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The map label for this gene is amtB [C]

Identifier: 78778650

GI number: 78778650

Start: 253049

End: 254512

Strand: Direct

Name: amtB [C]

Synonym: PMT9312_0265

Alternate gene names: 78778650

Gene position: 253049-254512 (Clockwise)

Preceding gene: 78778649

Following gene: 78778651

Centisome position: 14.81

GC content: 40.98

Gene sequence:

>1464_bases
ATGACCACTGCTTTGCAAACGCCTCAAAGGCGCTCTAGGACCAGATTACAAGACGCAAGTCTTGTAAATGGACCTATGCT
CCTTTTGAGGAGTATTCGAGGATTTAGTTCAAACCGCTCCATGTTGTGGCTTGCAACTGTTCCTTTAGCACTTTTTGGAT
TAGGACTTTTTAATGTTGCTGCTCATGCAACAGAACTTCCTGATCTTAATGCAGCATTTTTAGCAAATAACCTTTGGTTA
TTTGTTGCAACAATTTTAGTTATCTTCATGAATGCTGGTTTCGCTATGGTAGAAGCCGGTATGTGTAGATCTAAAAATGC
TGTCAACATTCTCGCCAAAAACCTTTTTGTATTTGCTTTAGCAGTTACTTCATATTGGTTTATTGGATTTGGAATTATGT
ATTACGGAAAGGAAATAATTCCTGGAATACTTTATTTTAATTCCTTATTCTTTGATCCAACAGTTACTGCTGAAATGATT
GAAGGAGCAGAATTAGTACCAACAGTTGACTTCCTCTTCCAAGCAGCTTTTGCTGGAACTGCAGCAACTATTGTTTCTGG
TCTCGTCGCAGAAAGAGTTAAGTTTGGAGAATTTGTTGTATTTGCCATCGTTTTAACTGCTTTCATTTACCCAATAGCTG
GTAGTTGGAAGTGGAATGGTGGTTGGCTTGATCAGCTTGGATTTATTGATTTTGCAGGCTCTTCAATTGTTCACTCAGTT
GGAGCTTGGGCAGGTCTTGTTGGAGCTATGCTTCTTGGACCAAGAATTGGTAAATACTCAGATGGAAAGCCTCAGGCTAT
GCCTGGACATAATATGGCAATTGCTACTTTAGGAGCTCTTGTTCTATGGATAGGTTGGTATGGATTTAATCCAGGTTCTC
AACTTGCTATGGATCAATGGGTACCTTACGTTGCTGTAACTACCACTTTGGCAGCAGCAGCCGGAGCTATTGGAGCAACT
ATTGTTTCTACTTTAACATCAGGAAAACCTGATCTTACAATGATCATTAATGGCATCCTTGCCGGTTTAGTAAGTATCAC
TGCCGGTTGTGGTGATATGACTCTTGTAGGAGCATGGTTCGCTGGACTAGTTGGTGGAATTATTGTCGTATTTTCTGTTG
CCGCTCTTGATGCAGCTGAAATTGATGATCCAGTAGGTGCATTTTCTGTTCACGGAGTTTGTGGCGTATGGGGAACCATA
GTCATTGGTCTTTGGGGAACTGAAATGCAAGGAAGTGGACTAGGTATTGGACTACTTAATGGTGGAGGTATTAAATTACT
TCTTGTTCAAGCACTTGGTGCAGCAGCTTATGCTATCTGGACTCTTGTTACCTGTTGGATTGCTTGGTCTGTAATTGGAG
GATTATTCGGTGGAATCCGAGTATCTGAAGAGGAAGAGACTCAAGGTCTTGATATAGGAGAGCACGGAATGGAAGCATAT
CCAGACTTTGCATCTGCTAAATAA

Upstream 100 bases:

>100_bases
TTGGTAGCAACGACTACTAGACACTAATAAGTTTTTTGAGCAAAATTGAATATGAAAGGAAACAGAACAAACTGTTTTTT
TGCAGATCTAATTTTTTTTT

Downstream 100 bases:

>100_bases
TCTAACTTAAAATTAATTTTAGAAACCTGACTTATGTCAGGTTTCTTTTTTTTTACAAAAAATTATTTAAAATGTTGTAA
TATTTAAAGATGGATACTCA

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MTTALQTPQRRSRTRLQDASLVNGPMLLLRSIRGFSSNRSMLWLATVPLALFGLGLFNVAAHATELPDLNAAFLANNLWL
FVATILVIFMNAGFAMVEAGMCRSKNAVNILAKNLFVFALAVTSYWFIGFGIMYYGKEIIPGILYFNSLFFDPTVTAEMI
EGAELVPTVDFLFQAAFAGTAATIVSGLVAERVKFGEFVVFAIVLTAFIYPIAGSWKWNGGWLDQLGFIDFAGSSIVHSV
GAWAGLVGAMLLGPRIGKYSDGKPQAMPGHNMAIATLGALVLWIGWYGFNPGSQLAMDQWVPYVAVTTTLAAAAGAIGAT
IVSTLTSGKPDLTMIINGILAGLVSITAGCGDMTLVGAWFAGLVGGIIVVFSVAALDAAEIDDPVGAFSVHGVCGVWGTI
VIGLWGTEMQGSGLGIGLLNGGGIKLLLVQALGAAAYAIWTLVTCWIAWSVIGGLFGGIRVSEEEETQGLDIGEHGMEAY
PDFASAK

Sequences:

>Translated_487_residues
MTTALQTPQRRSRTRLQDASLVNGPMLLLRSIRGFSSNRSMLWLATVPLALFGLGLFNVAAHATELPDLNAAFLANNLWL
FVATILVIFMNAGFAMVEAGMCRSKNAVNILAKNLFVFALAVTSYWFIGFGIMYYGKEIIPGILYFNSLFFDPTVTAEMI
EGAELVPTVDFLFQAAFAGTAATIVSGLVAERVKFGEFVVFAIVLTAFIYPIAGSWKWNGGWLDQLGFIDFAGSSIVHSV
GAWAGLVGAMLLGPRIGKYSDGKPQAMPGHNMAIATLGALVLWIGWYGFNPGSQLAMDQWVPYVAVTTTLAAAAGAIGAT
IVSTLTSGKPDLTMIINGILAGLVSITAGCGDMTLVGAWFAGLVGGIIVVFSVAALDAAEIDDPVGAFSVHGVCGVWGTI
VIGLWGTEMQGSGLGIGLLNGGGIKLLLVQALGAAAYAIWTLVTCWIAWSVIGGLFGGIRVSEEEETQGLDIGEHGMEAY
PDFASAK
>Mature_486_residues
TTALQTPQRRSRTRLQDASLVNGPMLLLRSIRGFSSNRSMLWLATVPLALFGLGLFNVAAHATELPDLNAAFLANNLWLF
VATILVIFMNAGFAMVEAGMCRSKNAVNILAKNLFVFALAVTSYWFIGFGIMYYGKEIIPGILYFNSLFFDPTVTAEMIE
GAELVPTVDFLFQAAFAGTAATIVSGLVAERVKFGEFVVFAIVLTAFIYPIAGSWKWNGGWLDQLGFIDFAGSSIVHSVG
AWAGLVGAMLLGPRIGKYSDGKPQAMPGHNMAIATLGALVLWIGWYGFNPGSQLAMDQWVPYVAVTTTLAAAAGAIGATI
VSTLTSGKPDLTMIINGILAGLVSITAGCGDMTLVGAWFAGLVGGIIVVFSVAALDAAEIDDPVGAFSVHGVCGVWGTIV
IGLWGTEMQGSGLGIGLLNGGGIKLLLVQALGAAAYAIWTLVTCWIAWSVIGGLFGGIRVSEEEETQGLDIGEHGMEAYP
DFASAK

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=411, Percent_Identity=34.0632603406326, Blast_Score=182, Evalue=4e-47,
Organism=Caenorhabditis elegans, GI32564042, Length=433, Percent_Identity=33.4872979214781, Blast_Score=203, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI133931100, Length=426, Percent_Identity=32.1596244131455, Blast_Score=193, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI17550182, Length=433, Percent_Identity=31.6397228637413, Blast_Score=184, Evalue=8e-47,
Organism=Caenorhabditis elegans, GI17550180, Length=460, Percent_Identity=32.3913043478261, Blast_Score=182, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6324187, Length=428, Percent_Identity=28.7383177570093, Blast_Score=135, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6321559, Length=413, Percent_Identity=28.0871670702179, Blast_Score=126, Evalue=8e-30,
Organism=Saccharomyces cerevisiae, GI6325396, Length=415, Percent_Identity=26.9879518072289, Blast_Score=126, Evalue=8e-30,
Organism=Drosophila melanogaster, GI161078314, Length=424, Percent_Identity=36.0849056603774, Blast_Score=216, Evalue=2e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 51275; Mature: 51144

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCC
>Mature Secondary Structure 
TTALQTPQRRSRTRLQDASLVNGPMLLLRSIRGFSSNRSMLWLATVPLALFGLGLFNVA
CCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHH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CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]