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The map label for this gene is tgt

Identifier: 78778658

GI number: 78778658

Start: 261241

End: 262359

Strand: Direct

Name: tgt

Synonym: PMT9312_0273

Alternate gene names: 78778658

Gene position: 261241-262359 (Clockwise)

Preceding gene: 78778656

Following gene: 78778659

Centisome position: 15.28

GC content: 34.41

Gene sequence:

>1119_bases
GTGTTTCAATTTGAAATTACATCGAATTGCAGTAATACAGATGCAAGAACTGGTATATTTCATACCCCAAATGGTCAGGT
AAACACTCCAAGATTTATGCCTGTGGGTACTTTGGGAACGGTTAAAGGAATTTCATCTAAGCAGTTAACCTCTACAGGAT
CAGAAATGATTCTCTCAAATACCTTCCATCTTCATTTGCAACCTGGAGAAAAACTAGTTAAAGAATCTGGTGGAATACAT
AAGTTCATGAATTGGCCTAAGCCTATTCTTACTGATTCAGGTGGATATCAAGTTTTTAGTTTGGCTAAATTAAATAATAT
TTCTGATAAAGGTGTGGAATTTAAAAATCCAAGAGATGGTAGTCATGTATTTTTATCACCCGAAAAAGTAATGCAGATTC
AAATGGATCTTGGCTCGGATGTTGCGATGGCTTTTGATCATTGCCCTCCGCATACAGCTAATGAAAATGATATAGAGGAC
TCCTTACTAAGAACTCATTCTTGGTTGCAAAAATGTGTTGAGACTCATCAGAAATCAAATCAAGCATTATTCGGAATAAT
TCAAGGTGGTAAGTATCCTAGATTAAGAGAATATAGCGCAAAATTTACAAGTTCTTTTGATCTGCCTGGAATAGCGGTAG
GGGGTGTCAGTGTTGGAGAGGCAGTCGAAGAAATACATAGTGTAATTAATTACATCCCGAAATTCTTACCAATAAATAAA
CCAAGATATTTAATGGGAATTGGCTCTTTAAGAGAAATTTCTTTAGCTGTTGCTAAAGGATTCGATATATTTGACTGTGT
TTTGCCTACAAGACTAGGAAGACATGGGACTGCATTTTTTAATGATCAAAGATTGAATTTGCGAAATGCTCGATTTAAAA
ATGATTTTTCTCCGATTGATAAAACTTGTAAATGCGAAACCTGTAAGTCCTATTCTCGTGCATATTTGCATCATTTAATT
AGAAATGATGAAATATTAGGTCTCACCTTAATAAGTTTGCATAATATTGCTCATTTAATAAGATTTACCAATGCCATTTC
TAATGCAATTAAAGATAATTGTTTTACAATTGATTTCGCTCCTTGGAAAACAGCCTCTATTGCTCACCATACGTGGTAA

Upstream 100 bases:

>100_bases
AAATTTTTTATTAAATATTTTTTGATGATATGAAAATAGATTCAATATTAAATAATAGGGTAGATTAATAAAAAAGGATT
AAAAATTTTTATAAATTATC

Downstream 100 bases:

>100_bases
CGTCTTATCATAATTAATTAAATTATTAAAAAAGGTGCTCATTCTATTTAATACATTCGCTGAATTGCCTGAGGCTTACA
AGGCTTTTGCTCCAACTGTT

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MFQFEITSNCSNTDARTGIFHTPNGQVNTPRFMPVGTLGTVKGISSKQLTSTGSEMILSNTFHLHLQPGEKLVKESGGIH
KFMNWPKPILTDSGGYQVFSLAKLNNISDKGVEFKNPRDGSHVFLSPEKVMQIQMDLGSDVAMAFDHCPPHTANENDIED
SLLRTHSWLQKCVETHQKSNQALFGIIQGGKYPRLREYSAKFTSSFDLPGIAVGGVSVGEAVEEIHSVINYIPKFLPINK
PRYLMGIGSLREISLAVAKGFDIFDCVLPTRLGRHGTAFFNDQRLNLRNARFKNDFSPIDKTCKCETCKSYSRAYLHHLI
RNDEILGLTLISLHNIAHLIRFTNAISNAIKDNCFTIDFAPWKTASIAHHTW

Sequences:

>Translated_372_residues
MFQFEITSNCSNTDARTGIFHTPNGQVNTPRFMPVGTLGTVKGISSKQLTSTGSEMILSNTFHLHLQPGEKLVKESGGIH
KFMNWPKPILTDSGGYQVFSLAKLNNISDKGVEFKNPRDGSHVFLSPEKVMQIQMDLGSDVAMAFDHCPPHTANENDIED
SLLRTHSWLQKCVETHQKSNQALFGIIQGGKYPRLREYSAKFTSSFDLPGIAVGGVSVGEAVEEIHSVINYIPKFLPINK
PRYLMGIGSLREISLAVAKGFDIFDCVLPTRLGRHGTAFFNDQRLNLRNARFKNDFSPIDKTCKCETCKSYSRAYLHHLI
RNDEILGLTLISLHNIAHLIRFTNAISNAIKDNCFTIDFAPWKTASIAHHTW
>Mature_372_residues
MFQFEITSNCSNTDARTGIFHTPNGQVNTPRFMPVGTLGTVKGISSKQLTSTGSEMILSNTFHLHLQPGEKLVKESGGIH
KFMNWPKPILTDSGGYQVFSLAKLNNISDKGVEFKNPRDGSHVFLSPEKVMQIQMDLGSDVAMAFDHCPPHTANENDIED
SLLRTHSWLQKCVETHQKSNQALFGIIQGGKYPRLREYSAKFTSSFDLPGIAVGGVSVGEAVEEIHSVINYIPKFLPINK
PRYLMGIGSLREISLAVAKGFDIFDCVLPTRLGRHGTAFFNDQRLNLRNARFKNDFSPIDKTCKCETCKSYSRAYLHHLI
RNDEILGLTLISLHNIAHLIRFTNAISNAIKDNCFTIDFAPWKTASIAHHTW

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=353, Percent_Identity=41.9263456090652, Blast_Score=315, Evalue=5e-86,
Organism=Homo sapiens, GI13375872, Length=334, Percent_Identity=22.7544910179641, Blast_Score=90, Evalue=3e-18,
Organism=Escherichia coli, GI1786607, Length=363, Percent_Identity=43.801652892562, Blast_Score=327, Evalue=8e-91,
Organism=Caenorhabditis elegans, GI17544680, Length=340, Percent_Identity=42.3529411764706, Blast_Score=285, Evalue=3e-77,
Organism=Caenorhabditis elegans, GI25146502, Length=223, Percent_Identity=23.3183856502242, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI19920510, Length=353, Percent_Identity=40.7932011331445, Blast_Score=310, Evalue=7e-85,
Organism=Drosophila melanogaster, GI21355311, Length=252, Percent_Identity=25.3968253968254, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 41580; Mature: 41580

Theoretical pI: Translated: 8.57; Mature: 8.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQFEITSNCSNTDARTGIFHTPNGQVNTPRFMPVGTLGTVKGISSKQLTSTGSEMILSN
CEEEEEECCCCCCCCCCCEEECCCCCCCCCCEEECCCCHHHCCCCCHHHHCCCCCEEEEC
TFHLHLQPGEKLVKESGGIHKFMNWPKPILTDSGGYQVFSLAKLNNISDKGVEFKNPRDG
EEEEEECCHHHHHHHCCCCHHHHCCCCCCEECCCCCEEEEEEECCCCCCCCCCCCCCCCC
SHVFLSPEKVMQIQMDLGSDVAMAFDHCPPHTANENDIEDSLLRTHSWLQKCVETHQKSN
CEEEECHHHHEEEEECCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
QALFGIIQGGKYPRLREYSAKFTSSFDLPGIAVGGVSVGEAVEEIHSVINYIPKFLPINK
CEEEEEECCCCCCCHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCC
PRYLMGIGSLREISLAVAKGFDIFDCVLPTRLGRHGTAFFNDQRLNLRNARFKNDFSPID
CCEEEECCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCC
KTCKCETCKSYSRAYLHHLIRNDEILGLTLISLHNIAHLIRFTNAISNAIKDNCFTIDFA
CCCCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECC
PWKTASIAHHTW
CCCCCCCCCCCC
>Mature Secondary Structure
MFQFEITSNCSNTDARTGIFHTPNGQVNTPRFMPVGTLGTVKGISSKQLTSTGSEMILSN
CEEEEEECCCCCCCCCCCEEECCCCCCCCCCEEECCCCHHHCCCCCHHHHCCCCCEEEEC
TFHLHLQPGEKLVKESGGIHKFMNWPKPILTDSGGYQVFSLAKLNNISDKGVEFKNPRDG
EEEEEECCHHHHHHHCCCCHHHHCCCCCCEECCCCCEEEEEEECCCCCCCCCCCCCCCCC
SHVFLSPEKVMQIQMDLGSDVAMAFDHCPPHTANENDIEDSLLRTHSWLQKCVETHQKSN
CEEEECHHHHEEEEECCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
QALFGIIQGGKYPRLREYSAKFTSSFDLPGIAVGGVSVGEAVEEIHSVINYIPKFLPINK
CEEEEEECCCCCCCHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCC
PRYLMGIGSLREISLAVAKGFDIFDCVLPTRLGRHGTAFFNDQRLNLRNARFKNDFSPID
CCEEEECCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCC
KTCKCETCKSYSRAYLHHLIRNDEILGLTLISLHNIAHLIRFTNAISNAIKDNCFTIDFA
CCCCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECC
PWKTASIAHHTW
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA