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The map label for this gene is ilvD [H]

Identifier: 78779166

GI number: 78779166

Start: 729536

End: 731209

Strand: Reverse

Name: ilvD [H]

Synonym: PMT9312_0782

Alternate gene names: 78779166

Gene position: 731209-729536 (Counterclockwise)

Preceding gene: 78779167

Following gene: 78779158

Centisome position: 42.78

GC content: 37.4

Gene sequence:

>1674_bases
ATGAATAAACTCAGATCATCTGCAATAACCCAAGGTGTGCAAAGATCCCCTAACAGATCGATGTTAAGAGCTGTTGGATT
TAATGATGAAGATTTTAATAAACCTATTGTTGGAGTTGCAAATGGATACAGCACCATAACACCATGCAATATGGGTTTAA
ATAAGTTAGCTCTAAAAGCTGAAGAGTCAATAAAAAGATCAGGTGGGATGCCTCAGATGTTTGGGACCATAACAGTAAGC
GATGGCATTTCTATGGGAACAGAGGGCATGAAATACTCCCTAGTTTCAAGAGAAGTTATTGCTGATTCAATTGAAACAGC
ATGCAATGCTCAGAGTATGGATGGAGTACTTGCTATAGGTGGATGTGACAAAAATATGCCGGGTGCCATGATTGCGATTG
CAAGAATGAATATTCCCTCAATTTTCATTTATGGAGGGACAATAAAGCCTGGGAAATTGCATGGAGAAGATCTTACTGTT
GTTAGTGCATTTGAAGCTGTTGGACAATTAACAGCCGGCAAAATTAATGAAGAAAGGCTAATCCAAGTTGAGAAAAATTG
TATTCCTGGTGCTGGTAGCTGTGGAGGAATGTTTACAGCTAATACAATGTCTGCGGTTATTGAAGTATTAGGGTTAAGTC
TTCCTCACAGTTCCACTATGGCTGCTGAAGATCTTGAAAAAGAACTAAGTGCAGATAAAAGTGCTGAGATATTAGTCTCT
GCAATAGAAAAAGATATAAGACCTCTAGACCTAATGACTAAGAAAGCATTTGAAAATGCAATATCAGTAATTATGGCAAT
TGGCGGATCAACAAATGCGGTATTGCACATCTTAGCTATTGCGAATACTGCAGGAATAGATATCAACATTAATGATTTTG
AGAGAATCAGACAAAAAGTACCCGTTATTTGTGACCTTAAACCGAGTGGTAAATATGTGACGGTGGATCTTCATAAGGCA
GGTGGGATTCCACAAGTAATGAAAATACTTTTAAATGCAGGATTAATTCATGGCGATTGCAAAAACATTGAAGGCAAAAC
CATCTCAGAATACTTACAGAATATTCCAGATAAGCCTCCAACAAATCAAAATGTCATAAGAGACATAGATGACCCTCTTT
ATAAAAAAGGACATCTAGCGATATTAAAAGGTAACTTAGCGAGCGAAGGTTCTGTAGCCAAAATTAGCGGAGTAAAAAAC
CCTGTATTAACTGGTCCAGCAAAGATTTTTGAAAGTGAAGAGGATTGTTTAAAATCGATATTAAATAACGATATCAAAGC
TGGTGATGTGGTTGTTATTAGAAACGAAGGTCCTGTAGGAGGTCCAGGCATGAGAGAAATGTTAGCTCCAACATCTGCGA
TTGTTGGTCAAGGGCTAGGAGAGAAGGTAGCTTTAATTACCGATGGCAGATTTAGCGGTGGTACCTATGGTCTTGTTGTG
GGTCACATAGCTCCAGAGGCTGCTGTTGGAGGAAATATTGCTCTAATAAAACAAGGTGATTTAATTACAGTGGATGCTGT
AAAACAACTAATTGAAGTTGATTTATCTGACGAAGAATTAGAAAAAAGAAAAAAAGATTGGGTAAAACCTATACAAAAAT
ACAAAAGAGGAATTCTTTCAAAATATTCGAGAATCGTAAGCACATCAAGTTTAGGGGCTGTTACTGATTTATAA

Upstream 100 bases:

>100_bases
ATCTGGAGAAGAAAGTTGAGCAACTTAATTCTGCAATTGCTGATGTGAGGAAGCAACTTGAAGAGTTGGGGCAATAGAAT
AAAAAAGGTTCACAAATAAT

Downstream 100 bases:

>100_bases
ATCAGCTTAAAGTTTATTTTCTTACTCAAAAAAACTTTTTATCCTATTTGCTAAATTTTCCAATCTAGCGCCGTATTTTG
GTGAGTTGCATTTTTTCCCA

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD [H]

Number of amino acids: Translated: 557; Mature: 557

Protein sequence:

>557_residues
MNKLRSSAITQGVQRSPNRSMLRAVGFNDEDFNKPIVGVANGYSTITPCNMGLNKLALKAEESIKRSGGMPQMFGTITVS
DGISMGTEGMKYSLVSREVIADSIETACNAQSMDGVLAIGGCDKNMPGAMIAIARMNIPSIFIYGGTIKPGKLHGEDLTV
VSAFEAVGQLTAGKINEERLIQVEKNCIPGAGSCGGMFTANTMSAVIEVLGLSLPHSSTMAAEDLEKELSADKSAEILVS
AIEKDIRPLDLMTKKAFENAISVIMAIGGSTNAVLHILAIANTAGIDININDFERIRQKVPVICDLKPSGKYVTVDLHKA
GGIPQVMKILLNAGLIHGDCKNIEGKTISEYLQNIPDKPPTNQNVIRDIDDPLYKKGHLAILKGNLASEGSVAKISGVKN
PVLTGPAKIFESEEDCLKSILNNDIKAGDVVVIRNEGPVGGPGMREMLAPTSAIVGQGLGEKVALITDGRFSGGTYGLVV
GHIAPEAAVGGNIALIKQGDLITVDAVKQLIEVDLSDEELEKRKKDWVKPIQKYKRGILSKYSRIVSTSSLGAVTDL

Sequences:

>Translated_557_residues
MNKLRSSAITQGVQRSPNRSMLRAVGFNDEDFNKPIVGVANGYSTITPCNMGLNKLALKAEESIKRSGGMPQMFGTITVS
DGISMGTEGMKYSLVSREVIADSIETACNAQSMDGVLAIGGCDKNMPGAMIAIARMNIPSIFIYGGTIKPGKLHGEDLTV
VSAFEAVGQLTAGKINEERLIQVEKNCIPGAGSCGGMFTANTMSAVIEVLGLSLPHSSTMAAEDLEKELSADKSAEILVS
AIEKDIRPLDLMTKKAFENAISVIMAIGGSTNAVLHILAIANTAGIDININDFERIRQKVPVICDLKPSGKYVTVDLHKA
GGIPQVMKILLNAGLIHGDCKNIEGKTISEYLQNIPDKPPTNQNVIRDIDDPLYKKGHLAILKGNLASEGSVAKISGVKN
PVLTGPAKIFESEEDCLKSILNNDIKAGDVVVIRNEGPVGGPGMREMLAPTSAIVGQGLGEKVALITDGRFSGGTYGLVV
GHIAPEAAVGGNIALIKQGDLITVDAVKQLIEVDLSDEELEKRKKDWVKPIQKYKRGILSKYSRIVSTSSLGAVTDL
>Mature_557_residues
MNKLRSSAITQGVQRSPNRSMLRAVGFNDEDFNKPIVGVANGYSTITPCNMGLNKLALKAEESIKRSGGMPQMFGTITVS
DGISMGTEGMKYSLVSREVIADSIETACNAQSMDGVLAIGGCDKNMPGAMIAIARMNIPSIFIYGGTIKPGKLHGEDLTV
VSAFEAVGQLTAGKINEERLIQVEKNCIPGAGSCGGMFTANTMSAVIEVLGLSLPHSSTMAAEDLEKELSADKSAEILVS
AIEKDIRPLDLMTKKAFENAISVIMAIGGSTNAVLHILAIANTAGIDININDFERIRQKVPVICDLKPSGKYVTVDLHKA
GGIPQVMKILLNAGLIHGDCKNIEGKTISEYLQNIPDKPPTNQNVIRDIDDPLYKKGHLAILKGNLASEGSVAKISGVKN
PVLTGPAKIFESEEDCLKSILNNDIKAGDVVVIRNEGPVGGPGMREMLAPTSAIVGQGLGEKVALITDGRFSGGTYGLVV
GHIAPEAAVGGNIALIKQGDLITVDAVKQLIEVDLSDEELEKRKKDWVKPIQKYKRGILSKYSRIVSTSSLGAVTDL

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family [H]

Homologues:

Organism=Escherichia coli, GI48994964, Length=610, Percent_Identity=39.5081967213115, Blast_Score=409, Evalue=1e-115,
Organism=Escherichia coli, GI2367371, Length=510, Percent_Identity=33.921568627451, Blast_Score=210, Evalue=2e-55,
Organism=Escherichia coli, GI1788157, Length=478, Percent_Identity=30.9623430962343, Blast_Score=210, Evalue=2e-55,
Organism=Escherichia coli, GI1786464, Length=506, Percent_Identity=33.201581027668, Blast_Score=202, Evalue=4e-53,
Organism=Saccharomyces cerevisiae, GI6322476, Length=564, Percent_Identity=46.6312056737589, Blast_Score=506, Evalue=1e-144,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558 [H]

Pfam domain/function: PF00920 ILVD_EDD [H]

EC number: =4.2.1.9 [H]

Molecular weight: Translated: 59062; Mature: 59062

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00886 ILVD_EDD_1 ; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKLRSSAITQGVQRSPNRSMLRAVGFNDEDFNKPIVGVANGYSTITPCNMGLNKLALKA
CCHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHH
EESIKRSGGMPQMFGTITVSDGISMGTEGMKYSLVSREVIADSIETACNAQSMDGVLAIG
HHHHHHCCCCCCCEEEEEECCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCEEEEC
GCDKNMPGAMIAIARMNIPSIFIYGGTIKPGKLHGEDLTVVSAFEAVGQLTAGKINEERL
CCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHH
IQVEKNCIPGAGSCGGMFTANTMSAVIEVLGLSLPHSSTMAAEDLEKELSADKSAEILVS
HEECCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHH
AIEKDIRPLDLMTKKAFENAISVIMAIGGSTNAVLHILAIANTAGIDININDFERIRQKV
HHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEEEECCCCEEEECHHHHHHHHHC
PVICDLKPSGKYVTVDLHKAGGIPQVMKILLNAGLIHGDCKNIEGKTISEYLQNIPDKPP
CEEEEECCCCCEEEEEECCCCCCHHHHHHHHHCCEEECCCCCCCCCHHHHHHHHCCCCCC
TNQNVIRDIDDPLYKKGHLAILKGNLASEGSVAKISGVKNPVLTGPAKIFESEEDCLKSI
CCCHHHHHCCCHHHCCCCEEEEECCCCCCCCEEEECCCCCCCCCCHHHHHCCHHHHHHHH
LNNDIKAGDVVVIRNEGPVGGPGMREMLAPTSAIVGQGLGEKVALITDGRFSGGTYGLVV
HCCCCCCCCEEEEECCCCCCCCCHHHHHCCHHHHHHCCCCCEEEEEECCCCCCCEEEEEE
GHIAPEAAVGGNIALIKQGDLITVDAVKQLIEVDLSDEELEKRKKDWVKPIQKYKRGILS
ECCCCCHHCCCCEEEEECCCEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KYSRIVSTSSLGAVTDL
HHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNKLRSSAITQGVQRSPNRSMLRAVGFNDEDFNKPIVGVANGYSTITPCNMGLNKLALKA
CCHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHH
EESIKRSGGMPQMFGTITVSDGISMGTEGMKYSLVSREVIADSIETACNAQSMDGVLAIG
HHHHHHCCCCCCCEEEEEECCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCEEEEC
GCDKNMPGAMIAIARMNIPSIFIYGGTIKPGKLHGEDLTVVSAFEAVGQLTAGKINEERL
CCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHH
IQVEKNCIPGAGSCGGMFTANTMSAVIEVLGLSLPHSSTMAAEDLEKELSADKSAEILVS
HEECCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHH
AIEKDIRPLDLMTKKAFENAISVIMAIGGSTNAVLHILAIANTAGIDININDFERIRQKV
HHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEEEECCCCEEEECHHHHHHHHHC
PVICDLKPSGKYVTVDLHKAGGIPQVMKILLNAGLIHGDCKNIEGKTISEYLQNIPDKPP
CEEEEECCCCCEEEEEECCCCCCHHHHHHHHHCCEEECCCCCCCCCHHHHHHHHCCCCCC
TNQNVIRDIDDPLYKKGHLAILKGNLASEGSVAKISGVKNPVLTGPAKIFESEEDCLKSI
CCCHHHHHCCCHHHCCCCEEEEECCCCCCCCEEEECCCCCCCCCCHHHHHCCHHHHHHHH
LNNDIKAGDVVVIRNEGPVGGPGMREMLAPTSAIVGQGLGEKVALITDGRFSGGTYGLVV
HCCCCCCCCEEEEECCCCCCCCCHHHHHCCHHHHHHCCCCCEEEEEECCCCCCCEEEEEE
GHIAPEAAVGGNIALIKQGDLITVDAVKQLIEVDLSDEELEKRKKDWVKPIQKYKRGILS
ECCCCCHHCCCCEEEEECCCEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KYSRIVSTSSLGAVTDL
HHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA