BacMap

Definition	Burkholderia thailandensis E264 chromosome chromosome I, complete sequence.
Accession	NC_007651
Length	3,809,201

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The map label for this gene is linX [H]

Identifier: 83718723

GI number: 83718723

Start: 1328687

End: 1329502

Strand: Direct

Name: linX [H]

Synonym: BTH_I1182

Alternate gene names: 83718723

Gene position: 1328687-1329502 (Clockwise)

Preceding gene: 83720442

Following gene: 83721038

Centisome position: 34.88

GC content: 70.71

Gene sequence:

>816_bases
ATGAGCGACATTGCCTCGCATCGCGAATCGAACGGCGCGCGCAGCGCGCAAGACGAGCGTTACGCACGCTACCCGAGCCT
CGCCGGGCGCGCGGTGCTGATCACGGGCGGCGCGACGGGCATCGGCGCGTCGTTCGTCGAACACTTCGCGCGGCAGGGCG
CGCGCGTCGCGTTCGTCGATCTCGACGAACAAGCCGCGCGCGCGCTCGCCGCGCGGCTTGCCGACGCCGCGCACGAACCG
GTGTTCGTCGCGTGCGACCTGACCGACATCGCCGCGCTGCGCGGCGCGATCGAAGCGATCCGCGCGCGCATCGGCCCGAT
CGCGGCGCTCGTGAACAACGCGGCGAACGACGTGCGCCACGCGATCGCCGACGTGACGCCCGATTCGTTCGACGCGTGCA
TCGCGGTGAACCTGCGCCATCAGTTCTTCGCCGCGCAGGCGGTGATCGACGACATGAAGCGGCTGGGCGGCGGCTCGATC
GTCAATCTCGGCTCGATCAGCTGGATGCTGAAGAACGCCGGCTATCCGGTCTACGCGAGCGCGAAGGCGGCGGTGCAGGG
GCTCACGCGCGCGCTCGCGCGCGAGCTCGGGCCGTTCGGCATTCGCGTGAATACGCTCGTGCCCGGCTGGGTGATGACCG
ACAAGCAGCGTCGCTTGTGGCTCGACGACGCGGGCCGCGCGGCGATCAAGGCGGGGCAGTGCATCGACGCGGAGCTGCTT
CCGGGCGACCTCGCGCGGATGGCGCTCTTTCTCGCGGCGGACGACAGCCGGATGATCACCGCGCAGGACGTTGTCGTCGA
CGGCGGCTGGGCATAG

Upstream 100 bases:

>100_bases
TGGTGCGCGGCGCGATCCTGCTCGCCGCCGTGCTGCTCGACCAGTTGAAGAATCGCGGCGCGCGCGATTGAGTGCATCGT
CCATTTCCGGGAACGAACAG

Downstream 100 bases:

>100_bases
CATTCGTTCGGATGACGAAGCGTGACGAAAGCAGGAGGACCGAATCATGACGGCGACGCAATCTCGACCCACGGCCACCG
ACGCGGCGCAAACCTTGCAG

Product: short chain dehydrogenase/reductase family oxidoreductase

Products: (3R)-hydroxyacyl-[acyl-carrier-protein]; NADP; NADPH; Proton; beta-ketoacyl-ACP [C]

Alternate protein names: 2,5-DDOL dehydrogenase [H]

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MSDIASHRESNGARSAQDERYARYPSLAGRAVLITGGATGIGASFVEHFARQGARVAFVDLDEQAARALAARLADAAHEP
VFVACDLTDIAALRGAIEAIRARIGPIAALVNNAANDVRHAIADVTPDSFDACIAVNLRHQFFAAQAVIDDMKRLGGGSI
VNLGSISWMLKNAGYPVYASAKAAVQGLTRALARELGPFGIRVNTLVPGWVMTDKQRRLWLDDAGRAAIKAGQCIDAELL
PGDLARMALFLAADDSRMITAQDVVVDGGWA

Sequences:

>Translated_271_residues
MSDIASHRESNGARSAQDERYARYPSLAGRAVLITGGATGIGASFVEHFARQGARVAFVDLDEQAARALAARLADAAHEP
VFVACDLTDIAALRGAIEAIRARIGPIAALVNNAANDVRHAIADVTPDSFDACIAVNLRHQFFAAQAVIDDMKRLGGGSI
VNLGSISWMLKNAGYPVYASAKAAVQGLTRALARELGPFGIRVNTLVPGWVMTDKQRRLWLDDAGRAAIKAGQCIDAELL
PGDLARMALFLAADDSRMITAQDVVVDGGWA
>Mature_270_residues
SDIASHRESNGARSAQDERYARYPSLAGRAVLITGGATGIGASFVEHFARQGARVAFVDLDEQAARALAARLADAAHEPV
FVACDLTDIAALRGAIEAIRARIGPIAALVNNAANDVRHAIADVTPDSFDACIAVNLRHQFFAAQAVIDDMKRLGGGSIV
NLGSISWMLKNAGYPVYASAKAAVQGLTRALARELGPFGIRVNTLVPGWVMTDKQRRLWLDDAGRAAIKAGQCIDAELLP
GDLARMALFLAADDSRMITAQDVVVDGGWA

Specific function: Catalyzes the degradation of 2,5-dichloro-2,5- cyclohexadiene-1,4-diol (2,5-DDOL) into 2,5-dichlorohydroquinone (2,5-DCHQ). LinX appears not to be involved in gamma-HCH degradation pathway [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI32483357, Length=261, Percent_Identity=32.9501915708812, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI15277342, Length=253, Percent_Identity=33.9920948616601, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI7705925, Length=251, Percent_Identity=35.0597609561753, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI40254992, Length=237, Percent_Identity=29.535864978903, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI31542939, Length=193, Percent_Identity=32.1243523316062, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI19923817, Length=254, Percent_Identity=27.5590551181102, Blast_Score=88, Evalue=8e-18,
Organism=Homo sapiens, GI126723750, Length=266, Percent_Identity=25.9398496240602, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI59889578, Length=233, Percent_Identity=27.8969957081545, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI4503817, Length=204, Percent_Identity=25.9803921568627, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI66933014, Length=255, Percent_Identity=27.843137254902, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI224922801, Length=171, Percent_Identity=30.4093567251462, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI4758504, Length=200, Percent_Identity=29.5, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI126723191, Length=191, Percent_Identity=29.8429319371728, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI10190704, Length=245, Percent_Identity=29.3877551020408, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI210032110, Length=211, Percent_Identity=27.0142180094787, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI20149619, Length=189, Percent_Identity=29.1005291005291, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI5031737, Length=243, Percent_Identity=24.6913580246914, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI302344755, Length=103, Percent_Identity=37.8640776699029, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI1787335, Length=247, Percent_Identity=32.3886639676113, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI87082100, Length=251, Percent_Identity=32.6693227091633, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1790717, Length=248, Percent_Identity=31.4516129032258, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1786812, Length=252, Percent_Identity=31.3492063492063, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1789208, Length=251, Percent_Identity=27.8884462151394, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI87082160, Length=257, Percent_Identity=28.4046692607004, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI2367365, Length=246, Percent_Identity=29.6747967479675, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1789378, Length=253, Percent_Identity=28.0632411067194, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787905, Length=256, Percent_Identity=28.90625, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI2367175, Length=188, Percent_Identity=29.7872340425532, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788459, Length=251, Percent_Identity=27.4900398406374, Blast_Score=72, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI25147288, Length=253, Percent_Identity=32.0158102766798, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI115534694, Length=243, Percent_Identity=31.2757201646091, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17555706, Length=247, Percent_Identity=29.5546558704453, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71994600, Length=245, Percent_Identity=28.1632653061224, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI72000259, Length=269, Percent_Identity=29.368029739777, Blast_Score=87, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17560150, Length=261, Percent_Identity=29.1187739463602, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17563726, Length=266, Percent_Identity=29.3233082706767, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17559104, Length=268, Percent_Identity=28.7313432835821, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17561402, Length=250, Percent_Identity=28, Blast_Score=80, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17538480, Length=266, Percent_Identity=27.8195488721804, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71994604, Length=244, Percent_Identity=26.2295081967213, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17567345, Length=190, Percent_Identity=27.3684210526316, Blast_Score=78, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17562904, Length=269, Percent_Identity=29.368029739777, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17560332, Length=265, Percent_Identity=27.9245283018868, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17562990, Length=271, Percent_Identity=29.1512915129151, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17562908, Length=259, Percent_Identity=27.4131274131274, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17560220, Length=271, Percent_Identity=27.6752767527675, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17531453, Length=261, Percent_Identity=27.9693486590038, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17551412, Length=270, Percent_Identity=29.2592592592593, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17562910, Length=271, Percent_Identity=26.9372693726937, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17544670, Length=262, Percent_Identity=26.7175572519084, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI193203117, Length=189, Percent_Identity=25.3968253968254, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17562906, Length=259, Percent_Identity=26.6409266409266, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17538182, Length=202, Percent_Identity=28.7128712871287, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI193203115, Length=187, Percent_Identity=25.668449197861, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17508651, Length=251, Percent_Identity=25.4980079681275, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17560676, Length=251, Percent_Identity=25.0996015936255, Blast_Score=64, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17508895, Length=198, Percent_Identity=25.2525252525253, Blast_Score=64, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6324126, Length=254, Percent_Identity=27.1653543307087, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21357041, Length=254, Percent_Identity=31.496062992126, Blast_Score=104, Evalue=7e-23,
Organism=Drosophila melanogaster, GI28571526, Length=249, Percent_Identity=29.718875502008, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24643142, Length=248, Percent_Identity=28.2258064516129, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24639444, Length=248, Percent_Identity=29.0322580645161, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI23397609, Length=249, Percent_Identity=29.718875502008, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24644339, Length=249, Percent_Identity=28.1124497991968, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24644337, Length=248, Percent_Identity=30.241935483871, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21355319, Length=255, Percent_Identity=26.2745098039216, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI281364892, Length=187, Percent_Identity=29.9465240641711, Blast_Score=78, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24584752, Length=236, Percent_Identity=29.2372881355932, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI17737361, Length=201, Percent_Identity=30.3482587064677, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24640492, Length=168, Percent_Identity=27.9761904761905, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21358495, Length=192, Percent_Identity=26.0416666666667, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21356301, Length=196, Percent_Identity=27.0408163265306, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24581726, Length=204, Percent_Identity=25.4901960784314, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.100 [C]

Molecular weight: Translated: 28677; Mature: 28546

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDIASHRESNGARSAQDERYARYPSLAGRAVLITGGATGIGASFVEHFARQGARVAFVD
CCCHHHHHHCCCCCCCHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEEE
LDEQAARALAARLADAAHEPVFVACDLTDIAALRGAIEAIRARIGPIAALVNNAANDVRH
CCHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIADVTPDSFDACIAVNLRHQFFAAQAVIDDMKRLGGGSIVNLGSISWMLKNAGYPVYAS
HHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHCCCCCEEEC
AKAAVQGLTRALARELGPFGIRVNTLVPGWVMTDKQRRLWLDDAGRAAIKAGQCIDAELL
HHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEECCCCEEEECCCCCHHHHCCCCCCCCCC
PGDLARMALFLAADDSRMITAQDVVVDGGWA
CHHHHHHEEHEECCCCCEEEEEEEEECCCCC
>Mature Secondary Structure 
SDIASHRESNGARSAQDERYARYPSLAGRAVLITGGATGIGASFVEHFARQGARVAFVD
CCHHHHHHCCCCCCCHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEEE
LDEQAARALAARLADAAHEPVFVACDLTDIAALRGAIEAIRARIGPIAALVNNAANDVRH
CCHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIADVTPDSFDACIAVNLRHQFFAAQAVIDDMKRLGGGSIVNLGSISWMLKNAGYPVYAS
HHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHCCCCCEEEC
AKAAVQGLTRALARELGPFGIRVNTLVPGWVMTDKQRRLWLDDAGRAAIKAGQCIDAELL
HHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEECCCCEEEECCCCCHHHHCCCCCCCCCC
PGDLARMALFLAADDSRMITAQDVVVDGGWA
CHHHHHHEEHEECCCCCEEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Oxoacyl-[acyl-carrier-protein]; NADPH; D-3-hydroxy-acyl-ACP; NADP [C]

Specific reaction: Oxoacyl-[acyl-carrier-protein] + NADPH = (3R)-hydroxyacyl-[acyl-carrier-protein] + NADP+ D-3-hydroxy-acyl-ACP + NADP = NADPH + Proton + beta-ketoacyl-ACP [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7515041; 12450824 [H]