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The map label for this gene is hemB [H]

Identifier: 82703199

GI number: 82703199

Start: 2371545

End: 2372549

Strand: Reverse

Name: hemB [H]

Synonym: Nmul_A2079

Alternate gene names: 82703199

Gene position: 2372549-2371545 (Counterclockwise)

Preceding gene: 82703200

Following gene: 229137829

Centisome position: 74.51

GC content: 56.22

Gene sequence:

>1005_bases
ATGCCAGCCATTAGTCAATTTCCCCAAAAAAGAATGCGGCGCATGCGCCGCAATGAATTTTCCCGCCGCCTGATGCGCGA
GAATCAGTTACATGCGGATGACCTGATCTATCCGGTATTCGTGCTGGATGGTGAAAAACGTGAGGAAAAAGTCCCGTCCA
TGCCCGGCGTTGTGCGGCAAAGCCTGGACGTTCTGCTATTCCGTGCCGAAAGATGCCTCGAACTGGGAATCCCGGCGCTG
GCTATTTTTCCGGTCATCGACGCCGGGCTGAAAAATGAAACCGCCTCCGAAGCGCTCAACCCTGCGGGGCTGGTACCGCG
TGTAGTAAGTGCGTTGAAAGCACGTTTTCCCGAACTGGGTATCATTACCGACATCGCCCTCGATCCTTACACGAGCCATG
GGCAGGATGGATTGATCGATCCGAGCGGTTATGTGATGAACGAGGAAACGGTGGCCGTTTTGACCCAGCAGGCCCTGGTG
CATGCGCAGGCAGGCGCTGACGTGGTGGCACCATCGGATATGATGGATGGCCGTATAGGCGCAATTCGCTCTGAATTGGA
GAACCGGGGCCTCATCCATACCCGTATCCTTGCTTATTCCGCAAAATACGCTTCCAGTTTTTATGGTCCGTTTCGCGATG
CGGTGGGATCGGCGGCCAATCTGGGTACCGGAAACAAATACACTTACCAGATGGATCCTGCCAACTCGGATGAAGCATTA
TGGGAAGTGGCCCTGGATCTGGAGGAGGGGGCCGATATGGTCATGATCAAGCCGGGGCTGCCTTATCTCGACATTGTGCG
ACGGATCAAGGAGCGCTTCGGAGCGCCCACCTTCGTTTATCAGGTGAGTGGCGAGTATGCGATGCTGAAAGCGGCCGGGC
AAAATGGCTGGCTGGATGAAAGGGCCTGTGTGCTTGAAGCGCTGCTCGCCTGCAAGCGCGCGGGCGCCGATGGCATTCTG
ACATACTATGCCACCGAAGTCGCTGAATGGCTGAGACAGCGCTGA

Upstream 100 bases:

>100_bases
AGACAAATAAACAACGTCTGTCAGTAAGATATAAAATCCAGCCACAAGTTCCCGGATTTTTGATTTTTTATCAGATTTTT
TCTTTTTTTGCAGGGCCATC

Downstream 100 bases:

>100_bases
GCCGAAATAAGACTGGTGGAGGACGACTGAAACAGGGCGAACAGGCAGGAAACGGGAATCGCCCCCTTTCTAGTTCGACA
GAAAATCGTTTATGATGTCC

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MPAISQFPQKRMRRMRRNEFSRRLMRENQLHADDLIYPVFVLDGEKREEKVPSMPGVVRQSLDVLLFRAERCLELGIPAL
AIFPVIDAGLKNETASEALNPAGLVPRVVSALKARFPELGIITDIALDPYTSHGQDGLIDPSGYVMNEETVAVLTQQALV
HAQAGADVVAPSDMMDGRIGAIRSELENRGLIHTRILAYSAKYASSFYGPFRDAVGSAANLGTGNKYTYQMDPANSDEAL
WEVALDLEEGADMVMIKPGLPYLDIVRRIKERFGAPTFVYQVSGEYAMLKAAGQNGWLDERACVLEALLACKRAGADGIL
TYYATEVAEWLRQR

Sequences:

>Translated_334_residues
MPAISQFPQKRMRRMRRNEFSRRLMRENQLHADDLIYPVFVLDGEKREEKVPSMPGVVRQSLDVLLFRAERCLELGIPAL
AIFPVIDAGLKNETASEALNPAGLVPRVVSALKARFPELGIITDIALDPYTSHGQDGLIDPSGYVMNEETVAVLTQQALV
HAQAGADVVAPSDMMDGRIGAIRSELENRGLIHTRILAYSAKYASSFYGPFRDAVGSAANLGTGNKYTYQMDPANSDEAL
WEVALDLEEGADMVMIKPGLPYLDIVRRIKERFGAPTFVYQVSGEYAMLKAAGQNGWLDERACVLEALLACKRAGADGIL
TYYATEVAEWLRQR
>Mature_333_residues
PAISQFPQKRMRRMRRNEFSRRLMRENQLHADDLIYPVFVLDGEKREEKVPSMPGVVRQSLDVLLFRAERCLELGIPALA
IFPVIDAGLKNETASEALNPAGLVPRVVSALKARFPELGIITDIALDPYTSHGQDGLIDPSGYVMNEETVAVLTQQALVH
AQAGADVVAPSDMMDGRIGAIRSELENRGLIHTRILAYSAKYASSFYGPFRDAVGSAANLGTGNKYTYQMDPANSDEALW
EVALDLEEGADMVMIKPGLPYLDIVRRIKERFGAPTFVYQVSGEYAMLKAAGQNGWLDERACVLEALLACKRAGADGILT
YYATEVAEWLRQR

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=308, Percent_Identity=43.1818181818182, Blast_Score=233, Evalue=2e-61,
Organism=Escherichia coli, GI87081728, Length=320, Percent_Identity=43.4375, Blast_Score=228, Evalue=3e-61,
Organism=Saccharomyces cerevisiae, GI6321398, Length=315, Percent_Identity=38.0952380952381, Blast_Score=195, Evalue=7e-51,
Organism=Drosophila melanogaster, GI21358291, Length=291, Percent_Identity=40.893470790378, Blast_Score=220, Evalue=1e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 36939; Mature: 36808

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAISQFPQKRMRRMRRNEFSRRLMRENQLHADDLIYPVFVLDGEKREEKVPSMPGVVRQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHCCCCHHHHHH
SLDVLLFRAERCLELGIPALAIFPVIDAGLKNETASEALNPAGLVPRVVSALKARFPELG
HHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHHCCCCC
IITDIALDPYTSHGQDGLIDPSGYVMNEETVAVLTQQALVHAQAGADVVAPSDMMDGRIG
EEEEEECCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHCCCCCCCCCHHHCCHHH
AIRSELENRGLIHTRILAYSAKYASSFYGPFRDAVGSAANLGTGNKYTYQMDPANSDEAL
HHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCEEEEEECCCCCHHHH
WEVALDLEEGADMVMIKPGLPYLDIVRRIKERFGAPTFVYQVSGEYAMLKAAGQNGWLDE
HHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCEEEEEECCCCCCCHH
RACVLEALLACKRAGADGILTYYATEVAEWLRQR
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PAISQFPQKRMRRMRRNEFSRRLMRENQLHADDLIYPVFVLDGEKREEKVPSMPGVVRQ
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHCCCCHHHHHH
SLDVLLFRAERCLELGIPALAIFPVIDAGLKNETASEALNPAGLVPRVVSALKARFPELG
HHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHHCCCCC
IITDIALDPYTSHGQDGLIDPSGYVMNEETVAVLTQQALVHAQAGADVVAPSDMMDGRIG
EEEEEECCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHCCCCCCCCCHHHCCHHH
AIRSELENRGLIHTRILAYSAKYASSFYGPFRDAVGSAANLGTGNKYTYQMDPANSDEAL
HHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCEEEEEECCCCCHHHH
WEVALDLEEGADMVMIKPGLPYLDIVRRIKERFGAPTFVYQVSGEYAMLKAAGQNGWLDE
HHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCEEEEEECCCCCCCHH
RACVLEALLACKRAGADGILTYYATEVAEWLRQR
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9529530; 10984043; 10356331; 12079382 [H]