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The map label for this gene is trmB

Identifier: 78778627

GI number: 78778627

Start: 234620

End: 235249

Strand: Direct

Name: trmB

Synonym: PMT9312_0242

Alternate gene names: 78778627

Gene position: 234620-235249 (Clockwise)

Preceding gene: 78778625

Following gene: 78778629

Centisome position: 13.73

GC content: 25.71

Gene sequence:

>630_bases
ATGAGACAGCATGTAAATCCACTTAGTAGAAATTTCAATGAAATTGAAAGAATACCTGCTTTGATTGAAATGTTTGGTGA
TTCTAAATCTAATCTTCATTTGGATATAGGTTGTGCCGCTGGTGAGTTTCTATTCGATTTAGCCTTAATTAATACCAATT
GGAATTATATAGGAATTGAAATCCGTGAGAGATTAGTTAAAACTGCCAAATTAAAAGTGCGAGAAAGAGATATTAAAAAT
TTATATTTTATATTTGGTAATGCTAATAACATTTTGCATGATGTCCAAAGTAAATTTATTTTCAAAAACGTAAAAAGTAT
TTCTTTTAATTTTCCTGATCCATGGTTTAAAAAAAGACATTATAAAAGACGTGTAATTCAGCCAGAGTTTCTTAATCTTC
TCTCAAATTCATTGCAAAAAGGATCGCTAATTTTTATAAAAACTGATGTAAAGGATTTATTCGATTATATGGATTGCATC
ATATCAAATAATTTTAATTTTAAAAAAATAGATAAAAATGATTTTAATTTTTCTGAAAGTTTTAATCCAAGTCAAGTTAA
AACTAATAGGGAAAAGTATGTAATTGTTAACCAACTTGATATTTTTGAGAGGATTTATATAAAAATATGA

Upstream 100 bases:

>100_bases
AATCATTAAACATCAAACTGTTACTTTTTATCTTACTATTGTCATAGTTGTGAATATTTTGCATCTTTATATTATTGCCG
AGAAATAAATTAATAATTGT

Downstream 100 bases:

>100_bases
TTCATAATTAATTCATTATTTTATTAATTTCTAAAATGAGTTTGTTGGCTATTTCGCTTGATAAAGAATCAACTTTCTTA
TGGCTTTTGGCCTCAACAAG

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MRQHVNPLSRNFNEIERIPALIEMFGDSKSNLHLDIGCAAGEFLFDLALINTNWNYIGIEIRERLVKTAKLKVRERDIKN
LYFIFGNANNILHDVQSKFIFKNVKSISFNFPDPWFKKRHYKRRVIQPEFLNLLSNSLQKGSLIFIKTDVKDLFDYMDCI
ISNNFNFKKIDKNDFNFSESFNPSQVKTNREKYVIVNQLDIFERIYIKI

Sequences:

>Translated_209_residues
MRQHVNPLSRNFNEIERIPALIEMFGDSKSNLHLDIGCAAGEFLFDLALINTNWNYIGIEIRERLVKTAKLKVRERDIKN
LYFIFGNANNILHDVQSKFIFKNVKSISFNFPDPWFKKRHYKRRVIQPEFLNLLSNSLQKGSLIFIKTDVKDLFDYMDCI
ISNNFNFKKIDKNDFNFSESFNPSQVKTNREKYVIVNQLDIFERIYIKI
>Mature_209_residues
MRQHVNPLSRNFNEIERIPALIEMFGDSKSNLHLDIGCAAGEFLFDLALINTNWNYIGIEIRERLVKTAKLKVRERDIKN
LYFIFGNANNILHDVQSKFIFKNVKSISFNFPDPWFKKRHYKRRVIQPEFLNLLSNSLQKGSLIFIKTDVKDLFDYMDCI
ISNNFNFKKIDKNDFNFSESFNPSQVKTNREKYVIVNQLDIFERIYIKI

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=159, Percent_Identity=30.188679245283, Blast_Score=74, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_PROM9 (Q31CU2)

Other databases:

- EMBL:   CP000111
- RefSeq:   YP_396739.1
- ProteinModelPortal:   Q31CU2
- SMR:   Q31CU2
- STRING:   Q31CU2
- GeneID:   3765028
- GenomeReviews:   CP000111_GR
- KEGG:   pmi:PMT9312_0242
- eggNOG:   COG0220
- HOGENOM:   HBG284653
- OMA:   KRRVVQP
- ProtClustDB:   PRK00121
- BioCyc:   PMAR74546:PMT9312_0242-MONOMER
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 24841; Mature: 24841

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: NA

Important sites: BINDING 35-35 BINDING 60-60 BINDING 87-87 BINDING 113-113 BINDING 117-117 BINDING 149-149

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQHVNPLSRNFNEIERIPALIEMFGDSKSNLHLDIGCAAGEFLFDLALINTNWNYIGIE
CCCCCCHHHCCHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHEEEEEECCCCEEEHH
IRERLVKTAKLKVRERDIKNLYFIFGNANNILHDVQSKFIFKNVKSISFNFPDPWFKKRH
HHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHEECCCCCHHHHHHH
YKRRVIQPEFLNLLSNSLQKGSLIFIKTDVKDLFDYMDCIISNNFNFKKIDKNDFNFSES
HHHHCCCHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCC
FNPSQVKTNREKYVIVNQLDIFERIYIKI
CCCCHHCCCCCEEEEEEHHHHHHHHEECC
>Mature Secondary Structure
MRQHVNPLSRNFNEIERIPALIEMFGDSKSNLHLDIGCAAGEFLFDLALINTNWNYIGIE
CCCCCCHHHCCHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHEEEEEECCCCEEEHH
IRERLVKTAKLKVRERDIKNLYFIFGNANNILHDVQSKFIFKNVKSISFNFPDPWFKKRH
HHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHEECCCCCHHHHHHH
YKRRVIQPEFLNLLSNSLQKGSLIFIKTDVKDLFDYMDCIISNNFNFKKIDKNDFNFSES
HHHHCCCHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCC
FNPSQVKTNREKYVIVNQLDIFERIYIKI
CCCCHHCCCCCEEEEEEHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA