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The map label for this gene is gloB [H]

Identifier: 78778944

GI number: 78778944

Start: 521074

End: 521814

Strand: Reverse

Name: gloB [H]

Synonym: PMT9312_0559

Alternate gene names: 78778944

Gene position: 521814-521074 (Counterclockwise)

Preceding gene: 78778948

Following gene: 78778943

Centisome position: 30.53

GC content: 29.55

Gene sequence:

>741_bases
ATGGAATTTAATAAAGCTCAAAATATAATAGGACTTAGAGTTTTAAATGATAATGTCATTTGGTTATGGGAAAAAGATAA
ATCAGTAGTTGTTATAGATCCAGCGATACACGAACCAGTAATTAGATACATAGATGAAAACAATTTGTACATAAAAGCTA
TTTTGCAAACTCATCATCATTCAGATCATATTGGAGGAACGAAATATCTTATTGAAAGATGGCCAAATGTTCAGGTGATT
GCTTCTTCCAAAGATAAAAAGCGAATACCCTTTCAAAATATATCTGTGGAGGATGGTGAAACCTTGAATATCTTAGGCGA
AGAAATAAAAATAATTGAAGTATTAGGACATACAAACTCGCATATCGCCTTCTTTGTAAATGGTGAAAGTCCTGTCCTTT
TTATTGGCGACACTTTATTTTCAGGAGGCTGTGGAAGAATTTTTGAAGGAACATATCAACAAATGTACTCTTCTCTAGAA
AGAATAAAGTCTTTACCAAAAAATACCTTAATATATTGTGCTCATGAATATACCAAGGCAAATCTATTATGGGCATTGAA
TCTCAAACCTAAAGATCAAGATATAAAAAATAAACTTTTGCAAGTTGAAAAAAAACTTTCTATAAACGAATTGACAATTC
CATTTTTACTGGATGAAGAAATGAAAATAAACCTTTTCTTAAGAGCAAAAAATTTAAAAGAATTTACTTTTTTAAGAGAA
AATAAAGACTTATGGGTTTAA

Upstream 100 bases:

>100_bases
TTTTAAAAATTGAAATTGAATCTTCTAACAGAGCTCCTTTTGGTAAAGCTATAGTAAACATAGATCAATTTTTCCAAGGA
TTCTTCATTCTAAGTTAATA

Downstream 100 bases:

>100_bases
ATAGATAAGTATCATCTTAAACAAATGTCCTCTAAGAAAGTAATTAAAACATCTAATGCTCCAGATCCAGTCGGACCTTA
TAATCAAGCAATAAAAGCTG

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MEFNKAQNIIGLRVLNDNVIWLWEKDKSVVVIDPAIHEPVIRYIDENNLYIKAILQTHHHSDHIGGTKYLIERWPNVQVI
ASSKDKKRIPFQNISVEDGETLNILGEEIKIIEVLGHTNSHIAFFVNGESPVLFIGDTLFSGGCGRIFEGTYQQMYSSLE
RIKSLPKNTLIYCAHEYTKANLLWALNLKPKDQDIKNKLLQVEKKLSINELTIPFLLDEEMKINLFLRAKNLKEFTFLRE
NKDLWV

Sequences:

>Translated_246_residues
MEFNKAQNIIGLRVLNDNVIWLWEKDKSVVVIDPAIHEPVIRYIDENNLYIKAILQTHHHSDHIGGTKYLIERWPNVQVI
ASSKDKKRIPFQNISVEDGETLNILGEEIKIIEVLGHTNSHIAFFVNGESPVLFIGDTLFSGGCGRIFEGTYQQMYSSLE
RIKSLPKNTLIYCAHEYTKANLLWALNLKPKDQDIKNKLLQVEKKLSINELTIPFLLDEEMKINLFLRAKNLKEFTFLRE
NKDLWV
>Mature_246_residues
MEFNKAQNIIGLRVLNDNVIWLWEKDKSVVVIDPAIHEPVIRYIDENNLYIKAILQTHHHSDHIGGTKYLIERWPNVQVI
ASSKDKKRIPFQNISVEDGETLNILGEEIKIIEVLGHTNSHIAFFVNGESPVLFIGDTLFSGGCGRIFEGTYQQMYSSLE
RIKSLPKNTLIYCAHEYTKANLLWALNLKPKDQDIKNKLLQVEKKLSINELTIPFLLDEEMKINLFLRAKNLKEFTFLRE
NKDLWV

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI94538322, Length=226, Percent_Identity=34.070796460177, Blast_Score=138, Evalue=6e-33,
Organism=Homo sapiens, GI94538320, Length=226, Percent_Identity=34.070796460177, Blast_Score=137, Evalue=7e-33,
Organism=Homo sapiens, GI14150041, Length=231, Percent_Identity=31.6017316017316, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI21703352, Length=236, Percent_Identity=31.7796610169492, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI116642887, Length=230, Percent_Identity=31.7391304347826, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI46361987, Length=192, Percent_Identity=31.7708333333333, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI41327741, Length=159, Percent_Identity=30.188679245283, Blast_Score=72, Evalue=4e-13,
Organism=Escherichia coli, GI1786406, Length=253, Percent_Identity=39.1304347826087, Blast_Score=176, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17536925, Length=228, Percent_Identity=34.2105263157895, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17538952, Length=209, Percent_Identity=29.1866028708134, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320478, Length=227, Percent_Identity=34.3612334801762, Blast_Score=100, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6324614, Length=226, Percent_Identity=30.5309734513274, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21356335, Length=225, Percent_Identity=34.6666666666667, Blast_Score=142, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24667703, Length=225, Percent_Identity=34.6666666666667, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24667711, Length=225, Percent_Identity=34.6666666666667, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI221330176, Length=158, Percent_Identity=28.4810126582279, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 28515; Mature: 28515

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFNKAQNIIGLRVLNDNVIWLWEKDKSVVVIDPAIHEPVIRYIDENNLYIKAILQTHHH
CCCCCCCCEEEEEEECCCEEEEEECCCEEEEECCCHHCHHHHHCCCCCEEEEEEEECCCC
SDHIGGTKYLIERWPNVQVIASSKDKKRIPFQNISVEDGETLNILGEEIKIIEVLGHTNS
CCCCCCHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEEECCCCC
HIAFFVNGESPVLFIGDTLFSGGCGRIFEGTYQQMYSSLERIKSLPKNTLIYCAHEYTKA
EEEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCE
NLLWALNLKPKDQDIKNKLLQVEKKLSINELTIPFLLDEEMKINLFLRAKNLKEFTFLRE
EEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCEEEEEEEEECCCCEEEEEEC
NKDLWV
CCCCCC
>Mature Secondary Structure
MEFNKAQNIIGLRVLNDNVIWLWEKDKSVVVIDPAIHEPVIRYIDENNLYIKAILQTHHH
CCCCCCCCEEEEEEECCCEEEEEECCCEEEEECCCHHCHHHHHCCCCCEEEEEEEECCCC
SDHIGGTKYLIERWPNVQVIASSKDKKRIPFQNISVEDGETLNILGEEIKIIEVLGHTNS
CCCCCCHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEEECCCCC
HIAFFVNGESPVLFIGDTLFSGGCGRIFEGTYQQMYSSLERIKSLPKNTLIYCAHEYTKA
EEEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCE
NLLWALNLKPKDQDIKNKLLQVEKKLSINELTIPFLLDEEMKINLFLRAKNLKEFTFLRE
EEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCEEEEEEEEECCCCEEEEEEC
NKDLWV
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA