGTGGCCGCACGTCGAGCGCGGCGTC
GACGCGATCGTGATGAACGCGTCCGGCTGCGGCGCGACGGTGCGCGAGTACGCGCACCTGCTGCGCGGCGACGCGGCGTA
CGCGGACAAGGCGCGCCGCATCGTCGAGCTGACGAAGGACGCATCCGAGCTGCTCGGCGGCTTCGAGGCCGAGCTGATCG
CGCTCACGCGCCGGCGCGGCATCCACACGGTCGCCTATCATCCGCCGTGCACGCTACAGCATGGCCAGCAGTTGCGCGGC
AAGGTCGAGCGGCTGCTGGAGGCGCTCGGCATCGAAGTGCGGCTGCCCGCCGACAGCCATCTGTGCTGCGGGTCCGCAGG
CACCTACTCGCTCACGCAGCCGTCGCTCGCATACAAGCTGCGCAAGCAGAAACTCGCGAAGCTGCAGGCGACCGAGCCGC
AAATGATCGTATCGGCGAACATCGGCTGCATCGCGCATCTGCAAAGCGGCACGCCGGTGCCCGTCGTGCACTGGGTGCAG
CTCGTCGAGAATCTGCTGTACGGTTGA

Upstream 100 bases:

>100_bases
GCCCGCGCCGCTGATGAAAATCCACCGCGGCCTGAAGACGGCGTTCGATCCGGCGCGCATCTTCAACCGCGGCCGGCTCT
ACCCCGATCTCTAAAGTGCG

Downstream 100 bases:

>100_bases
CGGCGCGCGACGGGCGGCGCTTCGCGCCCGTCCGTCGTTCGCGGCGATCATCCGCGCCGGTTGTCAGCGCCGGTTGTCCG
TATAATTCCCGCATCCGTCG

Product: glycolate oxidase iron-sulfur subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MQTNLADFIRHTPDGDDADAILRKCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQMLEGAEVTRSTQQHLDRCLTCRS
CETTCPSGVEYGRLVDIGRKVVETKVSRPLAQRIVRRLLASFVPNASVFAPVMRLGQHVRPLLPKRLRDKVPPRARLLAW
PTARRERRVLMLAGCVQPSMMPNINIATARVLDALGIETVVAPEAGCCGAIRLHLGYHDEALDDARRNIDAWWPHVERGV
DAIVMNASGCGATVREYAHLLRGDAAYADKARRIVELTKDASELLGGFEAELIALTRRRGIHTVAYHPPCTLQHGQQLRG
KVERLLEALGIEVRLPADSHLCCGSAGTYSLTQPSLAYKLRKQKLAKLQATEPQMIVSANIGCIAHLQSGTPVPVVHWVQ
LVENLLYG

Sequences:

>Translated_408_residues
MQTNLADFIRHTPDGDDADAILRKCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQMLEGAEVTRSTQQHLDRCLTCRS
CETTCPSGVEYGRLVDIGRKVVETKVSRPLAQRIVRRLLASFVPNASVFAPVMRLGQHVRPLLPKRLRDKVPPRARLLAW
PTARRERRVLMLAGCVQPSMMPNINIATARVLDALGIETVVAPEAGCCGAIRLHLGYHDEALDDARRNIDAWWPHVERGV
DAIVMNASGCGATVREYAHLLRGDAAYADKARRIVELTKDASELLGGFEAELIALTRRRGIHTVAYHPPCTLQHGQQLRG
KVERLLEALGIEVRLPADSHLCCGSAGTYSLTQPSLAYKLRKQKLAKLQATEPQMIVSANIGCIAHLQSGTPVPVVHWVQ
LVENLLYG
>Mature_408_residues
MQTNLADFIRHTPDGDDADAILRKCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQMLEGAEVTRSTQQHLDRCLTCRS
CETTCPSGVEYGRLVDIGRKVVETKVSRPLAQRIVRRLLASFVPNASVFAPVMRLGQHVRPLLPKRLRDKVPPRARLLAW
PTARRERRVLMLAGCVQPSMMPNINIATARVLDALGIETVVAPEAGCCGAIRLHLGYHDEALDDARRNIDAWWPHVERGV
DAIVMNASGCGATVREYAHLLRGDAAYADKARRIVELTKDASELLGGFEAELIALTRRRGIHTVAYHPPCTLQHGQQLRG
KVERLLEALGIEVRLPADSHLCCGSAGTYSLTQPSLAYKLRKQKLAKLQATEPQMIVSANIGCIAHLQSGTPVPVVHWVQ
LVENLLYG

Specific function: Unknown

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI48994913, Length=407, Percent_Identity=56.5110565110565, Blast_Score=462, Evalue=1e-131,
Organism=Escherichia coli, GI1788576, Length=355, Percent_Identity=24.7887323943662, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1786497, Length=241, Percent_Identity=22.4066390041494, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: NA

Molecular weight: Translated: 45042; Mature: 45042

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
3.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTNLADFIRHTPDGDDADAILRKCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQMLE
CCCCHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHH
GAEVTRSTQQHLDRCLTCRSCETTCPSGVEYGRLVDIGRKVVETKVSRPLAQRIVRRLLA
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
SFVPNASVFAPVMRLGQHVRPLLPKRLRDKVPPRARLLAWPTARRERRVLMLAGCVQPSM
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHCEEEEEECCCCCCC
MPNINIATARVLDALGIETVVAPEAGCCGAIRLHLGYHDEALDDARRNIDAWWPHVERGV
CCCCCHHHHHHHHHHCCHHEECCCCCCCEEEEEEECCCHHHHHHHHHCCHHCCHHHHCCC
DAIVMNASGCGATVREYAHLLRGDAAYADKARRIVELTKDASELLGGFEAELIALTRRRG
CEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCC
IHTVAYHPPCTLQHGQQLRGKVERLLEALGIEVRLPADSHLCCGSAGTYSLTQPSLAYKL
CEEEEECCCCCHHHHHHHHHHHHHHHHHHCCEEECCCCCCEECCCCCCCCCCCHHHHHHH
RKQKLAKLQATEPQMIVSANIGCIAHLQSGTPVPVVHWVQLVENLLYG
HHHHHHHHHCCCCCEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQTNLADFIRHTPDGDDADAILRKCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQMLE
CCCCHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHH
GAEVTRSTQQHLDRCLTCRSCETTCPSGVEYGRLVDIGRKVVETKVSRPLAQRIVRRLLA
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
SFVPNASVFAPVMRLGQHVRPLLPKRLRDKVPPRARLLAWPTARRERRVLMLAGCVQPSM
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHCEEEEEECCCCCCC
MPNINIATARVLDALGIETVVAPEAGCCGAIRLHLGYHDEALDDARRNIDAWWPHVERGV
CCCCCHHHHHHHHHHCCHHEECCCCCCCEEEEEEECCCHHHHHHHHHCCHHCCHHHHCCC
DAIVMNASGCGATVREYAHLLRGDAAYADKARRIVELTKDASELLGGFEAELIALTRRRG
CEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCC
IHTVAYHPPCTLQHGQQLRGKVERLLEALGIEVRLPADSHLCCGSAGTYSLTQPSLAYKL
CEEEEECCCCCHHHHHHHHHHHHHHHHHHCCEEECCCCCCEECCCCCCCCCCCHHHHHHH
RKQKLAKLQATEPQMIVSANIGCIAHLQSGTPVPVVHWVQLVENLLYG
HHHHHHHHHCCCCCEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8606183; 9278503 [H]