Click here to switch to the map view.

The map label for this gene is mdeA [H]

Identifier: 78778788

GI number: 78778788

Start: 383137

End: 384606

Strand: Reverse

Name: mdeA [H]

Synonym: PMT9312_0403

Alternate gene names: 78778788

Gene position: 384606-383137 (Counterclockwise)

Preceding gene: 78778789

Following gene: 78778787

Centisome position: 22.5

GC content: 29.18

Gene sequence:

>1470_bases
TTGAGAGATTTACTTAAAAACCCTATATGGAAAAATTTAGAGTTAGGATATTCTATTCCTGATAGTATTCATGCCGTTTC
TGTAGCATTACCAACTTGGAATGATGTAATAAATTATGAGGAAAAAAATCAAGAATGCATAAAATTATTGAAGTCCATCT
ACCCACGTTTCGGGCTAAACCCCATAGTGAAAAGATTATGCGAAAAAGTAAAAAAAGAAAATTACTACAATAATAAAAGT
ATCTGGCCATATCCGAATGAAAGCATAGCTTTTAAAGCTAAAAAATACATTGATAGGAATACTTCTGAACAATACTCATT
TATAGAAAAAAGACATAATTTAGCTTTTTTAATAACTGAAAAGGAAGGAAGTATTTATGCAAAATATTTTTGGCAACATA
CTGGTCTTGGTATATCTTCAAGATATGCTGCGGTAGAACTAGGCCTTGAATATAGACCTCCAAAATCTTACGTAAATGAA
TGTTATCAAAGAATAAAAAATAGAATCTCTAAATCTACAAAAATAAACTCTAATGATATTCACTTAACTTCATCTGGAAT
GTCTGCATTGCATACATCATTAGAAATCATATATAAATTATTTCCAGCTAAACCAACACTCCAAATTGGTTTTCCATATG
TAGATGTACTTAAATTACCAATGAATATCTTTTATGGAGCCAAGTTAATTACAGAAGAAAATTGCACCGATATTGAATTA
GAAATCAAAAGAATAAATCCATCAGCATTAATTATTGAACTACCAAGTAATCCAATGCTCAAATGTGTAAACATTAAAAA
AATTTCAAAAATAGCAAAAAGATTAAATATTCCTTTAATCGTTGACGATACAATTGGTTCAAATATAAATATAAATTCCC
TAGAACATGCAGATATAGTTTTTACTTCACTTACAAAAATTTTTTCAGGTAGTGGTGATATTCTTGCTGGATCATTAATA
CTAAATCCAAAAAGCAAATGGATTTATCAGTTTAGAAATGCATTAAAGGAAATTAATCTTCCAATACTTTCAGATGGAGA
TATAGTTTATCTTGAGAAAGTTAGTAGAGATGTAAATCAAAGAGTTTTTGAACAAAATAAAGCATGTTTAGAATTAAAAA
AAAGATTAGAGAACCACAGTGAGATTAAAAATATTTTCCATCCTGAAAATTGTCCAAATTTTAATTCTTTACTTACTTCG
GATGGAGGATACGGCTGCTTATTATCATTTGAATTAAATGGAGGATTGAACAAAGCTAAAAATTTTTATGATTCTCTACA
AATCTCCAAAGGGCCTAGTTTAGGAACAAAATTTACTCTTGTTTGTCCTTATGTTTTACTTGCTCATTATGATGAGTTGG
ATTGGGCTGAAAGTTTTGGTATACCCCCGCACCTTATTAGAGTATCAGTTGGATTAGAGGACCAAGATCAATTATGGGAA
ACCTTTTCTGAAGCACTAAATAATTTCTAA

Upstream 100 bases:

>100_bases
ACTAGGGATAGATGATGCCCTTGTCAGATTATCAATTGGATGTGAAGATGCAAACGATTTAATCTCGGATATTTTATTTG
CTTTAAATAAATTTTGAAAA

Downstream 100 bases:

>100_bases
ATTCCTAGTATTTACCGTATAGAAACTTATATACTCTTTTTCTGAATAATAGTTAGTATTAAAATATATTTTCTCAATAT
ATAAATGGCAAGAATTTATG

Product: putative cystathionine gamma-synthase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MRDLLKNPIWKNLELGYSIPDSIHAVSVALPTWNDVINYEEKNQECIKLLKSIYPRFGLNPIVKRLCEKVKKENYYNNKS
IWPYPNESIAFKAKKYIDRNTSEQYSFIEKRHNLAFLITEKEGSIYAKYFWQHTGLGISSRYAAVELGLEYRPPKSYVNE
CYQRIKNRISKSTKINSNDIHLTSSGMSALHTSLEIIYKLFPAKPTLQIGFPYVDVLKLPMNIFYGAKLITEENCTDIEL
EIKRINPSALIIELPSNPMLKCVNIKKISKIAKRLNIPLIVDDTIGSNININSLEHADIVFTSLTKIFSGSGDILAGSLI
LNPKSKWIYQFRNALKEINLPILSDGDIVYLEKVSRDVNQRVFEQNKACLELKKRLENHSEIKNIFHPENCPNFNSLLTS
DGGYGCLLSFELNGGLNKAKNFYDSLQISKGPSLGTKFTLVCPYVLLAHYDELDWAESFGIPPHLIRVSVGLEDQDQLWE
TFSEALNNF

Sequences:

>Translated_489_residues
MRDLLKNPIWKNLELGYSIPDSIHAVSVALPTWNDVINYEEKNQECIKLLKSIYPRFGLNPIVKRLCEKVKKENYYNNKS
IWPYPNESIAFKAKKYIDRNTSEQYSFIEKRHNLAFLITEKEGSIYAKYFWQHTGLGISSRYAAVELGLEYRPPKSYVNE
CYQRIKNRISKSTKINSNDIHLTSSGMSALHTSLEIIYKLFPAKPTLQIGFPYVDVLKLPMNIFYGAKLITEENCTDIEL
EIKRINPSALIIELPSNPMLKCVNIKKISKIAKRLNIPLIVDDTIGSNININSLEHADIVFTSLTKIFSGSGDILAGSLI
LNPKSKWIYQFRNALKEINLPILSDGDIVYLEKVSRDVNQRVFEQNKACLELKKRLENHSEIKNIFHPENCPNFNSLLTS
DGGYGCLLSFELNGGLNKAKNFYDSLQISKGPSLGTKFTLVCPYVLLAHYDELDWAESFGIPPHLIRVSVGLEDQDQLWE
TFSEALNNF
>Mature_489_residues
MRDLLKNPIWKNLELGYSIPDSIHAVSVALPTWNDVINYEEKNQECIKLLKSIYPRFGLNPIVKRLCEKVKKENYYNNKS
IWPYPNESIAFKAKKYIDRNTSEQYSFIEKRHNLAFLITEKEGSIYAKYFWQHTGLGISSRYAAVELGLEYRPPKSYVNE
CYQRIKNRISKSTKINSNDIHLTSSGMSALHTSLEIIYKLFPAKPTLQIGFPYVDVLKLPMNIFYGAKLITEENCTDIEL
EIKRINPSALIIELPSNPMLKCVNIKKISKIAKRLNIPLIVDDTIGSNININSLEHADIVFTSLTKIFSGSGDILAGSLI
LNPKSKWIYQFRNALKEINLPILSDGDIVYLEKVSRDVNQRVFEQNKACLELKKRLENHSEIKNIFHPENCPNFNSLLTS
DGGYGCLLSFELNGGLNKAKNFYDSLQISKGPSLGTKFTLVCPYVLLAHYDELDWAESFGIPPHLIRVSVGLEDQDQLWE
TFSEALNNF

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1790375, Length=322, Percent_Identity=25.1552795031056, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17560056, Length=273, Percent_Identity=27.8388278388278, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17538055, Length=262, Percent_Identity=27.4809160305344, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6322970, Length=566, Percent_Identity=31.6254416961131, Blast_Score=220, Evalue=4e-58,
Organism=Saccharomyces cerevisiae, GI6322590, Length=354, Percent_Identity=35.3107344632768, Blast_Score=186, Evalue=6e-48,
Organism=Saccharomyces cerevisiae, GI6323557, Length=345, Percent_Identity=34.2028985507246, Blast_Score=178, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6319307, Length=250, Percent_Identity=30.4, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19922554, Length=262, Percent_Identity=27.8625954198473, Blast_Score=85, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 55905; Mature: 55905

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDLLKNPIWKNLELGYSIPDSIHAVSVALPTWNDVINYEEKNQECIKLLKSIYPRFGLN
CCHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHCCCCCCCHHHHHHHHHHCCCCCCC
PIVKRLCEKVKKENYYNNKSIWPYPNESIAFKAKKYIDRNTSEQYSFIEKRHNLAFLITE
HHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEEE
KEGSIYAKYFWQHTGLGISSRYAAVELGLEYRPPKSYVNECYQRIKNRISKSTKINSNDI
CCCCEEEEEEHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCE
HLTSSGMSALHTSLEIIYKLFPAKPTLQIGFPYVDVLKLPMNIFYGAKLITEENCTDIEL
EEECCCHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHCCEEEECCCCCCEEE
EIKRINPSALIIELPSNPMLKCVNIKKISKIAKRLNIPLIVDDTIGSNININSLEHADIV
EEEEECCCEEEEECCCCCCEEEECHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHH
FTSLTKIFSGSGDILAGSLILNPKSKWIYQFRNALKEINLPILSDGDIVYLEKVSRDVNQ
HHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHHHHCCCCEECCCCEEEHHHHHHHHHH
RVFEQNKACLELKKRLENHSEIKNIFHPENCPNFNSLLTSDGGYGCLLSFELNGGLNKAK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCEEEEEEEECCCCHHHH
NFYDSLQISKGPSLGTKFTLVCPYVLLAHYDELDWAESFGIPPHLIRVSVGLEDQDQLWE
HHHHHCEECCCCCCCCCHHHHHHHHHHHHCCCCCHHHHCCCCCEEEEEEECCCCHHHHHH
TFSEALNNF
HHHHHHHCC
>Mature Secondary Structure
MRDLLKNPIWKNLELGYSIPDSIHAVSVALPTWNDVINYEEKNQECIKLLKSIYPRFGLN
CCHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHCCCCCCCHHHHHHHHHHCCCCCCC
PIVKRLCEKVKKENYYNNKSIWPYPNESIAFKAKKYIDRNTSEQYSFIEKRHNLAFLITE
HHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEEE
KEGSIYAKYFWQHTGLGISSRYAAVELGLEYRPPKSYVNECYQRIKNRISKSTKINSNDI
CCCCEEEEEEHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCE
HLTSSGMSALHTSLEIIYKLFPAKPTLQIGFPYVDVLKLPMNIFYGAKLITEENCTDIEL
EEECCCHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHCCEEEECCCCCCEEE
EIKRINPSALIIELPSNPMLKCVNIKKISKIAKRLNIPLIVDDTIGSNININSLEHADIV
EEEEECCCEEEEECCCCCCEEEECHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHH
FTSLTKIFSGSGDILAGSLILNPKSKWIYQFRNALKEINLPILSDGDIVYLEKVSRDVNQ
HHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHHHHCCCCEECCCCEEEHHHHHHHHHH
RVFEQNKACLELKKRLENHSEIKNIFHPENCPNFNSLLTSDGGYGCLLSFELNGGLNKAK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCEEEEEEEECCCCHHHH
NFYDSLQISKGPSLGTKFTLVCPYVLLAHYDELDWAESFGIPPHLIRVSVGLEDQDQLWE
HHHHHCEECCCCCCCCCHHHHHHHHHHHHCCCCCHHHHCCCCCEEEEEEECCCCHHHHHH
TFSEALNNF
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]