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The map label for this gene is frvA

Identifier: 82546248

GI number: 82546248

Start: 3945417

End: 3945863

Strand: Reverse

Name: frvA

Synonym: SBO_3916

Alternate gene names: 82546248

Gene position: 3945863-3945417 (Counterclockwise)

Preceding gene: 82546249

Following gene: 82546247

Centisome position: 87.3

GC content: 49.89

Gene sequence:

>447_bases
ATGGCAGCTCTTACTGCAAGCTGTATTGACCTGAATATTCAGGGCAATGGCGCTTATTCCGTTCTGAAGCAGTTGGCGAC
AATAGCGTTACAAAACGGTTTTATCACCGACTCACACCAGTTCCTGCAAACCCTGCTGCTGCGCGAAAAAATGCACTCTA
CTGGATTTGGTTCCGGTGTCGCCGTGCCGCACGGTAAAAGCGCCTGCGTTAAACAACCGTTCGTATTATTCGCCCGCAAA
GCGCAGGCTATTGACTGGAAAGCCAGCGATGGCGAAGACGTCAATTGCTGGATCTGCCTCGGCGTGCCGCAAAGCGGCGA
AGAGGATCAGGTCAAAATCATCGGCACACTGTGTCGCAAAATTATTCACAAGGAATTTATTCATCAACTGCAACAGGGCG
ATACCGACCAAGTGCTTGCCTTGTTAAATCAAACCCTCAGCTCATAA

Upstream 100 bases:

>100_bases
CTCTGTCTCAAATTGTCATTGTTTACCGACATATCGCCTCTCATCACTTAATCGCTCCACCATTACAATTGATTAAAATA
TATTTTATGGAGTCCGATTA

Downstream 100 bases:

>100_bases
GGAAGTGGCGATGGAGTCATCCTTACGTATTGTCGCGATCACCAACTGCCCCGCCGGGATCGCTCACACCTACATGGTGG
CGGAAGCCCTGGAACAGAAA

Product: fructose-like phosphotransferase system subunit EIIA

Products: protein histidine; sugar phosphate

Alternate protein names: PTS system fructose-like EIIA component

Number of amino acids: Translated: 148; Mature: 147

Protein sequence:

>148_residues
MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQTLLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARK
AQAIDWKASDGEDVNCWICLGVPQSGEEDQVKIIGTLCRKIIHKEFIHQLQQGDTDQVLALLNQTLSS

Sequences:

>Translated_148_residues
MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQTLLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARK
AQAIDWKASDGEDVNCWICLGVPQSGEEDQVKIIGTLCRKIIHKEFIHQLQQGDTDQVLALLNQTLSS
>Mature_147_residues
AALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQTLLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKA
QAIDWKASDGEDVNCWICLGVPQSGEEDQVKIIGTLCRKIIHKEFIHQLQQGDTDQVLALLNQTLSS

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain

Homologues:

Organism=Escherichia coli, GI2367327, Length=148, Percent_Identity=100, Blast_Score=304, Evalue=1e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTFA_ECOLI (P32155)

Other databases:

- EMBL:   X60472
- EMBL:   L19201
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   D48649
- RefSeq:   AP_003908.1
- RefSeq:   NP_418336.1
- ProteinModelPortal:   P32155
- SMR:   P32155
- IntAct:   P32155
- STRING:   P32155
- EnsemblBacteria:   EBESCT00000001529
- EnsemblBacteria:   EBESCT00000014696
- GeneID:   948391
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW3871
- KEGG:   eco:b3900
- EchoBASE:   EB1810
- EcoGene:   EG11864
- eggNOG:   COG1762
- GeneTree:   EBGT00050000009030
- HOGENOM:   HBG697201
- OMA:   HAKSKYV
- ProtClustDB:   PRK09913
- BioCyc:   EcoCyc:FRVA-MONOMER
- Genevestigator:   P32155
- GO:   GO:0005737
- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR004715
- Gene3D:   G3DSA:3.40.930.10
- TIGRFAMs:   TIGR00848

Pfam domain/function: PF00359 PTS_EIIA_2; SSF55804 PTrfase/Anion_transptr

EC number: 2.7.1.69

Molecular weight: Translated: 16094; Mature: 15962

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: PS51094 PTS_EIIA_TYPE_2; PS00372 PTS_EIIA_TYPE_2_HIS

Important sites: ACT_SITE 64-64

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQTLLLREKMHSTGFGSGV
CCCEEEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
AVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGVPQSGEEDQVKIIGTLCRK
CCCCCCCHHHHCHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHH
IIHKEFIHQLQQGDTDQVLALLNQTLSS
HHHHHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
AALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQTLLLREKMHSTGFGSGV
CCEEEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
AVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGVPQSGEEDQVKIIGTLCRK
CCCCCCCHHHHCHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHH
IIHKEFIHQLQQGDTDQVLALLNQTLSS
HHHHHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8396120; 8346018; 9278503; 8019415