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The map label for this gene is prfC

Identifier: 78779048

GI number: 78779048

Start: 625590

End: 627227

Strand: Direct

Name: prfC

Synonym: PMT9312_0663

Alternate gene names: 78779048

Gene position: 625590-627227 (Clockwise)

Preceding gene: 78779047

Following gene: 78779049

Centisome position: 36.6

GC content: 35.41

Gene sequence:

>1638_bases
ATGACCTTAGGTACTAAAATTTTAAATAATAAAGATATTCTGGATGCTGTAAATAAACGAAGAAACTTTGCAATTATTTC
GCATCCAGATGCGGGCAAAACAACGCTTACTGAGAAGCTTCTTTTGTATGGAGGAGCCATTCAGCAGGCAGGAGCAGTAA
AGGCTAGAGGTAATCAGAGAAAAGCTACTTCAGACTGGATGGAACTTGAGAAACAAAGAGGTATTTCAATTACTTCAACT
GTATTGCAATTTGAATATGAAAGATCAGTAATTAATCTATTAGATACACCAGGGCACCAAGATTTCTCAGAAGATACTTA
TAGAACATTAGCGGCTGCTGATAATGCAGTTATGCTGGAAGATGCTGCTAAGGGGCTAGAACCCCAAACTAGAAAATTGT
TTGAAGTGTGCAAGATGAGAAAAATACCAATATTTACTTTCATAAATAAAATGGACAGGGCTGGAAGAGAGCCATTTTCT
TTACTTGATGAAATCGAATCAGAACTAGGATTAAACACTTTACCGATTAACTGGCCAATTGGAAGTGGAGAGGAATTTAG
GGGAGTTATTGATAGGTTTTCAAGAGAGGTGATTTTATTTGATAAAGCAGTAAGAGGAAAACAATCGAATGAGAAGAGGT
TAAGTCTTGAAGATAAAGACCTATCAAAATATGTAGAGAAAGAGTTGCTTGAGATATCACTTGAAGAATTGGAGGTTCTT
GATGAGGCAGGATCGAAATTTGAAGAAGAAAACGTATTTAATGGCTCCTTAACGCCTGTTTTCTTTGGATCTGCCATGAC
TAATTTTGGTGTAAGACCATTTTTAGATAGTTTTCTAAAAATGGCTCAAAAACCAACGTCAAGAAATAGTAATAAAGGGG
ATATTGAACCTGCAAGTAATGAATTTAGTGGGTTTGTTTTTAAGCTTCAAGCAAACATGGATCCAAAGCATAGAGATAGA
GTTGCTTTTATAAGAGTTTGTAGTGGGAAATTTGAAAAGGACATGTCAGTTAAACATTCCAGAACTGGGAAAACAATAAG
ATTATCAAGACCACAAAAAATATTTGGACAAGACAGAGAAGTGGTTGATGATGCGTATCCTGGTGATGTTATTGGCTTAA
ATAATCCAGGAATGTTTTCTATTGGAGATACACTTTACACAGGTACGCATACTGAGTATGAGGGCATACCATCCTTTAGT
CCTGAAATATTTAGCTGGTTGAGAAATCCCAATCCTTCAGCATTTAAAAACTTTAGAAAGGGTGTGAATGAACTTCGTGA
AGAAGGTGCTGTTCAGATTCTTTATGACTTTGATGAGAGTAAAAGAGATCCTATACTTGCAGCCGTTGGTCAATTACAGT
TGGAAGTAGTAACTCACAGGTTAAAAAATGAATATGGAGTAGATGCAAATCTTGAATCAATGCCATATCAATTGGCTAGA
TGGGTTTCTGATGGATGGCCAGCTATTGAAGAACTAGGCAGAATTTTCAATTGTAAAATAGTTAAAGATTGTTGGAATAG
ACCAGTTATTCTTTTTAAAAACGAGTGGAATCTAAATCAATTTGTTGAAGATAACAATCACTTAAATTTAAACAAAGTTG
CTCCTGTTGTTAGTGGAGTTGAGCCAATTGTTTTATAA

Upstream 100 bases:

>100_bases
ATGATCTATCTTCTTTAGAAGTGTTTGAAGATGGCGATAAATATTTAATACTCTATGGTAATGTTTAAGAACCAAGTTTA
AGGGCCTTAATATTAGATAA

Downstream 100 bases:

>100_bases
AGCCTTAATGGATTATAAAATTGGTTAATCTGTTAGTATTGGTAAAAAATTTTGAATTCAAACAATAGTCAAAACAATAG
TGAAAAAACACCTTATGAAA

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 545; Mature: 544

Protein sequence:

>545_residues
MTLGTKILNNKDILDAVNKRRNFAIISHPDAGKTTLTEKLLLYGGAIQQAGAVKARGNQRKATSDWMELEKQRGISITST
VLQFEYERSVINLLDTPGHQDFSEDTYRTLAAADNAVMLEDAAKGLEPQTRKLFEVCKMRKIPIFTFINKMDRAGREPFS
LLDEIESELGLNTLPINWPIGSGEEFRGVIDRFSREVILFDKAVRGKQSNEKRLSLEDKDLSKYVEKELLEISLEELEVL
DEAGSKFEEENVFNGSLTPVFFGSAMTNFGVRPFLDSFLKMAQKPTSRNSNKGDIEPASNEFSGFVFKLQANMDPKHRDR
VAFIRVCSGKFEKDMSVKHSRTGKTIRLSRPQKIFGQDREVVDDAYPGDVIGLNNPGMFSIGDTLYTGTHTEYEGIPSFS
PEIFSWLRNPNPSAFKNFRKGVNELREEGAVQILYDFDESKRDPILAAVGQLQLEVVTHRLKNEYGVDANLESMPYQLAR
WVSDGWPAIEELGRIFNCKIVKDCWNRPVILFKNEWNLNQFVEDNNHLNLNKVAPVVSGVEPIVL

Sequences:

>Translated_545_residues
MTLGTKILNNKDILDAVNKRRNFAIISHPDAGKTTLTEKLLLYGGAIQQAGAVKARGNQRKATSDWMELEKQRGISITST
VLQFEYERSVINLLDTPGHQDFSEDTYRTLAAADNAVMLEDAAKGLEPQTRKLFEVCKMRKIPIFTFINKMDRAGREPFS
LLDEIESELGLNTLPINWPIGSGEEFRGVIDRFSREVILFDKAVRGKQSNEKRLSLEDKDLSKYVEKELLEISLEELEVL
DEAGSKFEEENVFNGSLTPVFFGSAMTNFGVRPFLDSFLKMAQKPTSRNSNKGDIEPASNEFSGFVFKLQANMDPKHRDR
VAFIRVCSGKFEKDMSVKHSRTGKTIRLSRPQKIFGQDREVVDDAYPGDVIGLNNPGMFSIGDTLYTGTHTEYEGIPSFS
PEIFSWLRNPNPSAFKNFRKGVNELREEGAVQILYDFDESKRDPILAAVGQLQLEVVTHRLKNEYGVDANLESMPYQLAR
WVSDGWPAIEELGRIFNCKIVKDCWNRPVILFKNEWNLNQFVEDNNHLNLNKVAPVVSGVEPIVL
>Mature_544_residues
TLGTKILNNKDILDAVNKRRNFAIISHPDAGKTTLTEKLLLYGGAIQQAGAVKARGNQRKATSDWMELEKQRGISITSTV
LQFEYERSVINLLDTPGHQDFSEDTYRTLAAADNAVMLEDAAKGLEPQTRKLFEVCKMRKIPIFTFINKMDRAGREPFSL
LDEIESELGLNTLPINWPIGSGEEFRGVIDRFSREVILFDKAVRGKQSNEKRLSLEDKDLSKYVEKELLEISLEELEVLD
EAGSKFEEENVFNGSLTPVFFGSAMTNFGVRPFLDSFLKMAQKPTSRNSNKGDIEPASNEFSGFVFKLQANMDPKHRDRV
AFIRVCSGKFEKDMSVKHSRTGKTIRLSRPQKIFGQDREVVDDAYPGDVIGLNNPGMFSIGDTLYTGTHTEYEGIPSFSP
EIFSWLRNPNPSAFKNFRKGVNELREEGAVQILYDFDESKRDPILAAVGQLQLEVVTHRLKNEYGVDANLESMPYQLARW
VSDGWPAIEELGRIFNCKIVKDCWNRPVILFKNEWNLNQFVEDNNHLNLNKVAPVVSGVEPIVL

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=486, Percent_Identity=31.2757201646091, Blast_Score=202, Evalue=9e-52,
Organism=Homo sapiens, GI19923640, Length=513, Percent_Identity=26.5107212475634, Blast_Score=159, Evalue=6e-39,
Organism=Homo sapiens, GI25306283, Length=393, Percent_Identity=28.2442748091603, Blast_Score=145, Evalue=9e-35,
Organism=Homo sapiens, GI25306287, Length=485, Percent_Identity=26.5979381443299, Blast_Score=143, Evalue=3e-34,
Organism=Homo sapiens, GI157426893, Length=182, Percent_Identity=29.6703296703297, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI4503483, Length=160, Percent_Identity=32.5, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI94966754, Length=155, Percent_Identity=32.9032258064516, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI310132016, Length=134, Percent_Identity=34.3283582089552, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI310110807, Length=134, Percent_Identity=34.3283582089552, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI310123363, Length=134, Percent_Identity=34.3283582089552, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1790835, Length=471, Percent_Identity=50.5307855626327, Blast_Score=474, Evalue=1e-135,
Organism=Escherichia coli, GI1789738, Length=488, Percent_Identity=29.3032786885246, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI1788922, Length=183, Percent_Identity=31.6939890710383, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI48994988, Length=149, Percent_Identity=36.241610738255, Blast_Score=98, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17533571, Length=484, Percent_Identity=28.3057851239669, Blast_Score=189, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI17556745, Length=478, Percent_Identity=26.7782426778243, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17557151, Length=152, Percent_Identity=32.8947368421053, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17506493, Length=172, Percent_Identity=30.2325581395349, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=33.0827067669173, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=33.0827067669173, Blast_Score=71, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=484, Percent_Identity=30.1652892561983, Blast_Score=206, Evalue=8e-54,
Organism=Saccharomyces cerevisiae, GI6322359, Length=515, Percent_Identity=26.6019417475728, Blast_Score=146, Evalue=8e-36,
Organism=Saccharomyces cerevisiae, GI6324707, Length=160, Percent_Identity=33.125, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6320593, Length=160, Percent_Identity=33.125, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=138, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24582462, Length=489, Percent_Identity=30.4703476482618, Blast_Score=202, Evalue=5e-52,
Organism=Drosophila melanogaster, GI221458488, Length=568, Percent_Identity=25, Blast_Score=142, Evalue=6e-34,
Organism=Drosophila melanogaster, GI78706572, Length=174, Percent_Identity=32.183908045977, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24585709, Length=164, Percent_Identity=30.4878048780488, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24585711, Length=161, Percent_Identity=31.055900621118, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24585713, Length=161, Percent_Identity=31.055900621118, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI28574573, Length=142, Percent_Identity=31.6901408450704, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI281363316, Length=200, Percent_Identity=30, Blast_Score=69, Evalue=9e-12,
Organism=Drosophila melanogaster, GI17864358, Length=200, Percent_Identity=30, Blast_Score=69, Evalue=9e-12,
Organism=Drosophila melanogaster, GI21357743, Length=147, Percent_Identity=30.6122448979592, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU [H]

EC number: NA

Molecular weight: Translated: 61720; Mature: 61589

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLGTKILNNKDILDAVNKRRNFAIISHPDAGKTTLTEKLLLYGGAIQQAGAVKARGNQR
CCCCCCCCCCCHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCHHHHCCCCCCCCCCC
KATSDWMELEKQRGISITSTVLQFEYERSVINLLDTPGHQDFSEDTYRTLAAADNAVMLE
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCEEEEH
DAAKGLEPQTRKLFEVCKMRKIPIFTFINKMDRAGREPFSLLDEIESELGLNTLPINWPI
HHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEECCC
GSGEEFRGVIDRFSREVILFDKAVRGKQSNEKRLSLEDKDLSKYVEKELLEISLEELEVL
CCCHHHHHHHHHHCCCEEEEEHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
DEAGSKFEEENVFNGSLTPVFFGSAMTNFGVRPFLDSFLKMAQKPTSRNSNKGDIEPASN
HHHCCCHHHCCCCCCCCCCEEECHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
EFSGFVFKLQANMDPKHRDRVAFIRVCSGKFEKDMSVKHSRTGKTIRLSRPQKIFGQDRE
CCCCEEEEEECCCCCCHHHHEEEEEECCCCCCCCCCCHHCCCCCEEEECCCHHHHCCCHH
VVDDAYPGDVIGLNNPGMFSIGDTLYTGTHTEYEGIPSFSPEIFSWLRNPNPSAFKNFRK
HHHCCCCCCEEECCCCCCEEECCEEECCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHH
GVNELREEGAVQILYDFDESKRDPILAAVGQLQLEVVTHRLKNEYGVDANLESMPYQLAR
HHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
WVSDGWPAIEELGRIFNCKIVKDCWNRPVILFKNEWNLNQFVEDNNHLNLNKVAPVVSGV
HHCCCCHHHHHHHHHHCCEEEHHHHCCCEEEEECCCCHHHHHCCCCCCCHHHHHHHHCCC
EPIVL
CCCCC
>Mature Secondary Structure 
TLGTKILNNKDILDAVNKRRNFAIISHPDAGKTTLTEKLLLYGGAIQQAGAVKARGNQR
CCCCCCCCCCHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCHHHHCCCCCCCCCCC
KATSDWMELEKQRGISITSTVLQFEYERSVINLLDTPGHQDFSEDTYRTLAAADNAVMLE
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCEEEEH
DAAKGLEPQTRKLFEVCKMRKIPIFTFINKMDRAGREPFSLLDEIESELGLNTLPINWPI
HHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEECCC
GSGEEFRGVIDRFSREVILFDKAVRGKQSNEKRLSLEDKDLSKYVEKELLEISLEELEVL
CCCHHHHHHHHHHCCCEEEEEHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
DEAGSKFEEENVFNGSLTPVFFGSAMTNFGVRPFLDSFLKMAQKPTSRNSNKGDIEPASN
HHHCCCHHHCCCCCCCCCCEEECHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
EFSGFVFKLQANMDPKHRDRVAFIRVCSGKFEKDMSVKHSRTGKTIRLSRPQKIFGQDRE
CCCCEEEEEECCCCCCHHHHEEEEEECCCCCCCCCCCHHCCCCCEEEECCCHHHHCCCHH
VVDDAYPGDVIGLNNPGMFSIGDTLYTGTHTEYEGIPSFSPEIFSWLRNPNPSAFKNFRK
HHHCCCCCCEEECCCCCCEEECCEEECCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHH
GVNELREEGAVQILYDFDESKRDPILAAVGQLQLEVVTHRLKNEYGVDANLESMPYQLAR
HHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
WVSDGWPAIEELGRIFNCKIVKDCWNRPVILFKNEWNLNQFVEDNNHLNLNKVAPVVSGV
HHCCCCHHHHHHHHHHCCEEEHHHHCCCEEEEECCCCHHHHHCCCCCCCHHHHHHHHCCC
EPIVL
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA