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The map label for this gene is sphR [H]

Identifier: 78779101

GI number: 78779101

Start: 666279

End: 667007

Strand: Direct

Name: sphR [H]

Synonym: PMT9312_0717

Alternate gene names: 78779101

Gene position: 666279-667007 (Clockwise)

Preceding gene: 78779100

Following gene: 78779102

Centisome position: 38.98

GC content: 29.77

Gene sequence:

>729_bases
ATGGCTATTAAAGATCATAAAAGTTTGCAAGACTCAAGAATTCTTCTTGTAGAGGATGATAAGAGTATTAGGCTTACTGT
CAGTGAAACCTTGATCAGCGAAGGTTTCAAAGTATCAAGTTTCAAAGACGGTTTAAGTGCCTTAGATTTTATTAATAATG
ATATTAAAAAAGATGTTGACCTAATAATTCTAGATTTAATGTTGCCAGGACTAAATGGATTAGAGTTATGTAGAAAAATA
AGAAATGATGAAGACTATACACCCATACTAATTTTGAGTGCTAAAGATAATGAATCAGACAGGGTTCTTGGATTAGAGGT
TGGTGCAGATGATTATTTAACAAAACCTTTTGGTTTAAATGAATTAATTGCTAGATCTAGAGCATTAATAAGAAGATCTA
AACGTAATAAAAAATATATAGAAAACTCAAAAACTGTTCTCGAGTTTAATCATATAAAAATGTTCTTGGAAGAATGTAGG
GTAACTTCTTTTGATAGAGAAATAACATTATCTCCAAAAGAATTTAAATTATTAGAGTTATTTATGAAAAATCCAAAAAG
AGTATGGTCAAGGGATTTAATACTTGAAAAAATATGGGAAATTGACTTTATTGGTGATACTAAAACTGTAGATGTTCATG
TTAGGTGGCTCAGAGAGAAATTAGAAGAAGATCCCTCAGCTCCAAAGTTTCTTAAAACTGTAAGAGGTTTTGGATATAAG
TTTGGATGA

Upstream 100 bases:

>100_bases
ATAAGATCAAATAAAAAAATAAGCATTGAATGTAAAGTAAATATGAAATAATTAGATCAAATAATTTTGATTATATAAAA
TAAAATTGTTGGAACTTTAA

Downstream 100 bases:

>100_bases
AAATGAAAACACTACAAGAAGTATTATTTTTTTTACATCAGAAGTGGAAAATAAAAGTCAAGCATTTTTCTCAATCAAAA
GAAAAAAAATTTGAAGTTGA

Product: two component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 242; Mature: 241

Protein sequence:

>242_residues
MAIKDHKSLQDSRILLVEDDKSIRLTVSETLISEGFKVSSFKDGLSALDFINNDIKKDVDLIILDLMLPGLNGLELCRKI
RNDEDYTPILILSAKDNESDRVLGLEVGADDYLTKPFGLNELIARSRALIRRSKRNKKYIENSKTVLEFNHIKMFLEECR
VTSFDREITLSPKEFKLLELFMKNPKRVWSRDLILEKIWEIDFIGDTKTVDVHVRWLREKLEEDPSAPKFLKTVRGFGYK
FG

Sequences:

>Translated_242_residues
MAIKDHKSLQDSRILLVEDDKSIRLTVSETLISEGFKVSSFKDGLSALDFINNDIKKDVDLIILDLMLPGLNGLELCRKI
RNDEDYTPILILSAKDNESDRVLGLEVGADDYLTKPFGLNELIARSRALIRRSKRNKKYIENSKTVLEFNHIKMFLEECR
VTSFDREITLSPKEFKLLELFMKNPKRVWSRDLILEKIWEIDFIGDTKTVDVHVRWLREKLEEDPSAPKFLKTVRGFGYK
FG
>Mature_241_residues
AIKDHKSLQDSRILLVEDDKSIRLTVSETLISEGFKVSSFKDGLSALDFINNDIKKDVDLIILDLMLPGLNGLELCRKIR
NDEDYTPILILSAKDNESDRVLGLEVGADDYLTKPFGLNELIARSRALIRRSKRNKKYIENSKTVLEFNHIKMFLEECRV
TSFDREITLSPKEFKLLELFMKNPKRVWSRDLILEKIWEIDFIGDTKTVDVHVRWLREKLEEDPSAPKFLKTVRGFGYKF
G

Specific function: Member of the two-component regulatory system sphR/sphS. Response regulator. Involved in inducible production of alkaline phosphatase in response to phosphate limitation as it is directly involved in the regulation of phoA transcription in response to pho

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=232, Percent_Identity=39.2241379310345, Blast_Score=154, Evalue=4e-39,
Organism=Escherichia coli, GI1790860, Length=233, Percent_Identity=36.9098712446352, Blast_Score=154, Evalue=4e-39,
Organism=Escherichia coli, GI1789809, Length=233, Percent_Identity=33.4763948497854, Blast_Score=150, Evalue=5e-38,
Organism=Escherichia coli, GI1786784, Length=227, Percent_Identity=40.0881057268723, Blast_Score=150, Evalue=9e-38,
Organism=Escherichia coli, GI87082012, Length=229, Percent_Identity=38.8646288209607, Blast_Score=149, Evalue=1e-37,
Organism=Escherichia coli, GI1789402, Length=232, Percent_Identity=40.948275862069, Blast_Score=147, Evalue=5e-37,
Organism=Escherichia coli, GI1790863, Length=236, Percent_Identity=33.8983050847458, Blast_Score=135, Evalue=2e-33,
Organism=Escherichia coli, GI1788394, Length=232, Percent_Identity=33.6206896551724, Blast_Score=134, Evalue=6e-33,
Organism=Escherichia coli, GI1786911, Length=233, Percent_Identity=32.1888412017167, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1790552, Length=227, Percent_Identity=33.4801762114537, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI2367329, Length=232, Percent_Identity=35.3448275862069, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1787375, Length=229, Percent_Identity=31.0043668122271, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1787229, Length=228, Percent_Identity=31.140350877193, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI145693140, Length=235, Percent_Identity=29.7872340425532, Blast_Score=94, Evalue=6e-21,
Organism=Escherichia coli, GI1790299, Length=116, Percent_Identity=30.1724137931034, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788550, Length=144, Percent_Identity=29.8611111111111, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1787487, Length=110, Percent_Identity=35.4545454545455, Blast_Score=67, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 28111; Mature: 27980

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIKDHKSLQDSRILLVEDDKSIRLTVSETLISEGFKVSSFKDGLSALDFINNDIKKDVD
CCCCCCCCCCCCEEEEEECCCCEEEEEHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
LIILDLMLPGLNGLELCRKIRNDEDYTPILILSAKDNESDRVLGLEVGADDYLTKPFGLN
HHHHHHHHCCCCHHHHHHHHCCCCCCCCEEEEEECCCCCCCEEEEEECCCHHCCCCCCHH
ELIARSRALIRRSKRNKKYIENSKTVLEFNHIKMFLEECRVTSFDREITLSPKEFKLLEL
HHHHHHHHHHHHHHCCHHHHHCCCEEEEHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHH
FMKNPKRVWSRDLILEKIWEIDFIGDTKTVDVHVRWLREKLEEDPSAPKFLKTVRGFGYK
HHHCHHHHHHHHHHHHHHHHEEECCCCEEEEHHHHHHHHHHHCCCCCHHHHHHHHHCCCC
FG
CC
>Mature Secondary Structure 
AIKDHKSLQDSRILLVEDDKSIRLTVSETLISEGFKVSSFKDGLSALDFINNDIKKDVD
CCCCCCCCCCCEEEEEECCCCEEEEEHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
LIILDLMLPGLNGLELCRKIRNDEDYTPILILSAKDNESDRVLGLEVGADDYLTKPFGLN
HHHHHHHHCCCCHHHHHHHHCCCCCCCCEEEEEECCCCCCCEEEEEECCCHHCCCCCCHH
ELIARSRALIRRSKRNKKYIENSKTVLEFNHIKMFLEECRVTSFDREITLSPKEFKLLEL
HHHHHHHHHHHHHHCCHHHHHCCCEEEEHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHH
FMKNPKRVWSRDLILEKIWEIDFIGDTKTVDVHVRWLREKLEEDPSAPKFLKTVRGFGYK
HHHCHHHHHHHHHHHHHHHHEEECCCCEEEEHHHHHHHHHHHCCCCCHHHHHHHHHCCCC
FG
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8497200; 8157591 [H]