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The map label for this gene is mttC2 [H]

Identifier: 83590291

GI number: 83590291

Start: 1489248

End: 1490918

Strand: Reverse

Name: mttC2 [H]

Synonym: Moth_1447

Alternate gene names: 83590291

Gene position: 1490918-1489248 (Counterclockwise)

Preceding gene: 83590292

Following gene: 83590290

Centisome position: 56.72

GC content: 52.78

Gene sequence:

>1671_bases
ATGCGGCTCATTGACCTGGTGAATCAATCCGGGCGGCATCTGGTATTTTTCATTGGCAGTTTTCCTGGTGCAGCCATGAA
AGGCGTTACATTGAAAGAAGTATACTTTTCCGCTTCTCTCCAGGCAGAAACGGCCTGCTACCTGGCGGAACGTTTCCAGA
TCGATTTTATCCGTCCGGTTACTGACCTGGTGGTAGAGGCTGAGGCTATGGGCTTAAAGGCGCGTTATCCAGATAATGGC
GCCCCGGTGCTGGTAGAGCATCCAATAACTGGACCAGAGGCGCTGGCCAACTTAAACTTACCGGAACCCGGCCGTGACGG
CCGTCTCCCGATAAATCTTGAAGCCATTCGCTTGATAAAGCAGCGGACCGACAAACCGGTGATAGGTTCCTTGACAGGCC
CTTTTACTTTAGCCGGTGCCCTCTGCGATCCGGCTGGCGTGGCCATGAAAACAATTACTGACCCCGAGTTTCTGCATGCT
CTCCTGGCCTTTTGTACCCGGGCTTTGAAACGATACGGGGAAGCCCTGCTGGCAGCGGGGGCGGATATCCTCTGGATTTC
TGAACCCCTGGGCTCTTTGCTGTCACCAGCACAGTTCTGGCAGTTTTCCGGCCGGTATATCCAGGAGATTTTTGCGGCTT
TCCCGGCCATGGATATACTCCATATCTGTGGCGATACCAGTTACATGATTAAAGAAATGCTGGCCACAGGCGCCCAGGGT
TTGAGCCTGGACAGCAGGGTTAGTTTACCTGTCCTGGCAACCCAAATGCCGGAGGATGTAGTTCTAATCGGTAATATCGA
TCCCGTCGGGGTGATGCTGGAAGGGTCACCCCAGCAGGTAGTAAAAGCTACTGCTAACCTGCTAACGGCTATGCTGCCGT
TCAACAACTTTATTGTGAGTACGGGATGCACTTTGCCCTTTGAAGTCCCGGCAGCGAATATTAGTGCTTTTGTGGAAACA
GCCAGGAACTTTCCCCGTTTATCTCCCTCCCAGGCCCGGTTGTTGCTTTCTTTACGACGAGCTTTGCTTGAAGGCGATAG
TGAAGGAGTAACAACGCTGACCAGGAAAGGGTTGCAACTGGAGGTGGATGCAATTACCATTCTCGAGGGAGGCCTGATCC
AGGGAATAAACCGGGCCGGGGAATTATACCAGCAACAGAAAGTATTTATCCCTGAGCTCTTGTTGATTAGCCAGGCCATG
TACGCCGGCCTGGAGGTTATCAGGCCGGTACTGGTGCAAAAGCGGCATGGCACCCGGGGCGAGATTGTGCTGGGAACTGT
TCAGGGAGACCTGCACGATATTGGTAAAAATCTGGTTGGTTTGATGTTAACAGCTAATGATTATGAGGTTATCGACCTGG
GTAAAGATGTAGCCCCGGAGCAGTTTGTTGAAGCTGTACGGGCATGCCGGCCCCAGGTAGTGGGTCTGTCGGCTTTGACC
ACCACAACCATGAAGTCCATGGAAAAGACCGTGCGGGCGTTAAAACAAGGGGGTCAAGGGGAGCAAGTTAAAGTTATAGT
GGGAGGAGCCCCCATTACCCCCGAGTTTGCCCGGCGGATTGGCGCTGATGCCTATGCTCCCGATGCTGCGGCGGCGGTTA
CTGAGGTGGCTAATTTAATAGATAAGGTGAAGGAGCATCAAGATGGCCAATCAACCACTGGCGTATTTTGA

Upstream 100 bases:

>100_bases
ATTGTACAGTCATAGCAATTCCCCTGGGGATATTAATGGTGGTTATCAGGTGATAAAAGCCTGATCGGCTCGATTGAATA
AACGGTGTGGGGGTAAAGAC

Downstream 100 bases:

>100_bases
TGATTTGCGGGAAAGAAATGTCCTGGAAATCAAAAAGTTGAAAGACCAGGGGAAAAAGGTAGTTGGCACTTATTGTGCCT
TTACCCCCAAGGAACTCATC

Product: cobalamin B12-binding

Products: L-Methionine; 5,6,7,8-Tetrahydrofolate [C]

Alternate protein names: TCP 2 [H]

Number of amino acids: Translated: 556; Mature: 556

Protein sequence:

>556_residues
MRLIDLVNQSGRHLVFFIGSFPGAAMKGVTLKEVYFSASLQAETACYLAERFQIDFIRPVTDLVVEAEAMGLKARYPDNG
APVLVEHPITGPEALANLNLPEPGRDGRLPINLEAIRLIKQRTDKPVIGSLTGPFTLAGALCDPAGVAMKTITDPEFLHA
LLAFCTRALKRYGEALLAAGADILWISEPLGSLLSPAQFWQFSGRYIQEIFAAFPAMDILHICGDTSYMIKEMLATGAQG
LSLDSRVSLPVLATQMPEDVVLIGNIDPVGVMLEGSPQQVVKATANLLTAMLPFNNFIVSTGCTLPFEVPAANISAFVET
ARNFPRLSPSQARLLLSLRRALLEGDSEGVTTLTRKGLQLEVDAITILEGGLIQGINRAGELYQQQKVFIPELLLISQAM
YAGLEVIRPVLVQKRHGTRGEIVLGTVQGDLHDIGKNLVGLMLTANDYEVIDLGKDVAPEQFVEAVRACRPQVVGLSALT
TTTMKSMEKTVRALKQGGQGEQVKVIVGGAPITPEFARRIGADAYAPDAAAAVTEVANLIDKVKEHQDGQSTTGVF

Sequences:

>Translated_556_residues
MRLIDLVNQSGRHLVFFIGSFPGAAMKGVTLKEVYFSASLQAETACYLAERFQIDFIRPVTDLVVEAEAMGLKARYPDNG
APVLVEHPITGPEALANLNLPEPGRDGRLPINLEAIRLIKQRTDKPVIGSLTGPFTLAGALCDPAGVAMKTITDPEFLHA
LLAFCTRALKRYGEALLAAGADILWISEPLGSLLSPAQFWQFSGRYIQEIFAAFPAMDILHICGDTSYMIKEMLATGAQG
LSLDSRVSLPVLATQMPEDVVLIGNIDPVGVMLEGSPQQVVKATANLLTAMLPFNNFIVSTGCTLPFEVPAANISAFVET
ARNFPRLSPSQARLLLSLRRALLEGDSEGVTTLTRKGLQLEVDAITILEGGLIQGINRAGELYQQQKVFIPELLLISQAM
YAGLEVIRPVLVQKRHGTRGEIVLGTVQGDLHDIGKNLVGLMLTANDYEVIDLGKDVAPEQFVEAVRACRPQVVGLSALT
TTTMKSMEKTVRALKQGGQGEQVKVIVGGAPITPEFARRIGADAYAPDAAAAVTEVANLIDKVKEHQDGQSTTGVF
>Mature_556_residues
MRLIDLVNQSGRHLVFFIGSFPGAAMKGVTLKEVYFSASLQAETACYLAERFQIDFIRPVTDLVVEAEAMGLKARYPDNG
APVLVEHPITGPEALANLNLPEPGRDGRLPINLEAIRLIKQRTDKPVIGSLTGPFTLAGALCDPAGVAMKTITDPEFLHA
LLAFCTRALKRYGEALLAAGADILWISEPLGSLLSPAQFWQFSGRYIQEIFAAFPAMDILHICGDTSYMIKEMLATGAQG
LSLDSRVSLPVLATQMPEDVVLIGNIDPVGVMLEGSPQQVVKATANLLTAMLPFNNFIVSTGCTLPFEVPAANISAFVET
ARNFPRLSPSQARLLLSLRRALLEGDSEGVTTLTRKGLQLEVDAITILEGGLIQGINRAGELYQQQKVFIPELLLISQAM
YAGLEVIRPVLVQKRHGTRGEIVLGTVQGDLHDIGKNLVGLMLTANDYEVIDLGKDVAPEQFVEAVRACRPQVVGLSALT
TTTMKSMEKTVRALKQGGQGEQVKVIVGGAPITPEFARRIGADAYAPDAAAAVTEVANLIDKVKEHQDGQSTTGVF

Specific function: Acts probably as a methyl group carrier between mttB and either mtbA or mtaA [H]

COG id: COG5012

COG function: function code R; Predicted cobalamin binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=197, Percent_Identity=28.9340101522843, Blast_Score=87, Evalue=4e-17,
Organism=Escherichia coli, GI1790450, Length=175, Percent_Identity=32, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI2367337, Length=318, Percent_Identity=24.8427672955975, Blast_Score=77, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17535405, Length=229, Percent_Identity=24.8908296943231, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR003759
- InterPro:   IPR012741 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2 [H]

EC number: 2.1.1.13 [C]

Molecular weight: Translated: 59806; Mature: 59806

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLIDLVNQSGRHLVFFIGSFPGAAMKGVTLKEVYFSASLQAETACYLAERFQIDFIRPV
CCCHHHHCCCCCEEEEEEECCCCCHHCCCCHHHHHHHCCCCHHHHHHHHHHHCCCHHHHH
TDLVVEAEAMGLKARYPDNGAPVLVEHPITGPEALANLNLPEPGRDGRLPINLEAIRLIK
HHHHHHHHHHCCEEECCCCCCCEEEECCCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHH
QRTDKPVIGSLTGPFTLAGALCDPAGVAMKTITDPEFLHALLAFCTRALKRYGEALLAAG
HHCCCCCEECCCCCHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCC
ADILWISEPLGSLLSPAQFWQFSGRYIQEIFAAFPAMDILHICGDTSYMIKEMLATGAQG
CCEEEECCHHHHHCCHHHHHHHCCHHHHHHHHHHCHHHHHHHHCCHHHHHHHHHHCCCCC
LSLDSRVSLPVLATQMPEDVVLIGNIDPVGVMLEGSPQQVVKATANLLTAMLPFNNFIVS
CCCCCCCCCCHHHHCCCCCEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEE
TGCTLPFEVPAANISAFVETARNFPRLSPSQARLLLSLRRALLEGDSEGVTTLTRKGLQL
CCCCCCEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCEE
EVDAITILEGGLIQGINRAGELYQQQKVFIPELLLISQAMYAGLEVIRPVLVQKRHGTRG
EEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
EIVLGTVQGDLHDIGKNLVGLMLTANDYEVIDLGKDVAPEQFVEAVRACRPQVVGLSALT
CEEEEECCCHHHHHHHHHEEEEEECCCEEEEECCCCCCHHHHHHHHHHCCCCCCCHHHHH
TTTMKSMEKTVRALKQGGQGEQVKVIVGGAPITPEFARRIGADAYAPDAAAAVTEVANLI
HHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHH
DKVKEHQDGQSTTGVF
HHHHHCCCCCCCCCCC
>Mature Secondary Structure
MRLIDLVNQSGRHLVFFIGSFPGAAMKGVTLKEVYFSASLQAETACYLAERFQIDFIRPV
CCCHHHHCCCCCEEEEEEECCCCCHHCCCCHHHHHHHCCCCHHHHHHHHHHHCCCHHHHH
TDLVVEAEAMGLKARYPDNGAPVLVEHPITGPEALANLNLPEPGRDGRLPINLEAIRLIK
HHHHHHHHHHCCEEECCCCCCCEEEECCCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHH
QRTDKPVIGSLTGPFTLAGALCDPAGVAMKTITDPEFLHALLAFCTRALKRYGEALLAAG
HHCCCCCEECCCCCHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCC
ADILWISEPLGSLLSPAQFWQFSGRYIQEIFAAFPAMDILHICGDTSYMIKEMLATGAQG
CCEEEECCHHHHHCCHHHHHHHCCHHHHHHHHHHCHHHHHHHHCCHHHHHHHHHHCCCCC
LSLDSRVSLPVLATQMPEDVVLIGNIDPVGVMLEGSPQQVVKATANLLTAMLPFNNFIVS
CCCCCCCCCCHHHHCCCCCEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEE
TGCTLPFEVPAANISAFVETARNFPRLSPSQARLLLSLRRALLEGDSEGVTTLTRKGLQL
CCCCCCEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCEE
EVDAITILEGGLIQGINRAGELYQQQKVFIPELLLISQAMYAGLEVIRPVLVQKRHGTRG
EEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
EIVLGTVQGDLHDIGKNLVGLMLTANDYEVIDLGKDVAPEQFVEAVRACRPQVVGLSALT
CEEEEECCCHHHHHHHHHEEEEEECCCEEEEECCCCCCHHHHHHHHHHCCCCCCCHHHHH
TTTMKSMEKTVRALKQGGQGEQVKVIVGGAPITPEFARRIGADAYAPDAAAAVTEVANLI
HHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHH
DKVKEHQDGQSTTGVF
HHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: ATP; Cobinamide; S-adenosyl-L-methionine [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 500-780 [C]

Specific activity: 3.8

Km value (mM): 0.0016 {S-adenosyl-L-methionine}} 0.00065 {S-adenosyl-L-methionine}} 0.06 {methyltetrahydrofolate}} 0.03 {methyltetrahydrofolate}} 0.016 {homocysteine}} 0.035 {S-methyltetrahydrofolate}} [C]

Substrates: 5-Methyltetrahydrofolate; L-Homocysteine [C]

Specific reaction: 5-Methyltetrahydrofolate + L-Homocysteine --> L-Methionine + 5,6,7,8-Tetrahydrofolate [C]

General reaction: Methyl group transfer [C]

Inhibitor: 5, 6-Dimethyl benzimidazole; alpha-Ribazole 3'-phosphate; Be2+; Cobinamide; Propyl iodide; Sr2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12125824 [H]