Click here to switch to the map view.

The map label for this gene is pleD [H]

Identifier: 82702982

GI number: 82702982

Start: 2146194

End: 2147036

Strand: Direct

Name: pleD [H]

Synonym: Nmul_A1861

Alternate gene names: 82702982

Gene position: 2146194-2147036 (Clockwise)

Preceding gene: 82702981

Following gene: 82702988

Centisome position: 67.4

GC content: 48.75

Gene sequence:

>843_bases
ATGATCAATAAACCTGACACTCTGAACGCTAAAATTCTGATCGTGGATGATCAGGAAGCCAACGTCCGGCTGCTTGAACA
GATGCTGAGCGATGGGGGTTATAAAAACATTACCTCGACGATGGATCCGCGCGAAGTCAGCAATCTCTATCGGCAGAATC
GTTACGATCTCATTCTTCTTGATCTGTATATGCCTGGAATGGACGGTTTTCAGGTATTGGAAGCGCTAAAGAAAATTGAA
CGGGAAGGTTATCTTCCGGTACTCGTAATCACTGCGCAACCAGGTCATAAGTTGCGGGCACTGCAAGCAGGCGCAAAGGA
TTTCGTCAGCAAGCCCTTCGACCTCGTTGAAGTTCAAACCCGTATTCAAAATATGCTGGAAGTGCGCCTGCTGTATAAGC
AGTTGGAGGATTACAATAAAAGGCTGGAACAAACGGTAGCGGAGCGAACCGCAGAATTGCGCGAGAGTGAAGCCAGATTT
CGCCGTCTCACGGAACTGGCTTCCGACTGGTACTGGGAACAGGATGAAAAGGGAAATTTCACCAAAGTTTACGGTCCTGT
CCTGGATATGCTGGGGATTAAGGTCGACCACTTGCTGGGAGAATCCAAGGGATATAATGGAGCGCACTGGAATGAAGCGG
AGTATTCAGTTTTCAAGGCAAATATCGCGGCAAGACGGCCTTTTATCGATTTTATCTACAGACGGACCAAGCCGGATGGG
TCGACGCAATATCTAATGGTCAGTGGTGAGCCGATGTTCGATTCTTCCGGTCGCTTTACCGGCTACCGGGGTATTGGCAA
GGACGTCACCGATAGCATGCACGCAAATCAAGCTTCACAATGA

Upstream 100 bases:

>100_bases
AGAAGAATCAGTTTTTTCGTGCCCTATTCATCCATCTAGAGAGGTTACGAGCAGGGGAAAATGATGAAATCGGTTAACGG
GAACAAACAGGATAAAAATA

Downstream 100 bases:

>100_bases
CTGCTCAAACCTTATGAGTCTTTTCGGCGAGATGTAACCTTCCGTTGAAATTTCCCCCAACCCCAGAAACCGCTGCGCCT
TGTCATACAACCGTACCTGT

Product: putative PAS/PAC sensor protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MINKPDTLNAKILIVDDQEANVRLLEQMLSDGGYKNITSTMDPREVSNLYRQNRYDLILLDLYMPGMDGFQVLEALKKIE
REGYLPVLVITAQPGHKLRALQAGAKDFVSKPFDLVEVQTRIQNMLEVRLLYKQLEDYNKRLEQTVAERTAELRESEARF
RRLTELASDWYWEQDEKGNFTKVYGPVLDMLGIKVDHLLGESKGYNGAHWNEAEYSVFKANIAARRPFIDFIYRRTKPDG
STQYLMVSGEPMFDSSGRFTGYRGIGKDVTDSMHANQASQ

Sequences:

>Translated_280_residues
MINKPDTLNAKILIVDDQEANVRLLEQMLSDGGYKNITSTMDPREVSNLYRQNRYDLILLDLYMPGMDGFQVLEALKKIE
REGYLPVLVITAQPGHKLRALQAGAKDFVSKPFDLVEVQTRIQNMLEVRLLYKQLEDYNKRLEQTVAERTAELRESEARF
RRLTELASDWYWEQDEKGNFTKVYGPVLDMLGIKVDHLLGESKGYNGAHWNEAEYSVFKANIAARRPFIDFIYRRTKPDG
STQYLMVSGEPMFDSSGRFTGYRGIGKDVTDSMHANQASQ
>Mature_280_residues
MINKPDTLNAKILIVDDQEANVRLLEQMLSDGGYKNITSTMDPREVSNLYRQNRYDLILLDLYMPGMDGFQVLEALKKIE
REGYLPVLVITAQPGHKLRALQAGAKDFVSKPFDLVEVQTRIQNMLEVRLLYKQLEDYNKRLEQTVAERTAELRESEARF
RRLTELASDWYWEQDEKGNFTKVYGPVLDMLGIKVDHLLGESKGYNGAHWNEAEYSVFKANIAARRPFIDFIYRRTKPDG
STQYLMVSGEPMFDSSGRFTGYRGIGKDVTDSMHANQASQ

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87082012, Length=118, Percent_Identity=34.7457627118644, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788550, Length=137, Percent_Identity=29.9270072992701, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788191, Length=119, Percent_Identity=29.4117647058824, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1789809, Length=120, Percent_Identity=30.8333333333333, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789149, Length=121, Percent_Identity=29.7520661157025, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1790437, Length=120, Percent_Identity=31.6666666666667, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 32206; Mature: 32206

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS50113 PAC ; PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINKPDTLNAKILIVDDQEANVRLLEQMLSDGGYKNITSTMDPREVSNLYRQNRYDLILL
CCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCEEEE
DLYMPGMDGFQVLEALKKIEREGYLPVLVITAQPGHKLRALQAGAKDFVSKPFDLVEVQT
EEECCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHCHHHHHHCCHHHHHHHH
RIQNMLEVRLLYKQLEDYNKRLEQTVAERTAELRESEARFRRLTELASDWYWEQDEKGNF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
TKVYGPVLDMLGIKVDHLLGESKGYNGAHWNEAEYSVFKANIAARRPFIDFIYRRTKPDG
EHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCC
STQYLMVSGEPMFDSSGRFTGYRGIGKDVTDSMHANQASQ
CCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCC
>Mature Secondary Structure
MINKPDTLNAKILIVDDQEANVRLLEQMLSDGGYKNITSTMDPREVSNLYRQNRYDLILL
CCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCEEEE
DLYMPGMDGFQVLEALKKIEREGYLPVLVITAQPGHKLRALQAGAKDFVSKPFDLVEVQT
EEECCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHCHHHHHHCCHHHHHHHH
RIQNMLEVRLLYKQLEDYNKRLEQTVAERTAELRESEARFRRLTELASDWYWEQDEKGNF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
TKVYGPVLDMLGIKVDHLLGESKGYNGAHWNEAEYSVFKANIAARRPFIDFIYRRTKPDG
EHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCC
STQYLMVSGEPMFDSSGRFTGYRGIGKDVTDSMHANQASQ
CCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA