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The map label for this gene is fadI [H]

Identifier: 82544823

GI number: 82544823

Start: 2369484

End: 2370794

Strand: Reverse

Name: fadI [H]

Synonym: SBO_2380

Alternate gene names: 82544823

Gene position: 2370794-2369484 (Counterclockwise)

Preceding gene: 82544824

Following gene: 82544822

Centisome position: 52.45

GC content: 55.61

Gene sequence:

>1311_bases
ATGGGTCAGGTTTTACCGCTGGTTACCCGCCAGGGCGATCGTATCGCCATTGTTAGCGGTTTACGTACGCCTTTTGCCCG
CCAGGCGACGGCTTTTCATGGCATTCCCGCGGTTGATTTAGGGAAGATGGTGGTAGGCGAACTGCTGGCACGCAGCGAGA
TCCCCGCTGAAGTGATTGAACAGCTGGTCTTTGGTCAGGTCGTACAAATGCCTGAAGCCCCCAACATTGCGCGTGAAATT
GTTCTCGGTACCGGAATGAATGTGCATACCGATGCTTACAGCGTCAGCCGCGCTTGCGCTACCAGTTTCCAGGCGGTTGC
AAACGTCGCAGAAAGCCTGATGGCGGGAACTATCCGAGCGGGGATTGCCGGTGGGGCAGATTCCTCTTCCGTATTGCCAA
TTGGCGTCAGTAAAAAACTGGCGCGCGTGCTGGTTGATGTCAACAAAGCTCGAACCATGAGCCAGCGCCTGAAACTCTTC
TCTCGCCTGCGTTTGCGCGATTTAATGCCCGTACCGCCTGCAGTAGCAGAATATTCTACCGGCTTGCGGATGGGTGACAC
CGCAGAGCAAATGGCGAAAACCTACGGCATCACCCGAGAACAGCAAGATGCATTAGCGCACCGTTCGCATCAGCGTGCCG
CTCAGGCATGGTCAGACGGAAAACTCAAAGAAGAGGTGATGACTGCCTTTATCCCTCCTTATAAACAACCGCTTGTCGAA
GACAACAATATTCGCGGTAATTCCTCGCTTGCCGATTACGCAAAGCTGCGCCCGGCGTTTGATCGCAAACACGGAACGGT
AACGGCGGCAAACAGTACGCCGCTGACCGATGGCGCGGCAGCGGTGATCCTGATGACTGAATCCCGGGCGAAAGAATTAG
GGCTGGTGCCGCTGGGGTATCTGCGCAGCTACGCATTTACTGCGATTGATGTCTGGCAGGACATGTTGTTCGGTCCGGCC
TGGTCAACACCGCTGGCGCTGGAACGTGCCGGTTTGACAATGAGCGATCTGACATTGATCGATATGCACGAAGCCTTTGC
AGCTCAGACACTGGCGAATATTCAGTTGCTGGGTAGTGAACGTTTTGCTTGTGAAGTACTGGGGCGTGCACATGCCACTG
GCGAAGTGGACGATAGCAAATTTAACGTGCTTGGCGGTTCGATTGCTTACGGGCATCCCTTCGCGGCGACCGGCGCGTGG
ATGATTACCCAGACATTGCATGAACTTCGCCGTCGCGGCGGTGGATTTGGTTTAGTTACCGCCTGTGCTGCCGGTGGGCT
TGGCGCGGCAATGGTTCTGGAGGCGGAATAA

Upstream 100 bases:

>100_bases
GCTCCCACTTTCCTTTTTCTCCGCTTGGCTAAATGTAAAAAAATGGTTAAGACTATAATCAGGTCAGACCACTTTATTTA
TTTTTTTACAGGGGAGTGTT

Downstream 100 bases:

>100_bases
TGGAAATGGCATCAGCGTTTACTCTTAATGTTCGTCTGGACAACATTGCTATCATCACCATCGACGTACCGGATGAGAAA
ATGAATACCCTGAAGGCGGA

Product: 3-ketoacyl-CoA thiolase

Products: NA

Alternate protein names: ACSs; Acetyl-CoA acyltransferase; Acyl-CoA ligase; Beta-ketothiolase; Fatty acid oxidation complex subunit beta [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMVVGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREI
VLGTGMNVHTDAYSVSRACATSFQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWSDGKLKEEVMTAFIPPYKQPLVE
DNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLTDGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLFGPA
WSTPLALERAGLTMSDLTLIDMHEAFAAQTLANIQLLGSERFACEVLGRAHATGEVDDSKFNVLGGSIAYGHPFAATGAW
MITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE

Sequences:

>Translated_436_residues
MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMVVGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREI
VLGTGMNVHTDAYSVSRACATSFQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWSDGKLKEEVMTAFIPPYKQPLVE
DNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLTDGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLFGPA
WSTPLALERAGLTMSDLTLIDMHEAFAAQTLANIQLLGSERFACEVLGRAHATGEVDDSKFNVLGGSIAYGHPFAATGAW
MITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE
>Mature_435_residues
GQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMVVGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIV
LGTGMNVHTDAYSVSRACATSFQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLFS
RLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWSDGKLKEEVMTAFIPPYKQPLVED
NNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLTDGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLFGPAW
STPLALERAGLTMSDLTLIDMHEAFAAQTLANIQLLGSERFACEVLGRAHATGEVDDSKFNVLGGSIAYGHPFAATGAWM
ITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE

Specific function: Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4504327, Length=421, Percent_Identity=41.3301662707838, Blast_Score=332, Evalue=3e-91,
Organism=Homo sapiens, GI4557237, Length=423, Percent_Identity=29.5508274231678, Blast_Score=203, Evalue=3e-52,
Organism=Homo sapiens, GI4501853, Length=430, Percent_Identity=31.8604651162791, Blast_Score=192, Evalue=7e-49,
Organism=Homo sapiens, GI167614485, Length=424, Percent_Identity=30.188679245283, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI148539872, Length=428, Percent_Identity=29.6728971962617, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI194353979, Length=106, Percent_Identity=36.7924528301887, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI1788683, Length=436, Percent_Identity=99.0825688073395, Blast_Score=875, Evalue=0.0,
Organism=Escherichia coli, GI1788554, Length=423, Percent_Identity=35.4609929078014, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI87082165, Length=422, Percent_Identity=32.4644549763033, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI48994986, Length=432, Percent_Identity=34.7222222222222, Blast_Score=196, Evalue=4e-51,
Organism=Escherichia coli, GI1787663, Length=426, Percent_Identity=32.6291079812207, Blast_Score=181, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17551802, Length=423, Percent_Identity=38.2978723404255, Blast_Score=293, Evalue=9e-80,
Organism=Caenorhabditis elegans, GI133906874, Length=426, Percent_Identity=30.9859154929577, Blast_Score=177, Evalue=8e-45,
Organism=Caenorhabditis elegans, GI25147385, Length=420, Percent_Identity=28.3333333333333, Blast_Score=164, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17535921, Length=424, Percent_Identity=29.4811320754717, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI17535917, Length=420, Percent_Identity=24.2857142857143, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6325229, Length=438, Percent_Identity=30.365296803653, Blast_Score=170, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6322031, Length=440, Percent_Identity=27.9545454545455, Blast_Score=155, Evalue=1e-38,
Organism=Drosophila melanogaster, GI17137578, Length=430, Percent_Identity=40.6976744186046, Blast_Score=309, Evalue=2e-84,
Organism=Drosophila melanogaster, GI24655093, Length=422, Percent_Identity=28.9099526066351, Blast_Score=178, Evalue=7e-45,
Organism=Drosophila melanogaster, GI24640423, Length=425, Percent_Identity=27.0588235294118, Blast_Score=166, Evalue=4e-41,
Organism=Drosophila melanogaster, GI17648125, Length=430, Percent_Identity=29.0697674418605, Blast_Score=162, Evalue=6e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012806
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.16 [H]

Molecular weight: Translated: 46570; Mature: 46439

Theoretical pI: Translated: 7.69; Mature: 7.69

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMVVGELLARSEIPAEVIE
CCCCCCHHCCCCCEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHH
QLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATSFQAVANVAESLMAGTIRA
HHHHHHHHCCCCCCCHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLFSRLRLRDLMPVPPAVAEYST
CCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHC
GLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWSDGKLKEEVMTAFIPPYKQPLVE
CCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCC
DNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLTDGAAAVILMTESRAKELGLVPLGY
CCCCCCCCCHHHHHHHCHHHHCCCCEEEECCCCCCCCCCEEEEEEECCHHHHCCCCCHHH
LRSYAFTAIDVWQDMLFGPAWSTPLALERAGLTMSDLTLIDMHEAFAAQTLANIQLLGSE
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCHHHEEEHHHHHHHHHHHHHHHHEECCC
RFACEVLGRAHATGEVDDSKFNVLGGSIAYGHPFAATGAWMITQTLHELRRRGGGFGLVT
HHHHHHHHHHCCCCCCCCCCEEEECCCEECCCCCHHCHHHHHHHHHHHHHHCCCCCCHHH
ACAAGGLGAAMVLEAE
HHHCCCCCEEEEEECC
>Mature Secondary Structure 
GQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMVVGELLARSEIPAEVIE
CCCCCHHCCCCCEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHH
QLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATSFQAVANVAESLMAGTIRA
HHHHHHHHCCCCCCCHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLFSRLRLRDLMPVPPAVAEYST
CCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHC
GLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWSDGKLKEEVMTAFIPPYKQPLVE
CCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCC
DNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLTDGAAAVILMTESRAKELGLVPLGY
CCCCCCCCCHHHHHHHCHHHHCCCCEEEECCCCCCCCCCEEEEEEECCHHHHCCCCCHHH
LRSYAFTAIDVWQDMLFGPAWSTPLALERAGLTMSDLTLIDMHEAFAAQTLANIQLLGSE
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCHHHEEEHHHHHHHHHHHHHHHHEECCC
RFACEVLGRAHATGEVDDSKFNVLGGSIAYGHPFAATGAWMITQTLHELRRRGGGFGLVT
HHHHHHHHHHCCCCCCCCCCEEEECCCEECCCCCHHCHHHHHHHHHHHHHHCCCCCCHHH
ACAAGGLGAAMVLEAE
HHHCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA