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The map label for this gene is radA

Identifier: 82750233

GI number: 82750233

Start: 534176

End: 535540

Strand: Direct

Name: radA

Synonym: SAB0476

Alternate gene names: 82750233

Gene position: 534176-535540 (Clockwise)

Preceding gene: 82750232

Following gene: 82750234

Centisome position: 19.48

GC content: 37.07

Gene sequence:

>1365_bases
TTGGCCAAGAAAAAAGTGATTTTTGAATGTATGGCTTGTGGTTATCAATCTCCTAAATGGATGGGGAAATGTCCTAATTG
TGGCGCTTGGAATCAAATGGAGGAAATTGTTGAAAAAGCAGCCAATCCTAAACATGGAGTGAAAACCAAGGAATTAGCAG
GTAAAGTACAAAAATTAAATAGTATTAAACATGAAACAACGCCGAGAGTGTTAACAGATTCAGCAGAATTCAACCGTGTA
TTAGGTGGAGGTATTGTGAGCGGATCGTTAGTACTTATTGGTGGGGATCCAGGTATTGGTAAGTCAACGTTACTTTTACA
AATTTGTGCATCGTTATCTCAAAAGAAAAAAGTACTATATATTACTGGAGAAGAATCGCTTAGTCAGACTAAATTACGTG
CAGAGCGATTAGATGAAGATTCAAGTGAATTGCAAGTATTAGCTGAAACAGATCTTGAAGTTATTTATCAAACAGTAAAA
GAAGAACAACCTGATTTATTAGTAGTGGATTCGATTCAAACAATATATCATCCTGAAATCAGCTCTGCGCCAGGTTCTGT
TTCACAAGTTCGTGAAAGTACACAAAGTTTAATGAATATTGCTAAACAAATGAACATTGCAACTTTTATAGTGGGTCATG
TAACGAAAGAAGGTCAAATTGCTGGCCCAAGATTGCTAGAACACATGGTTGATACTGTGCTTTATTTTGAAGGCGATGAA
CACCACGCATATCGAATTTTGCGAGCTGTTAAAAACCGTTTTGGTTCAACGAATGAAATGGGAATCTTCGAAATGAAGCA
AAGTGGATTAAAAGGTGTAAATAATCCATCTGAAATGTTTTTAGAAGAACGTTCAACAAATGTTCCAGGTTCAACAATTG
TTGCAACCATGGAGGGAACCAGACCACTTTTAATAGAAGTTCAAGCGCTGGTAACTCCAACGACTTTTAACAATCCGAGA
CGAATGGCAACAGGGATTGATCATAATCGATTAAGTTTGTTGATGGCTGTTTTGGAAAAGAAAGAAAATTATCTATTACA
ACAACAAGATGCTTATATCAAAGTAGCTGGCGGTGTAAAGTTAACGGAGCCAGCAGTTGATTTAAGTGTAATTGTAGCAA
CTGCGTCTAGCTTTAAAGATAAAGCTGTCGACGGCTTAGATTGCTATATTGGAGAAGTTGGTTTAACGGGTGAGGTACGT
CGTGTATCTCGGATAGAACAACGCGTGCAAGAGGCTGCAAAACTAGGTTTCAAACGTGTAATTATTCCTAAAAATAATAT
AGGCGGATGGACATATCCTGAAGGTATACAAGTAATAGGTGTAACTACTGTACATGAAGCATTGTCATTTGCTCTTCATT
CATAA

Upstream 100 bases:

>100_bases
GTCATGTTAAAATACAAACAAATGTAACTCACATTTAATTTGTCATAATGGGAATGTGCGTTTAAAATAGATTTGTTCAA
AGGAAAGTGGAGGTGCAATT

Downstream 100 bases:

>100_bases
AAAATCAAGAAAGGAGGACATTGTGTGAATATCGTTAAACTAATGGTTATTATTATTTACTTAATTATTGGGAGCGCATT
AGGAATAATTATTATTCCTG

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MAKKKVIFECMACGYQSPKWMGKCPNCGAWNQMEEIVEKAANPKHGVKTKELAGKVQKLNSIKHETTPRVLTDSAEFNRV
LGGGIVSGSLVLIGGDPGIGKSTLLLQICASLSQKKKVLYITGEESLSQTKLRAERLDEDSSELQVLAETDLEVIYQTVK
EEQPDLLVVDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGDE
HHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVNNPSEMFLEERSTNVPGSTIVATMEGTRPLLIEVQALVTPTTFNNPR
RMATGIDHNRLSLLMAVLEKKENYLLQQQDAYIKVAGGVKLTEPAVDLSVIVATASSFKDKAVDGLDCYIGEVGLTGEVR
RVSRIEQRVQEAAKLGFKRVIIPKNNIGGWTYPEGIQVIGVTTVHEALSFALHS

Sequences:

>Translated_454_residues
MAKKKVIFECMACGYQSPKWMGKCPNCGAWNQMEEIVEKAANPKHGVKTKELAGKVQKLNSIKHETTPRVLTDSAEFNRV
LGGGIVSGSLVLIGGDPGIGKSTLLLQICASLSQKKKVLYITGEESLSQTKLRAERLDEDSSELQVLAETDLEVIYQTVK
EEQPDLLVVDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGDE
HHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVNNPSEMFLEERSTNVPGSTIVATMEGTRPLLIEVQALVTPTTFNNPR
RMATGIDHNRLSLLMAVLEKKENYLLQQQDAYIKVAGGVKLTEPAVDLSVIVATASSFKDKAVDGLDCYIGEVGLTGEVR
RVSRIEQRVQEAAKLGFKRVIIPKNNIGGWTYPEGIQVIGVTTVHEALSFALHS
>Mature_453_residues
AKKKVIFECMACGYQSPKWMGKCPNCGAWNQMEEIVEKAANPKHGVKTKELAGKVQKLNSIKHETTPRVLTDSAEFNRVL
GGGIVSGSLVLIGGDPGIGKSTLLLQICASLSQKKKVLYITGEESLSQTKLRAERLDEDSSELQVLAETDLEVIYQTVKE
EQPDLLVVDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGDEH
HAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVNNPSEMFLEERSTNVPGSTIVATMEGTRPLLIEVQALVTPTTFNNPRR
MATGIDHNRLSLLMAVLEKKENYLLQQQDAYIKVAGGVKLTEPAVDLSVIVATASSFKDKAVDGLDCYIGEVGLTGEVRR
VSRIEQRVQEAAKLGFKRVIIPKNNIGGWTYPEGIQVIGVTTVHEALSFALHS

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=458, Percent_Identity=46.0698689956332, Blast_Score=414, Evalue=1e-117,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 49836; Mature: 49705

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKKVIFECMACGYQSPKWMGKCPNCGAWNQMEEIVEKAANPKHGVKTKELAGKVQKLN
CCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
SIKHETTPRVLTDSAEFNRVLGGGIVSGSLVLIGGDPGIGKSTLLLQICASLSQKKKVLY
HHHCCCCCCEEECCHHHHHHHCCCEEECCEEEEECCCCCCHHHHHHHHHHHHHCCCEEEE
ITGEESLSQTKLRAERLDEDSSELQVLAETDLEVIYQTVKEEQPDLLVVDSIQTIYHPEI
EECCCHHHHHHHHHHHCCCCCHHHEEHHHHHHHHHHHHHHCCCCCEEEEECHHHHHCCCC
SSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGDE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHEEEECCC
HHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVNNPSEMFLEERSTNVPGSTIVATMEGT
HHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCC
RPLLIEVQALVTPTTFNNPRRMATGIDHNRLSLLMAVLEKKENYLLQQQDAYIKVAGGVK
CCEEEEEEEEECCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHEECCCCEEEEECCEE
LTEPAVDLSVIVATASSFKDKAVDGLDCYIGEVGLTGEVRRVSRIEQRVQEAAKLGFKRV
ECCCCCCCEEEEEEHHHHHHHCCCCCHHEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEE
IIPKNNIGGWTYPEGIQVIGVTTVHEALSFALHS
EEECCCCCCCCCCCCEEEEEHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKKKVIFECMACGYQSPKWMGKCPNCGAWNQMEEIVEKAANPKHGVKTKELAGKVQKLN
CCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
SIKHETTPRVLTDSAEFNRVLGGGIVSGSLVLIGGDPGIGKSTLLLQICASLSQKKKVLY
HHHCCCCCCEEECCHHHHHHHCCCEEECCEEEEECCCCCCHHHHHHHHHHHHHCCCEEEE
ITGEESLSQTKLRAERLDEDSSELQVLAETDLEVIYQTVKEEQPDLLVVDSIQTIYHPEI
EECCCHHHHHHHHHHHCCCCCHHHEEHHHHHHHHHHHHHHCCCCCEEEEECHHHHHCCCC
SSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGDE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHEEEECCC
HHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVNNPSEMFLEERSTNVPGSTIVATMEGT
HHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCC
RPLLIEVQALVTPTTFNNPRRMATGIDHNRLSLLMAVLEKKENYLLQQQDAYIKVAGGVK
CCEEEEEEEEECCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHEECCCCEEEEECCEE
LTEPAVDLSVIVATASSFKDKAVDGLDCYIGEVGLTGEVRRVSRIEQRVQEAAKLGFKRV
ECCCCCCCEEEEEEHHHHHHHCCCCCHHEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEE
IIPKNNIGGWTYPEGIQVIGVTTVHEALSFALHS
EEECCCCCCCCCCCCEEEEEHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 8016066 [H]