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The map label for this gene is dapE

Identifier: 82702973

GI number: 82702973

Start: 2135784

End: 2136920

Strand: Direct

Name: dapE

Synonym: Nmul_A1852

Alternate gene names: 82702973

Gene position: 2135784-2136920 (Clockwise)

Preceding gene: 82702972

Following gene: 82702975

Centisome position: 67.07

GC content: 54.27

Gene sequence:

>1137_bases
ATGCTCAATGACACACTGGCGCTCGCGCAAATGTTGATCGCCCGCCGCTCTCCGACACCTTTCGACAATGGTTGCCAGGA
AATCCTGATCGATCGCCTGGAAAAAATGGGCTTCGACATTGAAAGAATACGATGTGGAGAGGTGGATAATCTTTGGGCCA
GGCGCGGAACGGAAGCGCCGCTGATTTGCTTTGCCGGCCACACCGATGTCGTGCCCACCGGGCCCCTGGAAAAATGGGAA
AGCAGCCCGTTCGATCCCGTCACTCGAAATGGCAGGCTTTATGGGCGCGGGGCCGCAGACATGAAGGGATCGATAGCAGC
ATTCATCACCTCGATCGAGGCTTTCGTGGCGGAACATCCCGATCACAATGGCTCGATTGCGCTCCTGATCACGTCGGATG
AGGAAGGTATTGCGGTAGACGGTACTGTACGGGTGGTGGAAGTCCTCAAGGCACGCAATGAACTGATCGATTATTGCATC
GTCGGGGAACCGACCTCTGTCGACAAGCTTGGCGATATGATCAAGAACGGCCGACGCGGCTCCCTGTCCGGAACGTTAAC
CGTGAAAGGAATACAGGGCCATATTGCCTATCCTCACTTGGCAAAAAACCCAATCCACCTGGCGGCTCCCGCCATCGCCG
AACTGGCCAATACGGAGTGGGATGAAGGCAACGAATATTTCCCCCCTACCACCTGGCAGATTTCAAATATCAATGGAGGT
ACGGGTGCTACCAACGTGATTCCCGGTGAAGTAACGGTATTGTTCAACTTTCGTTTCTCCACTGCAAGCACGATAGAATC
GCTTAAAGCCCGAGTGCACGGAATTCTCGACCGGCATAACCTCGAATATGACTTGCAATGGGAAAATTCAGGCAAGCCTT
ATCTCACCCCAAGGGGCGACCTGGTCGATGCAGTGAATGCCGCCATTCACACCGTGACAGGAATCGAGCCTGAGCTTTCC
ACCTCGGGGGGAACCTCGGACGGCCGGTTCATCGCCGACATCTGCCCGCAAGTGGTGGAATTGGGACCACGGAATGCAAC
CATCCACAAGATCAACGAATACGTTGAAGTTTCGGATCTGGATCAGTTGCCCCGGATTTACCAGCTCACGATGGAAAGCC
TGTTGCTGAGAAAATAA

Upstream 100 bases:

>100_bases
CCGAGCTAAAACCGGTATCAATGAGCTGTTAAGGGGAATTTAACATCCGGAATTTAACACCGACTTTCATCCACAACAGG
CTTCTTTTCAGGAATTGCTC

Downstream 100 bases:

>100_bases
GAATAAAAATGGAGATGGACCATTTCTACGGGCGGGACTGACCCGGACGTACACGCACATCACCCACTGCCCGCTCTCGC
AACTCCCGCAGCACCTCCAG

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MLNDTLALAQMLIARRSPTPFDNGCQEILIDRLEKMGFDIERIRCGEVDNLWARRGTEAPLICFAGHTDVVPTGPLEKWE
SSPFDPVTRNGRLYGRGAADMKGSIAAFITSIEAFVAEHPDHNGSIALLITSDEEGIAVDGTVRVVEVLKARNELIDYCI
VGEPTSVDKLGDMIKNGRRGSLSGTLTVKGIQGHIAYPHLAKNPIHLAAPAIAELANTEWDEGNEYFPPTTWQISNINGG
TGATNVIPGEVTVLFNFRFSTASTIESLKARVHGILDRHNLEYDLQWENSGKPYLTPRGDLVDAVNAAIHTVTGIEPELS
TSGGTSDGRFIADICPQVVELGPRNATIHKINEYVEVSDLDQLPRIYQLTMESLLLRK

Sequences:

>Translated_378_residues
MLNDTLALAQMLIARRSPTPFDNGCQEILIDRLEKMGFDIERIRCGEVDNLWARRGTEAPLICFAGHTDVVPTGPLEKWE
SSPFDPVTRNGRLYGRGAADMKGSIAAFITSIEAFVAEHPDHNGSIALLITSDEEGIAVDGTVRVVEVLKARNELIDYCI
VGEPTSVDKLGDMIKNGRRGSLSGTLTVKGIQGHIAYPHLAKNPIHLAAPAIAELANTEWDEGNEYFPPTTWQISNINGG
TGATNVIPGEVTVLFNFRFSTASTIESLKARVHGILDRHNLEYDLQWENSGKPYLTPRGDLVDAVNAAIHTVTGIEPELS
TSGGTSDGRFIADICPQVVELGPRNATIHKINEYVEVSDLDQLPRIYQLTMESLLLRK
>Mature_378_residues
MLNDTLALAQMLIARRSPTPFDNGCQEILIDRLEKMGFDIERIRCGEVDNLWARRGTEAPLICFAGHTDVVPTGPLEKWE
SSPFDPVTRNGRLYGRGAADMKGSIAAFITSIEAFVAEHPDHNGSIALLITSDEEGIAVDGTVRVVEVLKARNELIDYCI
VGEPTSVDKLGDMIKNGRRGSLSGTLTVKGIQGHIAYPHLAKNPIHLAAPAIAELANTEWDEGNEYFPPTTWQISNINGG
TGATNVIPGEVTVLFNFRFSTASTIESLKARVHGILDRHNLEYDLQWENSGKPYLTPRGDLVDAVNAAIHTVTGIEPELS
TSGGTSDGRFIADICPQVVELGPRNATIHKINEYVEVSDLDQLPRIYQLTMESLLLRK

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily

Homologues:

Organism=Homo sapiens, GI21071039, Length=132, Percent_Identity=30.3030303030303, Blast_Score=66, Evalue=6e-11,
Organism=Escherichia coli, GI1788816, Length=371, Percent_Identity=57.1428571428571, Blast_Score=446, Evalue=1e-126,
Organism=Escherichia coli, GI1790395, Length=349, Percent_Identity=25.5014326647564, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPE_NITMU (Q2Y7X4)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_412539.1
- ProteinModelPortal:   Q2Y7X4
- SMR:   Q2Y7X4
- STRING:   Q2Y7X4
- MEROPS:   M20.010
- GeneID:   3786594
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A1852
- eggNOG:   COG0624
- HOGENOM:   HBG728841
- OMA:   PMVFEDT
- PhylomeDB:   Q2Y7X4
- ProtClustDB:   PRK13009
- BioCyc:   NMUL323848:NMUL_A1852-MONOMER
- HAMAP:   MF_01690
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650
- TIGRFAMs:   TIGR01246

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.5.1.18

Molecular weight: Translated: 41351; Mature: 41351

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 69-69 ACT_SITE 134-134

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNDTLALAQMLIARRSPTPFDNGCQEILIDRLEKMGFDIERIRCGEVDNLWARRGTEAP
CCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHCCCCCC
LICFAGHTDVVPTGPLEKWESSPFDPVTRNGRLYGRGAADMKGSIAAFITSIEAFVAEHP
EEEECCCCCCCCCCCHHHHCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCC
DHNGSIALLITSDEEGIAVDGTVRVVEVLKARNELIDYCIVGEPTSVDKLGDMIKNGRRG
CCCCCEEEEEECCCCCEEECCHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHCCCCC
SLSGTLTVKGIQGHIAYPHLAKNPIHLAAPAIAELANTEWDEGNEYFPPTTWQISNINGG
CCCEEEEEEECCCCEECCCCCCCCCEEHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCC
TGATNVIPGEVTVLFNFRFSTASTIESLKARVHGILDRHNLEYDLQWENSGKPYLTPRGD
CCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEECCCCH
LVDAVNAAIHTVTGIEPELSTSGGTSDGRFIADICPQVVELGPRNATIHKINEYVEVSDL
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCH
DQLPRIYQLTMESLLLRK
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLNDTLALAQMLIARRSPTPFDNGCQEILIDRLEKMGFDIERIRCGEVDNLWARRGTEAP
CCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHCCCCCC
LICFAGHTDVVPTGPLEKWESSPFDPVTRNGRLYGRGAADMKGSIAAFITSIEAFVAEHP
EEEECCCCCCCCCCCHHHHCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCC
DHNGSIALLITSDEEGIAVDGTVRVVEVLKARNELIDYCIVGEPTSVDKLGDMIKNGRRG
CCCCCEEEEEECCCCCEEECCHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHCCCCC
SLSGTLTVKGIQGHIAYPHLAKNPIHLAAPAIAELANTEWDEGNEYFPPTTWQISNINGG
CCCEEEEEEECCCCEECCCCCCCCCEEHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCC
TGATNVIPGEVTVLFNFRFSTASTIESLKARVHGILDRHNLEYDLQWENSGKPYLTPRGD
CCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEECCCCH
LVDAVNAAIHTVTGIEPELSTSGGTSDGRFIADICPQVVELGPRNATIHKINEYVEVSDL
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCH
DQLPRIYQLTMESLLLRK
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA