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The map label for this gene is cheB3

Identifier: 83593658

GI number: 83593658

Start: 2693636

End: 2694748

Strand: Direct

Name: cheB3

Synonym: Rru_A2323

Alternate gene names: 83593658

Gene position: 2693636-2694748 (Clockwise)

Preceding gene: 83593657

Following gene: 83593659

Centisome position: 61.88

GC content: 65.41

Gene sequence:

>1113_bases
ATGACCAAAGTTCTGATTGTCGATGACTCGGCGCTCATGCGGCGTCATCTGAAGGAAATCCTCGAAGTCCACGGCGGCTT
CGAGGTGATGGTGGCGCGCAATGGCATCGAGGCGCTGGCCGGCCTGGAAAGCTTCGACCCCGATGTCATCACCCTTGATA
TCAACATGCCCGAGATGGATGGCCTGACCTGCCTGTCGCGGATCATGGCGACCAAGCCAAAACCGGTGGTGATGGTGTCG
TCTTTGACCGAAACCGGCGCCGAGGTCACCCTCGAGGCCCTGGCCTTGGGGGCGGTCGATTTCATCCACAAACCCGATGG
CACCATTTCCCTCAATGTCTCGCGCATCGAGCGCGAAATCCTCGACAAGGTCACCGCCGCCGCCAAGGCCCGTATTCGCC
GCTCGCTCGGATTGTCGAGCCGCTTGCGCACGGCCAGCGACCGCGGCGCCGCCAAGCGGACCCGTCCCGCCGACTCCTCC
CCCCTCCCCACCCTGTCCCTCACCGAGATCGGCGTGGTGCTGATCGGGGTTTCAACCGGTGGACCGGGAACCCTGGAAGA
GGTTCTGCCCGGTCTGTCCGCCGGCTTTCCCTGGCCGGTGGTGGTGGCCCAGCACATGCCAGGCAGCTTCACCCCGGTGT
TCGCCCGCCGCCTCAATGACCTATGTCCGTTGACGGTGATCGAGGCCTCGGCGCAGATGCCGCTGGAACCCGGGGTGATC
TACATCGCCAAGGGCGACGCCGACGTGGTGATCGGCCATCGCGCCACCCGTTTTGTCGTCAATCCGATGCCCGCCGGCCC
CCATTATCTGTGGCATCCCAGCGTCACCCGCCTTGTCGAGAGCGCGCTGCGCGCCCTGCCGCCCGAGCGCATCATCGGCG
TGTTGTTGACCGGCATGGGAGATGACGGCGCCGAGGCGATGGCCGAGGTGAAGCGGCGCGGCGGGCGCACCATCGCCCAG
GACGAAGCCAGCTCCGTCATCTTTGGCATGCCCGGAGAGCTGGTCAAACGCGGCGGCGCCAATCTGGTGCTGCCGGCCAG
CCGCATCGCCGGTCAGCTCAATGCGTGGATGTCACGAACCAAGGAGACGGTCTATGGGACTGGTCAGAGCTGA

Upstream 100 bases:

>100_bases
ATTCTGATCTGCATTGACTGGGTTACAGCGGGAAAATCGCCGACACCGGCTATTTTTCCGCTCGCCCCTCCAGACCCGGC
TTTCATAAAGGCCCGCGGAC

Downstream 100 bases:

>100_bases
AACAGCGCGCGACGGCTCGTCCGGGCCCGCCGATAACCGCCACCTCACTGATTTGCTCAGCGATTTGGCGGCGCCGGCCC
CGGGCACCCGTCGGGCCGCG

Product: two component CheB methylesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 369

Protein sequence:

>370_residues
MTKVLIVDDSALMRRHLKEILEVHGGFEVMVARNGIEALAGLESFDPDVITLDINMPEMDGLTCLSRIMATKPKPVVMVS
SLTETGAEVTLEALALGAVDFIHKPDGTISLNVSRIEREILDKVTAAAKARIRRSLGLSSRLRTASDRGAAKRTRPADSS
PLPTLSLTEIGVVLIGVSTGGPGTLEEVLPGLSAGFPWPVVVAQHMPGSFTPVFARRLNDLCPLTVIEASAQMPLEPGVI
YIAKGDADVVIGHRATRFVVNPMPAGPHYLWHPSVTRLVESALRALPPERIIGVLLTGMGDDGAEAMAEVKRRGGRTIAQ
DEASSVIFGMPGELVKRGGANLVLPASRIAGQLNAWMSRTKETVYGTGQS

Sequences:

>Translated_370_residues
MTKVLIVDDSALMRRHLKEILEVHGGFEVMVARNGIEALAGLESFDPDVITLDINMPEMDGLTCLSRIMATKPKPVVMVS
SLTETGAEVTLEALALGAVDFIHKPDGTISLNVSRIEREILDKVTAAAKARIRRSLGLSSRLRTASDRGAAKRTRPADSS
PLPTLSLTEIGVVLIGVSTGGPGTLEEVLPGLSAGFPWPVVVAQHMPGSFTPVFARRLNDLCPLTVIEASAQMPLEPGVI
YIAKGDADVVIGHRATRFVVNPMPAGPHYLWHPSVTRLVESALRALPPERIIGVLLTGMGDDGAEAMAEVKRRGGRTIAQ
DEASSVIFGMPGELVKRGGANLVLPASRIAGQLNAWMSRTKETVYGTGQS
>Mature_369_residues
TKVLIVDDSALMRRHLKEILEVHGGFEVMVARNGIEALAGLESFDPDVITLDINMPEMDGLTCLSRIMATKPKPVVMVSS
LTETGAEVTLEALALGAVDFIHKPDGTISLNVSRIEREILDKVTAAAKARIRRSLGLSSRLRTASDRGAAKRTRPADSSP
LPTLSLTEIGVVLIGVSTGGPGTLEEVLPGLSAGFPWPVVVAQHMPGSFTPVFARRLNDLCPLTVIEASAQMPLEPGVIY
IAKGDADVVIGHRATRFVVNPMPAGPHYLWHPSVTRLVESALRALPPERIIGVLLTGMGDDGAEAMAEVKRRGGRTIAQD
EASSVIFGMPGELVKRGGANLVLPASRIAGQLNAWMSRTKETVYGTGQS

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1788192, Length=358, Percent_Identity=39.6648044692737, Blast_Score=205, Evalue=4e-54,
Organism=Escherichia coli, GI1788550, Length=114, Percent_Identity=36.8421052631579, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CHEB3_RHORT (Q2RRX2)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_427410.1
- ProteinModelPortal:   Q2RRX2
- STRING:   Q2RRX2
- GeneID:   3835751
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A2323
- NMPDR:   fig|1085.1.peg.3799
- eggNOG:   COG2201
- HOGENOM:   HBG705324
- OMA:   PADFPWP
- PhylomeDB:   Q2RRX2
- ProtClustDB:   CLSK943768
- BioCyc:   RRUB269796:RRU_A2323-MONOMER
- GO:   GO:0005737
- GO:   GO:0006935
- HAMAP:   MF_00099
- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789
- Gene3D:   G3DSA:3.40.50.180
- PIRSF:   PIRSF000876
- SMART:   SM00448

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg; SSF52738 Chemotax_RR_pGlu_Me-esterase; SSF52172 CheY_like

EC number: =3.1.1.61

Molecular weight: Translated: 39398; Mature: 39267

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: PS50122 CHEB; PS50110 RESPONSE_REGULATORY; PS00120 LIPASE_SER

Important sites: ACT_SITE 178-178 ACT_SITE 205-205 ACT_SITE 302-302

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKVLIVDDSALMRRHLKEILEVHGGFEVMVARNGIEALAGLESFDPDVITLDINMPEMD
CCEEEEECCHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHCCCCCCCCEEEEEECCCCCC
GLTCLSRIMATKPKPVVMVSSLTETGAEVTLEALALGAVDFIHKPDGTISLNVSRIEREI
HHHHHHHHHHCCCCCEEEEECHHHCCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHH
LDKVTAAAKARIRRSLGLSSRLRTASDRGAAKRTRPADSSPLPTLSLTEIGVVLIGVSTG
HHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCEEEEEECCC
GPGTLEEVLPGLSAGFPWPVVVAQHMPGSFTPVFARRLNDLCPLTVIEASAQMPLEPGVI
CCCCHHHHCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCEE
YIAKGDADVVIGHRATRFVVNPMPAGPHYLWHPSVTRLVESALRALPPERIIGVLLTGMG
EEECCCCEEEECCCCEEEEECCCCCCCCEEECCHHHHHHHHHHHHCCHHHHHHHHHCCCC
DDGAEAMAEVKRRGGRTIAQDEASSVIFGMPGELVKRGGANLVLPASRIAGQLNAWMSRT
CCHHHHHHHHHHHCCCEEECCCCCEEEECCCHHHHHCCCCCEEEEHHHHHHHHHHHHHHH
KETVYGTGQS
HHHEECCCCC
>Mature Secondary Structure 
TKVLIVDDSALMRRHLKEILEVHGGFEVMVARNGIEALAGLESFDPDVITLDINMPEMD
CEEEEECCHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHCCCCCCCCEEEEEECCCCCC
GLTCLSRIMATKPKPVVMVSSLTETGAEVTLEALALGAVDFIHKPDGTISLNVSRIEREI
HHHHHHHHHHCCCCCEEEEECHHHCCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHH
LDKVTAAAKARIRRSLGLSSRLRTASDRGAAKRTRPADSSPLPTLSLTEIGVVLIGVSTG
HHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCEEEEEECCC
GPGTLEEVLPGLSAGFPWPVVVAQHMPGSFTPVFARRLNDLCPLTVIEASAQMPLEPGVI
CCCCHHHHCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCEE
YIAKGDADVVIGHRATRFVVNPMPAGPHYLWHPSVTRLVESALRALPPERIIGVLLTGMG
EEECCCCEEEECCCCEEEEECCCCCCCCEEECCHHHHHHHHHHHHCCHHHHHHHHHCCCC
DDGAEAMAEVKRRGGRTIAQDEASSVIFGMPGELVKRGGANLVLPASRIAGQLNAWMSRT
CCHHHHHHHHHHHCCCEEECCCCCEEEECCCHHHHHCCCCCEEEEHHHHHHHHHHHHHHH
KETVYGTGQS
HHHEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA