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The map label for this gene is artI [H]

Identifier: 83593586

GI number: 83593586

Start: 2613579

End: 2614370

Strand: Direct

Name: artI [H]

Synonym: Rru_A2251

Alternate gene names: 83593586

Gene position: 2613579-2614370 (Clockwise)

Preceding gene: 83593585

Following gene: 83593587

Centisome position: 60.04

GC content: 61.87

Gene sequence:

>792_bases
ATGATCAGCATGAAGAAGACAGCGACCGCGTTGTTCGCCGCCGCCGGGATCCTCCTGGGCGGCCAGGCCGTCACCATGGC
CCTGGGCGTTGCCGACGCCCGGGCGGAAACCATCCGCTTCGCCACCGAAGGCGCCTTCCCGCCGTTCAACTTCACCGATG
CCGCCGGCAAGCCCCAGGGCTTCGATATCGACATCGCCAATGCGCTGTGCGCCCAGTTGAAGGCCGAGTGCAGCATGGTC
ACCCAGGATTGGGATGGCATCATTCCCGGACTGCTGGCCAAGAAGTACGACGCCATCATCGCCTCGATGTCGATCACCCC
CGAGCGTCAGCAGAGCGTCTTGTTCACCGACGCCTATTATAACAACCAGTTCCATTTCGTCGGCCCGAAGGGCAAGGATT
TCGATCTGAGCAAGGCCGGCCTGAAGGGCAAGGCCATCGGCGCCCAGCGCGCCACCGTCTCCTCGCAGTGGATCGAGGAC
ACCCTTGGCGACACCGTCAGCCTCAACCTTTATGACACCGCCGAAGAGGCCTATCTCGACCTCGAAGCCGGCCGCGTCGA
TTTGCTGCTCAACGACACCTATCCCACCTACGACTGGCTCAACAGCCCGGCCGGCCAAGCCTATGAACTCAAGGGCGAAT
CGGTGATCAAGGACGACGTCGTCGGCATCGCCTTGCGCAAGGACGACACCGCCCTGGCCGCCAAGCTCAATGGCGCGCTC
AAGGAAATCATCGCCAATGGCACCTATGCCAAGATCAACGAGAAGTACTTCCCCTTCAGCCTGCTGAACTAG

Upstream 100 bases:

>100_bases
GGCCGGGCATCCCGTCGGGGGACGGGGAAACACCCCGGCTCCCGTCCAAAGGGGTCCGCCGTCCCGGGCTCCACCAGACA
GGCACAGGAAAACGACGACC

Downstream 100 bases:

>100_bases
GGTTAGGGGTAGAAGACCTTTCGGGGGGCGCTCCCCGGGAAGACGGTGGGTGTGATCCGGGCGTAAGGCTTGGGGATCGC
GCCCTGAACCGGCCCGGGGA

Product: extracellular solute-binding protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MISMKKTATALFAAAGILLGGQAVTMALGVADARAETIRFATEGAFPPFNFTDAAGKPQGFDIDIANALCAQLKAECSMV
TQDWDGIIPGLLAKKYDAIIASMSITPERQQSVLFTDAYYNNQFHFVGPKGKDFDLSKAGLKGKAIGAQRATVSSQWIED
TLGDTVSLNLYDTAEEAYLDLEAGRVDLLLNDTYPTYDWLNSPAGQAYELKGESVIKDDVVGIALRKDDTALAAKLNGAL
KEIIANGTYAKINEKYFPFSLLN

Sequences:

>Translated_263_residues
MISMKKTATALFAAAGILLGGQAVTMALGVADARAETIRFATEGAFPPFNFTDAAGKPQGFDIDIANALCAQLKAECSMV
TQDWDGIIPGLLAKKYDAIIASMSITPERQQSVLFTDAYYNNQFHFVGPKGKDFDLSKAGLKGKAIGAQRATVSSQWIED
TLGDTVSLNLYDTAEEAYLDLEAGRVDLLLNDTYPTYDWLNSPAGQAYELKGESVIKDDVVGIALRKDDTALAAKLNGAL
KEIIANGTYAKINEKYFPFSLLN
>Mature_263_residues
MISMKKTATALFAAAGILLGGQAVTMALGVADARAETIRFATEGAFPPFNFTDAAGKPQGFDIDIANALCAQLKAECSMV
TQDWDGIIPGLLAKKYDAIIASMSITPERQQSVLFTDAYYNNQFHFVGPKGKDFDLSKAGLKGKAIGAQRATVSSQWIED
TLGDTVSLNLYDTAEEAYLDLEAGRVDLLLNDTYPTYDWLNSPAGQAYELKGESVIKDDVVGIALRKDDTALAAKLNGAL
KEIIANGTYAKINEKYFPFSLLN

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787088, Length=228, Percent_Identity=40.7894736842105, Blast_Score=167, Evalue=7e-43,
Organism=Escherichia coli, GI1787085, Length=226, Percent_Identity=40.2654867256637, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1788649, Length=265, Percent_Identity=36.9811320754717, Blast_Score=155, Evalue=2e-39,
Organism=Escherichia coli, GI1788648, Length=237, Percent_Identity=35.0210970464135, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1788228, Length=222, Percent_Identity=30.1801801801802, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1787031, Length=237, Percent_Identity=32.0675105485232, Blast_Score=104, Evalue=6e-24,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28327; Mature: 28327

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISMKKTATALFAAAGILLGGQAVTMALGVADARAETIRFATEGAFPPFNFTDAAGKPQG
CCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHEEEEEECCCCCCCCCCCCCCCCCC
FDIDIANALCAQLKAECSMVTQDWDGIIPGLLAKKYDAIIASMSITPERQQSVLFTDAYY
CEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEEECCCCHHCEEEEEEEEE
NNQFHFVGPKGKDFDLSKAGLKGKAIGAQRATVSSQWIEDTLGDTVSLNLYDTAEEAYLD
CCEEEEECCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCHHEEE
LEAGRVDLLLNDTYPTYDWLNSPAGQAYELKGESVIKDDVVGIALRKDDTALAAKLNGAL
ECCCEEEEEECCCCCCHHHCCCCCCCEEEECCCCEECCCEEEEEEECCCCHHHHHHHHHH
KEIIANGTYAKINEKYFPFSLLN
HHHHCCCCEEEECCCCCCEEECC
>Mature Secondary Structure
MISMKKTATALFAAAGILLGGQAVTMALGVADARAETIRFATEGAFPPFNFTDAAGKPQG
CCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHEEEEEECCCCCCCCCCCCCCCCCC
FDIDIANALCAQLKAECSMVTQDWDGIIPGLLAKKYDAIIASMSITPERQQSVLFTDAYY
CEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEEECCCCHHCEEEEEEEEE
NNQFHFVGPKGKDFDLSKAGLKGKAIGAQRATVSSQWIEDTLGDTVSLNLYDTAEEAYLD
CCEEEEECCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCHHEEE
LEAGRVDLLLNDTYPTYDWLNSPAGQAYELKGESVIKDDVVGIALRKDDTALAAKLNGAL
ECCCEEEEEECCCCCCHHHCCCCCCCEEEECCCCEECCCEEEEEEECCCCHHHHHHHHHH
KEIIANGTYAKINEKYFPFSLLN
HHHHCCCCEEEECCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]