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The map label for this gene is dfrA [H]

Identifier: 82703399

GI number: 82703399

Start: 2594014

End: 2595006

Strand: Reverse

Name: dfrA [H]

Synonym: Nmul_A2281

Alternate gene names: 82703399

Gene position: 2595006-2594014 (Counterclockwise)

Preceding gene: 82703400

Following gene: 82703389

Centisome position: 81.5

GC content: 55.09

Gene sequence:

>993_bases
ATGACTAAGTCTTTAATAACCGGAGCCAATGGATTTGTCGGCTCCGCAGTAACGCGCTGTTTACTGGAAGCGGGCCATGA
GGTGCGCTGTCTTGTCCGCCCGGGAAGCGACCGGCGAAATCTTGATAAGTTGCCCGTTGAAATTTCGGAGGGTGATTTGC
GTTCCGCTTCCTCGCTGAAACGGGCGGTCGCCGGGTGCGATAACTTGTTTCATGTGGCGGCAGACTACCGCCTGTGGGTA
CCCAATCCGGATACCATGTACGAAATCAATGTAAAGGGAACCCGGGCACTGGTTCTTGCAGCCGCCGAAGCTGGAATGAA
ACGCATGGTCTATACCAGCAGTGTGGCGACCCTGGGGACGGCTGAAAATGGCGTTCCTGCCGACGAAGATACGCCTTCCA
GCCTGGGATCGATGTGCGGACACTACAAGCGATCGAAATTCATGGCTGAAGAAATTGTTCAGCAGATGACACGGGAACAT
GACCTACCCATGGTCATAGTCAATCCTTCCACGCCGATAGGACCGCGTGATATCAAGCCAACCCCCACCGGCCGCCTCGT
GGTGGATACATTGCGCAATCGAATGCCGGCATACGTGAATACCGGCTTGAATATCGTGCACGCTGACGATATCGCTGAAG
GCCATCTGCTGGCATACAAGCATGGAAAACCCGGCGAACGTTACATCCTCGGGGGGGAAAACATGACCTTGCTGCAGATT
CTGCAGAAAATCGATGAGATAAGAGGCAGGCGGATCAGGCGGCTTGGCCTTCCCGTCAAGCTGATGGTGCCCGCTGCCTG
GTTGATGGAAAAAATGTCCACCGTTACCAAGGTTGAACCGCGCGCTACCGTGGACAGCGTCTCCATGGCAAAGAAAAAGA
TGTTCTACTCCAGCGACAAGGCGGTAAGAGAACTGGGTTATCGCTATCGCCCGGCTGCGGCTGCGCTCGAGGATGCAATG
AACTGGTTCCAGGCCAACGGTTACTGTGGCTAG

Upstream 100 bases:

>100_bases
GTGGTGGGCTCTGACAGGAGCAGCATTCATCGCTGAACGACAACGAAGCGAGTGATTGCTCGCAGGTTCCCCCCGAATCC
AGACGTCGAAAGGGTCAGAT

Downstream 100 bases:

>100_bases
GCCGGATATTTCATCGACTCACTGATAATGCCGAGGTGGACTCGCCGACGCGTTCTACTGTGCGCCTTTTCGGCTTCGGT
CCACCTGTTCCGGGGTAACG

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: DFR; Dihydrokaempferol 4-reductase [H]

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MTKSLITGANGFVGSAVTRCLLEAGHEVRCLVRPGSDRRNLDKLPVEISEGDLRSASSLKRAVAGCDNLFHVAADYRLWV
PNPDTMYEINVKGTRALVLAAAEAGMKRMVYTSSVATLGTAENGVPADEDTPSSLGSMCGHYKRSKFMAEEIVQQMTREH
DLPMVIVNPSTPIGPRDIKPTPTGRLVVDTLRNRMPAYVNTGLNIVHADDIAEGHLLAYKHGKPGERYILGGENMTLLQI
LQKIDEIRGRRIRRLGLPVKLMVPAAWLMEKMSTVTKVEPRATVDSVSMAKKKMFYSSDKAVRELGYRYRPAAAALEDAM
NWFQANGYCG

Sequences:

>Translated_330_residues
MTKSLITGANGFVGSAVTRCLLEAGHEVRCLVRPGSDRRNLDKLPVEISEGDLRSASSLKRAVAGCDNLFHVAADYRLWV
PNPDTMYEINVKGTRALVLAAAEAGMKRMVYTSSVATLGTAENGVPADEDTPSSLGSMCGHYKRSKFMAEEIVQQMTREH
DLPMVIVNPSTPIGPRDIKPTPTGRLVVDTLRNRMPAYVNTGLNIVHADDIAEGHLLAYKHGKPGERYILGGENMTLLQI
LQKIDEIRGRRIRRLGLPVKLMVPAAWLMEKMSTVTKVEPRATVDSVSMAKKKMFYSSDKAVRELGYRYRPAAAALEDAM
NWFQANGYCG
>Mature_329_residues
TKSLITGANGFVGSAVTRCLLEAGHEVRCLVRPGSDRRNLDKLPVEISEGDLRSASSLKRAVAGCDNLFHVAADYRLWVP
NPDTMYEINVKGTRALVLAAAEAGMKRMVYTSSVATLGTAENGVPADEDTPSSLGSMCGHYKRSKFMAEEIVQQMTREHD
LPMVIVNPSTPIGPRDIKPTPTGRLVVDTLRNRMPAYVNTGLNIVHADDIAEGHLLAYKHGKPGERYILGGENMTLLQIL
QKIDEIRGRRIRRLGLPVKLMVPAAWLMEKMSTVTKVEPRATVDSVSMAKKKMFYSSDKAVRELGYRYRPAAAALEDAMN
WFQANGYCG

Specific function: Galactose metabolism; third step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dihydroflavonol-4-reductase family [H]

Homologues:

Organism=Homo sapiens, GI116268111, Length=360, Percent_Identity=24.1666666666667, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI193211614, Length=340, Percent_Identity=23.2352941176471, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI8393516, Length=340, Percent_Identity=23.2352941176471, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI7657641, Length=268, Percent_Identity=27.9850746268657, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI239745448, Length=339, Percent_Identity=24.4837758112094, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI1786974, Length=304, Percent_Identity=25.3289473684211, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI87081792, Length=327, Percent_Identity=25.9938837920489, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71987463, Length=338, Percent_Identity=28.698224852071, Blast_Score=102, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6321437, Length=340, Percent_Identity=25, Blast_Score=73, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR017829
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.219 [H]

Molecular weight: Translated: 36322; Mature: 36190

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKSLITGANGFVGSAVTRCLLEAGHEVRCLVRPGSDRRNLDKLPVEISEGDLRSASSLK
CCCCEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEEECCCCCHHHHHHH
RAVAGCDNLFHVAADYRLWVPNPDTMYEINVKGTRALVLAAAEAGMKRMVYTSSVATLGT
HHHHCCCHHEEEEECCEEECCCCCCEEEEECCCCEEEEEEHHHHCHHHHHHHHHHHCCCC
AENGVPADEDTPSSLGSMCGHYKRSKFMAEEIVQQMTREHDLPMVIVNPSTPIGPRDIKP
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCC
TPTGRLVVDTLRNRMPAYVNTGLNIVHADDIAEGHLLAYKHGKPGERYILGGENMTLLQI
CCCCHHHHHHHHCCCCHHHCCCCEEEECCCCCCCCEEEEECCCCCCEEEECCCCHHHHHH
LQKIDEIRGRRIRRLGLPVKLMVPAAWLMEKMSTVTKVEPRATVDSVSMAKKKMFYSSDK
HHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCH
AVRELGYRYRPAAAALEDAMNWFQANGYCG
HHHHCCCCCCHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TKSLITGANGFVGSAVTRCLLEAGHEVRCLVRPGSDRRNLDKLPVEISEGDLRSASSLK
CCCEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEEECCCCCHHHHHHH
RAVAGCDNLFHVAADYRLWVPNPDTMYEINVKGTRALVLAAAEAGMKRMVYTSSVATLGT
HHHHCCCHHEEEEECCEEECCCCCCEEEEECCCCEEEEEEHHHHCHHHHHHHHHHHCCCC
AENGVPADEDTPSSLGSMCGHYKRSKFMAEEIVQQMTREHDLPMVIVNPSTPIGPRDIKP
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCC
TPTGRLVVDTLRNRMPAYVNTGLNIVHADDIAEGHLLAYKHGKPGERYILGGENMTLLQI
CCCCHHHHHHHHCCCCHHHCCCCEEEECCCCCCCCEEEEECCCCCCEEEECCCCHHHHHH
LQKIDEIRGRRIRRLGLPVKLMVPAAWLMEKMSTVTKVEPRATVDSVSMAKKKMFYSSDK
HHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCH
AVRELGYRYRPAAAALEDAMNWFQANGYCG
HHHHCCCCCCHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]