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The map label for this gene is 83718404

Identifier: 83718404

GI number: 83718404

Start: 1601258

End: 1603354

Strand: Direct

Name: 83718404

Synonym: BTH_I1420

Alternate gene names: NA

Gene position: 1601258-1603354 (Clockwise)

Preceding gene: 83720879

Following gene: 83719333

Centisome position: 42.04

GC content: 70.43

Gene sequence:

>2097_bases
ATGACGACGATCAGGGGCGGATCGAGGCGCGCGTCGCTGCCGGCGCTCGCGCTTCTCGGCGTGCTGCTCGGCGCATGCCA
CAGCGACGACAACGCGCAGGTGAACACGCTGCCCGGCTTTGTCTCGGGCAACGTGCGCAAGACGGCCTACGACGGCGCGA
GCGATGATCTGCTGACGGCGGGGCTCGGCAAGACAGGCCTCGGCAGCGACACGCGGCCCGGCTTCGCGAACCCGGCGCAA
CCGACCGCGGCGGAACTGCGGCGTCTTGCGATCTACTCGAACTACCGCGCGCTCGTCGACATCACGCCGAACGGCGGCTA
CGGGCGCTTCTGGGGGCCGAACGTCGACCTCGCGGGCAACGATACGCTCGGCGAAGGCAAGATTGCCGGCACCGAATATC
TCGCGTACTCCGACGACGGCAGCGGACGCAAGAACGTCACGCTGCTCGTGCAGGTGCCGGCGAGCTTCGATCCGGCGAAT
CCGTGCATCGTGACGGCGACGTCGTCGGGCTCGCGCGGCGTCTACGGCGCGATCGCCGCGGCGGGCGAGTGGGGGCTGAA
GCGCGGCTGCGCGGTCGCGTACAACGACAAGGGCGGCGGCAACGGCGCGCACGAGATCGGCACGGGCGTCGTCACGCTGA
TCGACGGCACGCTCGCGAGCGCATCGAGCGCGGGCAGCGCGAGCCTCTTCACCGCGAGCGAATCGAGCAGCGCGCTCGCC
GCGTTCAACAGCGCGTTTCCGAACCGCTACGCGTACAAACATGCGCATTCGCAGCAGAATCCCGAGCAGGACTGGGGGCG
CGTGACGCTGCAGGCGGTCGAGTTCGCATACTGGGCGCTGAACGAGCAGTTCGGTCCCGCCATCGACGGCGCGCGGCACG
GCGTCCGCTATCGGCCGGGCGACATCACGACGATCGCCGCATCGGTCAGCAACGGCGGCGCAGCGGCGCTCGCGGCAGCC
GAGCAGGACACGCGAGGCTGGATCACCGCGGTCGTCGTCGGCGAGCCGCAGATCAACGTGCGCATGACGCCGGGCGTGAC
CGTCGAGCAGGGCGGCGTGCCTGCGCCGTCGTTCGGCCGGCCGCTCGCCGATTACGCGACGCTCGCGAACCTGCTGCAAC
CATGCGCGGCGGCGGCCGTCGCCGCGGTGGGCGCGCCTTATCTCAGCGCGCTGCCCGTCGGGCTCACGCAGTCGATTCGC
GTGCAGCGCTGCGCGACGCTCGCGGCGGCCGGGCTCGTGTCGGGCGCCGATACGGCGAGCCAGGCGAACGACGCGCTCGC
GCAACTGCACGCGGCCGGCTACCTCGCCGATTCCGACCTGCTGCAGGCGCCGATGTGGGATTCGCAGGCGATTCCGGCGA
TCGCGGTTACGTATGCGAACGCTTACACGCGCTCGCGCGTCACCGATAACCTCTGCAACTTCAGCTTCGCGACGACGAAC
TCCGTGACGGGCGCCGTTGCTCCGCCCGCCACGTCGCCGATGACGAGCCTGTTCGGCGCGGGCAACGGCGTGCCGCCGAC
GAACGGGATCAATCTCGTGTTCAACGGCGCGTCGGGCGGCGTCGACCACCGGCTTGCGACGCCGGACGCGAGCTTTGCGG
GCGCGTTCTGCCTGCGCCAGCTGTGGACCGCGAACCAGCTCGGCATCGGCGCGAATGTCGACGCGGTGCGCGTGGCCGCG
AATCTGCAGCGCAAGCCGGCGATCATCGTGCAAGGCCGCAGCGACGCGCTCGTGCCCGTCAATCACGCGTCGCGCGCGTA
TGTCGCGCAAAACAGCGCGACCGAGGGCCGCGCGAGCCAGCTGTCGTTCTACGAAGTGACGAACGGCCAGCACTTCGACG
CGTTCCTGTCGGTGCCCGGCTTCGACACGCGCTTCGTGCCGGTTCACTACTACGACGAACAGGCGCTCAACCTGATGTGG
AATCACCTGAAGAGCGGCGCGCCGCTGCCGCCGTCGCAGGTGATCCGCACGGTGCCGCGCGGCGGCGTGCCGGGCGCCGC
GCCCGCGCTGTCGACGGCGAATCTGCCGCCGATCGTCCAGTCGCCGGGCTCGAACGCGATCACCGTGAACGCGGGGGTGA
TCGACGTGCCGCTCTGA

Upstream 100 bases:

>100_bases
CAACGACGCCGCCCGCCGCGCGCGCCGGCCGTGCCTGGGCGCGGCGCGCGCCGCCCGGCGAGCGGGCGATTCGAACCAAT
CAGGCAGACTGGGGGCAACA

Downstream 100 bases:

>100_bases
CGCGCCGCGCGCGCTCGCTGGTCACGACGGCCGGCCCGCACATACGTGCGGCCGGCCGTTTTTCATGGGCCGGCGAGCCT
TCGCCGCAAAGGCGCGATTC

Product: D-(-)-3-hydroxybutyrate-oligomer hydrolase

Products: NA

Alternate protein names: 3HB-oligomer hydrolase; 3HBOH [H]

Number of amino acids: Translated: 698; Mature: 697

Protein sequence:

>698_residues
MTTIRGGSRRASLPALALLGVLLGACHSDDNAQVNTLPGFVSGNVRKTAYDGASDDLLTAGLGKTGLGSDTRPGFANPAQ
PTAAELRRLAIYSNYRALVDITPNGGYGRFWGPNVDLAGNDTLGEGKIAGTEYLAYSDDGSGRKNVTLLVQVPASFDPAN
PCIVTATSSGSRGVYGAIAAAGEWGLKRGCAVAYNDKGGGNGAHEIGTGVVTLIDGTLASASSAGSASLFTASESSSALA
AFNSAFPNRYAYKHAHSQQNPEQDWGRVTLQAVEFAYWALNEQFGPAIDGARHGVRYRPGDITTIAASVSNGGAAALAAA
EQDTRGWITAVVVGEPQINVRMTPGVTVEQGGVPAPSFGRPLADYATLANLLQPCAAAAVAAVGAPYLSALPVGLTQSIR
VQRCATLAAAGLVSGADTASQANDALAQLHAAGYLADSDLLQAPMWDSQAIPAIAVTYANAYTRSRVTDNLCNFSFATTN
SVTGAVAPPATSPMTSLFGAGNGVPPTNGINLVFNGASGGVDHRLATPDASFAGAFCLRQLWTANQLGIGANVDAVRVAA
NLQRKPAIIVQGRSDALVPVNHASRAYVAQNSATEGRASQLSFYEVTNGQHFDAFLSVPGFDTRFVPVHYYDEQALNLMW
NHLKSGAPLPPSQVIRTVPRGGVPGAAPALSTANLPPIVQSPGSNAITVNAGVIDVPL

Sequences:

>Translated_698_residues
MTTIRGGSRRASLPALALLGVLLGACHSDDNAQVNTLPGFVSGNVRKTAYDGASDDLLTAGLGKTGLGSDTRPGFANPAQ
PTAAELRRLAIYSNYRALVDITPNGGYGRFWGPNVDLAGNDTLGEGKIAGTEYLAYSDDGSGRKNVTLLVQVPASFDPAN
PCIVTATSSGSRGVYGAIAAAGEWGLKRGCAVAYNDKGGGNGAHEIGTGVVTLIDGTLASASSAGSASLFTASESSSALA
AFNSAFPNRYAYKHAHSQQNPEQDWGRVTLQAVEFAYWALNEQFGPAIDGARHGVRYRPGDITTIAASVSNGGAAALAAA
EQDTRGWITAVVVGEPQINVRMTPGVTVEQGGVPAPSFGRPLADYATLANLLQPCAAAAVAAVGAPYLSALPVGLTQSIR
VQRCATLAAAGLVSGADTASQANDALAQLHAAGYLADSDLLQAPMWDSQAIPAIAVTYANAYTRSRVTDNLCNFSFATTN
SVTGAVAPPATSPMTSLFGAGNGVPPTNGINLVFNGASGGVDHRLATPDASFAGAFCLRQLWTANQLGIGANVDAVRVAA
NLQRKPAIIVQGRSDALVPVNHASRAYVAQNSATEGRASQLSFYEVTNGQHFDAFLSVPGFDTRFVPVHYYDEQALNLMW
NHLKSGAPLPPSQVIRTVPRGGVPGAAPALSTANLPPIVQSPGSNAITVNAGVIDVPL
>Mature_697_residues
TTIRGGSRRASLPALALLGVLLGACHSDDNAQVNTLPGFVSGNVRKTAYDGASDDLLTAGLGKTGLGSDTRPGFANPAQP
TAAELRRLAIYSNYRALVDITPNGGYGRFWGPNVDLAGNDTLGEGKIAGTEYLAYSDDGSGRKNVTLLVQVPASFDPANP
CIVTATSSGSRGVYGAIAAAGEWGLKRGCAVAYNDKGGGNGAHEIGTGVVTLIDGTLASASSAGSASLFTASESSSALAA
FNSAFPNRYAYKHAHSQQNPEQDWGRVTLQAVEFAYWALNEQFGPAIDGARHGVRYRPGDITTIAASVSNGGAAALAAAE
QDTRGWITAVVVGEPQINVRMTPGVTVEQGGVPAPSFGRPLADYATLANLLQPCAAAAVAAVGAPYLSALPVGLTQSIRV
QRCATLAAAGLVSGADTASQANDALAQLHAAGYLADSDLLQAPMWDSQAIPAIAVTYANAYTRSRVTDNLCNFSFATTNS
VTGAVAPPATSPMTSLFGAGNGVPPTNGINLVFNGASGGVDHRLATPDASFAGAFCLRQLWTANQLGIGANVDAVRVAAN
LQRKPAIIVQGRSDALVPVNHASRAYVAQNSATEGRASQLSFYEVTNGQHFDAFLSVPGFDTRFVPVHYYDEQALNLMWN
HLKSGAPLPPSQVIRTVPRGGVPGAAPALSTANLPPIVQSPGSNAITVNAGVIDVPL

Specific function: Participates in the degradation of poly-3- hydroxybutyrate (PHB). It works downstream of poly(3- hydroxybutyrate) depolymerase, hydrolyzing D(-)-3-hydroxybutyrate oligomers of various length (3HB-oligomers) into 3HB-monomers [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-(-)-3-hydroxybutyrate oligomer hydrolase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016582 [H]

Pfam domain/function: PF10605 3HBOH [H]

EC number: =3.1.1.22 [H]

Molecular weight: Translated: 71669; Mature: 71537

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTIRGGSRRASLPALALLGVLLGACHSDDNAQVNTLPGFVSGNVRKTAYDGASDDLLTA
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCEECCCEEEEECCCCCCCEEEE
GLGKTGLGSDTRPGFANPAQPTAAELRRLAIYSNYRALVDITPNGGYGRFWGPNVDLAGN
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHEECCEEEEEECCCCCCCCCCCCCCCCCCC
DTLGEGKIAGTEYLAYSDDGSGRKNVTLLVQVPASFDPANPCIVTATSSGSRGVYGAIAA
CCCCCCCCCCCEEEEECCCCCCCCCEEEEEEECCCCCCCCCEEEEEECCCCCCCEEEEEE
AGEWGLKRGCAVAYNDKGGGNGAHEIGTGVVTLIDGTLASASSAGSASLFTASESSSALA
CCCCCCCCCCEEEECCCCCCCCHHHHCCCEEEEEECCHHCCCCCCCEEEEEECCCCCHHH
AFNSAFPNRYAYKHAHSQQNPEQDWGRVTLQAVEFAYWALNEQFGPAIDGARHGVRYRPG
HHHHCCCCCHHHHHHHCCCCCCHHHHHEEHHHHHHHHHHHCCCCCCCCCHHHCCCEECCC
DITTIAASVSNGGAAALAAAEQDTRGWITAVVVGEPQINVRMTPGVTVEQGGVPAPSFGR
CEEEEEEECCCCCEEEEEECCCCCCCEEEEEEECCCCEEEEECCCCEEECCCCCCCCCCC
PLADYATLANLLQPCAAAAVAAVGAPYLSALPVGLTQSIRVQRCATLAAAGLVSGADTAS
CHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCHH
QANDALAQLHAAGYLADSDLLQAPMWDSQAIPAIAVTYANAYTRSRVTDNLCNFSFATTN
HHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCEEEECC
SVTGAVAPPATSPMTSLFGAGNGVPPTNGINLVFNGASGGVDHRLATPDASFAGAFCLRQ
CCCCCCCCCCCCHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCEECCCCCHHHHHHHHHH
LWTANQLGIGANVDAVRVAANLQRKPAIIVQGRSDALVPVNHASRAYVAQNSATEGRASQ
HHCCCCCCCCCCCHHHHHHHCCCCCCEEEEECCCCCEEECCCCCCEEEECCCCCCCCHHE
LSFYEVTNGQHFDAFLSVPGFDTRFVPVHYYDEQALNLMWNHLKSGAPLPPSQVIRTVPR
EEEEEECCCCCEEEEEECCCCCCEEEEEEECCHHHHHHHHHHHHCCCCCCHHHHHHHCCC
GGVPGAAPALSTANLPPIVQSPGSNAITVNAGVIDVPL
CCCCCCCCCCCCCCCCCCEECCCCCEEEEECCEEECCC
>Mature Secondary Structure 
TTIRGGSRRASLPALALLGVLLGACHSDDNAQVNTLPGFVSGNVRKTAYDGASDDLLTA
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCEECCCEEEEECCCCCCCEEEE
GLGKTGLGSDTRPGFANPAQPTAAELRRLAIYSNYRALVDITPNGGYGRFWGPNVDLAGN
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHEECCEEEEEECCCCCCCCCCCCCCCCCCC
DTLGEGKIAGTEYLAYSDDGSGRKNVTLLVQVPASFDPANPCIVTATSSGSRGVYGAIAA
CCCCCCCCCCCEEEEECCCCCCCCCEEEEEEECCCCCCCCCEEEEEECCCCCCCEEEEEE
AGEWGLKRGCAVAYNDKGGGNGAHEIGTGVVTLIDGTLASASSAGSASLFTASESSSALA
CCCCCCCCCCEEEECCCCCCCCHHHHCCCEEEEEECCHHCCCCCCCEEEEEECCCCCHHH
AFNSAFPNRYAYKHAHSQQNPEQDWGRVTLQAVEFAYWALNEQFGPAIDGARHGVRYRPG
HHHHCCCCCHHHHHHHCCCCCCHHHHHEEHHHHHHHHHHHCCCCCCCCCHHHCCCEECCC
DITTIAASVSNGGAAALAAAEQDTRGWITAVVVGEPQINVRMTPGVTVEQGGVPAPSFGR
CEEEEEEECCCCCEEEEEECCCCCCCEEEEEEECCCCEEEEECCCCEEECCCCCCCCCCC
PLADYATLANLLQPCAAAAVAAVGAPYLSALPVGLTQSIRVQRCATLAAAGLVSGADTAS
CHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCHH
QANDALAQLHAAGYLADSDLLQAPMWDSQAIPAIAVTYANAYTRSRVTDNLCNFSFATTN
HHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCEEEECC
SVTGAVAPPATSPMTSLFGAGNGVPPTNGINLVFNGASGGVDHRLATPDASFAGAFCLRQ
CCCCCCCCCCCCHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCEECCCCCHHHHHHHHHH
LWTANQLGIGANVDAVRVAANLQRKPAIIVQGRSDALVPVNHASRAYVAQNSATEGRASQ
HHCCCCCCCCCCCHHHHHHHCCCCCCEEEEECCCCCEEECCCCCCEEEECCCCCCCCHHE
LSFYEVTNGQHFDAFLSVPGFDTRFVPVHYYDEQALNLMWNHLKSGAPLPPSQVIRTVPR
EEEEEECCCCCEEEEEECCCCCCEEEEEEECCHHHHHHHHHHHHCCCCCCHHHHHHHCCC
GGVPGAAPALSTANLPPIVQSPGSNAITVNAGVIDVPL
CCCCCCCCCCCCCCCCCCEECCCCCEEEEECCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA