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The map label for this gene is pnp [H]

Identifier: 83718859

GI number: 83718859

Start: 1198682

End: 1200823

Strand: Direct

Name: pnp [H]

Synonym: BTH_I1056

Alternate gene names: 83718859

Gene position: 1198682-1200823 (Clockwise)

Preceding gene: 161723163

Following gene: 83720037

Centisome position: 31.47

GC content: 65.69

Gene sequence:

>2142_bases
ATGTCTCTGTTCAATAAAATCGTGAAGGAATTCCAGTGGGGCCAGCACAAGGTGCGCCTCGAAACCGGCGAAATCGCGCG
CCAGGCTAGCGGCGCCGTCCTCGTCGACGTCGAGGACACCGTCGTGCTCGCGACCGTCGTCGGCGCGAAGTCGGCGAAGC
CGGGGCAGGATTTCTTCCCGCTCACTGTCGACTACATCGAGAAGACCTACTCGGCCGGCAAGATCCCGGGCGGTTTCTTC
CGCCGCGAAGGCCGTCCGTCCGAGCACGAAACGCTGACGTCGCGCCTCATCGACCGGCCGCTGCGCCCGCTCTTCCCGGA
AGGTTTCTATAACGAAGTGCAGGTCGTGATTCACGTGCTGTCGGTGAACCCGGAAGTCCCGGCGGACATCCCGGCGCTCA
TCGGCGCATCGGCTGCGCTCGCCGTGTCCGGCCTGCCGTTCAACGGCCCGGTCGGCGCCGCGCGCGTCGCGTACGTGAAC
AATGAATACGTGCTGAACCCGACGCGCGAGCAGATCAAGGCGTCGCGCCTCGACCTCGTCGTCGCGGGCACGGAGCGCGC
GGTGCTGATGGTCGAGTCCGAGGCCGATCAGTTGCCGGAAGACGTGATGCTCGGCGCGGTCGTGTTCGGCCATGAGCAGA
TGCAGACCGCGATCGACGCGATCCACGAACTCGTGCGCGAAGGCGGCAAGCCCGAGTGGGACTGGCAGCCGGCGCCGAAG
GACGAGGCGCTGAACGCACGCGTGACCGAGCTCGCGCAGCCGGAACTGCTGGCGGCCTACCAGATCCGCGACAAGCAGGC
GCGCTCGACGAAGCTGAAGGAAGTGTACGCGGCAACCTCGGCGAAGCTGGAGGAAGAGGCGCTGGCGGCCGGCACGGTCG
CGGCCGACAAGGCAACCGTCGGCAACATCCTGTTCGACCTCGAGGCAAAGATCGTCCGCGGCCAGATCCTGAGCGGCGAG
CCGCGCATCGACGGCCGCGACACCCGCACCGTGCGCCCGATCGAGATCCGCACGGGCGTGCTGCCGCGCACCCACGGCTC
GGCGCTGTTCACGCGCGGCGAGACACAAGCGCTGGTGGTCGCGACGCTCGGCACGAAGGGCGATGAGCAGATCATCGACG
CGCTCGAAGGCGAGTACCGCGACCGCTTCATGCTCCACTACAACATGCCCCCGTTCGCGACCGGCGAAACGGGCCGCGTC
GGCTCGCCGAAGCGTCGCGAAATCGGCCACGGCCGGCTCGCGAAGCGCGCGCTCGTCGCGTGCCTGCCGAGCGCCGACGA
ATTCGGCTACTCGATCCGCGTCGTGTCGGAAATCACCGAGTCGAACGGTTCGTCGTCGATGGCGTCGGTGTGCGGCGGCT
GCCTCGCGCTGATGGACGCCGGCGTGCCGATGAAGGCGCACGTCGCGGGCATCGCGATGGGCCTCATCCTCGAGGGCAAC
AAGTTCGCGGTGTTGACCGACATCCTCGGCGACGAAGATCACCTCGGCGACATGGACTTCAAGGTGGCCGGCACGGCGGA
AGGCGTGACGGCGCTGCAGATGGACATCAAGATCCAGGGCATCACGAAGGAAATCATGCAGGTCGCGCTCGCGCAGGCGA
AGGAAGGCCGCATGCACATCCTCGGCAAGATGAAGGAAGCGGTCGCGGGTGCGAACACGCAGCTGTCCGAATTCGCGCCG
CGCATGATCACGATCAAGATCAACCCGGAAAAGATCCGTGACGTGATCGGCAAGGGCGGTTCGGTGATCCGCGCGCTGAC
GGAAGAAACCGGCACGACGATCGACATCTCCGACGACGGCGTCGTGACGATTGCGAGCACGAACAGCGAAGGCATGGCCG
AAGCGAAGAAGCGCATCGAGAACATCACGGCCGAGATCGAAGTCGGTCAGGTGTACGAAGGCACGGTGTTGAAGCTCCTC
GATTTCGGCGCGATCGTGAACCTGCTGCCGGGCAAGGACGGTCTGCTGCACATCTCCGAGATCGTCAACGAGCGCGTGAA
GGACATCAACGACTACCTGAAGGAAGGTCAGCAGGTGAAGGTCAAGGTGATCCAGACGGACGAGAAGGGGCGCGTGCGTT
TGTCGGCGAAGGCGCTGCTGAACGAGGCGGCTGGGCAGACGGATACGCCGCCGCAGCAGTAA

Upstream 100 bases:

>100_bases
GGCATAAAAAACACACCTCTTCCCATGTGGCCCGGTCGCGCCGCCGCCGTGCCGGCCTCGTCCGCGCACGGCGTCCGACA
ACGAACGAAAAGGAGCGACC

Downstream 100 bases:

>100_bases
GCGGCAAGGCAACGGCCGGCAGCGCGAACGCGCGCCGGCCGTTTTTCACAGGATGGGCCGGTTTGCGGGGCGCGCATCGC
GCGCGCAAACCCGTCACGAT

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 713; Mature: 712

Protein sequence:

>713_residues
MSLFNKIVKEFQWGQHKVRLETGEIARQASGAVLVDVEDTVVLATVVGAKSAKPGQDFFPLTVDYIEKTYSAGKIPGGFF
RREGRPSEHETLTSRLIDRPLRPLFPEGFYNEVQVVIHVLSVNPEVPADIPALIGASAALAVSGLPFNGPVGAARVAYVN
NEYVLNPTREQIKASRLDLVVAGTERAVLMVESEADQLPEDVMLGAVVFGHEQMQTAIDAIHELVREGGKPEWDWQPAPK
DEALNARVTELAQPELLAAYQIRDKQARSTKLKEVYAATSAKLEEEALAAGTVAADKATVGNILFDLEAKIVRGQILSGE
PRIDGRDTRTVRPIEIRTGVLPRTHGSALFTRGETQALVVATLGTKGDEQIIDALEGEYRDRFMLHYNMPPFATGETGRV
GSPKRREIGHGRLAKRALVACLPSADEFGYSIRVVSEITESNGSSSMASVCGGCLALMDAGVPMKAHVAGIAMGLILEGN
KFAVLTDILGDEDHLGDMDFKVAGTAEGVTALQMDIKIQGITKEIMQVALAQAKEGRMHILGKMKEAVAGANTQLSEFAP
RMITIKINPEKIRDVIGKGGSVIRALTEETGTTIDISDDGVVTIASTNSEGMAEAKKRIENITAEIEVGQVYEGTVLKLL
DFGAIVNLLPGKDGLLHISEIVNERVKDINDYLKEGQQVKVKVIQTDEKGRVRLSAKALLNEAAGQTDTPPQQ

Sequences:

>Translated_713_residues
MSLFNKIVKEFQWGQHKVRLETGEIARQASGAVLVDVEDTVVLATVVGAKSAKPGQDFFPLTVDYIEKTYSAGKIPGGFF
RREGRPSEHETLTSRLIDRPLRPLFPEGFYNEVQVVIHVLSVNPEVPADIPALIGASAALAVSGLPFNGPVGAARVAYVN
NEYVLNPTREQIKASRLDLVVAGTERAVLMVESEADQLPEDVMLGAVVFGHEQMQTAIDAIHELVREGGKPEWDWQPAPK
DEALNARVTELAQPELLAAYQIRDKQARSTKLKEVYAATSAKLEEEALAAGTVAADKATVGNILFDLEAKIVRGQILSGE
PRIDGRDTRTVRPIEIRTGVLPRTHGSALFTRGETQALVVATLGTKGDEQIIDALEGEYRDRFMLHYNMPPFATGETGRV
GSPKRREIGHGRLAKRALVACLPSADEFGYSIRVVSEITESNGSSSMASVCGGCLALMDAGVPMKAHVAGIAMGLILEGN
KFAVLTDILGDEDHLGDMDFKVAGTAEGVTALQMDIKIQGITKEIMQVALAQAKEGRMHILGKMKEAVAGANTQLSEFAP
RMITIKINPEKIRDVIGKGGSVIRALTEETGTTIDISDDGVVTIASTNSEGMAEAKKRIENITAEIEVGQVYEGTVLKLL
DFGAIVNLLPGKDGLLHISEIVNERVKDINDYLKEGQQVKVKVIQTDEKGRVRLSAKALLNEAAGQTDTPPQQ
>Mature_712_residues
SLFNKIVKEFQWGQHKVRLETGEIARQASGAVLVDVEDTVVLATVVGAKSAKPGQDFFPLTVDYIEKTYSAGKIPGGFFR
REGRPSEHETLTSRLIDRPLRPLFPEGFYNEVQVVIHVLSVNPEVPADIPALIGASAALAVSGLPFNGPVGAARVAYVNN
EYVLNPTREQIKASRLDLVVAGTERAVLMVESEADQLPEDVMLGAVVFGHEQMQTAIDAIHELVREGGKPEWDWQPAPKD
EALNARVTELAQPELLAAYQIRDKQARSTKLKEVYAATSAKLEEEALAAGTVAADKATVGNILFDLEAKIVRGQILSGEP
RIDGRDTRTVRPIEIRTGVLPRTHGSALFTRGETQALVVATLGTKGDEQIIDALEGEYRDRFMLHYNMPPFATGETGRVG
SPKRREIGHGRLAKRALVACLPSADEFGYSIRVVSEITESNGSSSMASVCGGCLALMDAGVPMKAHVAGIAMGLILEGNK
FAVLTDILGDEDHLGDMDFKVAGTAEGVTALQMDIKIQGITKEIMQVALAQAKEGRMHILGKMKEAVAGANTQLSEFAPR
MITIKINPEKIRDVIGKGGSVIRALTEETGTTIDISDDGVVTIASTNSEGMAEAKKRIENITAEIEVGQVYEGTVLKLLD
FGAIVNLLPGKDGLLHISEIVNERVKDINDYLKEGQQVKVKVIQTDEKGRVRLSAKALLNEAAGQTDTPPQQ

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=718, Percent_Identity=37.7437325905292, Blast_Score=439, Evalue=1e-123,
Organism=Escherichia coli, GI145693187, Length=697, Percent_Identity=65.4232424677188, Blast_Score=937, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=724, Percent_Identity=33.1491712707182, Blast_Score=325, Evalue=6e-89,
Organism=Drosophila melanogaster, GI281362905, Length=725, Percent_Identity=35.7241379310345, Blast_Score=413, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24651641, Length=725, Percent_Identity=35.7241379310345, Blast_Score=413, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24651643, Length=725, Percent_Identity=35.7241379310345, Blast_Score=413, Evalue=1e-115,
Organism=Drosophila melanogaster, GI161079377, Length=676, Percent_Identity=35.6508875739645, Blast_Score=380, Evalue=1e-105,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 76971; Mature: 76840

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLFNKIVKEFQWGQHKVRLETGEIARQASGAVLVDVEDTVVLATVVGAKSAKPGQDFFP
CCHHHHHHHHHHCCCEEEEEEHHHHHHHCCCEEEEEECCCEEEEEECCCCCCCCCCCCCC
LTVDYIEKTYSAGKIPGGFFRREGRPSEHETLTSRLIDRPLRPLFPEGFYNEVQVVIHVL
CCHHHHHHHHCCCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHEEEE
SVNPEVPADIPALIGASAALAVSGLPFNGPVGAARVAYVNNEYVLNPTREQIKASRLDLV
ECCCCCCCCCHHHHCCCHHHEEECCCCCCCCCCEEEEEECCCEEECCCHHHHHHHHEEEE
VAGTERAVLMVESEADQLPEDVMLGAVVFGHEQMQTAIDAIHELVREGGKPEWDWQPAPK
EECCCEEEEEEECCHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
DEALNARVTELAQPELLAAYQIRDKQARSTKLKEVYAATSAKLEEEALAAGTVAADKATV
CCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
GNILFDLEAKIVRGQILSGEPRIDGRDTRTVRPIEIRTGVLPRTHGSALFTRGETQALVV
HHEEEEEHHHEEECEEECCCCCCCCCCCCEEEEEEEECCCCCCCCCCEEEECCCCCEEEE
ATLGTKGDEQIIDALEGEYRDRFMLHYNMPPFATGETGRVGSPKRREIGHGRLAKRALVA
EEECCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHH
CLPSADEFGYSIRVVSEITESNGSSSMASVCGGCLALMDAGVPMKAHVAGIAMGLILEGN
HCCCCHHCCEEEEEEEHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHEEEEEEEECC
KFAVLTDILGDEDHLGDMDFKVAGTAEGVTALQMDIKIQGITKEIMQVALAQAKEGRMHI
CEEEEEECCCCCCCCCCCCEEEECCCCCCEEEEEEEEEEHHHHHHHHHHHHHHCCCCHHH
LGKMKEAVAGANTQLSEFAPRMITIKINPEKIRDVIGKGGSVIRALTEETGTTIDISDDG
HHHHHHHHCCCCCCHHHCCCEEEEEEECHHHHHHHHCCCCHHHHHHHHCCCCEEEECCCC
VVTIASTNSEGMAEAKKRIENITAEIEVGQVYEGTVLKLLDFGAIVNLLPGKDGLLHISE
EEEEEECCCCHHHHHHHHHHHCEEEEEECCEECCHHHHHHHHHHHHHHCCCCCCCCHHHH
IVNERVKDINDYLKEGQQVKVKVIQTDEKGRVRLSAKALLNEAAGQTDTPPQQ
HHHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SLFNKIVKEFQWGQHKVRLETGEIARQASGAVLVDVEDTVVLATVVGAKSAKPGQDFFP
CHHHHHHHHHHCCCEEEEEEHHHHHHHCCCEEEEEECCCEEEEEECCCCCCCCCCCCCC
LTVDYIEKTYSAGKIPGGFFRREGRPSEHETLTSRLIDRPLRPLFPEGFYNEVQVVIHVL
CCHHHHHHHHCCCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHEEEE
SVNPEVPADIPALIGASAALAVSGLPFNGPVGAARVAYVNNEYVLNPTREQIKASRLDLV
ECCCCCCCCCHHHHCCCHHHEEECCCCCCCCCCEEEEEECCCEEECCCHHHHHHHHEEEE
VAGTERAVLMVESEADQLPEDVMLGAVVFGHEQMQTAIDAIHELVREGGKPEWDWQPAPK
EECCCEEEEEEECCHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
DEALNARVTELAQPELLAAYQIRDKQARSTKLKEVYAATSAKLEEEALAAGTVAADKATV
CCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
GNILFDLEAKIVRGQILSGEPRIDGRDTRTVRPIEIRTGVLPRTHGSALFTRGETQALVV
HHEEEEEHHHEEECEEECCCCCCCCCCCCEEEEEEEECCCCCCCCCCEEEECCCCCEEEE
ATLGTKGDEQIIDALEGEYRDRFMLHYNMPPFATGETGRVGSPKRREIGHGRLAKRALVA
EEECCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHH
CLPSADEFGYSIRVVSEITESNGSSSMASVCGGCLALMDAGVPMKAHVAGIAMGLILEGN
HCCCCHHCCEEEEEEEHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHEEEEEEEECC
KFAVLTDILGDEDHLGDMDFKVAGTAEGVTALQMDIKIQGITKEIMQVALAQAKEGRMHI
CEEEEEECCCCCCCCCCCCEEEECCCCCCEEEEEEEEEEHHHHHHHHHHHHHHCCCCHHH
LGKMKEAVAGANTQLSEFAPRMITIKINPEKIRDVIGKGGSVIRALTEETGTTIDISDDG
HHHHHHHHCCCCCCHHHCCCEEEEEEECHHHHHHHHCCCCHHHHHHHHCCCCEEEECCCC
VVTIASTNSEGMAEAKKRIENITAEIEVGQVYEGTVLKLLDFGAIVNLLPGKDGLLHISE
EEEEEECCCCHHHHHHHHHHHCEEEEEECCEECCHHHHHHHHHHHHHHCCCCCCCCHHHH
IVNERVKDINDYLKEGQQVKVKVIQTDEKGRVRLSAKALLNEAAGQTDTPPQQ
HHHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA