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The map label for this gene is gsiB [H]

Identifier: 83593809

GI number: 83593809

Start: 2873323

End: 2874900

Strand: Direct

Name: gsiB [H]

Synonym: Rru_A2474

Alternate gene names: 83593809

Gene position: 2873323-2874900 (Clockwise)

Preceding gene: 83593808

Following gene: 83593810

Centisome position: 66.01

GC content: 70.6

Gene sequence:

>1578_bases
ATGACGGCCTGGATAGTCCTGACGGCCCCCGCCGCTTCGACCGCCGCCGAGTTCCGCTGGACGGCGGCGCAGGGCCCGGC
GACGCTGGAGCCCCAATCCCCGGAAGCCCTGGCCGACCCCGGGGTTCTGGGCGACGTTTATGAAACGCTGATCGGCCGCG
ACTCCGATCTTGACCTTGAACCGGCCCTGGCGACCGCTTGGCAGGCGGTGACCGATTTACGCTGGCGGGTCACCCTGCGT
CGGGGCGTGCGCTTCCATGACGGCGCCGCCTTCACCGCCGATGACGTGGTCGCCTCGCTGGAGCGCGCCAGCGCCCGCGG
CGGCGGTCTGGCCGAAGCGCTGGCGCCGGTGCGCCGCGTCCGCCGGGTCGATGATACGACGGTCGATCTTTACACCCAAA
CGCCCACCCCCGATCTGCCCCAACGCCTCGCCCTGATCAGAATCCTCGACGACGGCTGGATCGCCCGCGCCGATCCGGCG
ATGCCGGCCAATGGCACCGGCCCCTTCCGAGTGGACCGCTTCACCCCCGGCGGCGCCGTGACGCTGGTGGCCAATGGCGA
CTGGTGGGGACGGACGATCCCGGCGGACCCGCTGGGGACGACGGCGCCGCCCGCTCCACGGCTTGAGCGCGCCACCCTGA
TTCCCGCCGCCCGCCCGGCCGATCGCTTGCGGCTTTTGCTTGAAGGCAAGGTGGATCTGGCGCTGGATCTGCCGCCCGCC
GTGGTGCCTCCCCTGGCCGCCACCCCCGGCCTGCGCGCCCTGGTCATCGGCGGAACGCGCACGGTCATGCTGGGGATGGA
CCAGCGCCCGCCGCCCTTGGGCGCCGCTCGCGGCCAGGGCTCGCCCTTTCGCGACCGGCTGTTGCGCGAGGCGGTGCTGC
GCGCCGTTGATATGACCGACATCGACCAGCGCCTTTTCGCCAATCAGGCGACCCCGGCCGCCCTGATCGCCGGGCCGATG
ATCGCCGGGGTGCCCGCCGCCGCCGATATCCGCCCGGCCGCCGACCCAGACCGGGCCCGCGCCCTGCTTGCCGAAGCCGG
AGTCGGCCCCGCCGGAGTCAGCGTCACCCTGGATTGCCCGCTGGGCTTCTTCCTGAACGACGGCGCCCTTTGCGACGCCA
TCGCCACCTCTCTTTCGGAAGTCGGCATTCATACCGCCGTCGCCACCCGTTTGGCCGAAAATCATTTCCCGAGGGTTTTA
CGCGGGGAAAGCCGGTTCTTCCTAACCGGATACCAACCGTTGACCTTGGATATCCTCGATCCCTTGCGCGCCCTGGCCGC
TTGTCCGCCGACCGATGGCTCGCCGAAGGACGGCTTTGGCCAAGCCAATGGCGCGGGCTATTGCGACCCCTCCGTCGATC
GGCTTATCCGCCGTCTGGCCGACGAAATGATCCCGGCCCGCCAAAGCGCCCTTGCCGCCGAAATCGTCCTCAAACTGCGC
GATGACGTCGTCTATGTCCCCCTCCACCAGGAACCGGTGATCTGGGGGGCGCGGGCCGATATCGGTCTGCGCCAGCGCGC
CGACGGCGTGCTTGATCTCCGCTGGGTCAGCCCGGCGCCCGTCCCGCAAGGGCGCTGA

Upstream 100 bases:

>100_bases
AGGGCGCGATCTTTTTGATAAGGGTCCCGCGCCTTTCGGCCGGCCTTCCCGAGGGATGACGGTGGCCCGGGCGGCAAGGC
GCCAAAGGATGGCGCTGCTG

Downstream 100 bases:

>100_bases
TATCCCCCCCGCGATCCCGGCTCTCGCGACCGCGCCGCTAAAAACCAAGCAAAACAGTCGATTACACTCCCCCCTCGCCG
ACCCCAGCGGCGCCCGCCCG

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 525; Mature: 524

Protein sequence:

>525_residues
MTAWIVLTAPAASTAAEFRWTAAQGPATLEPQSPEALADPGVLGDVYETLIGRDSDLDLEPALATAWQAVTDLRWRVTLR
RGVRFHDGAAFTADDVVASLERASARGGGLAEALAPVRRVRRVDDTTVDLYTQTPTPDLPQRLALIRILDDGWIARADPA
MPANGTGPFRVDRFTPGGAVTLVANGDWWGRTIPADPLGTTAPPAPRLERATLIPAARPADRLRLLLEGKVDLALDLPPA
VVPPLAATPGLRALVIGGTRTVMLGMDQRPPPLGAARGQGSPFRDRLLREAVLRAVDMTDIDQRLFANQATPAALIAGPM
IAGVPAAADIRPAADPDRARALLAEAGVGPAGVSVTLDCPLGFFLNDGALCDAIATSLSEVGIHTAVATRLAENHFPRVL
RGESRFFLTGYQPLTLDILDPLRALAACPPTDGSPKDGFGQANGAGYCDPSVDRLIRRLADEMIPARQSALAAEIVLKLR
DDVVYVPLHQEPVIWGARADIGLRQRADGVLDLRWVSPAPVPQGR

Sequences:

>Translated_525_residues
MTAWIVLTAPAASTAAEFRWTAAQGPATLEPQSPEALADPGVLGDVYETLIGRDSDLDLEPALATAWQAVTDLRWRVTLR
RGVRFHDGAAFTADDVVASLERASARGGGLAEALAPVRRVRRVDDTTVDLYTQTPTPDLPQRLALIRILDDGWIARADPA
MPANGTGPFRVDRFTPGGAVTLVANGDWWGRTIPADPLGTTAPPAPRLERATLIPAARPADRLRLLLEGKVDLALDLPPA
VVPPLAATPGLRALVIGGTRTVMLGMDQRPPPLGAARGQGSPFRDRLLREAVLRAVDMTDIDQRLFANQATPAALIAGPM
IAGVPAAADIRPAADPDRARALLAEAGVGPAGVSVTLDCPLGFFLNDGALCDAIATSLSEVGIHTAVATRLAENHFPRVL
RGESRFFLTGYQPLTLDILDPLRALAACPPTDGSPKDGFGQANGAGYCDPSVDRLIRRLADEMIPARQSALAAEIVLKLR
DDVVYVPLHQEPVIWGARADIGLRQRADGVLDLRWVSPAPVPQGR
>Mature_524_residues
TAWIVLTAPAASTAAEFRWTAAQGPATLEPQSPEALADPGVLGDVYETLIGRDSDLDLEPALATAWQAVTDLRWRVTLRR
GVRFHDGAAFTADDVVASLERASARGGGLAEALAPVRRVRRVDDTTVDLYTQTPTPDLPQRLALIRILDDGWIARADPAM
PANGTGPFRVDRFTPGGAVTLVANGDWWGRTIPADPLGTTAPPAPRLERATLIPAARPADRLRLLLEGKVDLALDLPPAV
VPPLAATPGLRALVIGGTRTVMLGMDQRPPPLGAARGQGSPFRDRLLREAVLRAVDMTDIDQRLFANQATPAALIAGPMI
AGVPAAADIRPAADPDRARALLAEAGVGPAGVSVTLDCPLGFFLNDGALCDAIATSLSEVGIHTAVATRLAENHFPRVLR
GESRFFLTGYQPLTLDILDPLRALAACPPTDGSPKDGFGQANGAGYCDPSVDRLIRRLADEMIPARQSALAAEIVLKLRD
DVVYVPLHQEPVIWGARADIGLRQRADGVLDLRWVSPAPVPQGR

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=491, Percent_Identity=25.2545824847251, Blast_Score=96, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 55715; Mature: 55584

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAWIVLTAPAASTAAEFRWTAAQGPATLEPQSPEALADPGVLGDVYETLIGRDSDLDLE
CEEEEEEECCCCCCHHHEEEEECCCCCCCCCCCCHHHCCCCCHHHHHHHHHCCCCCCCCC
PALATAWQAVTDLRWRVTLRRGVRFHDGAAFTADDVVASLERASARGGGLAEALAPVRRV
HHHHHHHHHHHHHHEEEEHHHCCEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHH
RRVDDTTVDLYTQTPTPDLPQRLALIRILDDGWIARADPAMPANGTGPFRVDRFTPGGAV
HHCCCCEEEEEECCCCCCHHHHEEEEEEECCCCEEECCCCCCCCCCCCEEEEEECCCCEE
TLVANGDWWGRTIPADPLGTTAPPAPRLERATLIPAARPADRLRLLLEGKVDLALDLPPA
EEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHEEEEECCCEEEEEECCCC
VVPPLAATPGLRALVIGGTRTVMLGMDQRPPPLGAARGQGSPFRDRLLREAVLRAVDMTD
CCCCCCCCCCCEEEEECCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCH
IDQRLFANQATPAALIAGPMIAGVPAAADIRPAADPDRARALLAEAGVGPAGVSVTLDCP
HHHHHHHCCCCCHHHHHCHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECC
LGFFLNDGALCDAIATSLSEVGIHTAVATRLAENHFPRVLRGESRFFLTGYQPLTLDILD
CCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEECCCCEEHHHHH
PLRALAACPPTDGSPKDGFGQANGAGYCDPSVDRLIRRLADEMIPARQSALAAEIVLKLR
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DDVVYVPLHQEPVIWGARADIGLRQRADGVLDLRWVSPAPVPQGR
CCEEEEECCCCCEEEECCCCCCCHHCCCCEEEEEECCCCCCCCCC
>Mature Secondary Structure 
TAWIVLTAPAASTAAEFRWTAAQGPATLEPQSPEALADPGVLGDVYETLIGRDSDLDLE
EEEEEEECCCCCCHHHEEEEECCCCCCCCCCCCHHHCCCCCHHHHHHHHHCCCCCCCCC
PALATAWQAVTDLRWRVTLRRGVRFHDGAAFTADDVVASLERASARGGGLAEALAPVRRV
HHHHHHHHHHHHHHEEEEHHHCCEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHH
RRVDDTTVDLYTQTPTPDLPQRLALIRILDDGWIARADPAMPANGTGPFRVDRFTPGGAV
HHCCCCEEEEEECCCCCCHHHHEEEEEEECCCCEEECCCCCCCCCCCCEEEEEECCCCEE
TLVANGDWWGRTIPADPLGTTAPPAPRLERATLIPAARPADRLRLLLEGKVDLALDLPPA
EEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHEEEEECCCEEEEEECCCC
VVPPLAATPGLRALVIGGTRTVMLGMDQRPPPLGAARGQGSPFRDRLLREAVLRAVDMTD
CCCCCCCCCCCEEEEECCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCH
IDQRLFANQATPAALIAGPMIAGVPAAADIRPAADPDRARALLAEAGVGPAGVSVTLDCP
HHHHHHHCCCCCHHHHHCHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECC
LGFFLNDGALCDAIATSLSEVGIHTAVATRLAENHFPRVLRGESRFFLTGYQPLTLDILD
CCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEECCCCEEHHHHH
PLRALAACPPTDGSPKDGFGQANGAGYCDPSVDRLIRRLADEMIPARQSALAAEIVLKLR
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DDVVYVPLHQEPVIWGARADIGLRQRADGVLDLRWVSPAPVPQGR
CCEEEEECCCCCEEEECCCCCCCHHCCCCEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA