Click here to switch to the map view.

The map label for this gene is yieO [H]

Identifier: 82546118

GI number: 82546118

Start: 3787391

End: 3788818

Strand: Reverse

Name: yieO [H]

Synonym: SBO_3768

Alternate gene names: 82546118

Gene position: 3788818-3787391 (Counterclockwise)

Preceding gene: 82546119

Following gene: 82546112

Centisome position: 83.83

GC content: 49.3

Gene sequence:

>1428_bases
ATGAGCGATAAAAAGAAGCGTAGTATGGCGGGTTTGCCGTGGATCGCGGCGATGGCCTTCTTCATGCAGGCACTTGATGC
CACTATTCTGAATACCGCCTTACCCGCAATCGCTCATAGCCTTAATCGTTCTCCTCTCGCGATGCAATCAGCCATCATCA
GTTATACGCTGACGGTGGCGATGTTTATTCCGGTAAGCGGATGGCTAGCCGATCGCTTCGGTACGCGTCGCATTTTTACC
CTTGCCGTGAGTCTGTTCACGTTGGGTTCTCTGGCCTGCGCACTTTCTAATTCGCTACCACAGCTGGTTGTCTTCCGGGT
TATTCAGGGGATAGGCGGCGCAATGATGATGCCTGTTGCTCGGCTGGCCTTACTGCGTGCTTATCCTCGTAATGAACTTC
TTCCTGTATTGAATTTTGTCGCCATGCCGGGTCTGGTGGGGCCAATTTTAGGCCCCGTTCTTGGCGGCGTGCTGGTCACC
TGGGCAACCTGGCACTGGATATTTTTAATCAATATCCCCATAGGTATTGCGGGCCTTCTTTACGCGCGCAAACATATGCC
CAATTTCACCACCGCACGACGCAGATTCGATATCACTGGCTTTTTGCTGTTTGGCCTCAGCCTTGTTCTCTTCTCAAGCG
GAATAGAGCTATTCGGGGAAAAGATTGTCGCCAGCTGGATTGCCTTGACGGTAATTGTCACCAGCATCGGGTTACTGCTT
CTCTATATTCTCCATGCGCGACACACGCCAAACCCATTAATTTCATTAGATTTATTTAAAACCCGCACTTTCTCGATCGG
TATCGTAGGCAATATTGCAACCCGTCTGGGGACCGGTAGTGTACCGTTCCTTATGCCATTGATGTTACAGGTAGGATTTG
GTTATCAGGCGTTTATTGCCGGCTGTATGATGGCACCGACAGCGTTAGGTTCCATTATTGCAAAATCGATGGTTACCCAA
GTCTTACGTCGTCTGGGCTATCGCCATACGTTAGTGGGGATCACGGTGATTATTGGGCTAATGATCGCTCAGTTCTCTTT
GCAATCACCGGCAATGGCGATATGGATGCTGATCTTGCCGTTGTTTATATTAGGGATGGCTATGTCGACGCAATTTACCG
CGATGAATACCATCACACTTGCCGATCTGACCGATGACAACGCCAGCAGCGGTAACAGTGTTCTGGCGGTCACGCAGCAA
CTGTCGATTAGTTTAGGCGTTGCTGTAAGTGCGGCCGTCCTTCGCGTTTATGAAGGGATGGAAGGCACAACGACTGTCGA
ACAATTCCACTATACGTTTATCACGATGGGCATTATTACTGTTGCTTCAGCAGCAATGTTCATGCTTCTGAAAACAACCG
ATGGTAATAATTTGATCAAAAGACAGCGTAAATCTAAGCCGAACCGCGTTCCATCAGAATCGGAGTAA

Upstream 100 bases:

>100_bases
GTCGATGCGATTATCCAAAGCGATGGCGACGCGGCATTTAAAACTTGTCAGGCGCTGCTACGCTCACCTGATAAGTGATA
ACCCGATAACAGGACATTGA

Downstream 100 bases:

>100_bases
GTTGTAATCGTTGTTGCTGAAGGGTCGGCTGGGTTATCCGATGGATGAGTACATCAATCGCCAGCTCCCCCAGTTCATCT
TTCGGTTGGTGGATAGTGGT

Product: transport protein

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: High-copy suppressor of rspA [H]

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRSPLAMQSAIISYTLTVAMFIPVSGWLADRFGTRRIFT
LAVSLFTLGSLACALSNSLPQLVVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELFGEKIVASWIALTVIVTSIGLLL
LYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRLGTGSVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQ
VLRRLGYRHTLVGITVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGNSVLAVTQQ
LSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLLKTTDGNNLIKRQRKSKPNRVPSESE

Sequences:

>Translated_475_residues
MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRSPLAMQSAIISYTLTVAMFIPVSGWLADRFGTRRIFT
LAVSLFTLGSLACALSNSLPQLVVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELFGEKIVASWIALTVIVTSIGLLL
LYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRLGTGSVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQ
VLRRLGYRHTLVGITVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGNSVLAVTQQ
LSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLLKTTDGNNLIKRQRKSKPNRVPSESE
>Mature_474_residues
SDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRSPLAMQSAIISYTLTVAMFIPVSGWLADRFGTRRIFTL
AVSLFTLGSLACALSNSLPQLVVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVTW
ATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELFGEKIVASWIALTVIVTSIGLLLL
YILHARHTPNPLISLDLFKTRTFSIGIVGNIATRLGTGSVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQV
LRRLGYRHTLVGITVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGNSVLAVTQQL
SISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLLKTTDGNNLIKRQRKSKPNRVPSESE

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=475, Percent_Identity=99.3684210526316, Blast_Score=941, Evalue=0.0,
Organism=Escherichia coli, GI1788392, Length=459, Percent_Identity=45.5337690631808, Blast_Score=363, Evalue=1e-101,
Organism=Escherichia coli, GI1789042, Length=426, Percent_Identity=32.1596244131455, Blast_Score=168, Evalue=7e-43,
Organism=Escherichia coli, GI1788710, Length=437, Percent_Identity=32.2654462242563, Blast_Score=163, Evalue=3e-41,
Organism=Escherichia coli, GI87081983, Length=414, Percent_Identity=28.5024154589372, Blast_Score=125, Evalue=7e-30,
Organism=Escherichia coli, GI1788509, Length=136, Percent_Identity=29.4117647058824, Blast_Score=63, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6322958, Length=478, Percent_Identity=25.9414225941423, Blast_Score=87, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6325455, Length=127, Percent_Identity=33.0708661417323, Blast_Score=71, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6321663, Length=147, Percent_Identity=31.2925170068027, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319770, Length=402, Percent_Identity=24.6268656716418, Blast_Score=67, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6319783, Length=389, Percent_Identity=25.706940874036, Blast_Score=63, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 51461; Mature: 51330

Theoretical pI: Translated: 10.94; Mature: 10.94

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRSPLAMQSAIISYTLTVA
CCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
MFIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQLVVFRVIQGIGGAMMMPVA
HHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHH
RLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVTWATWHWIFLINIPIGIAGLL
HHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHH
YARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELFGEKIVASWIALTVIVTSIGLLL
HHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRLGTGSVPFLMPLMLQVGFGYQAFIA
HHHHHHCCCCCCCEEEEEEEEEEEEEEEHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHH
GCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGITVIIGLMIAQFSLQSPAMAIWMLILP
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LFILGMAMSTQFTAMNTITLADLTDDNASSGNSVLAVTQQLSISLGVAVSAAVLRVYEGM
HHHHHHHHHCCHHHEEEEEEEECCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCC
EGTTTVEQFHYTFITMGIITVASAAMFMLLKTTDGNNLIKRQRKSKPNRVPSESE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRSPLAMQSAIISYTLTVA
CCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
MFIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQLVVFRVIQGIGGAMMMPVA
HHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHH
RLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVTWATWHWIFLINIPIGIAGLL
HHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHH
YARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELFGEKIVASWIALTVIVTSIGLLL
HHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRLGTGSVPFLMPLMLQVGFGYQAFIA
HHHHHHCCCCCCCEEEEEEEEEEEEEEEHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHH
GCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGITVIIGLMIAQFSLQSPAMAIWMLILP
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LFILGMAMSTQFTAMNTITLADLTDDNASSGNSVLAVTQQLSISLGVAVSAAVLRVYEGM
HHHHHHHHHCCHHHEEEEEEEECCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCC
EGTTTVEQFHYTFITMGIITVASAAMFMLLKTTDGNNLIKRQRKSKPNRVPSESE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]