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The map label for this gene is rho [H]

Identifier: 82703205

GI number: 82703205

Start: 2377975

End: 2379234

Strand: Direct

Name: rho [H]

Synonym: Nmul_A2085

Alternate gene names: 82703205

Gene position: 2377975-2379234 (Clockwise)

Preceding gene: 82703204

Following gene: 82703206

Centisome position: 74.68

GC content: 53.97

Gene sequence:

>1260_bases
ATGCATCTATCCGACCTCAAAAACTTCCACGTCACCGAACTGGTGGAAATGGCAATTGCCAATGAAATTGACGGTGCTAA
CCGGTTACGGAAACAGGATCTGATTTTCGCATTATTGAAGAATCAGGCACGGAAAGGCGAAAGCATTTTCGGCGAAGGCA
CCTTGGAAGTGCTGCAGGATGGTTTTGGCTTCTTGCGTTCTCCCGACACCTCATATCTCGCCGGTCCGGATGACATTTAT
GTCTCACCCAGCCAGATACGGCGCTTCAACCTTCATACGGGGGATTCGATAGAGGGCGAAATAAGGACGCCCAAGGACGG
GGAGCGTTATTTCGCGCTGGTCAAGGTCGATAAGGTCAACGGAGAACCTCCTGAAAACTCCAAACACAAGATACTGTTTG
AAAATCTCACCCCCCTCTTTCCCACCGAGCGGCTGCAGCTCGAACGCGAGATCAAGGCCGAGGAAAACGTTACCAGTCGC
ATCATCGATCTGATCGCCCCCATCGGCAAAGGACAGCGGGGCCTCCTGGTAGCCAGTCCCAAATCAGGCAAGACGGTCAT
GCTTCAGCACATCGCCCACGCCATTGCCGCCAATTACCCGGATGTGATGCTGATGGTGCTGCTGATAGACGAGCGTCCCG
AAGAAGTGACCGAGATGATCCGGTCGGTAAGAGGTGAAGTGATTTCTTCCACTTTCGATGAGCCGGCTGTACGTCACGTG
CAAGTGGCGGATATGGTCATCGAAAAAGCCAAACGGTTGGTGGAACACAAGAAGGACGTGGTAATCCTGCTGGATTCGAT
CACCCGGCTCGCGCGCGCCTATAACACAGTGGTACCTGCTTCCGGCAAGGTGCTGACGGGGGGTGTGGATGCCAACGCGC
TGCAACGGCCGAAGCGGTTTTTTGGGGCTGCTCGCAATATCGAGGAGGGTGGCTCTCTCACCATTATTGCCACCGCGCTG
GTCGACACCGGCTCGCGCATGGACGATGTGATTTATGAGGAATTCAAGGGTACCGGCAACATGGAAATCCATCTTGACCG
GCGCATGGCAGAAAAACGCATTTATCCCGCCATCAACGTCAACCGTTCCGGCACCCGCCGGGAAGAACTCCTGATCAAGC
CCGATGTATTGCAGAAAATCTGGGTACTGCGCAAGCTGCTCTACCCGATGGATGACATGGAGGCAATGGAATTTCTGCTC
GACAAGATCAAGGCAACGAAGAACAACGCGGATTTCTTCGATTCAATGCGGCGGGTCTAA

Upstream 100 bases:

>100_bases
TTACGTTCGCCGCTTCGAATTCTCGTTCGGCTCCATCGGTTTTCCCAACGCTTCCTGACGCTTTCTTTACGTCCCTCTTC
TTCACCCACGGATTCCGTTC

Downstream 100 bases:

>100_bases
TATACTAACCTAAACATTGAATACCCCCCCTCTCCCGGAATGCCTTATTGCATCCCGTTTGCGAGTAGAGGATAATACGC
CGCTTTCCCCGAAATCAGGC

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MHLSDLKNFHVTELVEMAIANEIDGANRLRKQDLIFALLKNQARKGESIFGEGTLEVLQDGFGFLRSPDTSYLAGPDDIY
VSPSQIRRFNLHTGDSIEGEIRTPKDGERYFALVKVDKVNGEPPENSKHKILFENLTPLFPTERLQLEREIKAEENVTSR
IIDLIAPIGKGQRGLLVASPKSGKTVMLQHIAHAIAANYPDVMLMVLLIDERPEEVTEMIRSVRGEVISSTFDEPAVRHV
QVADMVIEKAKRLVEHKKDVVILLDSITRLARAYNTVVPASGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTIIATAL
VDTGSRMDDVIYEEFKGTGNMEIHLDRRMAEKRIYPAINVNRSGTRREELLIKPDVLQKIWVLRKLLYPMDDMEAMEFLL
DKIKATKNNADFFDSMRRV

Sequences:

>Translated_419_residues
MHLSDLKNFHVTELVEMAIANEIDGANRLRKQDLIFALLKNQARKGESIFGEGTLEVLQDGFGFLRSPDTSYLAGPDDIY
VSPSQIRRFNLHTGDSIEGEIRTPKDGERYFALVKVDKVNGEPPENSKHKILFENLTPLFPTERLQLEREIKAEENVTSR
IIDLIAPIGKGQRGLLVASPKSGKTVMLQHIAHAIAANYPDVMLMVLLIDERPEEVTEMIRSVRGEVISSTFDEPAVRHV
QVADMVIEKAKRLVEHKKDVVILLDSITRLARAYNTVVPASGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTIIATAL
VDTGSRMDDVIYEEFKGTGNMEIHLDRRMAEKRIYPAINVNRSGTRREELLIKPDVLQKIWVLRKLLYPMDDMEAMEFLL
DKIKATKNNADFFDSMRRV
>Mature_419_residues
MHLSDLKNFHVTELVEMAIANEIDGANRLRKQDLIFALLKNQARKGESIFGEGTLEVLQDGFGFLRSPDTSYLAGPDDIY
VSPSQIRRFNLHTGDSIEGEIRTPKDGERYFALVKVDKVNGEPPENSKHKILFENLTPLFPTERLQLEREIKAEENVTSR
IIDLIAPIGKGQRGLLVASPKSGKTVMLQHIAHAIAANYPDVMLMVLLIDERPEEVTEMIRSVRGEVISSTFDEPAVRHV
QVADMVIEKAKRLVEHKKDVVILLDSITRLARAYNTVVPASGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTIIATAL
VDTGSRMDDVIYEEFKGTGNMEIHLDRRMAEKRIYPAINVNRSGTRREELLIKPDVLQKIWVLRKLLYPMDDMEAMEFLL
DKIKATKNNADFFDSMRRV

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=418, Percent_Identity=70.3349282296651, Blast_Score=625, Evalue=1e-180,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 47216; Mature: 47216

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLSDLKNFHVTELVEMAIANEIDGANRLRKQDLIFALLKNQARKGESIFGEGTLEVLQD
CCCHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
GFGFLRSPDTSYLAGPDDIYVSPSQIRRFNLHTGDSIEGEIRTPKDGERYFALVKVDKVN
HHHHHCCCCCCCCCCCCCEEECHHHHHEEECCCCCCCCCCCCCCCCCCEEEEEEEEEECC
GEPPENSKHKILFENLTPLFPTERLQLEREIKAEENVTSRIIDLIAPIGKGQRGLLVASP
CCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECC
KSGKTVMLQHIAHAIAANYPDVMLMVLLIDERPEEVTEMIRSVRGEVISSTFDEPAVRHV
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHH
QVADMVIEKAKRLVEHKKDVVILLDSITRLARAYNTVVPASGKVLTGGVDANALQRPKRF
HHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHH
FGAARNIEEGGSLTIIATALVDTGSRMDDVIYEEFKGTGNMEIHLDRRMAEKRIYPAINV
HHHHCCCCCCCCEEEEEEEHHCCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHHCCCCEEC
NRSGTRREELLIKPDVLQKIWVLRKLLYPMDDMEAMEFLLDKIKATKNNADFFDSMRRV
CCCCCCHHHEEECHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCC
>Mature Secondary Structure
MHLSDLKNFHVTELVEMAIANEIDGANRLRKQDLIFALLKNQARKGESIFGEGTLEVLQD
CCCHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
GFGFLRSPDTSYLAGPDDIYVSPSQIRRFNLHTGDSIEGEIRTPKDGERYFALVKVDKVN
HHHHHCCCCCCCCCCCCCEEECHHHHHEEECCCCCCCCCCCCCCCCCCEEEEEEEEEECC
GEPPENSKHKILFENLTPLFPTERLQLEREIKAEENVTSRIIDLIAPIGKGQRGLLVASP
CCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECC
KSGKTVMLQHIAHAIAANYPDVMLMVLLIDERPEEVTEMIRSVRGEVISSTFDEPAVRHV
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHH
QVADMVIEKAKRLVEHKKDVVILLDSITRLARAYNTVVPASGKVLTGGVDANALQRPKRF
HHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHH
FGAARNIEEGGSLTIIATALVDTGSRMDDVIYEEFKGTGNMEIHLDRRMAEKRIYPAINV
HHHHCCCCCCCCEEEEEEEHHCCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHHCCCCEEC
NRSGTRREELLIKPDVLQKIWVLRKLLYPMDDMEAMEFLLDKIKATKNNADFFDSMRRV
CCCCCCHHHEEECHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782 [H]