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The map label for this gene is leuB

Identifier: 78779178

GI number: 78779178

Start: 739777

End: 740850

Strand: Reverse

Name: leuB

Synonym: PMT9312_0794

Alternate gene names: 78779178

Gene position: 740850-739777 (Counterclockwise)

Preceding gene: 78779179

Following gene: 78779177

Centisome position: 43.34

GC content: 32.31

Gene sequence:

>1074_bases
ATGAAGAATTATAAGATTGTTTTATTGTCAGGCGACGGAATTGGACCAGAGATTTCAGAAGTTTCAAAGAAAGTTTTAAA
AAAGCTTTCAAGAAAACATAATTTCAATATTGAGATTATTGAAAAATTATTTGGAGGGATAGCTTATGAAAAATATGGGA
CTCCTGCTCCAGATGAGACACTAGATCAATGCAAAAAATGTGATGCAGTACTTTTAGCATGTGTTGGAGATATTAAATAT
GACTCTCTTGCAAGAGAACTAAGACCAGAAAGTGGATTACTTAAATTAAGATCTGCCCTAGGCCTTTTCGCAAATATCAG
GCCTGTCAAAATAAGAAAATCTCTTGTAAATACAAGTACATTAAAAAAAGAAATCGTTGAGAATGTAGATCTTATTGTTG
TAAGAGAATTAATAGGCGGTATTTATTTTGGAAAGCCAAGAGGACATATAACAAATACAAAAATACCGAAAGCTTTCAAC
ACAATGGTTTATGATTCAGCCGAAATAGAGAGAATAACTGAAATAGCAATAAAAATTGCTAACCAAAGAAATAAAAAAAT
ATGTTCTGTTGATAAATCAAACGTTCTGGAAGTTAGTCAATTGTGGAGAGATACAGTTTTAAATATCACCTTAAAAGATA
AAAATATATCTTTAAGCAATATGTACGTTGATAATGCAGCAATGCAATTGGTAAGAGATCCTAGTCAATTTGATGTGATT
TTAACTAGCAACTTATTTGGTGATATTTTAAGCGATTTAGCCGCGATGTTAACTGGTTCTATTGGGATGCTTCCATCTGC
ATCTCTAAACAATAATGGACCAGGTGTTTTTGAACCTGTTCATGGTTCAGCTCCTGATATAGCTGGTAAAAACATAGCAA
ATCCTATTGCGATGCTTTTATCCGCTTCTATGATGTTAAAAATTGGATTAAATGAAGAAGAAGCGGCGAAAAATTTAGAA
ACTGCTGTTGATAAAGTTTTAGCAGAAGGATTTAGAACAGCCGATTTAGCTGATGGGTCTTCCGAAGTATTATCTTGCAG
TGAAATTGGAGATAAAATAATAGATGAAATTTAA

Upstream 100 bases:

>100_bases
CTAAGGAGTTCAAGTATTTTTAAAAAATTACCAGAATTAGCAAAAAAACTTAGACAATTAGACAAGCAATAATTTATTGT
TCTATTAAACAAAAATTTTA

Downstream 100 bases:

>100_bases
AAAAAAAAATTAATAAATAAAAAAATATTTTAAGTTGAACATAAAGTTGGCATATGACCTGTCAGAATTATCAGATATTG
TTTTTAAAAAATGTCAAAAC

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MKNYKIVLLSGDGIGPEISEVSKKVLKKLSRKHNFNIEIIEKLFGGIAYEKYGTPAPDETLDQCKKCDAVLLACVGDIKY
DSLARELRPESGLLKLRSALGLFANIRPVKIRKSLVNTSTLKKEIVENVDLIVVRELIGGIYFGKPRGHITNTKIPKAFN
TMVYDSAEIERITEIAIKIANQRNKKICSVDKSNVLEVSQLWRDTVLNITLKDKNISLSNMYVDNAAMQLVRDPSQFDVI
LTSNLFGDILSDLAAMLTGSIGMLPSASLNNNGPGVFEPVHGSAPDIAGKNIANPIAMLLSASMMLKIGLNEEEAAKNLE
TAVDKVLAEGFRTADLADGSSEVLSCSEIGDKIIDEI

Sequences:

>Translated_357_residues
MKNYKIVLLSGDGIGPEISEVSKKVLKKLSRKHNFNIEIIEKLFGGIAYEKYGTPAPDETLDQCKKCDAVLLACVGDIKY
DSLARELRPESGLLKLRSALGLFANIRPVKIRKSLVNTSTLKKEIVENVDLIVVRELIGGIYFGKPRGHITNTKIPKAFN
TMVYDSAEIERITEIAIKIANQRNKKICSVDKSNVLEVSQLWRDTVLNITLKDKNISLSNMYVDNAAMQLVRDPSQFDVI
LTSNLFGDILSDLAAMLTGSIGMLPSASLNNNGPGVFEPVHGSAPDIAGKNIANPIAMLLSASMMLKIGLNEEEAAKNLE
TAVDKVLAEGFRTADLADGSSEVLSCSEIGDKIIDEI
>Mature_357_residues
MKNYKIVLLSGDGIGPEISEVSKKVLKKLSRKHNFNIEIIEKLFGGIAYEKYGTPAPDETLDQCKKCDAVLLACVGDIKY
DSLARELRPESGLLKLRSALGLFANIRPVKIRKSLVNTSTLKKEIVENVDLIVVRELIGGIYFGKPRGHITNTKIPKAFN
TMVYDSAEIERITEIAIKIANQRNKKICSVDKSNVLEVSQLWRDTVLNITLKDKNISLSNMYVDNAAMQLVRDPSQFDVI
LTSNLFGDILSDLAAMLTGSIGMLPSASLNNNGPGVFEPVHGSAPDIAGKNIANPIAMLLSASMMLKIGLNEEEAAKNLE
TAVDKVLAEGFRTADLADGSSEVLSCSEIGDKIIDEI

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5031777, Length=360, Percent_Identity=32.7777777777778, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI28178816, Length=313, Percent_Identity=31.3099041533546, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI28178821, Length=313, Percent_Identity=31.3099041533546, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI4758582, Length=215, Percent_Identity=33.4883720930233, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI28178838, Length=215, Percent_Identity=33.4883720930233, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI28178819, Length=145, Percent_Identity=37.9310344827586, Blast_Score=90, Evalue=4e-18,
Organism=Escherichia coli, GI87081683, Length=354, Percent_Identity=49.1525423728814, Blast_Score=340, Evalue=8e-95,
Organism=Escherichia coli, GI1788101, Length=345, Percent_Identity=33.0434782608696, Blast_Score=174, Evalue=8e-45,
Organism=Escherichia coli, GI1787381, Length=411, Percent_Identity=26.0340632603406, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71986051, Length=341, Percent_Identity=32.8445747800587, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17550882, Length=362, Percent_Identity=27.9005524861878, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI25144293, Length=368, Percent_Identity=27.7173913043478, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17505779, Length=350, Percent_Identity=28.5714285714286, Blast_Score=98, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6319830, Length=365, Percent_Identity=44.6575342465753, Blast_Score=266, Evalue=2e-72,
Organism=Saccharomyces cerevisiae, GI6322097, Length=362, Percent_Identity=37.5690607734807, Blast_Score=180, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6324709, Length=354, Percent_Identity=32.7683615819209, Blast_Score=134, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6324291, Length=363, Percent_Identity=29.7520661157025, Blast_Score=125, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24643268, Length=354, Percent_Identity=32.4858757062147, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24643270, Length=354, Percent_Identity=32.4858757062147, Blast_Score=145, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24661184, Length=368, Percent_Identity=31.5217391304348, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI161078635, Length=343, Percent_Identity=27.1137026239067, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI161078637, Length=343, Percent_Identity=27.1137026239067, Blast_Score=101, Evalue=9e-22,
Organism=Drosophila melanogaster, GI161078633, Length=343, Percent_Identity=27.1137026239067, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24650122, Length=343, Percent_Identity=27.1137026239067, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI281362242, Length=345, Percent_Identity=29.2753623188406, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24648872, Length=345, Percent_Identity=29.2753623188406, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI161078639, Length=339, Percent_Identity=27.4336283185841, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI20130355, Length=237, Percent_Identity=25.7383966244726, Blast_Score=75, Evalue=7e-14,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU3_PROM9 (Q31B91)

Other databases:

- EMBL:   CP000111
- RefSeq:   YP_397290.1
- HSSP:   Q9WZ26
- ProteinModelPortal:   Q31B91
- SMR:   Q31B91
- STRING:   Q31B91
- GeneID:   3765594
- GenomeReviews:   CP000111_GR
- KEGG:   pmi:PMT9312_0794
- eggNOG:   COG0473
- HOGENOM:   HBG518924
- OMA:   MSYQIAV
- ProtClustDB:   PRK00772
- BioCyc:   PMAR74546:PMT9312_0794-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01033
- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429
- Gene3D:   G3DSA:3.40.718.10
- PANTHER:   PTHR11835
- PANTHER:   PTHR11835:SF13
- TIGRFAMs:   TIGR00169

Pfam domain/function: PF00180 Iso_dh

EC number: =1.1.1.85

Molecular weight: Translated: 38950; Mature: 38950

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00470 IDH_IMDH

Important sites: BINDING 97-97 BINDING 107-107 BINDING 135-135 BINDING 224-224

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNYKIVLLSGDGIGPEISEVSKKVLKKLSRKHNFNIEIIEKLFGGIAYEKYGTPAPDET
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHCCCCCCHHH
LDQCKKCDAVLLACVGDIKYDSLARELRPESGLLKLRSALGLFANIRPVKIRKSLVNTST
HHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LKKEIVENVDLIVVRELIGGIYFGKPRGHITNTKIPKAFNTMVYDSAEIERITEIAIKIA
HHHHHHHCCHHHHHHHHHHHEEECCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHH
NQRNKKICSVDKSNVLEVSQLWRDTVLNITLKDKNISLSNMYVDNAAMQLVRDPSQFDVI
CCCCCEEECCCCCHHHHHHHHHHHHHEEEEEECCCCCHHHEEHHHHHHHHHCCCCHHEEE
LTSNLFGDILSDLAAMLTGSIGMLPSASLNNNGPGVFEPVHGSAPDIAGKNIANPIAMLL
EEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
SASMMLKIGLNEEEAAKNLETAVDKVLAEGFRTADLADGSSEVLSCSEIGDKIIDEI
HHHHHEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKNYKIVLLSGDGIGPEISEVSKKVLKKLSRKHNFNIEIIEKLFGGIAYEKYGTPAPDET
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHCCCCCCHHH
LDQCKKCDAVLLACVGDIKYDSLARELRPESGLLKLRSALGLFANIRPVKIRKSLVNTST
HHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LKKEIVENVDLIVVRELIGGIYFGKPRGHITNTKIPKAFNTMVYDSAEIERITEIAIKIA
HHHHHHHCCHHHHHHHHHHHEEECCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHH
NQRNKKICSVDKSNVLEVSQLWRDTVLNITLKDKNISLSNMYVDNAAMQLVRDPSQFDVI
CCCCCEEECCCCCHHHHHHHHHHHHHEEEEEECCCCCHHHEEHHHHHHHHHCCCCHHEEE
LTSNLFGDILSDLAAMLTGSIGMLPSASLNNNGPGVFEPVHGSAPDIAGKNIANPIAMLL
EEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
SASMMLKIGLNEEEAAKNLETAVDKVLAEGFRTADLADGSSEVLSCSEIGDKIIDEI
HHHHHEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA