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The map label for this gene is cobS [H]

Identifier: 78778657

GI number: 78778657

Start: 260402

End: 261142

Strand: Reverse

Name: cobS [H]

Synonym: PMT9312_0272

Alternate gene names: 78778657

Gene position: 261142-260402 (Counterclockwise)

Preceding gene: 78778660

Following gene: 78778655

Centisome position: 15.28

GC content: 29.28

Gene sequence:

>741_bases
TTGGCAGGATCTTGGATTTTCTATACAACCTTTCCAAAGATACCTTTTATTAATCCCGAATTTAAAAATATTGCACAATT
TGCGCCGCCTTTGGGATTTTTTATTGGAACAATACAGAGTTATATTTTTATTTTTTTAACAGCAAACTCTTGGTCTATCT
ATGCATCTACATTAATTTGTTTGGCTTCAGGATATTTAATTACTGGCGGTCTACACCTGGATGGTTTAATGGATACTTTC
GATGGTATTTTTGCGGGCAAAAAGAAACGTTTAAAAGCTATGAAAGACAGTAAAGTTGGGTCCTTTGGCGTTCAGTCGTT
AGTTTTTATAACTTTAATTCAGATTGCTTGCATACTCAAAATTCAAAACCAAATAATTATTGTTTTACCAATATGCTTAT
TTTGGGGGAGATTTTCAAATTTATTTTTTATAGAAAAATTTAAATATATGAGTTTCAAGAAAAAATCTATTAGTCATAAA
AAGTTTTGGAATGGATTTAAAAAAGAATCTTTGATTTCCATTATTTTTCTTTTAATTTTTATTGCATACCAATTAGTGTC
AATTACAACCCAAGCAATATTAATTAAATTTTTATTTCTTATTTTGATTGGTACTTTCTTAAGTTATTCTATTCCAAACA
TGCTGGGTAATAAAATTGGAGGCTTCAATGGAGATGCCTGTGGTGCGAGTGTTGTACTTGTTGAAACTGCAATGTTATTT
ATGCATTCAATTCTTTTATAG

Upstream 100 bases:

>100_bases
ACGATAATTTATAAAAATTTTTAATCCTTTTTTATTAATCTACCCTATTATTTAATATTGAATCTATTTTCATATCATCA
AAAAATATTTAATAAAAAAT

Downstream 100 bases:

>100_bases
GAAGCTCTAAGTAGAAAATATTTTTCTTCTTAAGATTTTTTAATTCCTCAGTATTATCAATTGAGTAATTTTCCAGCAAT
CTCAACTCTCCTCCAATTTG

Product: cobalamin-5'-phosphate synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MAGSWIFYTTFPKIPFINPEFKNIAQFAPPLGFFIGTIQSYIFIFLTANSWSIYASTLICLASGYLITGGLHLDGLMDTF
DGIFAGKKKRLKAMKDSKVGSFGVQSLVFITLIQIACILKIQNQIIIVLPICLFWGRFSNLFFIEKFKYMSFKKKSISHK
KFWNGFKKESLISIIFLLIFIAYQLVSITTQAILIKFLFLILIGTFLSYSIPNMLGNKIGGFNGDACGASVVLVETAMLF
MHSILL

Sequences:

>Translated_246_residues
MAGSWIFYTTFPKIPFINPEFKNIAQFAPPLGFFIGTIQSYIFIFLTANSWSIYASTLICLASGYLITGGLHLDGLMDTF
DGIFAGKKKRLKAMKDSKVGSFGVQSLVFITLIQIACILKIQNQIIIVLPICLFWGRFSNLFFIEKFKYMSFKKKSISHK
KFWNGFKKESLISIIFLLIFIAYQLVSITTQAILIKFLFLILIGTFLSYSIPNMLGNKIGGFNGDACGASVVLVETAMLF
MHSILL
>Mature_245_residues
AGSWIFYTTFPKIPFINPEFKNIAQFAPPLGFFIGTIQSYIFIFLTANSWSIYASTLICLASGYLITGGLHLDGLMDTFD
GIFAGKKKRLKAMKDSKVGSFGVQSLVFITLIQIACILKIQNQIIIVLPICLFWGRFSNLFFIEKFKYMSFKKKSISHKK
FWNGFKKESLISIIFLLIFIAYQLVSITTQAILIKFLFLILIGTFLSYSIPNMLGNKIGGFNGDACGASVVLVETAMLFM
HSILL

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=212, Percent_Identity=25, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 27651; Mature: 27519

Theoretical pI: Translated: 10.22; Mature: 10.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGSWIFYTTFPKIPFINPEFKNIAQFAPPLGFFIGTIQSYIFIFLTANSWSIYASTLIC
CCCCEEEEEECCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHEEEEECCCCHHHHHHHHH
LASGYLITGGLHLDGLMDTFDGIFAGKKKRLKAMKDSKVGSFGVQSLVFITLIQIACILK
HHCCCCEECCCCHHHHHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
IQNQIIIVLPICLFWGRFSNLFFIEKFKYMSFKKKSISHKKFWNGFKKESLISIIFLLIF
HCCCEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHH
IAYQLVSITTQAILIKFLFLILIGTFLSYSIPNMLGNKIGGFNGDACGASVVLVETAMLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
MHSILL
HHHHHC
>Mature Secondary Structure 
AGSWIFYTTFPKIPFINPEFKNIAQFAPPLGFFIGTIQSYIFIFLTANSWSIYASTLIC
CCCEEEEEECCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHEEEEECCCCHHHHHHHHH
LASGYLITGGLHLDGLMDTFDGIFAGKKKRLKAMKDSKVGSFGVQSLVFITLIQIACILK
HHCCCCEECCCCHHHHHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
IQNQIIIVLPICLFWGRFSNLFFIEKFKYMSFKKKSISHKKFWNGFKKESLISIIFLLIF
HCCCEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHH
IAYQLVSITTQAILIKFLFLILIGTFLSYSIPNMLGNKIGGFNGDACGASVVLVETAMLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
MHSILL
HHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12917486 [H]