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The map label for this gene is cocE [H]

Identifier: 78778970

GI number: 78778970

Start: 545926

End: 547506

Strand: Reverse

Name: cocE [H]

Synonym: PMT9312_0585

Alternate gene names: 78778970

Gene position: 547506-545926 (Counterclockwise)

Preceding gene: 78778971

Following gene: 78778969

Centisome position: 32.03

GC content: 33.78

Gene sequence:

>1581_bases
ATGTCTGGTTCAAGATGGTTTGACAAGTCTATAGTACTTAGAGATGGAGTAAGACTTATATCAAGAATTTGGTTACCTAA
TAGTAATGGACCATGGCCTGCATTATTAATGAGACAACCTTATGGCAGGGAAATAGCTTCAACTATTACCTATTCTCACC
CTGAGTGGTGGGCTTCCAAGGGGTATATGGTAATAATTCAAGATGTTAGAGGTATGGGTTCCTCTGAAGGCGTATTTAAT
GGTTTTTCTCAAGAAGCTAGCGACACTTCTGAAACACATAAATGGGTAAGGTCTCTAAAGGAATGTAATGGAAAACTTGG
CTTATATGGTTTTTCTTATCAAGGATTTACTCAACTAACTGGTGAATTAAATACAAAGCCGCCTGATTGTTTATCTCCAG
CAATGACTGGGATGAATATTAAGGATCATTGGTGCTCAGATGGAGGAGCATATTGGTGGCATAACAATATTGCATGGGGA
CTTCAAATCGCTGCACTAAAAATGAAAAGAGAAAATAATTTGATTGAGTGGGAAAAAATAAGAATAGCCTTAGAAAATAA
AAGTTATTTAAGGGAAGGAATTGATATTTTAAAAAGATATGATCCTAATAGCTTTGTTTTGAAATGGCTTATTAATTTAA
ATAATGCTAGCCCCTTTGAAGAATTTAACCCAATTTCAACATGGATTAAACAACCTATGTTAATTATTGGAGGACTTTGG
GATCCACATTTAAAAGGTGCCTTTGATCTTTATAAAAAATCTAAAGAAGCTGGGGGTAGTCCAGAGATTATTATTGGGAA
TGCGACACATCTAAATTGGTGGGAGGGGTCACAAGAATCTTTATTGAAATTTTTTGATAAACACTTAAAATCAGATGAAA
AATTTAGTTCTAAGAATTCACAAGGCGAGAAAAAAATATGGAATATTTCATTAAATAAATGGGAAGAATTAGATAATAAA
TTTCACCCTGAATTTATTTTTGGGCTCAAAAGTGATGGCACAGCAAATATAGATATTGAAGATGGAAGTCTGACCATAAA
TTCAAAAGGATCAGGATGGTTCACAATTGTTAATGACCCATGGAGACCTACTCCATCTGACGGTGGTCATTTAGGTCCAA
ATCCAGGAAAGTTTAATAGAAATATTATTGATAAACGCTTTGATGTAGGTGTATTTCAAACCAATTCTTTTGAAGAAGAT
CAATATTTAACAGGAGTCCCCACATTAGAAATTCCAGTAAAAAGTGATCAGCCCAATTTCGATATCTGCCTTGCTTTATC
TCTAGTTAAAAAGGGTGATGAGAAGGTGAATCAATTTTCAACTGGATTCTTAAGGATTAAAAACTCCAAAATAAGTGAAG
AATGCATTTATAAAATAACAATGCAGCCAACAAATATTTGTTTAATTAAAGGTAGCAAGCTTCGCTTATCTATATCTGCA
GCAGCTTATCCCGCTATTGGAGTTAATTCTGGATTTGGGGAGGATAATATTGGGGCGCCTTCAGCAAATCATAGAATTAT
TACTCTAAGTTTTAGCCTTAATAAAACATTTATGAAAATGAACCCTTTTTTTGATAAATAA

Upstream 100 bases:

>100_bases
GATTATGTATCAAAAAAAAATAAGTTGACTCCCGTTAACGCAGAGATCTTTTCTATTTACGTCATTGGGCTTCACTGCCC
AGACATTATCTAAAGCCTAA

Downstream 100 bases:

>100_bases
TTATTTTTTTGGTTAATCATTTGATATTATTAATCCTTAATATCTACCAGTCATGATCGAGACAATTCAAGCAGACTGGA
TTCAATCAGAAGCTATCAAC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MSGSRWFDKSIVLRDGVRLISRIWLPNSNGPWPALLMRQPYGREIASTITYSHPEWWASKGYMVIIQDVRGMGSSEGVFN
GFSQEASDTSETHKWVRSLKECNGKLGLYGFSYQGFTQLTGELNTKPPDCLSPAMTGMNIKDHWCSDGGAYWWHNNIAWG
LQIAALKMKRENNLIEWEKIRIALENKSYLREGIDILKRYDPNSFVLKWLINLNNASPFEEFNPISTWIKQPMLIIGGLW
DPHLKGAFDLYKKSKEAGGSPEIIIGNATHLNWWEGSQESLLKFFDKHLKSDEKFSSKNSQGEKKIWNISLNKWEELDNK
FHPEFIFGLKSDGTANIDIEDGSLTINSKGSGWFTIVNDPWRPTPSDGGHLGPNPGKFNRNIIDKRFDVGVFQTNSFEED
QYLTGVPTLEIPVKSDQPNFDICLALSLVKKGDEKVNQFSTGFLRIKNSKISEECIYKITMQPTNICLIKGSKLRLSISA
AAYPAIGVNSGFGEDNIGAPSANHRIITLSFSLNKTFMKMNPFFDK

Sequences:

>Translated_526_residues
MSGSRWFDKSIVLRDGVRLISRIWLPNSNGPWPALLMRQPYGREIASTITYSHPEWWASKGYMVIIQDVRGMGSSEGVFN
GFSQEASDTSETHKWVRSLKECNGKLGLYGFSYQGFTQLTGELNTKPPDCLSPAMTGMNIKDHWCSDGGAYWWHNNIAWG
LQIAALKMKRENNLIEWEKIRIALENKSYLREGIDILKRYDPNSFVLKWLINLNNASPFEEFNPISTWIKQPMLIIGGLW
DPHLKGAFDLYKKSKEAGGSPEIIIGNATHLNWWEGSQESLLKFFDKHLKSDEKFSSKNSQGEKKIWNISLNKWEELDNK
FHPEFIFGLKSDGTANIDIEDGSLTINSKGSGWFTIVNDPWRPTPSDGGHLGPNPGKFNRNIIDKRFDVGVFQTNSFEED
QYLTGVPTLEIPVKSDQPNFDICLALSLVKKGDEKVNQFSTGFLRIKNSKISEECIYKITMQPTNICLIKGSKLRLSISA
AAYPAIGVNSGFGEDNIGAPSANHRIITLSFSLNKTFMKMNPFFDK
>Mature_525_residues
SGSRWFDKSIVLRDGVRLISRIWLPNSNGPWPALLMRQPYGREIASTITYSHPEWWASKGYMVIIQDVRGMGSSEGVFNG
FSQEASDTSETHKWVRSLKECNGKLGLYGFSYQGFTQLTGELNTKPPDCLSPAMTGMNIKDHWCSDGGAYWWHNNIAWGL
QIAALKMKRENNLIEWEKIRIALENKSYLREGIDILKRYDPNSFVLKWLINLNNASPFEEFNPISTWIKQPMLIIGGLWD
PHLKGAFDLYKKSKEAGGSPEIIIGNATHLNWWEGSQESLLKFFDKHLKSDEKFSSKNSQGEKKIWNISLNKWEELDNKF
HPEFIFGLKSDGTANIDIEDGSLTINSKGSGWFTIVNDPWRPTPSDGGHLGPNPGKFNRNIIDKRFDVGVFQTNSFEEDQ
YLTGVPTLEIPVKSDQPNFDICLALSLVKKGDEKVNQFSTGFLRIKNSKISEECIYKITMQPTNICLIKGSKLRLSISAA
AYPAIGVNSGFGEDNIGAPSANHRIITLSFSLNKTFMKMNPFFDK

Specific function: Hydrolyzes cocaine to benzoate and ecgonine methyl ester, endowing the bacteria with the ability to utilize cocaine as a sole source of carbon and energy for growth, as this bacterium lives in the rhizosphere of coca plants [H]

COG id: COG2936

COG function: function code R; Predicted acyl esterases

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha/beta hydrolase family. Carboxylesterase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005674
- InterPro:   IPR008979
- InterPro:   IPR013736
- InterPro:   IPR000383 [H]

Pfam domain/function: PF02129 Peptidase_S15; PF08530 PepX_C [H]

EC number: NA

Molecular weight: Translated: 59533; Mature: 59402

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGSRWFDKSIVLRDGVRLISRIWLPNSNGPWPALLMRQPYGREIASTITYSHPEWWASK
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHEECCCCHHHHCC
GYMVIIQDVRGMGSSEGVFNGFSQEASDTSETHKWVRSLKECNGKLGLYGFSYQGFTQLT
CEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHC
GELNTKPPDCLSPAMTGMNIKDHWCSDGGAYWWHNNIAWGLQIAALKMKRENNLIEWEKI
CCCCCCCCHHHCHHHCCCCCCCCCCCCCCEEEEECCEEEEEEEEEEEECCCCCCEEEEEE
RIALENKSYLREGIDILKRYDPNSFVLKWLINLNNASPFEEFNPISTWIKQPMLIIGGLW
EEEECCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHCCHHHHHHCCCEEEEECCC
DPHLKGAFDLYKKSKEAGGSPEIIIGNATHLNWWEGSQESLLKFFDKHLKSDEKFSSKNS
CCCCCHHHHHHHHHHHCCCCCCEEECCCEEEECCCCCHHHHHHHHHHHCCCCHHHCCCCC
QGEKKIWNISLNKWEELDNKFHPEFIFGLKSDGTANIDIEDGSLTINSKGSGWFTIVNDP
CCCCEEEEEECHHHHHHCCCCCCEEEEEECCCCEEEEEEECCEEEEECCCCEEEEEECCC
WRPTPSDGGHLGPNPGKFNRNIIDKRFDVGVFQTNSFEEDQYLTGVPTLEIPVKSDQPNF
CCCCCCCCCCCCCCCCCCCCCHHHHHCCCEEEECCCCCCCCEECCCCEEEEEECCCCCCC
DICLALSLVKKGDEKVNQFSTGFLRIKNSKISEECIYKITMQPTNICLIKGSKLRLSISA
EEEEEEHHHHCCHHHHHHHCCCEEEEECCCCCCEEEEEEEECCCEEEEEECCEEEEEEEC
AAYPAIGVNSGFGEDNIGAPSANHRIITLSFSLNKTFMKMNPFFDK
CCCCEEECCCCCCCCCCCCCCCCCEEEEEEEECCCEEEECCCCCCC
>Mature Secondary Structure 
SGSRWFDKSIVLRDGVRLISRIWLPNSNGPWPALLMRQPYGREIASTITYSHPEWWASK
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHEECCCCHHHHCC
GYMVIIQDVRGMGSSEGVFNGFSQEASDTSETHKWVRSLKECNGKLGLYGFSYQGFTQLT
CEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHC
GELNTKPPDCLSPAMTGMNIKDHWCSDGGAYWWHNNIAWGLQIAALKMKRENNLIEWEKI
CCCCCCCCHHHCHHHCCCCCCCCCCCCCCEEEEECCEEEEEEEEEEEECCCCCCEEEEEE
RIALENKSYLREGIDILKRYDPNSFVLKWLINLNNASPFEEFNPISTWIKQPMLIIGGLW
EEEECCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHCCHHHHHHCCCEEEEECCC
DPHLKGAFDLYKKSKEAGGSPEIIIGNATHLNWWEGSQESLLKFFDKHLKSDEKFSSKNS
CCCCCHHHHHHHHHHHCCCCCCEEECCCEEEECCCCCHHHHHHHHHHHCCCCHHHCCCCC
QGEKKIWNISLNKWEELDNKFHPEFIFGLKSDGTANIDIEDGSLTINSKGSGWFTIVNDP
CCCCEEEEEECHHHHHHCCCCCCEEEEEECCCCEEEEEEECCEEEEECCCCEEEEEECCC
WRPTPSDGGHLGPNPGKFNRNIIDKRFDVGVFQTNSFEEDQYLTGVPTLEIPVKSDQPNF
CCCCCCCCCCCCCCCCCCCCCHHHHHCCCEEEECCCCCCCCEECCCCEEEEEECCCCCCC
DICLALSLVKKGDEKVNQFSTGFLRIKNSKISEECIYKITMQPTNICLIKGSKLRLSISA
EEEEEEHHHHCCHHHHHHHCCCEEEEECCCCCCEEEEEEEECCCEEEEEECCEEEEEEEC
AAYPAIGVNSGFGEDNIGAPSANHRIITLSFSLNKTFMKMNPFFDK
CCCCEEECCCCCCCCCCCCCCCCCEEEEEEEECCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10698749; 11742345; 12369817 [H]