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The map label for this gene is purH

Identifier: 78778653

GI number: 78778653

Start: 256469

End: 258022

Strand: Reverse

Name: purH

Synonym: PMT9312_0268

Alternate gene names: 78778653

Gene position: 258022-256469 (Counterclockwise)

Preceding gene: 78778655

Following gene: 78778648

Centisome position: 15.1

GC content: 32.95

Gene sequence:

>1554_bases
ATGTCACCATTAGCTTTAGTAAGTGTCTCAGATAAAAAACATATAGTCCCATTTTGTATGGAATTGGTAGAACAATTTAA
TTATAGAATTCTATCAAGTGGAGGAACTGCCAAACATCTAATAGAGGCAAATATTCCAGTTATTAAAGTTGCAGATTTTA
CTAATTCTCCAGAAATCCTTGGAGGAAGAGTGAAAACTTTACATCCAAAAATACACGGAGGAATATTAGCTAAAAGAACT
GATGAAGAACATAAAAGAGATATAGAAGCTAACGATCTTGAGTTAATTGACTTAGTAGTTGTCAATTTATATCCTTTTAA
GAAAACTGTAGAACAGGGATCTAAATGGGAAGATTCTATTGAAAATATCGATATAGGAGGGCCATCAATGATTCGTTCTG
CAGCTAAAAATCATAAAGACGTTTCTGTTTTAGTCGATCCTAGTCAATATCAGAATTTTCTTGAAGAAAGTAAAAAAGGT
GAGTTAAAGGATTCATATAAAGCACAATTAGCCCTTGAAGCTTTTCAACATACAGCAGACTATGACACTGCAATATCTAA
TTGGATAAGTAAAGAAAGAGGTTTACAATCCTCTAAATATATTGAATCTTATCCACTAATCAACACCTTAAGATATGGGG
AGAATCCTCATCAAAAAGCTTTGTGGTATGGTTTGAGTAATATTGGATGGAACTCAGCAGAACAATTACAAGGTAAAGAT
TTAAGTTATAACAATTTATTAGATCTTGAGTCAGCACTTTCAACAGTTTTAGAATTTGGCTATGCAGAAAAAGATGAACT
TACTACAGATACGTTTGCATCTGTTATTCTAAAACACAATAATCCTTGTGGTGCCTCTATAAGTAATTCAGCCTCTCAAG
CATTTTTGAATGCTTTGGAATGCGACTCAGTTAGTGCATTTGGAGGAATAGTTGCTTTTAATTCAAATGTTGATAGTGAA
ACCGCAATTAACCTCAAAGATATTTTCCTAGAGTGTGTCGTAGCTCCATCTTTTGATGCGGAAGCTTTAGAAATTTTAAA
AATCAAAAAGAATTTAAGAATTTTAAAGTTATCAAAAGATCAACTCCCAAAAAAGAAGCAAACTTCTACTAAATCAATAA
TGGGAGGATTACTTGTTCAAGACACTAATGATAGTGAAGATAAAACTGAAAGTTGGATGTCAGTAACTAAGAATAATCCG
AGTAATCAAATGAACTTAGATCTAAATTTTGCATGGAAAATTTGTAAACATGTGAAATCGAATGCGATTGTTATTGCAAA
AGACCAAAAAACTATTGGTATAGGAGCTGGACAAATGAATAGAGTTGGAGCAGCAAAAATCGCATTACAAGCAGCTGGAA
AGTTATGTTCTGATGCTGTCTTGGCCAGTGATGGGTTTTTTCCATTTGCAGATACTGTAGAACTTGCAAATGAGTATGGT
ATAAAAGCAATTATCCAACCTGGAGGGAGTCTAAGAGACCAAGAAAGTATTGATATGTGTAATTCTAAAGGAATCTCAAT
GGTAATTACGCAAAAAAGGCATTTTTTGCATTAG

Upstream 100 bases:

>100_bases
AAATAATTCTATGAGTTGCAGTTTGAGAGCTAATCGAGACAAATTCATGATGGATATCGTATTTCATGTTTATATTCTTA
AGTAAATAAACTTTCTCATA

Downstream 100 bases:

>100_bases
GTTAGTTATGTTGATTTTGGATAATATGTAATCTTGTTAGTCTTTCTGAATAAAGAGAGCCTGCCTGGACCTGCGCATAG
AAAATAGAGAGAAATAGCAC

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 517; Mature: 516

Protein sequence:

>517_residues
MSPLALVSVSDKKHIVPFCMELVEQFNYRILSSGGTAKHLIEANIPVIKVADFTNSPEILGGRVKTLHPKIHGGILAKRT
DEEHKRDIEANDLELIDLVVVNLYPFKKTVEQGSKWEDSIENIDIGGPSMIRSAAKNHKDVSVLVDPSQYQNFLEESKKG
ELKDSYKAQLALEAFQHTADYDTAISNWISKERGLQSSKYIESYPLINTLRYGENPHQKALWYGLSNIGWNSAEQLQGKD
LSYNNLLDLESALSTVLEFGYAEKDELTTDTFASVILKHNNPCGASISNSASQAFLNALECDSVSAFGGIVAFNSNVDSE
TAINLKDIFLECVVAPSFDAEALEILKIKKNLRILKLSKDQLPKKKQTSTKSIMGGLLVQDTNDSEDKTESWMSVTKNNP
SNQMNLDLNFAWKICKHVKSNAIVIAKDQKTIGIGAGQMNRVGAAKIALQAAGKLCSDAVLASDGFFPFADTVELANEYG
IKAIIQPGGSLRDQESIDMCNSKGISMVITQKRHFLH

Sequences:

>Translated_517_residues
MSPLALVSVSDKKHIVPFCMELVEQFNYRILSSGGTAKHLIEANIPVIKVADFTNSPEILGGRVKTLHPKIHGGILAKRT
DEEHKRDIEANDLELIDLVVVNLYPFKKTVEQGSKWEDSIENIDIGGPSMIRSAAKNHKDVSVLVDPSQYQNFLEESKKG
ELKDSYKAQLALEAFQHTADYDTAISNWISKERGLQSSKYIESYPLINTLRYGENPHQKALWYGLSNIGWNSAEQLQGKD
LSYNNLLDLESALSTVLEFGYAEKDELTTDTFASVILKHNNPCGASISNSASQAFLNALECDSVSAFGGIVAFNSNVDSE
TAINLKDIFLECVVAPSFDAEALEILKIKKNLRILKLSKDQLPKKKQTSTKSIMGGLLVQDTNDSEDKTESWMSVTKNNP
SNQMNLDLNFAWKICKHVKSNAIVIAKDQKTIGIGAGQMNRVGAAKIALQAAGKLCSDAVLASDGFFPFADTVELANEYG
IKAIIQPGGSLRDQESIDMCNSKGISMVITQKRHFLH
>Mature_516_residues
SPLALVSVSDKKHIVPFCMELVEQFNYRILSSGGTAKHLIEANIPVIKVADFTNSPEILGGRVKTLHPKIHGGILAKRTD
EEHKRDIEANDLELIDLVVVNLYPFKKTVEQGSKWEDSIENIDIGGPSMIRSAAKNHKDVSVLVDPSQYQNFLEESKKGE
LKDSYKAQLALEAFQHTADYDTAISNWISKERGLQSSKYIESYPLINTLRYGENPHQKALWYGLSNIGWNSAEQLQGKDL
SYNNLLDLESALSTVLEFGYAEKDELTTDTFASVILKHNNPCGASISNSASQAFLNALECDSVSAFGGIVAFNSNVDSET
AINLKDIFLECVVAPSFDAEALEILKIKKNLRILKLSKDQLPKKKQTSTKSIMGGLLVQDTNDSEDKTESWMSVTKNNPS
NQMNLDLNFAWKICKHVKSNAIVIAKDQKTIGIGAGQMNRVGAAKIALQAAGKLCSDAVLASDGFFPFADTVELANEYGI
KAIIQPGGSLRDQESIDMCNSKGISMVITQKRHFLH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=478, Percent_Identity=37.2384937238494, Blast_Score=233, Evalue=3e-61,
Organism=Escherichia coli, GI1790439, Length=537, Percent_Identity=45.2513966480447, Blast_Score=412, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI71985564, Length=474, Percent_Identity=35.0210970464135, Blast_Score=239, Evalue=3e-63,
Organism=Caenorhabditis elegans, GI71985574, Length=304, Percent_Identity=27.3026315789474, Blast_Score=86, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6323056, Length=473, Percent_Identity=36.1522198731501, Blast_Score=252, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6323768, Length=469, Percent_Identity=36.0341151385927, Blast_Score=252, Evalue=1e-67,
Organism=Drosophila melanogaster, GI24649832, Length=477, Percent_Identity=34.8008385744235, Blast_Score=225, Evalue=7e-59,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 56960; Mature: 56829

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPLALVSVSDKKHIVPFCMELVEQFNYRILSSGGTAKHLIEANIPVIKVADFTNSPEIL
CCCEEEEEECCCCCHHHHHHHHHHHCCEEEEECCCCCHHEEECCCCEEEEECCCCCCHHH
GGRVKTLHPKIHGGILAKRTDEEHKRDIEANDLELIDLVVVNLYPFKKTVEQGSKWEDSI
CCCEEEECCHHHCCEEECCCCHHHHCCCCCCCCEEEEEEEHHCCCHHHHHHCCCCHHHHH
ENIDIGGPSMIRSAAKNHKDVSVLVDPSQYQNFLEESKKGELKDSYKAQLALEAFQHTAD
CEECCCCHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCC
YDTAISNWISKERGLQSSKYIESYPLINTLRYGENPHQKALWYGLSNIGWNSAEQLQGKD
HHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHCCCC
LSYNNLLDLESALSTVLEFGYAEKDELTTDTFASVILKHNNPCGASISNSASQAFLNALE
CCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHC
CDSVSAFGGIVAFNSNVDSETAINLKDIFLECVVAPSFDAEALEILKIKKNLRILKLSKD
CCCCHHCCCEEEECCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEECHH
QLPKKKQTSTKSIMGGLLVQDTNDSEDKTESWMSVTKNNPSNQMNLDLNFAWKICKHVKS
HCCCHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHCC
NAIVIAKDQKTIGIGAGQMNRVGAAKIALQAAGKLCSDAVLASDGFFPFADTVELANEYG
CEEEEEECCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHCC
IKAIIQPGGSLRDQESIDMCNSKGISMVITQKRHFLH
CEEEECCCCCCCCCHHHHHHCCCCCEEEEECCHHCCC
>Mature Secondary Structure 
SPLALVSVSDKKHIVPFCMELVEQFNYRILSSGGTAKHLIEANIPVIKVADFTNSPEIL
CCEEEEEECCCCCHHHHHHHHHHHCCEEEEECCCCCHHEEECCCCEEEEECCCCCCHHH
GGRVKTLHPKIHGGILAKRTDEEHKRDIEANDLELIDLVVVNLYPFKKTVEQGSKWEDSI
CCCEEEECCHHHCCEEECCCCHHHHCCCCCCCCEEEEEEEHHCCCHHHHHHCCCCHHHHH
ENIDIGGPSMIRSAAKNHKDVSVLVDPSQYQNFLEESKKGELKDSYKAQLALEAFQHTAD
CEECCCCHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCC
YDTAISNWISKERGLQSSKYIESYPLINTLRYGENPHQKALWYGLSNIGWNSAEQLQGKD
HHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHCCCC
LSYNNLLDLESALSTVLEFGYAEKDELTTDTFASVILKHNNPCGASISNSASQAFLNALE
CCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHC
CDSVSAFGGIVAFNSNVDSETAINLKDIFLECVVAPSFDAEALEILKIKKNLRILKLSKD
CCCCHHCCCEEEECCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEECHH
QLPKKKQTSTKSIMGGLLVQDTNDSEDKTESWMSVTKNNPSNQMNLDLNFAWKICKHVKS
HCCCHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHCC
NAIVIAKDQKTIGIGAGQMNRVGAAKIALQAAGKLCSDAVLASDGFFPFADTVELANEYG
CEEEEEECCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHCC
IKAIIQPGGSLRDQESIDMCNSKGISMVITQKRHFLH
CEEEECCCCCCCCCHHHHHHCCCCCEEEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA