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The map label for this gene is pchF [H]

Identifier: 82702604

GI number: 82702604

Start: 1683961

End: 1685505

Strand: Reverse

Name: pchF [H]

Synonym: Nmul_A1475

Alternate gene names: 82702604

Gene position: 1685505-1683961 (Counterclockwise)

Preceding gene: 82702605

Following gene: 82702602

Centisome position: 52.93

GC content: 53.98

Gene sequence:

>1545_bases
ATGTCAAATAAATATATCGCATTGCCGAAAGGAATATCCGAAAAGACTTTCGATGCTGCCATAGCGGATTTTCGCGGCGT
ACTCGGCGACGCAGCCGTGCTTACCAGCGCTCAGCAGCTTGCCCCTTACATCAAAACGATGATGCCCGTGCCTGATTCGG
ACCACGCTCCATCTGCCGCGTTAATGGCGACGACGGTGGAGGAGATTCAGAAAATTGTCGGGATATGTAACCGATACAAG
GTTCCCATCTGGACCATTTCCACCGGAAAAAATCTGGGCTACGGCTCGGCGGCACCCGCTGAACGCGGACAGGTTGTCCT
CGACCTCAAGCGAATGAACCGCATACTGGAAGTCGATAAAGATCTGGCCTATGCCCTGGTTGAGCCGGGAGTGACTTATA
AGCAGCTCTATGACTATATCGCCGAGCACAAGCTGGGGCTGTGGCTATCCATGCCCGCTCCTTCCGCAATTGCAGGACCG
GTTGGCAATACGCTTGATCGTGGAGTCGGCTATACCCCTTACGGAGAACATTTCCTGTTTGCCTGCGGTATGGAGGTGGT
CCTGGCAAATGGAGAAGTGCTCCGCACCGGAATGGGGTCGATGCCGAATTCCAATACCTGGCAGGTTTTCAAATGGGGAT
ACGGACCCTATCTGGACGGGATTTTCACACAATCCAATTATGGCATCGTCACCAAATTCGGCTTCTGGCTTATGCCCGCT
CCACCGGCTTTCAAGCCTTTTGTCATTCAGTACGAGAATGAGGAAGATATCGTGGAGATCGTGGAAATCATTCGTCCGCT
GCGCATCAACGGGGTGATCCCAAATGCGGTTGTCATTGCCCACGCGTTATATGAAGCCCCTGTCAAGGCTCGCCGCAGTG
ACTATGTGACCGGCCCGGGGTCTATTCCGGATGTGGCAGTGCGCAAGCTCGCGAAGGATCATCACTTGGGGATCTGGAAT
GTGTATGCAGCATTGTATGGTACGCAGGAGCAGATCGACCTTAACTGGAAAATCGTGACGCAAGCATTCGGTAGATCCGG
CAAAGCGAAGATTCTGACCGAGCGGGAGGCGGGCGATGATCCTGCCTTTAAATATCGCGCATCTCTCATGCGCGGTGACA
TGACGCTTCAGGAATTCTCGTTGTACAACTGGCGCGGGGGCGGGGGCTCCATGTGGTTTGCGCCGGTGTCTCAAGCAAGG
GGAAGCGAGACGCTGAAACAGATGGCGTTAACCAAGCAGATCCTTGCCAAGTATGGCCTGGATTATTCCGGGGAATTCAT
CGTCGGAATGCGTGACATGCATCATATTGTGGACGTGCTCTATGACAAGACCGACCCGGGTCAGGTCAAGGCGGCATACC
AGTGTTTCGATGAACTGCTGACAGTCTTTTCTGCGCAGGGGTATGGGACCTACCGGGTCAATACGGCGTTTATGGATAAG
GTGGCGGAAACGTATGGTCCGGTACAACGTCAAGTCCACAAGACGCTAAAGAAGGCGCTGGACCCGAACGGTATCCTGGC
CCCGGGAAAATCCGGAATTCGCTGA

Upstream 100 bases:

>100_bases
CATCTACCGCCCGGTACACGTCCTCGCCGGAATATACGCATCGAACGATTCGCCTCATTTGTGATAGAGACCTAACCCGG
ATAGGAGCAAGGGACTCAAA

Downstream 100 bases:

>100_bases
CCCTTTACTGGCCGATAAATTCAATCGGCCAGCAGGATGTATATTTTCAGTGAAGGTGCCTCCACTGAGGGGAGGGGAGG
TGCTTCTTGATCAGGATAGT

Product: FAD linked oxidase-like protein

Products: NA

Alternate protein names: P-cresol methylhydroxylase; PCMH [H]

Number of amino acids: Translated: 514; Mature: 513

Protein sequence:

>514_residues
MSNKYIALPKGISEKTFDAAIADFRGVLGDAAVLTSAQQLAPYIKTMMPVPDSDHAPSAALMATTVEEIQKIVGICNRYK
VPIWTISTGKNLGYGSAAPAERGQVVLDLKRMNRILEVDKDLAYALVEPGVTYKQLYDYIAEHKLGLWLSMPAPSAIAGP
VGNTLDRGVGYTPYGEHFLFACGMEVVLANGEVLRTGMGSMPNSNTWQVFKWGYGPYLDGIFTQSNYGIVTKFGFWLMPA
PPAFKPFVIQYENEEDIVEIVEIIRPLRINGVIPNAVVIAHALYEAPVKARRSDYVTGPGSIPDVAVRKLAKDHHLGIWN
VYAALYGTQEQIDLNWKIVTQAFGRSGKAKILTEREAGDDPAFKYRASLMRGDMTLQEFSLYNWRGGGGSMWFAPVSQAR
GSETLKQMALTKQILAKYGLDYSGEFIVGMRDMHHIVDVLYDKTDPGQVKAAYQCFDELLTVFSAQGYGTYRVNTAFMDK
VAETYGPVQRQVHKTLKKALDPNGILAPGKSGIR

Sequences:

>Translated_514_residues
MSNKYIALPKGISEKTFDAAIADFRGVLGDAAVLTSAQQLAPYIKTMMPVPDSDHAPSAALMATTVEEIQKIVGICNRYK
VPIWTISTGKNLGYGSAAPAERGQVVLDLKRMNRILEVDKDLAYALVEPGVTYKQLYDYIAEHKLGLWLSMPAPSAIAGP
VGNTLDRGVGYTPYGEHFLFACGMEVVLANGEVLRTGMGSMPNSNTWQVFKWGYGPYLDGIFTQSNYGIVTKFGFWLMPA
PPAFKPFVIQYENEEDIVEIVEIIRPLRINGVIPNAVVIAHALYEAPVKARRSDYVTGPGSIPDVAVRKLAKDHHLGIWN
VYAALYGTQEQIDLNWKIVTQAFGRSGKAKILTEREAGDDPAFKYRASLMRGDMTLQEFSLYNWRGGGGSMWFAPVSQAR
GSETLKQMALTKQILAKYGLDYSGEFIVGMRDMHHIVDVLYDKTDPGQVKAAYQCFDELLTVFSAQGYGTYRVNTAFMDK
VAETYGPVQRQVHKTLKKALDPNGILAPGKSGIR
>Mature_513_residues
SNKYIALPKGISEKTFDAAIADFRGVLGDAAVLTSAQQLAPYIKTMMPVPDSDHAPSAALMATTVEEIQKIVGICNRYKV
PIWTISTGKNLGYGSAAPAERGQVVLDLKRMNRILEVDKDLAYALVEPGVTYKQLYDYIAEHKLGLWLSMPAPSAIAGPV
GNTLDRGVGYTPYGEHFLFACGMEVVLANGEVLRTGMGSMPNSNTWQVFKWGYGPYLDGIFTQSNYGIVTKFGFWLMPAP
PAFKPFVIQYENEEDIVEIVEIIRPLRINGVIPNAVVIAHALYEAPVKARRSDYVTGPGSIPDVAVRKLAKDHHLGIWNV
YAALYGTQEQIDLNWKIVTQAFGRSGKAKILTEREAGDDPAFKYRASLMRGDMTLQEFSLYNWRGGGGSMWFAPVSQARG
SETLKQMALTKQILAKYGLDYSGEFIVGMRDMHHIVDVLYDKTDPGQVKAAYQCFDELLTVFSAQGYGTYRVNTAFMDKV
AETYGPVQRQVHKTLKKALDPNGILAPGKSGIR

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=459, Percent_Identity=24.1830065359477, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI37595754, Length=476, Percent_Identity=23.9495798319328, Blast_Score=83, Evalue=6e-16,
Organism=Escherichia coli, GI1789351, Length=226, Percent_Identity=26.1061946902655, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR006094
- InterPro:   IPR016170
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: =1.17.99.1 [H]

Molecular weight: Translated: 56709; Mature: 56578

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNKYIALPKGISEKTFDAAIADFRGVLGDAAVLTSAQQLAPYIKTMMPVPDSDHAPSAA
CCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
LMATTVEEIQKIVGICNRYKVPIWTISTGKNLGYGSAAPAERGQVVLDLKRMNRILEVDK
HHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHH
DLAYALVEPGVTYKQLYDYIAEHKLGLWLSMPAPSAIAGPVGNTLDRGVGYTPYGEHFLF
HHHHHHHCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHCCCCCCCCCCHHHH
ACGMEVVLANGEVLRTGMGSMPNSNTWQVFKWGYGPYLDGIFTQSNYGIVTKFGFWLMPA
HCCEEEEEECCCEEECCCCCCCCCCCEEEEEECCCCHHCCEEECCCCCEEEECCEEEECC
PPAFKPFVIQYENEEDIVEIVEIIRPLRINGVIPNAVVIAHALYEAPVKARRSDYVTGPG
CCCCCCEEEEECCCHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHCCHHHHCCCCCCCCC
SIPDVAVRKLAKDHHLGIWNVYAALYGTQEQIDLNWKIVTQAFGRSGKAKILTEREAGDD
CCHHHHHHHHHHHCCCCHHHHHHHHHCCCHHEECCEEEEEHHHCCCCCEEEEECCCCCCC
PAFKYRASLMRGDMTLQEFSLYNWRGGGGSMWFAPVSQARGSETLKQMALTKQILAKYGL
HHHHHHHHHHHCCCCHHHHHEEEEECCCCCEEECCHHHCCCHHHHHHHHHHHHHHHHHCC
DYSGEFIVGMRDMHHIVDVLYDKTDPGQVKAAYQCFDELLTVFSAQGYGTYRVNTAFMDK
CCCCCEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHH
VAETYGPVQRQVHKTLKKALDPNGILAPGKSGIR
HHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCC
>Mature Secondary Structure 
SNKYIALPKGISEKTFDAAIADFRGVLGDAAVLTSAQQLAPYIKTMMPVPDSDHAPSAA
CCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
LMATTVEEIQKIVGICNRYKVPIWTISTGKNLGYGSAAPAERGQVVLDLKRMNRILEVDK
HHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHH
DLAYALVEPGVTYKQLYDYIAEHKLGLWLSMPAPSAIAGPVGNTLDRGVGYTPYGEHFLF
HHHHHHHCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHCCCCCCCCCCHHHH
ACGMEVVLANGEVLRTGMGSMPNSNTWQVFKWGYGPYLDGIFTQSNYGIVTKFGFWLMPA
HCCEEEEEECCCEEECCCCCCCCCCCEEEEEECCCCHHCCEEECCCCCEEEECCEEEECC
PPAFKPFVIQYENEEDIVEIVEIIRPLRINGVIPNAVVIAHALYEAPVKARRSDYVTGPG
CCCCCCEEEEECCCHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHCCHHHHCCCCCCCCC
SIPDVAVRKLAKDHHLGIWNVYAALYGTQEQIDLNWKIVTQAFGRSGKAKILTEREAGDD
CCHHHHHHHHHHHCCCCHHHHHHHHHCCCHHEECCEEEEEHHHCCCCCEEEEECCCCCCC
PAFKYRASLMRGDMTLQEFSLYNWRGGGGSMWFAPVSQARGSETLKQMALTKQILAKYGL
HHHHHHHHHHHCCCCHHHHHEEEEECCCCCEEECCHHHCCCHHHHHHHHHHHHHHHHHCC
DYSGEFIVGMRDMHHIVDVLYDKTDPGQVKAAYQCFDELLTVFSAQGYGTYRVNTAFMDK
CCCCCEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHH
VAETYGPVQRQVHKTLKKALDPNGILAPGKSGIR
HHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7929007; 10565539; 3790500; 1846290; 10623531 [H]