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The map label for this gene is gltX [H]

Identifier: 82544866

GI number: 82544866

Start: 2421277

End: 2422692

Strand: Reverse

Name: gltX [H]

Synonym: SBO_2426

Alternate gene names: 82544866

Gene position: 2422692-2421277 (Counterclockwise)

Preceding gene: 82544868

Following gene: 82544863

Centisome position: 53.6

GC content: 52.9

Gene sequence:

>1416_bases
ATGAAAATCAAAACTCGCTTCGCGCCAAGCCCAACAGGCTATCTGCACGTTGGCGGCGCGCGTACTGCCCTTTACTCCTG
GCTTTTTGCACGTAACCACGGCGGTGAGTTCGTGCTGCGTATTGAAGACACCGATCTTGAGCGTTCCACGCCGGAAGCTA
TCGAAGCCATTATGGATGGCATGAACTGGCTGAGCCTGGAGTGGGATGAAGGTCCGTACTACCAGACCAAACGTTTTGAT
CGCTACAACGCGGTGATCGATCAGATGCTGGAAGAGGGCACTGCTTATAAATGCTATTGCTCTAAAGAGCGCCTGGAAGC
GCTGCGCGAAGAGCAAATGGCGAAAGGTGAGAAGCCGCGTTATGACGGTCGCTGCCGCCACAGCCATGAGCATCACGCTG
ATGATGAACCGTGCGTTGTACGTTTTGCTAACCCGCAGGAAGGTTCTGTTGTTTTTGACGATCAGATCCGTGGTCCGATC
GAGTTCAGCAACCAGGAACTGGACGATCTTATTATTCGCCGTACCGATGGTTCCCCAACCTATAACTTCTGTGTGGTTGT
CGATGACTGGGATATGGAAATCACCCACGTTATCCGTGGCGAAGACCATATCAACAACACGCCGCGCCAGATCAACATTC
TTAAGGCCCTGAAAGCGCCGGTGCCGGTTTACGCGCACGTTTCTATGATCAATGGCGATGACGGTAAAAAACTGTCCAAA
CGTCACGGGGCAGTCAGCGTAATGCAGTATCGTGATGACGGTTATTTGCCAGAAGCACTGCTGAACTATCTGGTGCGTCT
GGGCTGGTCCCACGGCGATCAGGAAATCTTCACTCGTGAAGAGATGATCAAATACTTCACTTTGAATGCCGTCAGCAAAT
CTGCCAGTGCGTTCAACACCGACAAGCTGCTGTGGCTGAACCATCACTACATTAACGCGCTGCCGCCGGAGTATGTTGCT
ACTCACTTACAGTGGCACATTGAGCAGGAAAATATCGATACCCGTAACGGCCCGCAGCTGGCTGATCTGGTGAAACTGCT
GGGCGAACGCTGCAAGACGCTGAAAGAGATGGCACAGAGCTGCCGTTATTTCTACGAAGATTTTGCTGAGTTCGATGCCG
ATGCGCGAAAAAAACATCTGCGTCCGGTAGCGCGTCAGCCGCTGGAAGTGGTTCGTGACAAACTGGCCGCGATTACTGAC
TGGCCCGCTGAAAACGTTCATCACGCTATTCAGGCGACGGCGGATGAGCTGGAAGTGGGTATGGGTAAAGTTGGTATGCC
GCTGCGTGTCGCCGTAACCGGTGCGGGGCAGTCTCCGGCACTGGATGTTACCGTTCACGCAATTGGTAGGACCCGCAGTA
TTGAGCGTATCAACAAAGCGCTGGCTTTTATTGCTGAACGCGAAAACCAGCAGTAA

Upstream 100 bases:

>100_bases
AACTACATTGAGGAATCAGGCGGGAGTGATAGAATATCGCCCACTCAATTTTTCCAGGATTTGCCGGTTGTCGGCATCTT
TCTAAACGTAAGGCCATTTC

Downstream 100 bases:

>100_bases
TTAGCGCATAAAAGATAAACGGCAGGAGATAATATCCTGCCGTTTTTTATTTATGCTGATTCGTCAATATCGAGACGGGC
GAGGAAATTGCGAATTCCTT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDTDLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFD
RYNAVIDQMLEEGTAYKCYCSKERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALKAPVPVYAHVSMINGDDGKKLSK
RHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEIFTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVA
THLQWHIEQENIDTRNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADARKKHLRPVARQPLEVVRDKLAAITD
WPAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGRTRSIERINKALAFIAERENQQ

Sequences:

>Translated_471_residues
MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDTDLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFD
RYNAVIDQMLEEGTAYKCYCSKERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALKAPVPVYAHVSMINGDDGKKLSK
RHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEIFTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVA
THLQWHIEQENIDTRNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADARKKHLRPVARQPLEVVRDKLAAITD
WPAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGRTRSIERINKALAFIAERENQQ
>Mature_471_residues
MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDTDLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFD
RYNAVIDQMLEEGTAYKCYCSKERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALKAPVPVYAHVSMINGDDGKKLSK
RHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEIFTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVA
THLQWHIEQENIDTRNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADARKKHLRPVARQPLEVVRDKLAAITD
WPAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGRTRSIERINKALAFIAERENQQ

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=494, Percent_Identity=30.9716599190283, Blast_Score=224, Evalue=1e-58,
Organism=Escherichia coli, GI1788743, Length=471, Percent_Identity=99.1507430997877, Blast_Score=979, Evalue=0.0,
Organism=Escherichia coli, GI226510925, Length=301, Percent_Identity=34.21926910299, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1786895, Length=122, Percent_Identity=28.6885245901639, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71993394, Length=488, Percent_Identity=29.7131147540984, Blast_Score=211, Evalue=5e-55,
Organism=Saccharomyces cerevisiae, GI6324540, Length=498, Percent_Identity=32.3293172690763, Blast_Score=239, Evalue=6e-64,
Organism=Saccharomyces cerevisiae, GI6321192, Length=230, Percent_Identity=26.9565217391304, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324742, Length=130, Percent_Identity=32.3076923076923, Blast_Score=81, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24665321, Length=491, Percent_Identity=30.3462321792261, Blast_Score=239, Evalue=4e-63,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 53881; Mature: 53881

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDTDLERSTPEAIEAIMDG
CCCCCCCCCCCCCEEEECCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHCC
MNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSKERLEALREEQMAKGEKPR
CCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHCCCCCC
YDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPIEFSNQELDDLIIRRTDGSPT
CCCCCCCCCCCCCCCCCEEEEECCCCCCCEEECCCCCCCCCCCCCCCCHHEEEECCCCCC
YNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALKAPVPVYAHVSMINGDDGKKLSK
EEEEEEEECCCCEEEEEECCCHHCCCCCHHHHHHHHHCCCCCEEEEEEEEECCCCHHHHH
RHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEIFTREEMIKYFTLNAVSKSASAFNT
HCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
DKLLWLNHHYINALPPEYVATHLQWHIEQENIDTRNGPQLADLVKLLGERCKTLKEMAQS
CEEEEEECCCCCCCCHHHHHHHHHEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
CRYFYEDFAEFDADARKKHLRPVARQPLEVVRDKLAAITDWPAENVHHAIQATADELEVG
HHHHHHHHHHCCHHHHHHHCCHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHC
MGKVGMPLRVAVTGAGQSPALDVTVHAIGRTRSIERINKALAFIAERENQQ
CCCCCCCEEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDTDLERSTPEAIEAIMDG
CCCCCCCCCCCCCEEEECCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHCC
MNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSKERLEALREEQMAKGEKPR
CCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHCCCCCC
YDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPIEFSNQELDDLIIRRTDGSPT
CCCCCCCCCCCCCCCCCEEEEECCCCCCCEEECCCCCCCCCCCCCCCCHHEEEECCCCCC
YNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALKAPVPVYAHVSMINGDDGKKLSK
EEEEEEEECCCCEEEEEECCCHHCCCCCHHHHHHHHHCCCCCEEEEEEEEECCCCHHHHH
RHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEIFTREEMIKYFTLNAVSKSASAFNT
HCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
DKLLWLNHHYINALPPEYVATHLQWHIEQENIDTRNGPQLADLVKLLGERCKTLKEMAQS
CEEEEEECCCCCCCCHHHHHHHHHEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
CRYFYEDFAEFDADARKKHLRPVARQPLEVVRDKLAAITDWPAENVHHAIQATADELEVG
HHHHHHHHHHCCHHHHHHHCCHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHC
MGKVGMPLRVAVTGAGQSPALDVTVHAIGRTRSIERINKALAFIAERENQQ
CCCCCCCEEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA