Click here to switch to the map view.

The map label for this gene is dctA2 [H]

Identifier: 83718519

GI number: 83718519

Start: 1348887

End: 1350209

Strand: Direct

Name: dctA2 [H]

Synonym: BTH_I1203

Alternate gene names: 83718519

Gene position: 1348887-1350209 (Clockwise)

Preceding gene: 161723134

Following gene: 83720932

Centisome position: 35.41

GC content: 65.53

Gene sequence:

>1323_bases
GTGTCGAAGTTTCTCAATTCGCTGTTTGGTCGGGTGGTCATCGCGCTGTTGATCGGCGTGGCGCTCGGCGCTTTCTTCCC
GCACTTCGCGCAGTCGCTGCGACCGTTGGGTGACGGGTTCCTGAAGTTGATCAAGATGGTGATCGGGCCGATTGTGTTCT
GCGTCGTCGTAAGCGGCATGGCGAACGCGGGCGATCTGAAGAAGGTCGGCCGCGTCGGCCTGAAGGCCGTCGTCTACTTC
GAGGCGATGACGACGCTCGCGCTCGGCATCGGCCTCCTGCTCGCATACCTGACGAAGCCGGGCGTCGGGATGAACGTCGA
TCTGCATACGCTCGACCCCGCATCGCTCGCCGAATACGCGAAGAACGCGCAGAGCCTGAAGGATACGGCGGGCTACCTGC
TGAAGATCATCCCCGAGACCGCGTTCGACGCGTTCGCGAAGGGCGACATCCTGCAGATCCTCGTGTTCTCGGTGCTGTTC
GGCTCCGCGCTGTCGCTGCTCGGCGACAAGGCGAAGCGCGTGAACACGCTGATCGACGAGTTCGCGCAGGTGTTCTTCCG
CGTGATGGGCTTCATCATCAAGCTCGCGCCGCTTGGCGTGCTCGGCGCGATCGCGTTCACGACCGGCAAGTACGGCGTCG
CGTCGCTCAAGCAACTGGGCCTGCTCGTGATCGTGTTCTACGCGAGCTGCGTCGTGTTCGTCGCGGTCGTGCTCGGCGTG
GTGATGAAGCTCGCGGGCTTCTCGGTGTTCAAGCTGATCCGCTACCTGCGCGAAGAGCTCCTGATCGTGCTCGGCACCGC
GTCGTCGGACGCCGTGCTGCCGCAGGTGATGCGCAAGCTCGAATGGATGGGCGTGAAGGATTCGACGGTCGGCCTCGTGA
TTCCGACGGGCTACTCGTTCAACCTCGACGGCTTCTCGATCTACCTGACGCTCGCCGTGCTCTTCATCGCGCAGGCGACC
AACACGCCGCTGTCGGTGCACGATCTGATCGTCGTGCTGCTCGTGTCGCTCGTCACGTCGAAGGGCGCGCACGGCATCCC
GGGCTCGGCGATCGTGATTCTCGCCGCGACGCTGTCGGCGATCCCCGCGCTGCCCGTGCTCGGCCTCGTGCTGATCCTGC
CCGTCGACTGGTTCGTCGGCATCGCGCGCGCGATCACGAACCTGCTCGGCAACTGCGTCGCGACGATCGTCGTCGCGGTG
TGGGAGCGCGACATCGATCGCGCCCGCGCGCAGCGCGTGCTCGACGAGGAACTGCGCTACGTGCCGGCTGGCGAGGAGCG
CTCGAACGGCGCGATCGCGGACGGCGGCGCGCACGCGCTCTGA

Upstream 100 bases:

>100_bases
CCTTGACTCGACTATATCGGCTTCCTACTATCGTCCATAACAGTACTGGTCGGACCAGTATGAACAGACGAATCCGCAAC
CGTTGTGATGGGAGAACGCC

Downstream 100 bases:

>100_bases
CGCGGCGCCGCCCGGCATTATCGCGCGCCGGCGCGGCAGGTCGCGCCGGCGCTTCGTTTTTCAGGTTTTCGGTTTTTCAG
CTTTTCGGACACACATCCAA

Product: C4-dicarboxylate transporter DctA

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MSKFLNSLFGRVVIALLIGVALGAFFPHFAQSLRPLGDGFLKLIKMVIGPIVFCVVVSGMANAGDLKKVGRVGLKAVVYF
EAMTTLALGIGLLLAYLTKPGVGMNVDLHTLDPASLAEYAKNAQSLKDTAGYLLKIIPETAFDAFAKGDILQILVFSVLF
GSALSLLGDKAKRVNTLIDEFAQVFFRVMGFIIKLAPLGVLGAIAFTTGKYGVASLKQLGLLVIVFYASCVVFVAVVLGV
VMKLAGFSVFKLIRYLREELLIVLGTASSDAVLPQVMRKLEWMGVKDSTVGLVIPTGYSFNLDGFSIYLTLAVLFIAQAT
NTPLSVHDLIVVLLVSLVTSKGAHGIPGSAIVILAATLSAIPALPVLGLVLILPVDWFVGIARAITNLLGNCVATIVVAV
WERDIDRARAQRVLDEELRYVPAGEERSNGAIADGGAHAL

Sequences:

>Translated_440_residues
MSKFLNSLFGRVVIALLIGVALGAFFPHFAQSLRPLGDGFLKLIKMVIGPIVFCVVVSGMANAGDLKKVGRVGLKAVVYF
EAMTTLALGIGLLLAYLTKPGVGMNVDLHTLDPASLAEYAKNAQSLKDTAGYLLKIIPETAFDAFAKGDILQILVFSVLF
GSALSLLGDKAKRVNTLIDEFAQVFFRVMGFIIKLAPLGVLGAIAFTTGKYGVASLKQLGLLVIVFYASCVVFVAVVLGV
VMKLAGFSVFKLIRYLREELLIVLGTASSDAVLPQVMRKLEWMGVKDSTVGLVIPTGYSFNLDGFSIYLTLAVLFIAQAT
NTPLSVHDLIVVLLVSLVTSKGAHGIPGSAIVILAATLSAIPALPVLGLVLILPVDWFVGIARAITNLLGNCVATIVVAV
WERDIDRARAQRVLDEELRYVPAGEERSNGAIADGGAHAL
>Mature_439_residues
SKFLNSLFGRVVIALLIGVALGAFFPHFAQSLRPLGDGFLKLIKMVIGPIVFCVVVSGMANAGDLKKVGRVGLKAVVYFE
AMTTLALGIGLLLAYLTKPGVGMNVDLHTLDPASLAEYAKNAQSLKDTAGYLLKIIPETAFDAFAKGDILQILVFSVLFG
SALSLLGDKAKRVNTLIDEFAQVFFRVMGFIIKLAPLGVLGAIAFTTGKYGVASLKQLGLLVIVFYASCVVFVAVVLGVV
MKLAGFSVFKLIRYLREELLIVLGTASSDAVLPQVMRKLEWMGVKDSTVGLVIPTGYSFNLDGFSIYLTLAVLFIAQATN
TPLSVHDLIVVLLVSLVTSKGAHGIPGSAIVILAATLSAIPALPVLGLVLILPVDWFVGIARAITNLLGNCVATIVVAVW
ERDIDRARAQRVLDEELRYVPAGEERSNGAIADGGAHAL

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI169790839, Length=468, Percent_Identity=26.2820512820513, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI4827012, Length=274, Percent_Identity=30.6569343065693, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI40254478, Length=441, Percent_Identity=24.7165532879819, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI21314632, Length=440, Percent_Identity=25.4545454545455, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI5032093, Length=402, Percent_Identity=26.1194029850746, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI66773030, Length=408, Percent_Identity=25.2450980392157, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI194239697, Length=505, Percent_Identity=23.7623762376238, Blast_Score=99, Evalue=9e-21,
Organism=Homo sapiens, GI223468566, Length=277, Percent_Identity=27.7978339350181, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI223468564, Length=277, Percent_Identity=27.7978339350181, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI262359914, Length=391, Percent_Identity=25.3196930946292, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI301601644, Length=204, Percent_Identity=29.4117647058824, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI1789947, Length=427, Percent_Identity=50.5854800936768, Blast_Score=432, Evalue=1e-122,
Organism=Escherichia coli, GI1790514, Length=417, Percent_Identity=37.8896882494005, Blast_Score=280, Evalue=1e-76,
Organism=Escherichia coli, GI1788024, Length=406, Percent_Identity=23.6453201970443, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789473, Length=218, Percent_Identity=27.0642201834862, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI193206654, Length=451, Percent_Identity=25.2771618625277, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17537407, Length=384, Percent_Identity=26.0416666666667, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI71996953, Length=446, Percent_Identity=26.0089686098655, Blast_Score=112, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI193206505, Length=480, Percent_Identity=25.8333333333333, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17541374, Length=406, Percent_Identity=25.3694581280788, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71983099, Length=450, Percent_Identity=24.2222222222222, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71983106, Length=450, Percent_Identity=24.2222222222222, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24583025, Length=436, Percent_Identity=23.8532110091743, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI17137668, Length=436, Percent_Identity=23.8532110091743, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI24583023, Length=436, Percent_Identity=23.8532110091743, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI281360481, Length=463, Percent_Identity=28.0777537796976, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI281360483, Length=463, Percent_Identity=28.0777537796976, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI17137666, Length=463, Percent_Identity=28.0777537796976, Blast_Score=102, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 46715; Mature: 46584

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1 ; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SKFLNSLFGRVVIALLIGVALGAFFPHFAQSLRPLGDGFLKLIKMVIGPIVFCVVVSGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC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CCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]